Genome-wide RNAi screen reveals a specific sensitivity of IRES-containing RNA viruses to host translation inhibition.

Abstract:

:The widespread class of RNA viruses that utilize internal ribosome entry sites (IRESs) for translation include poliovirus and Hepatitis C virus. To identify host factors required for IRES-dependent translation and viral replication, we performed a genome-wide RNAi screen in Drosophila cells infected with Drosophila C virus (DCV). We identified 66 ribosomal proteins that, when depleted, specifically inhibit DCV growth, but not a non-IRES-containing RNA virus. Moreover, treatment of flies with a translation inhibitor is protective in vivo. Finally, this increased sensitivity to ribosome levels also holds true for poliovirus infection of human cells, demonstrating the generality of these findings.

journal_name

Genes Dev

journal_title

Genes & development

authors

Cherry S,Doukas T,Armknecht S,Whelan S,Wang H,Sarnow P,Perrimon N

doi

10.1101/gad.1267905

subject

Has Abstract

pub_date

2005-02-15 00:00:00

pages

445-52

issue

4

eissn

0890-9369

issn

1549-5477

pii

19/4/445

journal_volume

19

pub_type

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