Abstract:
:The crucifer Arabidopsis thaliana has been used widely as a model organism for the study of plant development. We describe here the development of an efficient insertional mutagenesis system in Arabidopsis that permits identification of genes by their patterns of expression during development. Transposable elements of the Ac/Ds system carrying the GUS reporter gene have been designed to act as enhancer traps or gene traps. A novel selection scheme maximizes recovery of unlinked transposition events. In this study 491 plants carrying independent transposon insertions were generated and screened for expression patterns. One-half of the enhancer trap insertions and one-quarter of the gene trap insertions displayed GUS expression in seedlings or flowers, including expression patterns specific to organs, tissues, cell types, or developmental stages. The patterns identify genes that act during organogenesis, pattern formation, or cell differentiation. Transposon insertion lines with specific GUS expression patterns provide valuable markers for studies of Arabidopsis development and identify new cell types or subtypes in plants. The diversity of gene expression patterns generated suggests that the identification and cloning of Arabidopsis genes expressed in any developmental process is feasible using this system.
journal_name
Genes Devjournal_title
Genes & developmentauthors
Sundaresan V,Springer P,Volpe T,Haward S,Jones JD,Dean C,Ma H,Martienssen Rdoi
10.1101/gad.9.14.1797subject
Has Abstractpub_date
1995-07-15 00:00:00pages
1797-810issue
14eissn
0890-9369issn
1549-5477journal_volume
9pub_type
杂志文章abstract::cappuccino and spire are unique Drosophila maternal-effect loci that participate in pattern formation in both the anteroposterior and dorsoventral axes of the early embryo. Mutant females produce embryos lacking pole cells, polar granules, and normal abdominal segmentation. They share these defects with the posterior ...
journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.3.9.1437
更新日期:1989-09-01 00:00:00
abstract::The unique property of stem cells to self-renew suggests specific mechanisms that regulate their cell-cycle progression. In the present study, we identify a novel protein, nucleostemin, found in the nucleoli of CNS stem cells, embryonic stem cells, and several cancer cell lines and preferentially expressed by other st...
journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.55671
更新日期:2002-12-01 00:00:00
abstract::Caenorhabditis elegans GLD-1, a KH motif containing RNA-binding protein of the GSG/STAR subfamily, controls diverse aspects of germ line development, suggesting that it may have multiple mRNA targets. We used an immunoprecipitation/subtractive hybridization/cloning strategy to identify 15 mRNAs that are putative targe...
journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.915901
更新日期:2001-09-15 00:00:00
abstract::Wingless (Wg) is an important signaling molecule in the development of Drosophila, but little is known about its signal transduction pathway. Genetic evidence indicates that another segment polarity gene, dishevelled (dsh) is required for Wg signaling. We have recently developed a cell culture system for Wg protein ac...
journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.9.9.1087
更新日期:1995-05-01 00:00:00
abstract::In Drosophila the function of the epidermal growth factor (EGF) receptor is modulated zygotically by three EGF-like proteins: Spitz (Spi), which is a potent activator; Vein (Vn), which is a moderate activator; and Argos (Aos), which is an inhibitor. Chimeric molecules were constructed in which the EGF domain of Vn was...
journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.12.7.908
更新日期:1998-04-01 00:00:00
abstract::Cellular signaling initiated by Hedgehog binding to Patched1 has profound importance in mammalian embryogenesis, genetic disease, and cancer. Hedgehog acts as a morphogen to specify distinctive cell fates using different concentration thresholds, but our knowledge of how the concentration gradient is interpreted into ...
journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.1546307
更新日期:2007-05-15 00:00:00
abstract::Distinctive from that of the animal system, the basic plan of the plant body is the continuous formation of a structural unit, composed of a stem with a meristem at the top and lateral organs continuously forming at the meristem. Therefore, mechanisms controlling the formation, maintenance, and development of a merist...
journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.13.9.1079
更新日期:1999-05-01 00:00:00
abstract::In this issue of Genes & Development, Dowen and colleagues (pp. 1515-1528) elegantly unify two previously unconnected aspects of physiology. The investigators provide significant genetic evidence to support a critical link between developmental timing decisions and the regulation of lipid mobilization at the transitio...
journal_title:Genes & development
pub_type: 评论,杂志文章
doi:10.1101/gad.285460.116
更新日期:2016-07-01 00:00:00
abstract::Start sites of DNA replication are marked by the origin recognition complex (ORC), which coordinates Mcm2-7 helicase loading to form the prereplicative complex (pre-RC). Although pre-RC assembly is well characterized in vitro, the process is poorly understood within the local chromatin environment surrounding replicat...
journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.247924.114
更新日期:2015-01-15 00:00:00
abstract::Reactive oxygen species (ROS) participate in numerous cell responses, including proliferation, DNA damage, and cell death. Based on these disparate activities, both promotion and inhibition of ROS have been proposed for cancer therapy. However, how the ROS response is determined is not clear. We examined the activitie...
journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.271130.115
更新日期:2016-01-01 00:00:00
abstract::The Saccharomyces cerevisiae protein kinase Rim15p was identified previously as a stimulator of meiotic gene expression. Here, we show that loss of Rim15p causes an additional pleiotropic phenotype in cells grown to stationary phase on rich medium; this phenotype includes defects in trehalose and glycogen accumulation...
journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.12.18.2943
更新日期:1998-09-15 00:00:00
abstract::Diverse developmental processes, such as neuronal growth cone migration and cell shape changes, are mediated by the interactions of cells with the extracellular matrix. We describe here a secreted molecule encoded by the Drosophila masquerade (mas) gene. Total loss of mas function causes defective muscle attachment. T...
journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.9.2.139
更新日期:1995-01-15 00:00:00
abstract::Post-translational modifications of histones are significant regulators of replication, transcription, and DNA repair. Particularly, newly synthesized histone H4 in H3/H4 heterodimers becomes acetylated on N-terminal lysine residues prior to its incorporation into chromatin. Previous studies have established that the ...
journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.240531.114
更新日期:2014-06-01 00:00:00
abstract::Coliphage N4 is a double-stranded DNA virus that requires the sequential activity of three different RNA polymerases during infection. The N4 virion RNA polymerase, which is carried in the virion and is injected with the DNA at the start of infection, is responsible for the synthesis of N4 early RNAs. In vitro, the vi...
journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.6.10.2010
更新日期:1992-10-01 00:00:00
abstract::Gastrulation in mice is associated with the start of extreme proliferation and differentiation. The potential cost to the embryo of a very rapid proliferation rate is a high production of damaged cells. We demonstrate a novel surveillance mechanism for the elimination of cells damaged by ionizing radiation during mous...
journal_title:Genes & development
pub_type: 杂志文章
doi:
更新日期:2000-08-15 00:00:00
abstract::The Neurospora crassa clock-controlled gene (ccg-2) is transcriptionally activated by the circadian clock in a time-of-day-specific manner. Transcript and sequence analyses of ccg-2 reveal that the predicted Ccg-2 polypeptide bears significant similarity to a class of low-molecular-weight, cysteine-rich, hydrophobic p...
journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.6.12a.2382
更新日期:1992-12-01 00:00:00
abstract::We have developed a simple, effective genetic screen for mutant alleles of eukaryotic DNA topoisomerase I that manifest severely depressed or complete loss of enzymatic function. The screen is based on the extreme toxicity of vaccinia topoisomerase expression in the Escherichia coli lysogen strain BL21(DE3) and is not...
journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.4.4.515
更新日期:1990-04-01 00:00:00
abstract::The enzyme telomerase, which elongates chromosome termini, is a critical factor in determining long-term cellular proliferation and tissue renewal. Hence, even small differences in telomerase levels can have substantial consequences for human health. In budding yeast, telomerase consists of the catalytic Est2 protein ...
journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.246256.114
更新日期:2014-10-01 00:00:00
abstract::The Elongin BC complex was identified initially as a positive regulator of RNA polymerase II (Pol II) elongation factor Elongin A and subsequently as a component of the multiprotein von Hippel-Lindau (VHL) tumor suppressor complex, in which it participates in both tumor suppression and negative regulation of hypoxia-i...
journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.12.24.3872
更新日期:1998-12-15 00:00:00
abstract::The Ultrabithorax (Ubx) gene of Drosophila melanogaster encodes a family of UBX proteins that are thought to specify the developmental fates of segments in the posterior thorax and anterior abdomen by controlling the expression of a set of target genes. UBX proteins bind DNA in vitro, and they activate or repress diff...
journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.4.6.1044
更新日期:1990-06-01 00:00:00
abstract::A transgenic mouse model for retinoblastoma was produced previously by directing SV40 T antigen expression to retinal photoreceptor cells using the promoter of the interstitial retinol-binding protein (IRBP) gene. This gene becomes active prior to the terminal differentiation of photoreceptors. Because T antigen-trans...
journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.8.11.1300
更新日期:1994-06-01 00:00:00
abstract::Maternal imprinting at the Xist gene is essential to achieve paternal allele-specific imprinted X-chromosome inactivation (XCI) in female mammals. However, the mechanism underlying Xist imprinting is unclear. Here we show that the Xist locus is coated with a broad H3K27me3 domain that is established during oocyte grow...
journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.304113.117
更新日期:2017-10-01 00:00:00
abstract::Fgf-10-deficient mice (Fgf-10(-/-)) were generated to determine the role(s) of Fgf-10 in vertebrate development. Limb bud initiation was abolished in Fgf-10(-/-) mice. Strikingly, Fgf-10(-/-) fetuses continued to develop until birth, despite the complete absence of both fore- and hindlimbs. Fgf-10 is necessary for api...
journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.12.20.3156
更新日期:1998-10-15 00:00:00
abstract::Pancreatic ductal adenocarcinoma (PDA) develops predominantly through pancreatic intraepithelial neoplasia (PanIN) and intraductal papillary mucinous neoplasm (IPMN) precursor lesions. Pancreatic acinar cells are reprogrammed to a "ductal-like" state during PanIN-PDA formation. Here, we demonstrate a parallel mechanis...
journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.256628.114
更新日期:2015-03-15 00:00:00
abstract::Sensory organ formation in Drosophila is activated by proneural genes that encode basic-helix-loop-helix (bHLH) transcription factors. These genes are antagonized by hairy and other proline-bHLH proteins. hairy has not been shown to bind to DNA and has been proposed to form inactive heterodimers with proneural activat...
journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.8.22.2743
更新日期:1994-11-15 00:00:00
abstract::Ferredoxin reductase (FDXR), a target of p53, modulates p53-dependent apoptosis and is necessary for steroidogenesis and biogenesis of iron-sulfur clusters. To determine the biological function of FDXR, we generated a Fdxr-deficient mouse model and found that loss of Fdxr led to embryonic lethality potentially due to ...
journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.299388.117
更新日期:2017-06-15 00:00:00
abstract::Mammalian telomeres are coated by the sequence-specific, DNA-binding protein, TRF1, a negative regulator of telomere length. Previous results showed that ADP-ribosylation of TRF1 by tankyrase 1 released TRF1 from telomeres and promoted telomere elongation. We now show that loss of TRF1 from telomeres results in ubiqui...
journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.1077103
更新日期:2003-06-01 00:00:00
abstract::The mechanisms that determine the relative positions of floral organs, and thereby their numbers, is a poorly understood aspect of flower development. We isolated a petunia mutant, floozy (fzy), in which the formation of floral organ primordia in the outermost three floral whorls and one of the two bracts at the base ...
journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.219502
更新日期:2002-03-15 00:00:00
abstract::Restricted expression of a mouse Vasa homolog gene (Mvh) expression is first detected in primordial germ cells (PGCs) after colonization of the genital ridges. Subsequently, Mvh is maintained until postmeiotic germ cells are formed. Here, we demonstrate that male mice homozygous for a targeted mutation of Mvh exhibit ...
journal_title:Genes & development
pub_type: 杂志文章
doi:
更新日期:2000-04-01 00:00:00
abstract::Deregulated expression of the c-myc proto-oncogene can lead to apoptosis under certain physiological conditions. By introducing a conditionally active Myc allele into primary embryo fibroblasts null for p53, and into fibroblasts without endogenous p53 expression but ectopically expressing a temperature-sensitive p53 a...
journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.8.23.2817
更新日期:1994-12-01 00:00:00