Abstract：
:During gastrulation of Xenopus embryos the prospective mesoderm is induced initially with domains of dorsal and ventral fate, then further patterned to generate somitic mesoderm by signals from the gastrula organizer. Although Xwnt-8 is expressed in future ventrolateral mesoderm and induces prospective epidermis to differentiate in vitro as ventral mesoderm, no loss-of-function studies have demonstrated a requirement for Wnt signaling for the normal expression of mesodermal genes in the gastrula. We report development of a dominant-negative Wnt (dnXwnt-8) that inhibits embryonic responses to Wnt signaling in a cell-nonautonomous fashion. By expressing dnXwnt-8 in Xenopus embryos, we uncover a requirement of Wnt signaling for localized expression in prospective mesoderm of XMyoDa and Xenopus-posterior (Xpo). Because ectopic expression of functional Xwnt-8 in the dorsal marginal zone of the gastrula induces ectopic XMyoDa and Xpo, both gain-of-function and loss-of-function experiments support a model in which endogenous Xwnt-8 functions to induce expression of genes involved in specification of ventral and somitic mesoderm.
journal_name
Genes Devjournal_title
Genes & developmentauthors
Hoppler S,Brown JD,Moon RTdoi
10.1101/gad.10.21.2805subject
Has Abstractpub_date
1996-11-01 00:00:00pages
2805-17issue
21eissn
0890-9369issn
1549-5477journal_volume
10pub_type
杂志文章abstract：:Transcription of the cell-cycle-regulated human histone genes increases approximately fivefold during S phase. One step toward the elucidation of the biochemical mechanisms that govern cell-cycle-regulated expression of these genes is to purify and characterize the transcription factors that regulate these promoters. ...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.2.12b.1700
更新日期：1988-12-01 00:00:00
abstract：:TDP-43 is a critical RNA-binding factor associated with pre-mRNA splicing in mammals. Its expression is tightly autoregulated, with loss of this regulation implicated in human neuropathology. We demonstrate that TDP-43 overexpression in humans and mice activates a 3' untranslated region (UTR) intron, resulting in exci...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.194829.112
更新日期：2012-08-01 00:00:00
abstract：:The enzyme telomerase, which elongates chromosome termini, is a critical factor in determining long-term cellular proliferation and tissue renewal. Hence, even small differences in telomerase levels can have substantial consequences for human health. In budding yeast, telomerase consists of the catalytic Est2 protein ...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.246256.114
更新日期：2014-10-01 00:00:00
abstract：:Transformation of primary rodent cells by the adenovirus E1A and E1B oncogenes is a two-step process, where E1A-dependent induction of proliferation is coupled to E1B-dependent suppression of programmed cell death (apoptosis). The E1B gene encodes two distinct transforming proteins, the 19K and 55K proteins, both of w...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.7.4.546
更新日期：1993-04-01 00:00:00
abstract：:The activity of the polyoma virus (PyV) origin of DNA replication was used as a sensitive assay for enhancer function in one- and two-cell mouse embryos by injecting embryos with plasmid DNA containing different PyV ori configurations, allowing them to continue development in vitro, and then measuring plasmid DNA repl...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.2.9.1115
更新日期：1988-09-01 00:00:00
abstract：:Maternal imprinting at the Xist gene is essential to achieve paternal allele-specific imprinted X-chromosome inactivation (XCI) in female mammals. However, the mechanism underlying Xist imprinting is unclear. Here we show that the Xist locus is coated with a broad H3K27me3 domain that is established during oocyte grow...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.304113.117
更新日期：2017-10-01 00:00:00
abstract：:The target gene(s) required for Myc-mediated tumorigenesis are still elusive. Here we show that while endogenous c-Myc is surprisingly dispensable for skin homeostasis and TPA-induced hyperplasia, c-Myc-deficient epidermis is resistant to Ras-mediated DMBA/TPAinduced tumorigenesis. This is mechanistically linked to p2...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.381206
更新日期：2006-08-01 00:00:00
abstract：:Animals alter their reproductive programs to accommodate changes in nutrient availability, yet the connections between known nutrient-sensing systems and reproductive programs are underexplored, and whether there is a mechanism that senses nucleotide levels to coordinate germline proliferation is unknown. We establish...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.275107.115
更新日期：2016-02-01 00:00:00
abstract：:The orphan nuclear receptor TLX regulates neural stem cell self-renewal in the adult brain and functions primarily as a transcription repressor through recruitment of Atrophin corepressors, which bind to TLX via a conserved peptide motif termed the Atro box. Here we report crystal structures of the human and insect TL...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.254904.114
更新日期：2015-02-15 00:00:00
abstract：:The assembly of activated RNA polymerase II (pol II) transcription complexes has been investigated by assaying whether pre-assembly of intermediate complexes reduces the extended time required for start-site melting. The results show that a closed complex requiring factors IIA, IID, and the acidic activator GAL4-AH fo...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.6.9.1716
更新日期：1992-09-01 00:00:00
abstract：:Genetic and cytologic data from Saccharomyces cerevisiae and mammals implicate the Mre11 complex, consisting of Mre11, Rad50, and Nbs1, as a sensor of DNA damage, and indicate that the complex influences the activity of ataxia-telangiectasia mutated (ATM) in the DNA damage response. Rad50(S/S) mice exhibit precipitous...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.1373705
更新日期：2005-12-15 00:00:00
abstract：:The N-myc gene encodes a putative transcription factor that is thought to function in the regulation of gene expression during cell differentiation and/or growth. To examine the role of N-myc during development, we have used targeted mutagenesis in embryonic stem cells to produce a mouse line that carries an N-myc nul...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.6.12a.2248
更新日期：1992-12-01 00:00:00
abstract：:The ultraviolet (UV)-induced (6-4) pyrimidine-pyrimidone photoproduct [(6-4) PP] confers a large structural distortion in DNA. Here we examine in human cells the roles of translesion synthesis (TLS) DNA polymerases (Pols) in promoting replication through a (6-4) TT photoproduct carried on a duplex plasmid where bidire...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.1872810
更新日期：2010-01-15 00:00:00
abstract：:Male sexual development in the nematode Caenorhabditis elegans requires the genes fem-1, fem-2, and fem-3. The current model of sex determination portrays the FEM proteins as components of a novel signal transduction pathway, but the mechanisms involved in signaling through the pathway are not understood. We report th...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.10.18.2314
更新日期：1996-09-15 00:00:00
abstract：:Gut homeostasis is controlled by both immune and developmental mechanisms, and its disruption can lead to inflammatory disorders or cancerous lesions of the intestine. While the impact of bacteria on the mucosal immune system is beginning to be precisely understood, little is known about the effects of bacteria on gut...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.1827009
更新日期：2009-10-01 00:00:00
abstract：:Mu DNA transposition proceeds through a series of higher-order nucleoprotein complexes called transpososomes. The structural core of the transpososome is a tetramer of the transposase, Mu A, bound to the two transposon ends. High-resolution structural analysis of the intact transposase and the transpososome has not be...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.1291405
更新日期：2005-04-01 00:00:00
abstract：:Checkpoint genes maintain genomic stability by arresting cells after DNA damage. Many of these genes also control cell cycle events in unperturbed cells. By conducting a screen for checkpoint genes in zebrafish, we found that dtl/cdt2 is an essential component of the early, radiation-induced G2/M checkpoint. We subseq...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.1482106
更新日期：2006-11-15 00:00:00
abstract：:The liver is a unique organ with a remarkably high potential to regenerate upon injuries. In severely damaged livers where hepatocyte proliferation is impaired, facultative liver progenitor cells (LPCs) proliferate and are assumed to contribute to regeneration. An expansion of LPCs is often observed in patients with v...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.204776.112
更新日期：2013-01-15 00:00:00
abstract：:Known prions (infectious proteins) are self-propagating amyloids or conformationally altered proteins, but in theory an enzyme necessary for its own activation could also be a prion (or a gene composed of protein). We show that yeast protease B is such a prion, called [beta].[beta] is infectious, reversibly curable, a...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.1115803
更新日期：2003-09-01 00:00:00
abstract：:Cascades of zygotic gene expression pattern the anterior-posterior (AP) and dorsal-ventral (DV) axes of the early Drosophila embryo. Here, we used the global run-on sequencing assay (GRO-seq) to map the genome-wide RNA polymerase distribution during early Drosophila embryogenesis, thus providing insights into how gene...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.215459.113
更新日期：2013-05-15 00:00:00
abstract：:The ATR (ATM and Rad3-related) kinase and its regulatory partner ATRIP (ATR-interacting protein) coordinate checkpoint responses to DNA damage and replication stress. TopBP1 functions as a general activator of ATR. However, the mechanism by which TopBP1 activates ATR is unknown. Here, we show that ATRIP contains a Top...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.1666208
更新日期：2008-06-01 00:00:00
abstract：:During vascular development, endothelial platelet-derived growth factor B (PDGF-B) is critical for pericyte recruitment. Deletion of the conserved C-terminal heparin-binding motif impairs PDGF-BB retention and pericyte recruitment in vivo, suggesting a potential role for heparan sulfate (HS) in PDGF-BB function during...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.398207
更新日期：2007-02-01 00:00:00
abstract：:Eukaryotic genomes encode large families of deubiquitinating enzymes (DUBs). Genetic data suggest that Fat facets (Faf), a Drosophila DUB essential for patterning the compound eye, might have a novel regulatory function; Faf might reverse the ubiquitination of a specific substrate, thereby preventing proteasomal degra...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.961502
更新日期：2002-02-01 00:00:00
abstract：:SET8 (also known as PR-SET7) is a histone H4-Lys-20-specific methyltransferase that is implicated in cell-cycle-dependent transcriptional silencing and mitotic regulation in metazoans. Herein we report the crystal structure of human SET8 (hSET8) bound to a histone H4 peptide bearing Lys-20 and the product cofactor S-a...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.1318405
更新日期：2005-06-15 00:00:00
abstract：:Mammalian histone gene transcription is increased approximately fivefold during the transition from the G1 phase to the S phase of the cell cycle. In this study, we present a detailed in vivo analysis of the human histone H2b promoter, which establishes that transcriptional regulation of this gene is mediated by a sub...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.2.1.32
更新日期：1988-01-01 00:00:00
abstract：:Although branchpoint recognition is an essential component of intron excision during the RNA splicing process, the branchpoint itself is frequently assumed to be a basal, rather than regulatory, sequence feature. However, this assumption has not been systematically tested due to the technical difficulty of identifying...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.312058.118
更新日期：2018-04-01 00:00:00
abstract：:The serine/threonine kinase Akt/PKB is a major downstream effector of growth factor-mediated cell survival. Activated Akt, like Bcl-2 and Bcl-xL, prevents closure of a PT pore component, the voltage-dependent anion channel (VDAC); intracellular acidification; mitochondrial hyperpolarization; and the decline in oxidati...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.889901
更新日期：2001-06-01 00:00:00
abstract：:Sox2 is expressed in basal epithelial cells of the tongue, with high levels in taste bud placodes, fungiform papillae, and mature taste cells, and low levels in filiform papillae. High Sox2 expression appears to lie downstream from canonical Wnt signaling. In hypomorphic Sox2(EGFP/LP) embryos, placodes form but no mat...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.1457106
更新日期：2006-10-01 00:00:00
abstract：:The Rad51 and Lim15 proteins of lily, which are homologs of the bacterial RecA protein, were found on chromosomes in various stages of meiotic prophase 1. The presence of both Rad51 and Lim15 proteins as discrete foci on leptotene and zygotene chromosomes and their colocalization suggest that meiotic recombination beg...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.9.8.925
更新日期：1995-04-15 00:00:00
abstract：:The liver X receptors (LXRs) are members of the nuclear hormone receptor superfamily that are bound and activated by oxysterols. These receptors serve as sterol sensors to regulate the transcription of gene products that control intracellular cholesterol homeostasis through catabolism and transport. In this report, we...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.844900
更新日期：2000-11-15 00:00:00
