Abstract:
:Genome integrity is constantly monitored by sophisticated cellular networks, collectively termed the DNA damage response (DDR). A common feature of DDR proteins is their mobilization in response to genotoxic stress. Here, we outline how the development of various complementary methodologies has provided valuable insights into the spatiotemporal dynamics of DDR protein assembly/disassembly at sites of DNA strand breaks in eukaryotic cells. Considerable advances have also been made in understanding the underlying molecular mechanisms for these events, with post-translational modifications of DDR factors being shown to play prominent roles in controlling the formation of foci in response to DNA-damaging agents. We review these regulatory mechanisms and discuss their biological significance to the DDR.
journal_name
Genes Devjournal_title
Genes & developmentauthors
Polo SE,Jackson SPdoi
10.1101/gad.2021311subject
Has Abstractpub_date
2011-03-01 00:00:00pages
409-33issue
5eissn
0890-9369issn
1549-5477pii
25/5/409journal_volume
25pub_type
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