Abstract：
:The Neurospora crassa clock-controlled gene (ccg-2) is transcriptionally activated by the circadian clock in a time-of-day-specific manner. Transcript and sequence analyses of ccg-2 reveal that the predicted Ccg-2 polypeptide bears significant similarity to a class of low-molecular-weight, cysteine-rich, hydrophobic proteins (hydrophobins), first identified in Schizophyllum, and including the product of the developmentally regulated Aspergillus gene, rodletless, required for spore surface rodlets. Allelism between ccg-2 and easily wettable (eas) (one of the first developmental genetic loci identified in Neurospora) was predicted on the basis of this similarity, their close genetic linkage, and previous findings demonstrating that eas mutants lack rodlets. In this study allelism is confirmed experimentally by showing that (1) transformation of an eas mutant strain with ccg-2 DNA results in phenotypic complementation, including restoration of surface rodlets, (2) inactivation of the ccg-2 gene, by RIP, results in an eas phenotype including loss of rodlet fascicles, and (3) the original eas strain has dramatically reduced levels of ccg-2 mRNA. Thus, the clock-controlled ccg-2 gene encodes an integral component of fungal asexual spores important for spore dispersal. The dramatic reduction of ccg-2 expression in the eas mutant has no apparent effect on the normal operation of the circadian clock, confirming that there is no feedback of this clock output on the oscillator itself. These data, in conjunction with the previous observation that ccg-2 is light induced, serve to focus attention on the dual interacting role of light and the circadian clock in the regulation of fungal spore development.
journal_name
Genes Devjournal_title
Genes & developmentauthors
Bell-Pedersen D,Dunlap JC,Loros JJdoi
10.1101/gad.6.12a.2382subject
Has Abstractpub_date
1992-12-01 00:00:00pages
2382-94issue
12Aeissn
0890-9369issn
1549-5477journal_volume
6pub_type
杂志文章abstract：:Although progesterone has been recognized as essential for the establishment and maintenance of pregnancy, this steroid hormone has been recently implicated to have a functional role in a number of other reproductive events. The physiological effects of progesterone are mediated by the progesterone receptor (PR), a me...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.9.18.2266
更新日期：1995-09-15 00:00:00
abstract：:One of the most common microsatellites in eukaryotes consists of tandem arrays of the dinucleotide GT. Although the study of the instability of such repetitive DNA has been extremely fruitful over the last decade, no biological function has been demonstrated for these sequences. We investigated the genetic behavior of...
journal_title：Genes & development
pub_type： 杂志文章
doi：
更新日期：2000-05-15 00:00:00
abstract：:Histone deacetylase 3 (HDAC3) is one of four members of the human class I HDACs that regulates gene expression by deacetylation of histones and nonhistone proteins. Early studies have suggested that HDAC3 activity is regulated by association with the corepressors N-CoR and SMRT. Here we demonstrate that, in addition t...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.1286005
更新日期：2005-04-01 00:00:00
abstract：:DNA methylation is tightly regulated throughout mammalian development, and altered DNA methylation patterns are a general hallmark of cancer. The methylcytosine dioxygenase TET2 is frequently mutated in hematological disorders, including acute myeloid leukemia (AML), and has been suggested to protect CG dinucleotide (...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.260174.115
更新日期：2015-05-01 00:00:00
abstract：:Intracellular heme levels must be tightly regulated to maintain proper mitochondrial respiration while minimizing toxicity, but the homeostatic mechanisms are not well understood. Here we report a novel negative feedback mechanism whereby the nuclear heme receptor Rev-erbalpha tightly controls the level of its own lig...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.1825809
更新日期：2009-09-15 00:00:00
abstract：:MicF, one of the first chromosomally encoded regulatory small RNAs (sRNAs) to be discovered, was found to modulate the expression of OmpF, an abundant outer membrane protein. Several recent papers have now shown that this is not an isolated case. At least five other sRNAs also regulate the synthesis of outer membrane ...
journal_title：Genes & development
pub_type： 杂志文章,评审
doi：10.1101/gad.1457506
更新日期：2006-09-01 00:00:00
abstract：:Cell fates in the optic neuroepithelium are determined by the combinational action of homeotic transcription factors. One of these is Vax2, a homeodomain protein that ventralizes the vertebrate eye field by repressing transcription of the Pax6 gene. We find that Vax2 shuttles between the nucleus and cytoplasm as a fun...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.1462706
更新日期：2006-10-15 00:00:00
abstract：:The mammalian high mobility group proteins HMG1 and HMG2 are abundant, chromatin-associated proteins whose cellular function is not known. In this study we show that these proteins can substitute for the prokaryotic DNA-bending protein HU in promoting the assembly of the Hin invertasome, an intermediate structure in H...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.7.8.1521
更新日期：1993-08-01 00:00:00
abstract：:The p53 tumor suppressor protein is the most well studied as a regulator of transcription in the nucleus, where it exists primarily as a tetramer. However, there are other oligomeric states of p53 that are relevant to its regulation and activities. In unstressed cells, p53 is normally held in check by MDM2 that target...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.304071.117
更新日期：2018-03-01 00:00:00
abstract：:The recombination site dif is the target on the Escherichia coli chromosome of the site-specific recombinases XerC and XerD. The dif/XerC-D system plays a role during the cell cycle, probably by favoring sister chromosome monomerization or separation. A phenomenon of regional control over dif activity, also analyzed i...
journal_title：Genes & development
pub_type： 评论,杂志文章
doi：10.1101/gad.10.9.1152
更新日期：1996-05-01 00:00:00
abstract：:The empty spiracles (ems) homeo box gene is required for the development of the Drosophila larval filzkörper, which are structural specializations of the eighth abdominal segment. Filzkörper development is also dependent on the function of the homeotic selector gene Abdominal-B (Abd-B). Here, we show that ems is a dow...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.7.2.229
更新日期：1993-02-01 00:00:00
abstract：:Faithful duplication of the genome relies on the ability to cope with an imperfect template. We investigated replication of UV-damaged DNA in Escherichia coli and found that ongoing replication stops for at least 15-20 min before resuming. Undamaged origins of replication (oriC) continue to fire at the normal rate and...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.417607
更新日期：2007-03-15 00:00:00
abstract：:The widespread class of RNA viruses that utilize internal ribosome entry sites (IRESs) for translation include poliovirus and Hepatitis C virus. To identify host factors required for IRES-dependent translation and viral replication, we performed a genome-wide RNAi screen in Drosophila cells infected with Drosophila C ...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.1267905
更新日期：2005-02-15 00:00:00
abstract：:Embryonic stem (ES) cells are pluripotent cells with the ability to self-renew indefinitely. These unique properties are controlled by genetic factors and chromatin structure. The exit from the self-renewing state is accompanied by changes in epigenetic chromatin modifications such as an induction in the silencing-ass...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.1588207
更新日期：2007-10-15 00:00:00
abstract：:Transabdominal (Tab), a dominant mutation in the Bithorax Complex (BX-C) of Drosophila, creates a sexually dimorphic pattern of segmental transformation that has complete penetrance and expressivity. Specific regions within the notum of the second thoracic segment (T2) are transformed into abdominal-like cuticle; thus...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.1.2.111
更新日期：1987-04-01 00:00:00
abstract：:Eukaryotic translation initiation begins with ribosomal recruitment of aminoacylated initiator tRNA (Met-tRNA(Met)(i)) by eukaryotic initiation factor eIF2. In cooperation with eIF3, eIF1, and eIF1A, Met-tRNA(Met)(i)/eIF2/GTP binds to 40S subunits yielding 43S preinitiation complexes that attach to the 5'-terminal reg...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.1957510
更新日期：2010-08-15 00:00:00
abstract：:Lipids play crucial roles in many aspects of glial cell biology, affecting processes ranging from myelin membrane biosynthesis to axo-glial interactions. In order to study the role of lipid metabolism in myelinating glial cells, we specifically deleted in Schwann cells the Lpin1 gene, which encodes the Mg2+-dependent ...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.1638008
更新日期：2008-06-15 00:00:00
abstract：:Plant water homeostasis is maintained by the phytohormone abscisic acid (ABA), which triggers stomatal pore closure in response to drought stress. We identified the Arabidopsis small guanosine triphosphatase (GTPase) protein AtRac1 as a central component in the ABA-mediated stomatal closure process. ABA treatment indu...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.900401
更新日期：2001-07-15 00:00:00
abstract：:Early endoderm formation in zebrafish requires at least three loci that function downstream of Nodal signaling but upstream of the early endodermal marker sox17: bonnie and clyde (bon), faust (fau), and casanova (cas). cas mutants show the most severe phenotype as they do not form any gut tissue and lack all sox17 exp...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.892301
更新日期：2001-06-15 00:00:00
abstract：:The contact site A glycoprotein is a developmentally regulated cell-surface component expressed during the aggregation stage of Dictyostelium discoideum. This protein has been implicated in the EDTA-stable (Ca2(+)-independent) type of cell adhesion of aggregating cells. The gene coding for the contact site A protein w...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.3.12a.2011
更新日期：1989-12-01 00:00:00
abstract：:Ssn6-Tup1 regulates multiple genes in yeast, providing a paradigm for corepressor functions. Tup1 interacts directly with histones H3 and H4, and mutation of these histones synergistically compromises Ssn6-Tup1-mediated repression. In vitro, Tup1 interacts preferentially with underacetylated isoforms of H3 and H4, sug...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.829100
更新日期：2000-11-01 00:00:00
abstract：:Developmental responses to the plant hormone auxin are thought to be mediated by interacting pairs from two protein families: short-lived inhibitory IAA proteins and ARF transcription factors binding to auxin-response elements. monopteros mutants lacking activating ARF5 and the auxin-insensitive mutant bodenlos fail t...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.229402
更新日期：2002-07-01 00:00:00
abstract：:In response to skeletal muscle injury, satellite cells, which function as a myogenic stem cell population, become activated, expand through proliferation, and ultimately fuse with each other and with damaged myofibers to promote muscle regeneration. Here, we show that members of the Myocardin family of transcriptional...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.179663.111
更新日期：2012-01-15 00:00:00
abstract：:The RNA polymerase II core promoter is a structurally and functionally diverse transcriptional module. RNAi depletion and overexpression experiments revealed a genetic circuit that controls the balance of transcription from two core promoter motifs, the TATA box and the downstream core promoter element (DPE). In this ...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.1681808
更新日期：2008-09-01 00:00:00
abstract：:The Notch signaling pathway is essential for embryonic vascular development in vertebrates. Here we show that mouse embryos heterozygous for a targeted mutation in the gene encoding the DLL4 ligand exhibit haploinsufficient lethality because of defects in vascular remodeling. We also describe vascular defects in embry...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.1239204
更新日期：2004-10-15 00:00:00
abstract：:In a four-cell-stage Caenorhabditis elegans embryo, Wnt signaling polarizes an endoderm precursor called EMS. The polarization of this cell orients its mitotic spindle in addition to inducing endodermal fate in one daughter cell. Reducing the function of Wnt pathway genes, including a newly identified GSK-3beta homolo...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.13.15.2028
更新日期：1999-08-01 00:00:00
abstract：:U4 and U6 small nuclear RNAs are associated by an extensive base-pairing interaction that must be disrupted and reformed with each round of splicing. U4 mutations within the U4/U6 interaction domain destabilize the complex in vitro and cause a cold-sensitive phenotype in vivo. Restabilization of the U4/U6 helix by dom...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.5.5.773
更新日期：1991-05-01 00:00:00
abstract：:The LIM motif defines a double zinc finger structure found in proteins involved in cell fate determination and growth control. LIM proteins, which include LIM homeo domain, LIM kinase, focal adhesion, and LIM-only proteins, usually contain two or more LIM motifs clustered at their amino- or carboxy-terminal end. At pr...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.10.3.289
更新日期：1996-02-01 00:00:00
abstract：:Recruitment of p300/CBP by the hypoxia-inducible factor, HIF-1, is essential for the transcriptional response to hypoxia and requires an interaction between the p300/CBP CH1 region and HIF-1alpha. A new p300-CH1 interacting protein, p35srj, has been identified and cloned. p35srj is an alternatively spliced isoform of ...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.13.1.64
更新日期：1999-01-01 00:00:00
abstract：:Cellular senescence limits proliferation of potentially detrimental cells, preventing tumorigenesis and restricting tissue damage. However, the function of senescence in nonpathological conditions is unknown. We found that the human placental syncytiotrophoblast exhibited the phenotype and expressed molecular markers ...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.227512.113
更新日期：2013-11-01 00:00:00
