Abstract:
:We generated and characterized greater than 500 Drosophila strains that carry single copies of a novel P-element enhancer detector. In the majority of the strains, the beta-galactosidase reporter gene in the P-transposon responds to nearby transcriptional regulatory sequences in the genome. A remarkable diversity of spatially and temporally regulated staining patterns is observed in embryos carrying different insertions. We selected numerous strains as markers for different embryonic organs, tissues, and cells. Many of these strains should allow the study of complex developmental processes, such as nervous system development, which have not been convenient to analyze previously. Also, we present genetic evidence that some of the detected regulatory elements control nearby Drosophila genes. In light of our results, we discuss the diversity and complexity of cis-acting regulatory elements in the genome and the general applications of the enhancer detector method for the study of Drosophila development.
journal_name
Genes Devjournal_title
Genes & developmentauthors
Bellen HJ,O'Kane CJ,Wilson C,Grossniklaus U,Pearson RK,Gehring WJdoi
10.1101/gad.3.9.1288subject
Has Abstractpub_date
1989-09-01 00:00:00pages
1288-300issue
9eissn
0890-9369issn
1549-5477journal_volume
3pub_type
杂志文章abstract::Chromatin is organized into higher-order structures that form subcompartments in interphase nuclei. Different categories of specialized enzymes act on chromatin and regulate its compaction and biophysical characteristics in response to physiological conditions. We present an overview of the function of chromatin struc...
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pub_type: 杂志文章,评审
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journal_title:Genes & development
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journal_title:Genes & development
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journal_title:Genes & development
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更新日期:2008-09-15 00:00:00
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journal_title:Genes & development
pub_type: 杂志文章
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更新日期:2005-12-01 00:00:00
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journal_title:Genes & development
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更新日期:2010-08-15 00:00:00
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journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.10.8.921
更新日期:1996-04-15 00:00:00
abstract::A long-standing model postulates that X-chromosome dosage compensation in Drosophila occurs by twofold up-regulation of the single male X, but previous data cannot exclude an alternative model, in which male autosomes are down-regulated to balance gene expression. To distinguish between the two models, we used RNA int...
journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.1343705
更新日期:2005-10-01 00:00:00
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journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.190413.112
更新日期:2012-09-01 00:00:00
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journal_title:Genes & development
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journal_title:Genes & development
pub_type: 杂志文章
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更新日期:1989-09-01 00:00:00
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journal_title:Genes & development
pub_type: 杂志文章
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更新日期:1997-11-15 00:00:00
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journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.1373705
更新日期:2005-12-15 00:00:00
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journal_title:Genes & development
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doi:10.1101/gad.455407
更新日期:2007-12-15 00:00:00
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更新日期:1998-02-01 00:00:00
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journal_title:Genes & development
pub_type: 杂志文章
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更新日期:2019-11-01 00:00:00