Abstract:
:Codon usage bias is a universal feature of all genomes, but its in vivo biological functions in animal systems are not clear. To investigate the in vivo role of codon usage in animals, we took advantage of the sensitivity and robustness of the Drosophila circadian system. By codon-optimizing parts of Drosophila period (dper), a core clock gene that encodes a critical component of the circadian oscillator, we showed that dper codon usage is important for circadian clock function. Codon optimization of dper resulted in conformational changes of the dPER protein, altered dPER phosphorylation profile and stability, and impaired dPER function in the circadian negative feedback loop, which manifests into changes in molecular rhythmicity and abnormal circadian behavioral output. This study provides an in vivo example that demonstrates the role of codon usage in determining protein structure and function in an animal system. These results suggest a universal mechanism in eukaryotes that uses a codon usage "code" within genetic codons to regulate cotranslational protein folding.
journal_name
Genes Devjournal_title
Genes & developmentauthors
Fu J,Murphy KA,Zhou M,Li YH,Lam VH,Tabuloc CA,Chiu JC,Liu Ydoi
10.1101/gad.281030.116subject
Has Abstractpub_date
2016-08-01 00:00:00pages
1761-75issue
15eissn
0890-9369issn
1549-5477pii
30/15/1761journal_volume
30pub_type
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