Abstract:
:The repression of the maternally inherited Igf2 allele has been proposed to depend on a methylation-sensitive chromatin insulator organized by the 11 zinc finger protein CTCF at the H19 imprinting control region (ICR). Here we document that point mutations of the nucleotides in physical contact with CTCF within the endogenous H19 ICR lead to loss of CTCF binding and Igf2 imprinting only when passaged through the female germline. This effect is accompanied by a significant loss of methylation protection of the maternally derived H19 ICR. Because CTCF interacts with other imprinting control regions, it emerges as a central factor responsible for interpreting and propagating gamete-derived epigenetic marks and for organizing epigenetically controlled expression domains.
journal_name
Genes Devjournal_title
Genes & developmentauthors
Pant V,Mariano P,Kanduri C,Mattsson A,Lobanenkov V,Heuchel R,Ohlsson Rdoi
10.1101/gad.254903subject
Has Abstractpub_date
2003-03-01 00:00:00pages
586-90issue
5eissn
0890-9369issn
1549-5477journal_volume
17pub_type
杂志文章abstract::The v-erbA oncogene of avian erythroblastosis virus (AEV) encodes a ligand-independent mutated version of the chicken c-erbA alpha-encoded thyroid hormone receptor. The v-erbA gene product, a 75-kD gag/v-erbA fusion protein, is phosphorylated on Ser-16/17 of its v-erbA-encoded domain, and phosphorylation at this site ...
journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.4.10.1663
更新日期:1990-10-01 00:00:00
abstract::Transcriptionally silent genes must be activated throughout development. This requires nucleosomes be removed from promoters and enhancers to allow transcription factor (TF) binding and recruitment of coactivators and RNA polymerase II (Pol II). Specialized pioneer TFs bind nucleosome-wrapped DNA to perform this chrom...
journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.341768.120
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abstract::In petunia flowers, the loci an1, an2, and an11 control the pigmentation of the flower by stimulating the transcription of anthocyanin biosynthetic genes. The an1 and an2 locus were recently cloned and encode a basic helix-loop-helix (bHLH) and MYB-domain transcriptional activator, respectively. Here, we report the is...
journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.11.11.1422
更新日期:1997-06-01 00:00:00
abstract::MicF, one of the first chromosomally encoded regulatory small RNAs (sRNAs) to be discovered, was found to modulate the expression of OmpF, an abundant outer membrane protein. Several recent papers have now shown that this is not an isolated case. At least five other sRNAs also regulate the synthesis of outer membrane ...
journal_title:Genes & development
pub_type: 杂志文章,评审
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abstract::The mammalian ATF/CREB family of transcription factors comprises a large group of basic-region leucine zipper (bZIP) proteins whose members mediate diverse transcriptional regulatory functions. Here we report that expression of a specific mouse ATF gene, ATFx, is down-regulated in a variety of cells undergoing apoptos...
journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.992202
更新日期:2002-07-15 00:00:00
abstract::Transcriptional repressors play a key role in establishing localized patterns of gene expression in the early Drosophila embryo. Several different modes of repression have been implicated in previous studies, including competition and direct interference with the transcription complex. Here, we present evidence for "q...
journal_title:Genes & development
pub_type: 杂志文章
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journal_title:Genes & development
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更新日期:2000-03-15 00:00:00
abstract::The human RNA polymerase II (RNAPII)-associated factor complex (hPAFc) and its individual subunits have been implicated in human diseases, including cancer. However, its involvement in breast cancer awaits investigation. Using data mining and human breast cancer tissue microarrays, we found that Ctr9, the key scaffold...
journal_title:Genes & development
pub_type: 杂志文章
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journal_title:Genes & development
pub_type: 杂志文章
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journal_title:Genes & development
pub_type: 杂志文章
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更新日期:2009-11-15 00:00:00
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journal_title:Genes & development
pub_type: 杂志文章
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更新日期:2020-01-01 00:00:00
abstract::In the cytoplasm of oocytes and early embryos, addition of poly(A) to mRNAs can activate their translation. We demonstrate that despite many differences between poly(A) addition in the cytoplasm and nucleus, these two forms of polyadenylation may involve identical trans-acting factors. Nuclear polyadenylation requires...
journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.8.9.1106
更新日期:1994-05-01 00:00:00
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journal_title:Genes & development
pub_type: 评论,杂志文章
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更新日期:2011-11-15 00:00:00
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journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.1009002
更新日期:2002-09-15 00:00:00
abstract::Historically, biomedical research has been based on two paradigms. First, measurements of biological behaviors have been based on bulk assays that average over large populations. Second, these behaviors have then been crudely perturbed by systemic administration of therapeutic treatments. Nanotechnology has the potent...
journal_title:Genes & development
pub_type: 杂志文章,评审
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journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.4.1.43
更新日期:1990-01-01 00:00:00
abstract::Nuclear export factor 1 (NXF1) exports mRNA to the cytoplasm after recruitment to mRNA by specific adaptor proteins. How and why cells use numerous different export adaptors is poorly understood. Here we critically evaluate members of the SR protein family (SRSF1-7) for their potential to act as NXF1 adaptors that cou...
journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.276477.115
更新日期:2016-03-01 00:00:00
abstract::The incorporation of histone H1 into chromatin during embryogenesis directs the specific repression of the Xenopus oocyte 5S rRNA genes. An increase in histone H1 content specifically restricts TFIIIA-activated transcription, and a decrease in histone H1 within chromatin facilitates the activation of the oocyte 5S rRN...
journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.8.10.1147
更新日期:1994-05-15 00:00:00
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journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.1546307
更新日期:2007-05-15 00:00:00
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journal_title:Genes & development
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更新日期:2005-06-01 00:00:00
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journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.271528.115
更新日期:2015-12-15 00:00:00
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journal_title:Genes & development
pub_type: 杂志文章
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journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.322818.118
更新日期:2019-05-01 00:00:00
abstract::Smads regulate transcription of defined genes in response to TGF-beta receptor activation, although the mechanisms of Smad-mediated transcription are not well understood. We demonstrate that the TGF-beta-inducible Smad3 uses the tumor suppressor Smad4/DPC4 and CBP/p300 as transcriptional coactivators, which associate ...
journal_title:Genes & development
pub_type: 杂志文章
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journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.1317105
更新日期:2005-08-01 00:00:00
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journal_title:Genes & development
pub_type: 杂志文章
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journal_title:Genes & development
pub_type: 杂志文章
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journal_title:Genes & development
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更新日期:2017-09-01 00:00:00
abstract::We have shown that Wnt signals are necessary and sufficient for neural crest cells to adopt pigment cell fates. nacre, a zebrafish homolog of MITF, is required for pigment cell differentiation. We isolated a promoter region of nacre that contains Tcf/Lef binding sites, which can mediate Wnt responsiveness. This promot...
journal_title:Genes & development
pub_type: 杂志文章
doi:
更新日期:2000-01-15 00:00:00