A unique zinc finger protein is associated preferentially with active ecdysone-responsive loci in Drosophila.


:Using an immunochemical approach, we have identified a unique antigen, PEP (protein on ecdysone puffs), which is associated in third-instar larvae with the active ecdysone-regulated loci on polytene chromosomes; PEP is not associated with most intermolt puffs and is found on some, but not all, heat shock-induced puffs. The distribution pattern changes with changing puffing patterns in the developmental program. We have screened an expression library and recovered a cDNA clone encoding PEP. PEP possesses multiple potential nucleic acid- and protein- binding regions: a glycine- and asparagine-rich amino terminus, four zinc finger motifs, two very acidic segments, two short basic stretches, and an alanine- and proline-rich carboxyl terminus. The Pep gene maps by in situ hybridization to the cytological locus 74F, adjacent to the early ecdysone-responsive region; however, the gene is not regulated by ecdysone at the level of transcription. The pattern of Pep expression through development suggests that maternal Pep gene transcripts are supplied to the embryo, and that the abundance of Pep gene transcripts decreases to a lower, fairly constant level thereafter. This unusual protein may play a role in the process of gene activation, or possibly in RNA processing, for a defined set of developmentally regulated loci.


Genes Dev


Genes & development


Amero SA,Elgin SC,Beyer AL




Has Abstract


1991-02-01 00:00:00












  • Phase separation of Polycomb-repressive complex 1 is governed by a charged disordered region of CBX2.

    abstract::Mammalian development requires effective mechanisms to repress genes whose expression would generate inappropriately specified cells. The Polycomb-repressive complex 1 (PRC1) family complexes are central to maintaining this repression. These include a set of canonical PRC1 complexes, each of which contains four core p...

    journal_title:Genes & development

    pub_type: 杂志文章


    authors: Plys AJ,Davis CP,Kim J,Rizki G,Keenen MM,Marr SK,Kingston RE

    更新日期:2019-07-01 00:00:00

  • Expression patterns of the homeo box-containing genes En-1 and En-2 and the proto-oncogene int-1 diverge during mouse development.

    abstract::We have compared the expression of the murine genes En-1,En-2, and in-1 during development by in situ hybridization. Expression of all three genes was first detected at 8.0 days in overlapping bands of the anterior neural folds. By 12.0 days the expression patterns diverged. En-1 and En-2 were expressed in a similar r...

    journal_title:Genes & development

    pub_type: 杂志文章


    authors: Davis CA,Joyner AL

    更新日期:1988-12-01 00:00:00

  • Isolation, structure, and expression of labial, a homeotic gene of the Antennapedia Complex involved in Drosophila head development.

    abstract::The labial (lab) gene of Drosophila melanogaster is necessary for the proper development of the embryonic (larval) and adult head. We have identified the lab transcription unit within the proximal portion of the Antennapedia Complex (ANT-C) by mapping the molecular lesions associated with chromosomally rearranged lab ...

    journal_title:Genes & development

    pub_type: 杂志文章


    authors: Diederich RJ,Merrill VK,Pultz MA,Kaufman TC

    更新日期:1989-03-01 00:00:00

  • Nitric oxide directly regulates gene expression during Drosophila development: need some gas to drive into metamorphosis?

    abstract::Nitric oxide (NO) is an important second messenger involved in numerous biological processes, but how it regulates gene expression is not well understood. In this issue of Genes & Development, Cáceres and colleagues (pp. 1476-1485) report a critical requirement of NO as a direct regulator of gene expression through it...

    journal_title:Genes & development

    pub_type: 评论,杂志文章


    authors: Yamanaka N,O'Connor MB

    更新日期:2011-07-15 00:00:00

  • Against the oxidative damage theory of aging: superoxide dismutases protect against oxidative stress but have little or no effect on life span in Caenorhabditis elegans.

    abstract::The superoxide radical (O(2)(-)) has long been considered a major cause of aging. O(2)(-) in cytosolic, extracellular, and mitochondrial pools is detoxified by dedicated superoxide dismutase (SOD) isoforms. We tested the impact of each SOD isoform in Caenorhabditis elegans by manipulating its five sod genes and saw no...

    journal_title:Genes & development

    pub_type: 杂志文章


    authors: Doonan R,McElwee JJ,Matthijssens F,Walker GA,Houthoofd K,Back P,Matscheski A,Vanfleteren JR,Gems D

    更新日期:2008-12-01 00:00:00

  • Genomic imprinting in plants-revisiting existing models.

    abstract::Genomic imprinting is an epigenetic phenomenon leading to parentally biased gene expression. Throughout the years, extensive efforts have been made to characterize the epigenetic marks underlying imprinting in animals and plants. As a result, DNA methylation asymmetries between parental genomes emerged as the primary ...

    journal_title:Genes & development

    pub_type: 杂志文章,评审


    authors: Batista RA,Köhler C

    更新日期:2020-01-01 00:00:00

  • Histone chaperone ASF1 cooperates with the Brahma chromatin-remodelling machinery.

    abstract::De novo chromatin assembly into regularly spaced nucleosomal arrays is essential for eukaryotic genome maintenance and inheritance. The Anti-Silencing Function 1 protein (ASF1) has been shown to be a histone chaperone, participating in DNA-replication-coupled nucleosome assembly. We show that mutations in the Drosophi...

    journal_title:Genes & development

    pub_type: 杂志文章


    authors: Moshkin YM,Armstrong JA,Maeda RK,Tamkun JW,Verrijzer P,Kennison JA,Karch F

    更新日期:2002-10-15 00:00:00

  • The SABRE gene is required for normal cell expansion in Arabidopsis.

    abstract::Regulated cell expansion is an important determinant of organ shape in higher plants. The sabre mutation results in abnormal cell expansion in Arabidopsis. There is a shift in the orientation of expansion evident primarily in root cortex cells. The SABRE gene has been cloned and found to encode a novel protein. Reduct...

    journal_title:Genes & development

    pub_type: 杂志文章


    authors: Aeschbacher RA,Hauser MT,Feldmann KA,Benfey PN

    更新日期:1995-02-01 00:00:00

  • The role of a Williams-Beuren syndrome-associated helix-loop-helix domain-containing transcription factor in activin/nodal signaling.

    abstract::We investigated the regulation of the activin/nodal-inducible distal element (DE) of the Xenopus goosecoid (gsc) promoter. On the basis of its interaction with the DE, we isolated a Xenopus homolog of the human Williams-Beuren syndrome critical region 11 (XWBSCR11), and further, show that it interacts with pathway-spe...

    journal_title:Genes & development

    pub_type: 杂志文章


    authors: Ring C,Ogata S,Meek L,Song J,Ohta T,Miyazono K,Cho KW

    更新日期:2002-04-01 00:00:00

  • The Stat6-regulated KRAB domain zinc finger protein Zfp157 regulates the balance of lineages in mammary glands and compensates for loss of Gata-3.

    abstract::Lineage commitment studies in mammary glands have focused on identifying cell populations that display stem or progenitor properties. However, the mechanisms that control cell fate have been incompletely explored. Herein we show that zinc finger protein 157 (Zfp157) is required to establish the balance between luminal...

    journal_title:Genes & development

    pub_type: 杂志文章


    authors: Oliver CH,Khaled WT,Frend H,Nichols J,Watson CJ

    更新日期:2012-05-15 00:00:00

  • A genetic screen identifies the Triple T complex required for DNA damage signaling and ATM and ATR stability.

    abstract::In response to DNA damage, cells activate a complex signal transduction network called the DNA damage response (DDR). To enhance our current understanding of the DDR network, we performed a genome-wide RNAi screen to identify genes required for resistance to ionizing radiation (IR). Along with a number of known DDR ge...

    journal_title:Genes & development

    pub_type: 杂志文章


    authors: Hurov KE,Cotta-Ramusino C,Elledge SJ

    更新日期:2010-09-01 00:00:00

  • A variant limb deformity transcript expressed in the embryonic mouse limb defines a novel formin.

    abstract::The formins constitute a set of protein isoforms encoded by the alternatively spliced transcripts arising from the limb deformity (ld) locus of the mouse. Mutations in this locus disrupt formation of the anteroposterior axis of the embryonic limb. Although ld transcripts are widely expressed during embryogenesis, we h...

    journal_title:Genes & development

    pub_type: 杂志文章


    authors: Jackson-Grusby L,Kuo A,Leder P

    更新日期:1992-01-01 00:00:00

  • H3.3 actively marks enhancers and primes gene transcription via opening higher-ordered chromatin.

    abstract::The histone variants H3.3 and H2A.Z have recently emerged as two of the most important features in transcriptional regulation, the molecular mechanism of which still remains poorly understood. In this study, we investigated the regulation of H3.3 and H2A.Z on chromatin dynamics during transcriptional activation. Our i...

    journal_title:Genes & development

    pub_type: 杂志文章


    authors: Chen P,Zhao J,Wang Y,Wang M,Long H,Liang D,Huang L,Wen Z,Li W,Li X,Feng H,Zhao H,Zhu P,Li M,Wang QF,Li G

    更新日期:2013-10-01 00:00:00

  • Chromatin remodeler CHD7 regulates the stem cell identity of human neural progenitors.

    abstract::Multiple congenital disorders often present complex phenotypes, but how the mutation of individual genetic factors can lead to multiple defects remains poorly understood. In the present study, we used human neuroepithelial (NE) cells and CHARGE patient-derived cells as an in vitro model system to identify the function...

    journal_title:Genes & development

    pub_type: 杂志文章


    authors: Chai M,Sanosaka T,Okuno H,Zhou Z,Koya I,Banno S,Andoh-Noda T,Tabata Y,Shimamura R,Hayashi T,Ebisawa M,Sasagawa Y,Nikaido I,Okano H,Kohyama J

    更新日期:2018-01-15 00:00:00

  • Integrative genome analysis of somatic p53 mutant osteosarcomas identifies Ets2-dependent regulation of small nucleolar RNAs by mutant p53 protein.

    abstract::TP53 is the most frequently mutated gene in human cancer. Many mutant p53 proteins exert oncogenic gain-of-function (GOF) properties that contribute to metastasis, but the mechanisms mediating these functions remain poorly defined in vivo. To elucidate how mutant p53 GOF drives metastasis, we developed a traceable som...

    journal_title:Genes & development

    pub_type: 杂志文章


    authors: Pourebrahim R,Zhang Y,Liu B,Gao R,Xiong S,Lin PP,McArthur MJ,Ostrowski MC,Lozano G

    更新日期:2017-09-15 00:00:00

  • The TIR1 protein of Arabidopsis functions in auxin response and is related to human SKP2 and yeast grr1p.

    abstract::Genetic analysis in Arabidopsis has led to the identification of several genes that are required for auxin response. One of these genes, AXR1, encodes a protein related to yeast Aos1p, a protein that functions to activate the ubiquitin-related protein Smt3p. Here we report the identification of a new gene called TRANS...

    journal_title:Genes & development

    pub_type: 杂志文章


    authors: Ruegger M,Dewey E,Gray WM,Hobbie L,Turner J,Estelle M

    更新日期:1998-01-15 00:00:00

  • Differentiation-induced gene expression in 3T3-L1 preadipocytes: CCAAT/enhancer binding protein interacts with and activates the promoters of two adipocyte-specific genes.

    abstract::Previous studies have shown that differentiation of 3T3-L1 preadipocytes leads to the transcriptional activation of a group of adipose-specific genes. As an approach to defining the mechanism responsible for activating the expression of these genes, we investigated the binding of nuclear factors to the promoters of tw...

    journal_title:Genes & development

    pub_type: 杂志文章


    authors: Christy RJ,Yang VW,Ntambi JM,Geiman DE,Landschulz WH,Friedman AD,Nakabeppu Y,Kelly TJ,Lane MD

    更新日期:1989-09-01 00:00:00

  • A model for the abrogation of the SOS response by an SOS protein: a negatively charged helix in DinI mimics DNA in its interaction with RecA.

    abstract::DinI is a recently described negative regulator of the SOS response in Escherichia coli. Here we show that it physically interacts with RecA and prevents the binding of single-stranded DNA to RecA, which is required for the activation of the latter. DinI also displaces ssDNA from a stable RecA-DNA cofilament, thus eli...

    journal_title:Genes & development

    pub_type: 杂志文章


    authors: Voloshin ON,Ramirez BE,Bax A,Camerini-Otero RD

    更新日期:2001-02-15 00:00:00

  • Novel role for a bacterial nucleoid protein in translation of mRNAs with suboptimal ribosome-binding sites.

    abstract::In Escherichia coli, the major nucleoid protein H-NS limits transcription by acting as a repressor or transcriptional silencer, presumably by its ability to close the looped chromosome domains in the nucleoid through DNA-protein-DNA bridging. Here, we demonstrate the direct involvement of H-NS as a positive factor sti...

    journal_title:Genes & development

    pub_type: 杂志文章


    authors: Park HS,Ostberg Y,Johansson J,Wagner EG,Uhlin BE

    更新日期:2010-07-01 00:00:00

  • Dynamic SpoIIIE assembly mediates septal membrane fission during Bacillus subtilis sporulation.

    abstract::SpoIIIE is an FtsK-related protein that transports the forespore chromosome across the Bacillus subtilis sporulation septum. We use membrane photobleaching and protoplast assays to demonstrate that SpoIIIE is required for septal membrane fission in the presence of trapped DNA, and that DNA is transported across separa...

    journal_title:Genes & development

    pub_type: 杂志文章


    authors: Fleming TC,Shin JY,Lee SH,Becker E,Huang KC,Bustamante C,Pogliano K

    更新日期:2010-06-01 00:00:00

  • Half helical turn spacing changes convert a frog into a mouse rDNA promoter: a distant upstream domain determines the helix face of the initiation site.

    abstract::Transcription of frog rDNA by mouse cell factors is the only documented exception to the observed species selectivity of rRNA gene expression. This heterologous transcription is authentic in that it uses the normal frog upstream and core promoter domains, as well as the normal mouse polymerase I transcription factors,...

    journal_title:Genes & development

    pub_type: 杂志文章


    authors: Pape LK,Windle JJ,Sollner-Webb B

    更新日期:1990-01-01 00:00:00

  • Developmental control of the G1 to S transition in Drosophila: cyclin Eis a limiting downstream target of E2F.

    abstract::The E2F transcription factor is required for S phase in Drosophila. While it also triggers expression of replication genes at the G1-S transition, the relevance of this transcription is not clear because many of the induced gene products are sufficiently stable that new expression is not required for S phase. However,...

    journal_title:Genes & development

    pub_type: 杂志文章


    authors: Duronio RJ,O'Farrell PH

    更新日期:1995-06-15 00:00:00

  • Regulation of plant innate immunity by three proteins in a complex conserved across the plant and animal kingdoms.

    abstract::Innate immunity against pathogen infection is an evolutionarily conserved process among multicellular organisms. Arabidopsis SNC1 encodes a Resistance protein that combines attributes of multiple mammalian pattern recognition receptors. Utilizing snc1 as an autoimmune model, we identified a discrete protein complex co...

    journal_title:Genes & development

    pub_type: 杂志文章


    authors: Palma K,Zhao Q,Cheng YT,Bi D,Monaghan J,Cheng W,Zhang Y,Li X

    更新日期:2007-06-15 00:00:00

  • SMG6 interacts with the exon junction complex via two conserved EJC-binding motifs (EBMs) required for nonsense-mediated mRNA decay.

    abstract::Nonsense-mediated mRNA decay (NMD) is a quality control mechanism that detects and degrades mRNAs containing premature stop codons (PTCs). In vertebrates, PTCs trigger efficient NMD when located upstream of an exon junction complex (EJC). Degradation of PTC-containing mRNAs requires the endonucleolytic activity of SMG...

    journal_title:Genes & development

    pub_type: 杂志文章


    authors: Kashima I,Jonas S,Jayachandran U,Buchwald G,Conti E,Lupas AN,Izaurralde E

    更新日期:2010-11-01 00:00:00

  • Mouse models for human lung cancer.

    abstract::In recent years several new mouse models for lung cancer have been described. These include models for both non-small-cell lung cancer (NSCLC) and small-cell lung cancer (SCLC). Tumorigenesis in these conditional mouse tumor models can be initiated in adult mice through Cre-recombinase-induced activation of oncogenic ...

    journal_title:Genes & development

    pub_type: 杂志文章,评审


    authors: Meuwissen R,Berns A

    更新日期:2005-03-15 00:00:00

  • The global transcriptional regulators, SSN6 and TUP1, play distinct roles in the establishment of a repressive chromatin structure.

    abstract::Repression of a-cell specific gene expression in yeast alpha cells requires MAT alpha 2 and MCM1, as well as two global repressors, SSN6 and TUP1. Previous studies demonstrated that nucleosomes positioned adjacent to the alpha 2/MCM1 operator in alpha cells directly contribute to repression. To investigate the possibi...

    journal_title:Genes & development

    pub_type: 杂志文章


    authors: Cooper JP,Roth SY,Simpson RT

    更新日期:1994-06-15 00:00:00

  • Escherichia coli single-stranded DNA-binding protein is a supercoiled template-dependent transcriptional activator of N4 virion RNA polymerase.

    abstract::Coliphage N4 is a double-stranded DNA virus that requires the sequential activity of three different RNA polymerases during infection. The N4 virion RNA polymerase, which is carried in the virion and is injected with the DNA at the start of infection, is responsible for the synthesis of N4 early RNAs. In vitro, the vi...

    journal_title:Genes & development

    pub_type: 杂志文章


    authors: Markiewicz P,Malone C,Chase JW,Rothman-Denes LB

    更新日期:1992-10-01 00:00:00

  • ICP27 mediates HSV RNA export by shuttling through a leucine-rich nuclear export signal and binding viral intronless RNAs through an RGG motif.

    abstract::Infection of metazoan cells with some viruses alters the balance of cellular mRNA export to favor viral RNA export and to retain cellular transcripts in the nucleus. Here, evidence is presented to show that the herpes simplex virus 1 (HSV-1) essential regulatory protein ICP27, which inhibits host cell-splicing, result...

    journal_title:Genes & development

    pub_type: 杂志文章


    authors: Sandri-Goldin RM

    更新日期:1998-03-15 00:00:00

  • Checkpoint activation regulates mutagenic translesion synthesis.

    abstract::Cells have evolved checkpoint responses to arrest or delay the cell cycle, activate DNA repair networks, or induce apoptosis after genomic perturbation. Cells have also evolved the translesion synthesis processes to tolerate genomic lesions by either error-free or error-prone repair. Here, we show that after a replica...

    journal_title:Genes & development

    pub_type: 杂志文章


    authors: Kai M,Wang TS

    更新日期:2003-01-01 00:00:00

  • The LIM domain-containing homeo box gene Xlim-1 is expressed specifically in the organizer region of Xenopus gastrula embryos.

    abstract::A novel cysteine-rich motif, named LIM, has been identified in the homeo box genes lin-11, Isl-1, and mec-3; the mec-3 and lin-11 genes determine cell lineages in Caenorhabditis elegans. We isolated LIM class homeo box genes from Xenopus laevis that are closely related to lin-11 and mec-3 in the LIM and homeo domains....

    journal_title:Genes & development

    pub_type: 杂志文章


    authors: Taira M,Jamrich M,Good PJ,Dawid IB

    更新日期:1992-03-01 00:00:00