Abstract:
:Protein kinases are key regulators of cellular processes. In spite of considerable effort, a full understanding of the pathways they participate in remains elusive. We globally investigated the proteins that interact with the majority of yeast protein kinases using protein microarrays. Eighty-five kinases were purified and used to probe yeast proteome microarrays. One-thousand-twenty-three interactions were identified, and the vast majority were novel. Coimmunoprecipitation experiments indicate that many of these interactions occurred in vivo. Many novel links of kinases to previously distinct cellular pathways were discovered. For example, the well-studied Kss1 filamentous pathway was found to bind components of diverse cellular pathways, such as those of the stress response pathway and the Ccr4-Not transcriptional/translational regulatory complex; genetic tests revealed that these different components operate in the filamentation pathway in vivo. Overall, our results indicate that kinases operate in a highly interconnected network that coordinates many activities of the proteome. Our results further demonstrate that protein microarrays uncover a diverse set of interactions not observed previously.
journal_name
Genes Devjournal_title
Genes & developmentauthors
Fasolo J,Sboner A,Sun MG,Yu H,Chen R,Sharon D,Kim PM,Gerstein M,Snyder Mdoi
10.1101/gad.1998811subject
Has Abstractpub_date
2011-04-01 00:00:00pages
767-78issue
7eissn
0890-9369issn
1549-5477pii
25/7/767journal_volume
25pub_type
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