A unique zinc finger protein is associated preferentially with active ecdysone-responsive loci in Drosophila.

Abstract:

:Using an immunochemical approach, we have identified a unique antigen, PEP (protein on ecdysone puffs), which is associated in third-instar larvae with the active ecdysone-regulated loci on polytene chromosomes; PEP is not associated with most intermolt puffs and is found on some, but not all, heat shock-induced puffs. The distribution pattern changes with changing puffing patterns in the developmental program. We have screened an expression library and recovered a cDNA clone encoding PEP. PEP possesses multiple potential nucleic acid- and protein- binding regions: a glycine- and asparagine-rich amino terminus, four zinc finger motifs, two very acidic segments, two short basic stretches, and an alanine- and proline-rich carboxyl terminus. The Pep gene maps by in situ hybridization to the cytological locus 74F, adjacent to the early ecdysone-responsive region; however, the gene is not regulated by ecdysone at the level of transcription. The pattern of Pep expression through development suggests that maternal Pep gene transcripts are supplied to the embryo, and that the abundance of Pep gene transcripts decreases to a lower, fairly constant level thereafter. This unusual protein may play a role in the process of gene activation, or possibly in RNA processing, for a defined set of developmentally regulated loci.

journal_name

Genes Dev

journal_title

Genes & development

authors

Amero SA,Elgin SC,Beyer AL

doi

10.1101/gad.5.2.188

subject

Has Abstract

pub_date

1991-02-01 00:00:00

pages

188-200

issue

2

eissn

0890-9369

issn

1549-5477

journal_volume

5

pub_type

杂志文章
  • p27kip1 independently promotes neuronal differentiation and migration in the cerebral cortex.

    abstract::The generation of neurons by progenitor cells involves the tight coordination of multiple cellular activities, including cell cycle exit, initiation of neuronal differentiation, and cell migration. The mechanisms that integrate these different events into a coherent developmental program are not well understood. Here ...

    journal_title:Genes & development

    pub_type: 杂志文章

    doi:10.1101/gad.377106

    authors: Nguyen L,Besson A,Heng JI,Schuurmans C,Teboul L,Parras C,Philpott A,Roberts JM,Guillemot F

    更新日期:2006-06-01 00:00:00

  • Mad2 prevents aneuploidy and premature proteolysis of cyclin B and securin during meiosis I in mouse oocytes.

    abstract::In mitosis, the spindle checkpoint protein Mad2 averts aneuploidy by delaying anaphase onset until chromosomes align. Here we show that depletion of Mad2 in meiosis I mouse oocytes induced an increased incidence of aneuploidy. Proteolysis of cyclin B and securin commenced earlier in Mad2-depleted oocytes, resulting in...

    journal_title:Genes & development

    pub_type: 杂志文章

    doi:10.1101/gad.328105

    authors: Homer HA,McDougall A,Levasseur M,Yallop K,Murdoch AP,Herbert M

    更新日期:2005-01-15 00:00:00

  • Tandem AP-1-binding sites within the human beta-globin dominant control region function as an inducible enhancer in erythroid cells.

    abstract::A powerful enhancer has been mapped to an 18-bp DNA segment located 11 kb 5' to the human epsilon-globin gene within the dominant control or locus-activating region. This enhancer is inducible in K562 human erythroleukemia cells, increasing linked gamma-globin promoter/luciferase gene expression to 170-fold over an en...

    journal_title:Genes & development

    pub_type: 杂志文章

    doi:10.1101/gad.4.6.993

    authors: Ney PA,Sorrentino BP,McDonagh KT,Nienhuis AW

    更新日期:1990-06-01 00:00:00

  • Cap-independent translation of encephalomyocarditis virus RNA: structural elements of the internal ribosomal entry site and involvement of a cellular 57-kD RNA-binding protein.

    abstract::Translation of encephalomyocarditis virus (EMCV) mRNA occurs by ribosomal internal entry into the 5'-nontranslated region (5' NTR) rather than by ribosomal scanning. The internal ribosomal entry site (IRES) in the EMCV 5' NTR was determined by in vitro translation with RNAs that were generated by in vitro transcriptio...

    journal_title:Genes & development

    pub_type: 杂志文章

    doi:10.1101/gad.4.9.1560

    authors: Jang SK,Wimmer E

    更新日期:1990-09-01 00:00:00

  • The HIR corepressor complex binds to nucleosomes generating a distinct protein/DNA complex resistant to remodeling by SWI/SNF.

    abstract::The histone regulatory (HIR) and histone promoter control (HPC) repressor proteins regulate three of the four histone gene loci during the Saccharomyces cerevisiae cell cycle. Here, we demonstrate that Hir1, Hir2, Hir3, and Hpc2 proteins form a stable HIR repressor complex. The HIR complex promotes histone deposition ...

    journal_title:Genes & development

    pub_type: 杂志文章

    doi:10.1101/gad.1341105

    authors: Prochasson P,Florens L,Swanson SK,Washburn MP,Workman JL

    更新日期:2005-11-01 00:00:00

  • CBL family E3 ubiquitin ligases control JAK2 ubiquitination and stability in hematopoietic stem cells and myeloid malignancies.

    abstract::Janus kinase 2 (JAK2) is a central kinase in hematopoietic stem/progenitor cells (HSPCs), and its uncontrolled activation is a prominent oncogenic driver of hematopoietic neoplasms. However, molecular mechanisms underlying the regulation of JAK2 have remained elusive. Here we report that the Casitas B-cell lymphoma (C...

    journal_title:Genes & development

    pub_type: 杂志文章

    doi:10.1101/gad.297135.117

    authors: Lv K,Jiang J,Donaghy R,Riling CR,Cheng Y,Chandra V,Rozenova K,An W,Mohapatra BC,Goetz BT,Pillai V,Han X,Todd EA,Jeschke GR,Langdon WY,Kumar S,Hexner EO,Band H,Tong W

    更新日期:2017-05-15 00:00:00

  • Structural basis for distinct roles of SMAD2 and SMAD3 in FOXH1 pioneer-directed TGF-β signaling.

    abstract::TGF-β receptors phosphorylate SMAD2 and SMAD3 transcription factors, which then form heterotrimeric complexes with SMAD4 and cooperate with context-specific transcription factors to activate target genes. Here we provide biochemical and structural evidence showing that binding of SMAD2 to DNA depends on the conformati...

    journal_title:Genes & development

    pub_type: 杂志文章

    doi:10.1101/gad.330837.119

    authors: Aragón E,Wang Q,Zou Y,Morgani SM,Ruiz L,Kaczmarska Z,Su J,Torner C,Tian L,Hu J,Shu W,Agrawal S,Gomes T,Márquez JA,Hadjantonakis AK,Macias MJ,Massagué J

    更新日期:2019-11-01 00:00:00

  • TALE-light imaging reveals maternally guided, H3K9me2/3-independent emergence of functional heterochromatin in Drosophila embryos.

    abstract::Metazoans start embryogenesis with a relatively naïve genome. The transcriptionally inert, late-replicating heterochromatic regions, including the constitutive heterochromatin on repetitive sequences near centromeres and telomeres, need to be re-established during development. To explore the events initiating heteroch...

    journal_title:Genes & development

    pub_type: 杂志文章

    doi:10.1101/gad.272237.115

    authors: Yuan K,O'Farrell PH

    更新日期:2016-03-01 00:00:00

  • Genomic imprinting of Xist by maternal H3K27me3.

    abstract::Maternal imprinting at the Xist gene is essential to achieve paternal allele-specific imprinted X-chromosome inactivation (XCI) in female mammals. However, the mechanism underlying Xist imprinting is unclear. Here we show that the Xist locus is coated with a broad H3K27me3 domain that is established during oocyte grow...

    journal_title:Genes & development

    pub_type: 杂志文章

    doi:10.1101/gad.304113.117

    authors: Inoue A,Jiang L,Lu F,Zhang Y

    更新日期:2017-10-01 00:00:00

  • The function of Xenopus Bloom's syndrome protein homolog (xBLM) in DNA replication.

    abstract::The Bloom's syndrome gene (BLM) plays a pivotal role in the maintenance of genomic stability in somatic cells. It encodes a DNA helicase (BLM) of the RecQ family, but the exact function of BLM remains elusive. To study this question, we have cloned the BLM homolog of the frog Xenopus laevis (xBLM) and have raised anti...

    journal_title:Genes & development

    pub_type: 杂志文章

    doi:10.1101/gad.822400

    authors: Liao S,Graham J,Yan H

    更新日期:2000-10-15 00:00:00

  • Characterization of a murine homeo box gene, Hox-2.6, related to the Drosophila Deformed gene.

    abstract::The Hox-2 locus on chromosome 11 represents one of the major clusters of homeo-box-containing genes in the mouse. We have identified two new members (Hox-2.6 and Hox-2.7), which form part of this cluster of seven linked genes, and it appears that the Hox-2 locus is related by duplication and divergence to at least one...

    journal_title:Genes & development

    pub_type: 杂志文章

    doi:10.1101/gad.2.11.1424

    authors: Graham A,Papalopulu N,Lorimer J,McVey JH,Tuddenham EG,Krumlauf R

    更新日期:1988-11-01 00:00:00

  • Polar localization of the replication origin and terminus in Escherichia coli nucleoids during chromosome partitioning.

    abstract::We show the intracellular localization of the Escherichia coli replication origin (oriC) and chromosome terminus during the cell division cycle by FISH. In newborn cells, oriC is localized at the old-pole-proximal nucleoid border and the terminus at the new-pole-proximal nucleoid border. One copy of replicated oriC mi...

    journal_title:Genes & development

    pub_type: 杂志文章

    doi:10.1101/gad.12.7.1036

    authors: Niki H,Hiraga S

    更新日期:1998-04-01 00:00:00

  • A physiological role for gene loops in yeast.

    abstract::DNA loops that juxtapose the promoter and terminator regions of RNA polymerase II-transcribed genes have been identified in yeast and mammalian cells. Loop formation is transcription-dependent and requires components of the pre-mRNA 3'-end processing machinery. Here we report that looping at the yeast GAL10 gene persi...

    journal_title:Genes & development

    pub_type: 杂志文章

    doi:10.1101/gad.1823609

    authors: Lainé JP,Singh BN,Krishnamurthy S,Hampsey M

    更新日期:2009-11-15 00:00:00

  • Genomic predictors of interindividual differences in response to DNA damaging agents.

    abstract::Human lymphoblastoid cells derived from different healthy individuals display considerable variation in their transcription profiles. Here we show that such variation in gene expression underlies interindividual susceptibility to DNA damaging agents. The results demonstrate the massive differences in sensitivity acros...

    journal_title:Genes & development

    pub_type: 杂志文章

    doi:10.1101/gad.1688508

    authors: Fry RC,Svensson JP,Valiathan C,Wang E,Hogan BJ,Bhattacharya S,Bugni JM,Whittaker CA,Samson LD

    更新日期:2008-10-01 00:00:00

  • CDK12 phosphorylates 4E-BP1 to enable mTORC1-dependent translation and mitotic genome stability.

    abstract::The RNA polymerase II (RNAPII) C-terminal domain kinase, CDK12, regulates genome stability, expression of DNA repair genes, and cancer cell resistance to chemotherapy and immunotherapy. In addition to its role in mRNA biosynthesis of DNA repair genes, we show here that CDK12 phosphorylates the mRNA 5' cap-binding repr...

    journal_title:Genes & development

    pub_type: 杂志文章

    doi:10.1101/gad.322339.118

    authors: Choi SH,Martinez TF,Kim S,Donaldson C,Shokhirev MN,Saghatelian A,Jones KA

    更新日期:2019-04-01 00:00:00

  • Abro1 maintains genome stability and limits replication stress by protecting replication fork stability.

    abstract::Protection of the stalled replication fork is crucial for responding to replication stress and minimizing its impact on chromosome instability, thus preventing diseases, including cancer. We found a new component, Abro1, in the protection of stalled replication fork integrity. Abro1 deficiency results in increased chr...

    journal_title:Genes & development

    pub_type: 杂志文章

    doi:10.1101/gad.299172.117

    authors: Xu S,Wu X,Wu L,Castillo A,Liu J,Atkinson E,Paul A,Su D,Schlacher K,Komatsu Y,You MJ,Wang B

    更新日期:2017-07-15 00:00:00

  • CTD-dependent dismantling of the RNA polymerase II elongation complex by the pre-mRNA 3'-end processing factor, Pcf11.

    abstract::Pcf11 is one of numerous proteins involved in pre-mRNA 3'-end processing and transcription termination. Using elongation complexes (ECs) formed from purified yeast RNA polymerase II (Pol II), we show that a 140-amino acid polypeptide from yeast Pcf11 is capable of dismantling the EC in vitro. This action depends on th...

    journal_title:Genes & development

    pub_type: 杂志文章

    doi:10.1101/gad.1296305

    authors: Zhang Z,Fu J,Gilmour DS

    更新日期:2005-07-01 00:00:00

  • Drosophila ORC specifically binds to ACE3, an origin of DNA replication control element.

    abstract::In the yeast Saccharomyces cerevisiae, sequence-specific DNA binding by the origin recognition complex (ORC) is responsible for selecting origins of DNA replication. In metazoans, origin selection is poorly understood and it is unknown whether specific DNA binding by metazoan ORC controls replication. To address this ...

    journal_title:Genes & development

    pub_type: 杂志文章

    doi:10.1101/gad.13.20.2639

    authors: Austin RJ,Orr-Weaver TL,Bell SP

    更新日期:1999-10-15 00:00:00

  • Control of cell division by a retinoblastoma protein homolog in Chlamydomonas.

    abstract::A key pathway that controls both cell division and differentiation in animal cells is mediated by the retinoblastoma (RB) family of tumor suppressors, which gate the passage of cells from G(1) to S and through S phase. The role(s) of the RB pathway in plants are not yet clearly defined, nor has there been any evidence...

    journal_title:Genes & development

    pub_type: 杂志文章

    doi:10.1101/gad.892101

    authors: Umen JG,Goodenough UW

    更新日期:2001-07-01 00:00:00

  • The Dictyostelium MAP kinase ERK2 regulates multiple, independent developmental pathways.

    abstract::We showed previously that the MAP kinase ERK2 is essential for aggregation. erk2 null cells lack cAMP stimulation of adenylyl cyclase and thus cannot relay the cAMP chemotactic signal, although the cells chemotax to cAMP (Segall et al. 1995). In this paper we have examined the role of ERK2 in controlling developmental...

    journal_title:Genes & development

    pub_type: 杂志文章

    doi:10.1101/gad.10.1.118

    authors: Gaskins C,Clark AM,Aubry L,Segall JE,Firtel RA

    更新日期:1996-01-01 00:00:00

  • Stable nucleosome positioning and complete repression by the yeast alpha 2 repressor are disrupted by amino-terminal mutations in histone H4.

    abstract::Nucleosomes are positioned in the presence of the yeast repressor alpha 2 in minichromosomes containing the alpha 2 operator and on the promoters of a-cell-specific genes regulated by alpha 2. To investigate the possibility that alpha 2 directs nucleosome position through an interaction with a component of the core pa...

    journal_title:Genes & development

    pub_type: 杂志文章

    doi:10.1101/gad.6.3.411

    authors: Roth SY,Shimizu M,Johnson L,Grunstein M,Simpson RT

    更新日期:1992-03-01 00:00:00

  • Topoisomerases and yeast rRNA transcription: negative supercoiling stimulates initiation and topoisomerase activity is required for elongation.

    abstract::Previous work has shown that rRNA synthesis is strongly inhibited in yeast top1-top2 double mutants. Here, we show that inactivation of yeast topoisomerases can have paradoxical effects on transcription by RNA polymerase I. For example, transcription of ribosomal minigenes on extrachromosomal plasmids is greatly stimu...

    journal_title:Genes & development

    pub_type: 杂志文章

    doi:10.1101/gad.6.7.1332

    authors: Schultz MC,Brill SJ,Ju Q,Sternglanz R,Reeder RH

    更新日期:1992-07-01 00:00:00

  • SUMO-targeted ubiquitin E3 ligase RNF4 is required for the response of human cells to DNA damage.

    abstract::Here we demonstrate that RNF4, a highly conserved small ubiquitin-like modifier (SUMO)-targeted ubiquitin E3 ligase, plays a critical role in the response of mammalian cells to DNA damage. Human cells in which RNF4 expression was ablated by siRNA or chicken DT40 cells with a homozygous deletion of the RNF4 gene displa...

    journal_title:Genes & development

    pub_type: 杂志文章

    doi:10.1101/gad.189274.112

    authors: Yin Y,Seifert A,Chua JS,Maure JF,Golebiowski F,Hay RT

    更新日期:2012-06-01 00:00:00

  • Localization of RecA-like recombination proteins on chromosomes of the lily at various meiotic stages.

    abstract::The Rad51 and Lim15 proteins of lily, which are homologs of the bacterial RecA protein, were found on chromosomes in various stages of meiotic prophase 1. The presence of both Rad51 and Lim15 proteins as discrete foci on leptotene and zygotene chromosomes and their colocalization suggest that meiotic recombination beg...

    journal_title:Genes & development

    pub_type: 杂志文章

    doi:10.1101/gad.9.8.925

    authors: Terasawa M,Shinohara A,Hotta Y,Ogawa H,Ogawa T

    更新日期:1995-04-15 00:00:00

  • Xenopus cdc7 function is dependent on licensing but not on XORC, XCdc6, or CDK activity and is required for XCdc45 loading.

    abstract::The assembly and disassembly of protein complexes at replication origins play a crucial role in the regulation of chromosomal DNA replication. The sequential binding of the origin recognition complex (ORC), Cdc6, and the minichromosome maintenance (MCM/P1) proteins produces a licensed replication origin. Before the in...

    journal_title:Genes & development

    pub_type: 杂志文章

    doi:

    authors: Jares P,Blow JJ

    更新日期:2000-06-15 00:00:00

  • The stringent response in Myxococcus xanthus is regulated by SocE and the CsgA C-signaling protein.

    abstract::Myxococcus xanthus fruiting body development is induced by amino acid limitation. The decision to grow or develop is established by the RelA-dependent stringent response and A-signaling. We identified two new members of this regulatory hierarchy, socE and the C-signaling gene csgA. SocE depletion arrests growth and in...

    journal_title:Genes & development

    pub_type: 杂志文章

    doi:

    authors: Crawford EW Jr,Shimkets LJ

    更新日期:2000-02-15 00:00:00

  • Brg1 promotes both tumor-suppressive and oncogenic activities at distinct stages of pancreatic cancer formation.

    abstract::Pancreatic ductal adenocarcinoma (PDA) develops predominantly through pancreatic intraepithelial neoplasia (PanIN) and intraductal papillary mucinous neoplasm (IPMN) precursor lesions. Pancreatic acinar cells are reprogrammed to a "ductal-like" state during PanIN-PDA formation. Here, we demonstrate a parallel mechanis...

    journal_title:Genes & development

    pub_type: 杂志文章

    doi:10.1101/gad.256628.114

    authors: Roy N,Malik S,Villanueva KE,Urano A,Lu X,Von Figura G,Seeley ES,Dawson DW,Collisson EA,Hebrok M

    更新日期:2015-03-15 00:00:00

  • ClpS modulates but is not essential for bacterial N-end rule degradation.

    abstract::In eubacteria, the ClpS adaptor has been proposed to be essential for degradation of N-end rule substrates by the AAA(+) protease ClpAP. To test this model, we assayed degradation of substrates bearing N-end rule sequences isolated in a genetic screen for efficient degradation tags. ClpS was not vital for degradation ...

    journal_title:Genes & development

    pub_type: 杂志文章

    doi:10.1101/gad.1511907

    authors: Wang KH,Sauer RT,Baker TA

    更新日期:2007-02-15 00:00:00

  • (CA/GT)(n) microsatellites affect homologous recombination during yeast meiosis.

    abstract::One of the most common microsatellites in eukaryotes consists of tandem arrays of the dinucleotide GT. Although the study of the instability of such repetitive DNA has been extremely fruitful over the last decade, no biological function has been demonstrated for these sequences. We investigated the genetic behavior of...

    journal_title:Genes & development

    pub_type: 杂志文章

    doi:

    authors: Gendrel CG,Boulet A,Dutreix M

    更新日期:2000-05-15 00:00:00

  • Transcriptional activity of pannier is regulated negatively by heterodimerization of the GATA DNA-binding domain with a cofactor encoded by the u-shaped gene of Drosophila.

    abstract::The genes pannier (pnr) and u-shaped (ush) are required for the regulation of achaete-scute during establishment of the bristle pattern in Drosophila. pnr encodes a protein belonging to the GATA family of transcription factors, whereas ush encodes a novel zinc finger protein. Genetic interactions between dominant pnr ...

    journal_title:Genes & development

    pub_type: 杂志文章

    doi:10.1101/gad.11.22.3096

    authors: Haenlin M,Cubadda Y,Blondeau F,Heitzler P,Lutz Y,Simpson P,Ramain P

    更新日期:1997-11-15 00:00:00