Abstract:
:Smads regulate transcription of defined genes in response to TGF-beta receptor activation, although the mechanisms of Smad-mediated transcription are not well understood. We demonstrate that the TGF-beta-inducible Smad3 uses the tumor suppressor Smad4/DPC4 and CBP/p300 as transcriptional coactivators, which associate with Smad3 in response to TGF-beta. The association of CBP with Smad3 was localized to the carboxyl terminus of Smad3, which is required for transcriptional activation, and a defined segment in CBP. Furthermore, CBP/p300 stimulated both TGF-beta- and Smad-induced transcription in a Smad4/DPC4-dependent fashion. Smad3 transactivation and TGF-beta-induced transcription were inhibited by expressing E1A, which interferes with CBP functions. The coactivator functions and physical interactions of Smad4 and CBP/p300 with Smad3 allow a model for the induction of gene expression in response to TGF-beta.
journal_name
Genes Devjournal_title
Genes & developmentauthors
Feng XH,Zhang Y,Wu RY,Derynck Rdoi
10.1101/gad.12.14.2153subject
Has Abstractpub_date
1998-07-15 00:00:00pages
2153-63issue
14eissn
0890-9369issn
1549-5477journal_volume
12pub_type
杂志文章abstract::The X-inactive-specific transcript (Xist) locus is a cis-acting switch that regulates X chromosome inactivation in mammals. Over recent years an important goal has been to understand how Xist is regulated at the initiation of X inactivation. Here we report the analysis of a series of targeted mutations at the 5' end o...
journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.271203
更新日期:2003-09-01 00:00:00
abstract::An insulin receptor-like signaling pathway regulates Caenorhabditis elegans metabolism, development, and longevity. Inactivation of the insulin receptor homolog DAF-2, the AGE-1 PI3K, or the AKT-1 and AKT-2 kinases causes a developmental arrest at the dauer stage. A null mutation in the daf-16 Fork head transcription ...
journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.13.11.1438
更新日期:1999-06-01 00:00:00
abstract::Inactivation of cyclin B-Cdc2 kinase at the exit from M phase depends on the specific proteolysis of the cyclin B subunit, whereas the Cdc2 subunit remains present at nearly constant levels throughout the cell cycle. It is unknown how Cdc2 escapes degradation when cyclin B is destroyed. In Xenopus egg extracts that re...
journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.823200
更新日期:2000-09-15 00:00:00
abstract::Human lymphoblastoid cells derived from different healthy individuals display considerable variation in their transcription profiles. Here we show that such variation in gene expression underlies interindividual susceptibility to DNA damaging agents. The results demonstrate the massive differences in sensitivity acros...
journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.1688508
更新日期:2008-10-01 00:00:00
abstract::A core promoter element called an initiator (Inr) overlaps the transcription start site of numerous mammalian protein-coding genes. In promoters that lack a TATA box, the Inr is functionally analogous to TATA, in that it is capable of directing basal transcription by RNA polymerase II and of determining the precise si...
journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.8.7.821
更新日期:1994-04-01 00:00:00
abstract::Far1p is a bifunctional protein that is required to arrest the cell cycle and to establish cell polarity during yeast mating. Far1p is localized predominantly in the nucleus but accumulates in the cytoplasm in cells exposed to pheromones. Here we show that Far1p functions in both subcellular compartments: nuclear Far1...
journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.13.17.2284
更新日期:1999-09-01 00:00:00
abstract::We have used the 100-kD HeLa cell octamer-binding protein OBP100 as a model to study flexible DNA sequence recognition by promoter-binding proteins. OBP100 binds to the conserved octamer motif ATGCAAAT found in numerous promoters and additionally to two degenerate octamer motifs (sites I and II) within the SV40 enhanc...
journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.2.11.1400
更新日期:1988-11-01 00:00:00
abstract::The ATR (ATM and Rad3-related) kinase and its regulatory partner ATRIP (ATR-interacting protein) coordinate checkpoint responses to DNA damage and replication stress. TopBP1 functions as a general activator of ATR. However, the mechanism by which TopBP1 activates ATR is unknown. Here, we show that ATRIP contains a Top...
journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.1666208
更新日期:2008-06-01 00:00:00
abstract::Somitogenesis involves the segmentation of the paraxial mesoderm into units along the anteroposterior axis. Here we show a role for Eph and ephrin signaling in the patterning of presomitic mesoderm and formation of the somites. Ephrin-A-L1 and ephrin-B2 are expressed in an iterative manner in the developing somites an...
journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.12.19.3096
更新日期:1998-10-01 00:00:00
abstract::Dosage compensation in Drosophila occurs by a twofold increase in transcription per copy of X-linked genes in males (XY) compared with females (XX). msl-1 is one of four genes that are essential for dosage compensation in males, and MSL-1 protein is associated specifically with the male X chromosome. To explore the ba...
journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.8.6.698
更新日期:1994-03-15 00:00:00
abstract::Covalent modifications of the histone N tails play important roles in eukaryotic gene expression. Histone acetylation, in particular, is required for the activation of a subset of eukaryotic genes through the targeted recruitment of histone acetyltransferases. We have reported that a histone C tail modification, ubiqu...
journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.1149604
更新日期:2004-01-15 00:00:00
abstract::Asymmetric localization of cell fate determinants is a crucial step in neuroblast asymmetric divisions. Whereas several protein kinases have been shown to mediate this process, no protein phosphatase has so far been implicated. In a clonal screen of larval neuroblasts we identified the evolutionarily conserved Protein...
journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.1723609
更新日期:2009-02-01 00:00:00
abstract::To investigate the importance of sequences between the yeast (Saccharomyces cerevisiae) branch point (TACTAAC box) and 3' splice site (AG), we generated a series of pre-mRNA substrates that differed in the length of RNA retained on the 3' side of the TACTAAC box. These pre-mRNAs were compared as substrates for the fir...
journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.1.3.238
更新日期:1987-05-01 00:00:00
abstract::The crucifer Arabidopsis thaliana has been used widely as a model organism for the study of plant development. We describe here the development of an efficient insertional mutagenesis system in Arabidopsis that permits identification of genes by their patterns of expression during development. Transposable elements of...
journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.9.14.1797
更新日期:1995-07-15 00:00:00
abstract::The Swi/Snf chromatin remodeling complex functions to alter nucleosome positions by either sliding nucleosomes on DNA or the eviction of histones. The presence of histone acetylation and activator-dependent recruitment and retention of Swi/Snf is important for its efficient function. It is not understood, however, why...
journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.243584.114
更新日期:2014-10-15 00:00:00
abstract::Long-range migrating progenitor cells generate hypaxial muscle, for instance the muscle of the limbs, hypoglossal cord, and diaphragm. We show here that migrating muscle progenitors express the chemokine receptor CXCR4. The corresponding ligand, SDF1, is expressed in limb and branchial arch mesenchyme; i.e., along the...
journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.346205
更新日期:2005-09-15 00:00:00
abstract::We identify the "M region" of the muscle-specific Xenopus cardiac actin gene promoter from -282 to -348 as necessary for the embryonic expression of a cardiac actin-beta-globin reporter gene injected into fertilized eggs. Four DNA-binding activities in embryo extracts, embryonic M-region factors 1-4 (EMF1-4), are desc...
journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.5.7.1149
更新日期:1991-07-01 00:00:00
abstract::Chemical mutagenesis in the mouse is a powerful approach for phenotype-driven genetics, but questions remain about the efficiency with which new mutations ascertained by their phenotype can be localized and identified, and that knowledge applied to a specific biological problem. During a global screen for dominant phe...
journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.1023703
更新日期:2003-01-15 00:00:00
abstract::Previous work has shown that rRNA synthesis is strongly inhibited in yeast top1-top2 double mutants. Here, we show that inactivation of yeast topoisomerases can have paradoxical effects on transcription by RNA polymerase I. For example, transcription of ribosomal minigenes on extrachromosomal plasmids is greatly stimu...
journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.6.7.1332
更新日期:1992-07-01 00:00:00
abstract::Lineage commitment studies in mammary glands have focused on identifying cell populations that display stem or progenitor properties. However, the mechanisms that control cell fate have been incompletely explored. Herein we show that zinc finger protein 157 (Zfp157) is required to establish the balance between luminal...
journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.184051.111
更新日期:2012-05-15 00:00:00
abstract::Gene inactivation can result when a delta element of the Ty1 transposon inserted into the yeast HIS4 promoter (his4-912delta) alters the transcription initiation site. Previous work has identified mutations that suppress this transcriptional defect by restoring the transcription start site to the native position, and ...
journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.10.5.604
更新日期:1996-03-01 00:00:00
abstract::The POU transcription factor Oct-4 is expressed specifically in the germ line, pluripotent cells of the pregastrulation embryo and stem cell lines derived from the early embryo. Osteopontin (OPN) is a protein secreted by cells of the preimplantation embryo and contains a GRGDS motif that can bind to specific integrin ...
journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.12.13.2073
更新日期:1998-07-01 00:00:00
abstract::The v-erbA oncogene of avian erythroblastosis virus (AEV) encodes a ligand-independent mutated version of the chicken c-erbA alpha-encoded thyroid hormone receptor. The v-erbA gene product, a 75-kD gag/v-erbA fusion protein, is phosphorylated on Ser-16/17 of its v-erbA-encoded domain, and phosphorylation at this site ...
journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.4.10.1663
更新日期:1990-10-01 00:00:00
abstract::The dihydrofolate reductase (DHFR) and 2BE2121 genes in the Chinese hamster are convergently transcribed in late G1 and ea ly S phase, and bracket an early-firing origin of replication that consists of a 55-kb zone of potential initiation sites. To test whether transcription through the DHFR gene is required to activa...
journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.1171404
更新日期:2004-02-15 00:00:00
abstract::Myxococcus xanthus development requires CsgA, a member of the short-chain alcohol dehydrogenase (SCAD) family of proteins. We show that CsgA and SocA, a protein that can replace CsgA function in vivo, oxidize the 2'-OH glycerol moiety on cardiolipin and phosphatidylglycerol to produce diacylglycerol (DAG), dihydroxyac...
journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.268482.115
更新日期:2015-09-15 00:00:00
abstract::Metastasis accounts for the vast majority of cancer-related deaths, yet the molecular mechanisms that drive metastatic spread remain poorly understood. Here we report that Tks5, which has been linked to the formation of proteolytic cellular protrusions known as invadopodia, undergoes an isoform switch during metastati...
journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.222745.113
更新日期:2013-07-15 00:00:00
abstract::Pre-mRNA processing is coupled with transcription. It is still unclear if the transcription machinery can also directly affect the cytoplasmic fate of a transcript, such as its intracellular localization. In yeast, the RNA-binding protein She2p binds several mRNAs and targets them for localization at the bud. Here we ...
journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.1937510
更新日期:2010-09-01 00:00:00
abstract::The conserved RCN family of proteins can bind and directly regulate calcineurin, a Ca(2+)-activated protein phosphatase involved in immunity, heart growth, muscle development, learning, and other processes. Whereas high levels of RCNs can inhibit calcineurin signaling in fungal and animal cells, RCNs can also stimulat...
journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.1159204
更新日期:2004-01-01 00:00:00
abstract::Ste5, the prototypic mitogen-activated protein kinase (MAPK) scaffold protein, associates with plasma membrane-tethered Gbetagamma freed upon pheromone receptor occupancy, thereby initiating downstream signaling. We demonstrate that this interaction and membrane binding of an N-terminal amphipathic alpha-helix (PM mot...
journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.1413706
更新日期:2006-07-15 00:00:00
abstract::The labial (lab) gene of Drosophila melanogaster is necessary for the proper development of the embryonic (larval) and adult head. We have identified the lab transcription unit within the proximal portion of the Antennapedia Complex (ANT-C) by mapping the molecular lesions associated with chromosomally rearranged lab ...
journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.3.3.399
更新日期:1989-03-01 00:00:00