Abstract:
:The contact site A glycoprotein is a developmentally regulated cell-surface component expressed during the aggregation stage of Dictyostelium discoideum. This protein has been implicated in the EDTA-stable (Ca2(+)-independent) type of cell adhesion of aggregating cells. The gene coding for the contact site A protein was disrupted by homologous recombination, using a transformation vector that contained a 1.0-kb cDNA fragment as an insert. Transformants that did not express the protein were identified by colony immunoblotting. These transformants produced three truncated contact site A transcripts. One of them was controlled by the original contact site A promoter, as indicated by its strict developmental regulation and cAMP inducibility; the other two transcripts were transcribed from the actin 6 promoter of the vector. When cell adhesion was assayed in the transformants by agitating suspended cells in an agglutinometer, EDTA-stable adhesion was drastically reduced as compared to wild type, confirming that the contact site A glycoprotein acts as a cell-adhesion molecule. However, aggregation of the transformed cells on an agar surface was not remarkably altered. These results suggest that the contact site A glycoprotein is responsible for a 'fast' type of cell adhesion that is essential when aggregating cells are subjected to shear. When cells are not mechanically disturbed, a 'slow' type of adhesion mediated by other molecules is sufficient for their aggregation.
journal_name
Genes Devjournal_title
Genes & developmentauthors
Harloff C,Gerisch G,Noegel AAdoi
10.1101/gad.3.12a.2011subject
Has Abstractpub_date
1989-12-01 00:00:00pages
2011-9issue
12Aeissn
0890-9369issn
1549-5477journal_volume
3pub_type
杂志文章abstract::The Rbfox proteins (Rbfox1, Rbfox2, and Rbfox3) regulate the alternative splicing of many important neuronal transcripts and have been implicated in a variety of neurological disorders. However, their roles in brain development and function are not well understood, in part due to redundancy in their activities. Here w...
journal_title:Genes & development
pub_type: 杂志文章
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journal_title:Genes & development
pub_type: 杂志文章
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journal_title:Genes & development
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journal_title:Genes & development
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journal_title:Genes & development
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journal_title:Genes & development
pub_type: 杂志文章
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更新日期:2006-04-01 00:00:00
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journal_title:Genes & development
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journal_title:Genes & development
pub_type: 评论,杂志文章
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journal_title:Genes & development
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更新日期:2006-06-01 00:00:00
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journal_title:Genes & development
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journal_title:Genes & development
pub_type: 杂志文章
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更新日期:1998-07-01 00:00:00
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journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.10.6.740
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journal_title:Genes & development
pub_type: 杂志文章
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更新日期:2002-08-01 00:00:00
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journal_title:Genes & development
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journal_title:Genes & development
pub_type: 杂志文章
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更新日期:2008-02-01 00:00:00
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journal_title:Genes & development
pub_type: 杂志文章
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journal_title:Genes & development
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journal_title:Genes & development
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journal_title:Genes & development
pub_type: 杂志文章
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