Abstract:
:We have purified and cloned a new splicing factor, KSRP. KSRP is a component of a multiprotein complex that binds specifically to an intronic splicing enhancer element downstream of the neuron-specific c-src N1 exon. This 75-kD protein induces the assembly of five other proteins, including the heterogeneous nuclear ribonucleoprotein F, onto the splicing enhancer. The sequence of the KSRP cDNA indicates that the protein contains four K homology RNA-binding domains and an unusual carboxy-terminal domain. KSRP is similar to two proteins, FUSE-binding protein and P-element somatic inhibitor. KSRP is expressed in both neural and non-neural cell lines, although it is present at higher levels in neural cells. Antibodies specific for KSRP inhibit the splicing of the N1 exon in vitro. Moreover, this inhibition of N1 splicing can be rescued by the addition of purified KSRP. KSRP is likely to regulate splicing from a number of intronic splicing enhancer sequences.
journal_name
Genes Devjournal_title
Genes & developmentauthors
Min H,Turck CW,Nikolic JM,Black DLdoi
10.1101/gad.11.8.1023subject
Has Abstractpub_date
1997-04-15 00:00:00pages
1023-36issue
8eissn
0890-9369issn
1549-5477journal_volume
11pub_type
杂志文章abstract::The p53 inhibitor murine double-minute gene 2 (Mdm2) is a target for potential cancer therapies, however increased p53 function can be lethal. To directly address whether reduced Mdm2 function can inhibit tumorigenesis without causing detrimental side effects, we exploited a hypomorphic murine allele of mdm2 to compar...
journal_title:Genes & development
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journal_title:Genes & development
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journal_title:Genes & development
pub_type: 杂志文章,评审
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pub_type: 杂志文章
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更新日期:2002-09-01 00:00:00
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pub_type: 杂志文章
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更新日期:2001-12-15 00:00:00
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journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.917101
更新日期:2001-11-01 00:00:00
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journal_title:Genes & development
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journal_title:Genes & development
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journal_title:Genes & development
pub_type: 杂志文章
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更新日期:2000-04-15 00:00:00
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journal_title:Genes & development
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journal_title:Genes & development
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更新日期:2000-08-15 00:00:00
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journal_title:Genes & development
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