Abstract：
:Transcriptionally silent genes must be activated throughout development. This requires nucleosomes be removed from promoters and enhancers to allow transcription factor (TF) binding and recruitment of coactivators and RNA polymerase II (Pol II). Specialized pioneer TFs bind nucleosome-wrapped DNA to perform this chromatin opening by mechanisms that remain incompletely understood. Here, we show that GAGA factor (GAF), a Drosophila pioneer-like factor, functions with both SWI/SNF and ISWI family chromatin remodelers to allow recruitment of Pol II and entry to a promoter-proximal paused state, and also to promote Pol II's transition to productive elongation. We found that GAF interacts with PBAP (SWI/SNF) to open chromatin and allow Pol II to be recruited. Importantly, this activity is not dependent on NURF as previously proposed; however, GAF also synergizes with NURF downstream from this process to ensure efficient Pol II pause release and transition to productive elongation, apparently through its role in precisely positioning the +1 nucleosome. These results demonstrate how a single sequence-specific pioneer TF can synergize with remodelers to activate sets of genes. Furthermore, this behavior of remodelers is consistent with findings in yeast and mice, and likely represents general, conserved mechanisms found throughout eukarya.
journal_name
Genes Devjournal_title
Genes & developmentauthors
Judd J,Duarte FM,Lis JTdoi
10.1101/gad.341768.120subject
Has Abstractpub_date
2021-01-01 00:00:00pages
147-156issue
1-2eissn
0890-9369issn
1549-5477pii
gad.341768.120journal_volume
35pub_type
杂志文章abstract：:The oncoprotein MDM2 binds to the activation domain of the tumor suppressor p53 and inhibits its ability to stimulate transcription. This same region of p53 is able to bind several basal transcription factors that appear to be important for the transactivation function of p53. It has therefore been suggested that MDM2...
journal_title：Genes & development
pub_type： 杂志文章
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journal_title：Genes & development
pub_type： 杂志文章
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更新日期：2015-12-01 00:00:00
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pub_type： 杂志文章,评审
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journal_title：Genes & development
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更新日期：2011-03-01 00:00:00
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更新日期：2015-04-15 00:00:00
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journal_title：Genes & development
pub_type： 杂志文章
doi：
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