Abstract：
:One of the most common microsatellites in eukaryotes consists of tandem arrays of the dinucleotide GT. Although the study of the instability of such repetitive DNA has been extremely fruitful over the last decade, no biological function has been demonstrated for these sequences. We investigated the genetic behavior of a region of the yeast Saccharomyces cerevisiae genome containing a 39-CA/GT dinucleotide repeat sequence. When the microsatellite sequence was present at the ARG4 locus on homologous chromosomes, diploid cells undergoing meiosis generated an excess of tetrads containing a conversion of the region restricted to the region of the microsatellite close to the recombination-initiation double-strand break. Moreover, whereas the repetitive sequence had no effect on the frequency of single crossover, its presence strongly stimulated the formation of multiple crossovers. The combined data strongly suggest that numerous recombination events are restricted to the initiation side of the microsatellite as though progression of the strand exchange initiated at the ARG4 promoter locus was impaired by the repetitive sequence. This observation corroborates in vitro experiments that demonstrated that RecA-promoted strand exchange is inhibited by CA/GT dinucleotide tracts. Surprisingly, meiotic instability of the microsatellite was very high (>0.1 alterations per tetrad) in all the spores with parental and recombinant chromosomes.
journal_name
Genes Devjournal_title
Genes & developmentauthors
Gendrel CG,Boulet A,Dutreix Msubject
Has Abstractpub_date
2000-05-15 00:00:00pages
1261-8issue
10eissn
0890-9369issn
1549-5477journal_volume
14pub_type
杂志文章abstract：:Transcription by RNA polymerase II (RNAPII) is a dynamic process with frequent variations in the elongation rate. However, the physiological relevance of variations in RNAPII elongation kinetics has remained unclear. Here we show in yeast that a RNAPII mutant that reduces the transcription elongation rate causes wides...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.337212.120
更新日期：2020-07-01 00:00:00
abstract：:The amyloid precursor protein (APP) has garnered considerable attention due to its genetic links to Alzheimer's disease. Death receptor 6 (DR6) was recently shown to bind APP via the protein extracellular regions, stimulate axonal pruning, and inhibit synapse formation. Here, we report the crystal structure of the DR6...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.257675.114
更新日期：2015-04-15 00:00:00
abstract：:Small cell lung cancer (SCLC) is an aggressive neuroendocrine cancer characterized by initial chemosensitivity followed by emergence of chemoresistant disease. To study roles for MYCN amplification in SCLC progression and chemoresistance, we developed a genetically engineered mouse model of MYCN-overexpressing SCLC. I...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.340133.120
更新日期：2020-09-01 00:00:00
abstract：:Chromosomal processes related to formation and function of meiotic chiasmata have been analyzed in Sordaria macrospora. Double-strand breaks (DSBs), programmed or gamma-rays-induced, are found to promote four major events beyond recombination and accompanying synaptonemal complex formation: (1) juxtaposition of homolo...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.275203
更新日期：2003-11-01 00:00:00
abstract：:In this issue of Genes & Development, Wierzbicki and colleagues (pp. 1825-1836) examine the current model of RNA-directed DNA methylation (RdDM) by determining genome-wide distributions of RNA polymerase V (Pol V) occupancy, siRNAs, and DNA methylation. Their data support the key role of base-pairing between Pol V tra...
journal_title：Genes & development
pub_type： 评论,杂志文章
doi：10.1101/gad.200410.112
更新日期：2012-08-15 00:00:00
abstract：:The physiological functions of many vital tissues and organs continue to mature after birth, but the genetic mechanisms governing this postnatal maturation remain an unsolved mystery. Human pancreatic β cells produce and secrete insulin in response to physiological cues like glucose, and these hallmark functions impro...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.342378.120
更新日期：2021-01-14 00:00:00
abstract：:Eph receptors and ephrin ligands are key players in many developmental processes including embryo patterning, angiogenesis, and axon guidance. Eph/ephrin interactions lead to the generation of a bidirectional signal, in which both the Eph receptors and the ephrins activate downstream signaling cascades simultaneously....
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.1171704
更新日期：2004-03-01 00:00:00
abstract：:eIF2B is a heteropentameric guanine-nucleotide exchange factor essential for protein synthesis initiation in eukaryotes. Its activity is inhibited in response to starvation or stress by phosphorylation of the alpha subunit of its substrate, translation initiation factor eIF2, resulting in reduced rates of translation ...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.12.4.514
更新日期：1998-02-15 00:00:00
abstract：:The sigma(28) protein is a member of the bacterial sigma(70)-family of transcription factors that directs RNA polymerase to flagellar late (class 3) promoters. The sigma(28) protein is regulated in response to flagellar assembly by the anti-sigma(28) factor FlgM. FlgM inhibits sigma(28)-dependent transcription of gene...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.380406
更新日期：2006-08-15 00:00:00
abstract：:During embryonic development in vertebrates, the endoderm becomes patterned along the anteroposterior axis to produce distinct derivatives. How this regulation is controlled is not well understood. We report that the zebrafish hairy/enhancer of split [E(spl)]-related gene her5 plays a critical role in this process. At...
journal_title：Genes & development
pub_type： 杂志文章
doi：
更新日期：2000-07-01 00:00:00
abstract：:Oligophrenin-1 (OPHN1) encodes a Rho-GTPase-activating protein (Rho-GAP) whose loss of function has been associated with X-linked mental retardation (MR). The pathophysiological role of OPHN1, however, remains poorly understood. Here we show that OPHN1 through its Rho-GAP activity plays a critical role in the activity...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.1783809
更新日期：2009-06-01 00:00:00
abstract：:One of the first steps in neurogenesis is the diversification of cells along the dorsoventral axis. In Drosophila the central nervous system develops from three longitudinal columns of cells: ventral cells that express the vnd/nk2 homeobox gene, intermediate cells, and dorsal cells that express the msh homeobox gene. ...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.12.22.3591
更新日期：1998-11-15 00:00:00
abstract：:Placental development is profoundly influenced by oxygen (O(2)) tension. Human cytotrophoblasts proliferate in vitro under low O(2) conditions but differentiate at higher O(2) levels, mimicking the developmental transition they undergo as they invade the placental bed to establish the maternal-fetal circulation in viv...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.853700
更新日期：2000-12-15 00:00:00
abstract：:The generation of neurons by progenitor cells involves the tight coordination of multiple cellular activities, including cell cycle exit, initiation of neuronal differentiation, and cell migration. The mechanisms that integrate these different events into a coherent developmental program are not well understood. Here ...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.377106
更新日期：2006-06-01 00:00:00
abstract：:Cis-regulatory modules (CRMs) are defined by unique combinations of transcription factor-binding sites. Emerging evidence suggests that the number, affinity, and organization of sites play important roles in regulating enhancer output and, ultimately, gene expression. Here, we investigate how the cis-regulatory logic ...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.307132.117
更新日期：2018-03-01 00:00:00
abstract：:AMP-activated protein kinase (AMPK) is a sensor of energy status that maintains cellular energy homeostasis. It arose very early during eukaryotic evolution, and its ancestral role may have been in the response to starvation. Recent work shows that the kinase is activated by increases not only in AMP, but also in ADP....
journal_title：Genes & development
pub_type： 杂志文章,评审
doi：10.1101/gad.17420111
更新日期：2011-09-15 00:00:00
abstract：:The hair follicle undergoes a lifelong developmental cycle that depends on the integration between activating and inhibitory signals acting to regulate and guide the proliferation and differentiation of pluripotent epithelial stem cells. The effectors and mechanisms responsible for re-entry of quiescent telogen hair f...
journal_title：Genes & development
pub_type： 评论,杂志文章
doi：10.1101/gad.223172.113
更新日期：2013-06-15 00:00:00
abstract：:The association of proteins with the branch site region during pre-mRNA splicing was probed using a novel methodology to site-specifically modify the pre-mRNA with the photo-reagent benzophenone. Three sets of proteins were distinguished by the kinetics of their associations with pre-mRNAs, by their association with d...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.8.24.3008
更新日期：1994-12-15 00:00:00
abstract：:GRF2, an abundant yeast protein of Mr approximately 127,000, binds to the GAL upstream activating sequence (UASG) and creates a nucleosome-free region of approximately 230 bp. Purified GRF2 binds to sequences found in many other UASs, in the 35S rRNA enhancer, at centromeres, and at telomeres. Although GRF2 stimulates...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.4.4.503
更新日期：1990-04-01 00:00:00
abstract：:The number of known long noncoding RNA (lncRNA) functions is rapidly growing, but how those functions are encoded in their sequence and structure remains poorly understood. NORAD (noncoding RNA activated by DNA damage) is a recently characterized, abundant, and highly conserved lncRNA that is required for proper mitot...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.309138.117
更新日期：2018-01-01 00:00:00
abstract：:The recombinational enhancer of the Hin inversion system in Salmonella stimulates recombination in vitro 150-fold in the presence of the Escherichia coli host factor Fis. To gain an understanding of the roles of the enhancer and Fis in stimulating the Hin-mediated inversion reaction, we have used nuclease and chemical...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.1.8.762
更新日期：1987-10-01 00:00:00
abstract：:Chromatin reorganization is essential for transcriptional control by sequence-specific transcription factors. However, the molecular link between transcriptional control and chromatin reconfiguration remains unclear. By colocalization of the nuclear ecdysone receptor (EcR) on the ecdysone-induced puff in the salivary ...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.1857410
更新日期：2010-01-15 00:00:00
abstract：:The labial (lab) gene of Drosophila melanogaster is necessary for the proper development of the embryonic (larval) and adult head. We have identified the lab transcription unit within the proximal portion of the Antennapedia Complex (ANT-C) by mapping the molecular lesions associated with chromosomally rearranged lab ...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.3.3.399
更新日期：1989-03-01 00:00:00
abstract：:Genetic screening and selection procedures employing a secA-lacZ fusion strain repeatedly have yielded mutations in four genes affecting the protein export pathway of Escherichia coli. These genes are secA, secD, prlA/secY, and secE. We discuss the significance of the failure to find new sec genes after extensive use ...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.3.7.1035
更新日期：1989-07-01 00:00:00
abstract：:Calcineurin, a conserved Ca(2+)/calmodulin-regulated protein phosphatase, plays a crucial role in Ca(2+) signaling in a wide variety of cell types. In Saccharomyces cerevisiae, calcineurin positively regulates transcription in response to stress by dephosphorylating the transcription factor Crz1p/Tcn1p. Dephosphorylat...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.967602
更新日期：2002-03-01 00:00:00
abstract：:Myxococcus xanthus fruiting body development is induced by amino acid limitation. The decision to grow or develop is established by the RelA-dependent stringent response and A-signaling. We identified two new members of this regulatory hierarchy, socE and the C-signaling gene csgA. SocE depletion arrests growth and in...
journal_title：Genes & development
pub_type： 杂志文章
doi：
更新日期：2000-02-15 00:00:00
abstract：:Hepatocyte nuclear factor 1 (HNF-1) is a transcriptional regulatory protein possibly involved in the activation of many liver-specifically expressed genes. HNF-1 mRNA is restricted to a small number of tissues, suggesting that the HNF-1 gene itself is regulated at the transcriptional level. We have isolated and charac...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.5.12a.2225
更新日期：1991-12-01 00:00:00
abstract：:Although branchpoint recognition is an essential component of intron excision during the RNA splicing process, the branchpoint itself is frequently assumed to be a basal, rather than regulatory, sequence feature. However, this assumption has not been systematically tested due to the technical difficulty of identifying...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.312058.118
更新日期：2018-04-01 00:00:00
abstract：:Transcriptional activation of the prolactin and growth hormone genes, occurring in a cell-specific fashion, requires short-range synergistic interactions between the pituitary-specific POU domain factor Pit-1 and other transcription factors, particularly nuclear receptors. Unexpectedly, we find that these events invol...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.9.16.1992
更新日期：1995-08-15 00:00:00
abstract：:Two recent reports, including one by Reinders and colleagues (pp. 939-950) in the April 15, 2009, issue of Genes & Development, describe the construction of Arabidopsis recombinant inbred populations that maximize epigenetic rather than genetic variation. The distribution and behavior of phenotypic variation in these ...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.1824909
更新日期：2009-07-15 00:00:00
