Abstract:
:The widespread class of RNA viruses that utilize internal ribosome entry sites (IRESs) for translation include poliovirus and Hepatitis C virus. To identify host factors required for IRES-dependent translation and viral replication, we performed a genome-wide RNAi screen in Drosophila cells infected with Drosophila C virus (DCV). We identified 66 ribosomal proteins that, when depleted, specifically inhibit DCV growth, but not a non-IRES-containing RNA virus. Moreover, treatment of flies with a translation inhibitor is protective in vivo. Finally, this increased sensitivity to ribosome levels also holds true for poliovirus infection of human cells, demonstrating the generality of these findings.
journal_name
Genes Devjournal_title
Genes & developmentauthors
Cherry S,Doukas T,Armknecht S,Whelan S,Wang H,Sarnow P,Perrimon Ndoi
10.1101/gad.1267905subject
Has Abstractpub_date
2005-02-15 00:00:00pages
445-52issue
4eissn
0890-9369issn
1549-5477pii
19/4/445journal_volume
19pub_type
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