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Genome-wide identification of microRNA targets in human ES cells reveals a role for miR-302 in modulating BMP response.

Abstract：

:MicroRNAs are important regulators in many cellular processes, including stem cell self-renewal. Recent studies demonstrated their function as pluripotency factors with the capacity for somatic cell reprogramming. However, their role in human embryonic stem (ES) cells (hESCs) remains poorly understood, partially due to the lack of genome-wide strategies to identify their targets. Here, we performed comprehensive microRNA profiling in hESCs and in purified neural and mesenchymal derivatives. Using a combination of AGO cross-linking and microRNA perturbation experiments, together with computational prediction, we identified the targets of the miR-302/367 cluster, the most abundant microRNAs in hESCs. Functional studies identified novel roles of miR-302/367 in maintaining pluripotency and regulating hESC differentiation. We show that in addition to its role in TGF-β signaling, miR-302/367 promotes bone morphogenetic protein (BMP) signaling by targeting BMP inhibitors TOB2, DAZAP2, and SLAIN1. This study broadens our understanding of microRNA function in hESCs and is a valuable resource for future studies in this area.

journal_name

Genes Dev

journal_title

Genes & development

authors

Lipchina I,Elkabetz Y,Hafner M,Sheridan R,Mihailovic A,Tuschl T,Sander C,Studer L,Betel D

doi

10.1101/gad.17221311

subject

Has Abstract

pub_date

2011-10-15 00:00:00

pages

2173-86

issue

20

eissn

0890-9369

issn

1549-5477

pii

25/20/2173

journal_volume

25

pub_type

杂志文章

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