Abstract：
:Secretion of chemokines under different conditions of monocyte and endothelial cell coculturing was compared. Secretion of all the studied chemokines was recorded in cocultures: IL-8/CXCL-8, MCP-1/CCL2, RANTES/CCL5, and IP-10/CXCL10. The presence of TNF-α increased the concentrations of all chemokines, the concentrations of IL-8/CXCL-8, MCP-1/CCL2, and IP-10/CXCL10 decreased significantly in transendothelial migration. Addition of IFN-γ to cocultures significantly increased only IP-10/CXCL10 concentration; in transendothelial migration, the concentration P-10/CXCL10 decreased, while the concentrations of RANTES/CCL5 and MCP-1/CCL2 increased. Cell coculturing with IL-4 reduced the concentrations of all chemokines; the concentration of RANTES/CCL5 significantly increased in transendothelial migration. These results demonstrate the important role of monocyte-endothelial interactions in the regulation of the constitutive and cytokine-induced secretion of chemokines.
journal_name
Bull Exp Biol Medjournal_title
Bulletin of experimental biology and medicineauthors
Starickova EA,Sokolov DI,Selkov SA,Freidlin ISdoi
10.1007/s10517-011-1165-7subject
Has Abstractpub_date
2011-02-01 00:00:00pages
446-9issue
4eissn
0007-4888issn
1573-8221journal_volume
150pub_type
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