The characterization of the TFIIIA synthesized in somatic cells of Xenopus laevis.


:In somatic cells of Xenopus, transcription of the TFIIIA gene initiates greater than 200 bp upstream from the start site used in oocytes. The resultant mRNA encodes a protein, S-TFIIIA, that is 22 amino acids longer at its amino terminus than the abundant form of TFIIIA in oocytes (O-TFIIIA). S-TFIIIA binds the 5S RNA gene and 5S RNA, and both O- and S-TFIIIA promote the formation of stable transcription complexes on oocyte-type 5S RNA genes in an oocyte nuclear extract. We have not found any functional difference between the two forms of TFIIIA. Different transcription start sites suggest differential promoter usage--one in oocytes that permits high levels of gene activity and another that is used in somatic cells for low-level TFIIIA mRNA synthesis.


Genes Dev


Genes & development


Kim SH,Darby MK,Joho KE,Brown DD




Has Abstract


1990-09-01 00:00:00












  • The orphan nuclear receptor COUP-TFII is required for angiogenesis and heart development.

    abstract::The embryonic expression of COUP-TFII, an orphan nuclear receptor, suggests that it may participate in mesenchymal-epithelial interactions required for organogenesis. Targeted deletion of the COUP-TFII gene results in embryonic lethality with defects in angiogenesis and heart development. COUP-TFII mutants are defecti...

    journal_title:Genes & development

    pub_type: 杂志文章


    authors: Pereira FA,Qiu Y,Zhou G,Tsai MJ,Tsai SY

    更新日期:1999-04-15 00:00:00

  • Lysine methylation within the globular domain of histone H3 by Dot1 is important for telomeric silencing and Sir protein association.

    abstract::The amino-terminal histone tails are subject to covalent post-translational modifications such as acetylation, methylation, and phosphorylation. In the histone code hypothesis, these exposed and unstructured histone tails are accessible to a repertoire of regulatory factors that specifically recognize the various modi...

    journal_title:Genes & development

    pub_type: 杂志文章


    authors: Ng HH,Feng Q,Wang H,Erdjument-Bromage H,Tempst P,Zhang Y,Struhl K

    更新日期:2002-06-15 00:00:00

  • cAMP stimulates the C/EBP-related transcription factor rNFIL-6 to trans-locate to the nucleus and induce c-fos transcription.

    abstract::The c-fos serum response element (SRE) is a multifunctional regulatory region of the c-fos promoter that responds to a variety of inducers. Recently, we have demonstrated that the SRE binds the C/EBP-related transcription factor rat NFIL-6 (rNFIL-6). In this study we show that rNFIL-6 is regulated by the cAMP second m...

    journal_title:Genes & development

    pub_type: 杂志文章


    authors: Metz R,Ziff E

    更新日期:1991-10-01 00:00:00

  • Repression of p53-mediated transcription by MDM2: a dual mechanism.

    abstract::The oncoprotein MDM2 binds to the activation domain of the tumor suppressor p53 and inhibits its ability to stimulate transcription. This same region of p53 is able to bind several basal transcription factors that appear to be important for the transactivation function of p53. It has therefore been suggested that MDM2...

    journal_title:Genes & development

    pub_type: 杂志文章


    authors: Thut CJ,Goodrich JA,Tjian R

    更新日期:1997-08-01 00:00:00

  • The secE gene encodes an integral membrane protein required for protein export in Escherichia coli.

    abstract::Genetic screening and selection procedures employing a secA-lacZ fusion strain repeatedly have yielded mutations in four genes affecting the protein export pathway of Escherichia coli. These genes are secA, secD, prlA/secY, and secE. We discuss the significance of the failure to find new sec genes after extensive use ...

    journal_title:Genes & development

    pub_type: 杂志文章


    authors: Schatz PJ,Riggs PD,Jacq A,Fath MJ,Beckwith J

    更新日期:1989-07-01 00:00:00

  • SMARCB1 loss interacts with neuronal differentiation state to block maturation and impact cell stability.

    abstract::Atypical teratoid rhabdoid tumors (ATRTs) are challenging pediatric brain cancers that are predominantly associated with inactivation of the gene SMARCB1, a conserved subunit of the chromatin remodeling BAF complex, which has known contributions to developmental processes. To identify potential interactions between SM...

    journal_title:Genes & development

    pub_type: 杂志文章


    authors: Parisian AD,Koga T,Miki S,Johann PD,Kool M,Crawford JR,Furnari FB

    更新日期:2020-10-01 00:00:00

  • Identification of the sigma E subunit of Escherichia coli RNA polymerase: a second alternate sigma factor involved in high-temperature gene expression.

    abstract::The rpoH gene of Escherichia coli encodes sigma 32, the 32-kD sigma-factor responsible for the heat-inducible transcription of the heat shock genes. rpoH is transcribed from at least three promoters. Two of these promoters are recognized by RNA polymerase containing sigma 70, the predominant sigma-factor. We purified ...

    journal_title:Genes & development

    pub_type: 杂志文章


    authors: Erickson JW,Gross CA

    更新日期:1989-09-01 00:00:00

  • Activation of the nuclear factor-kappaB by Rho, CDC42, and Rac-1 proteins.

    abstract::The Rho family of small GTPases are critical elements involved in the regulation of signal transduction cascades from extracellular stimuli to the cell nucleus, including the JNK/SAPK signaling pathway, the c-fos serum response factor, and the p70 S6 kinase. Here we report a novel signaling pathway activated by the Rh...

    journal_title:Genes & development

    pub_type: 杂志文章


    authors: Perona R,Montaner S,Saniger L,Sánchez-Pérez I,Bravo R,Lacal JC

    更新日期:1997-02-15 00:00:00

  • Induction of cell fate in the Drosophila retina: the bride of sevenless protein is predicted to contain a large extracellular domain and seven transmembrane segments.

    abstract::Previous genetic mosaic studies established that expression of the Drosophila bride of sevenless (boss) gene is required in photoreceptor neuron R8 for the development of photoreceptor neuron R7. This led to the proposal that boss encodes or regulates an R7-specific inductive cue. We have identified the boss gene base...

    journal_title:Genes & development

    pub_type: 杂志文章


    authors: Hart AC,Krämer H,Van Vactor DL Jr,Paidhungat M,Zipursky SL

    更新日期:1990-11-01 00:00:00

  • Changes in histone gene dosage alter transcription in yeast.

    abstract::Chromatin structure is believed to be important for a number of cellular processes, including transcription. However, the role of nucleosomes in transcription is not well understood. We have identified the yeast histone locus HTB1-HTB1, encoding histones H2A and H2B, as a suppressor of solo delta insertion mutations t...

    journal_title:Genes & development

    pub_type: 杂志文章


    authors: Clark-Adams CD,Norris D,Osley MA,Fassler JS,Winston F

    更新日期:1988-02-01 00:00:00

  • The Drosophila abrupt gene encodes a BTB-zinc finger regulatory protein that controls the specificity of neuromuscular connections.

    abstract::Motor axons make synaptic connections with specific muscles, and this specificity unfolds during development as motoneuron growth cones make specific pathway choices and ultimately recognize and synapse on their specific muscle targets. The Drosophila clueless mutation was identified previously in a genetic screen for...

    journal_title:Genes & development

    pub_type: 杂志文章


    authors: Hu S,Fambrough D,Atashi JR,Goodman CS,Crews ST

    更新日期:1995-12-01 00:00:00

  • Mitochondrial Mg(2+) homeostasis is critical for group II intron splicing in vivo.

    abstract::The product of the nuclear MRS2 gene, Mrs2p, is the only candidate splicing factor essential for all group II introns in mitochondria of the yeast Saccharomyces cerevisiae. It has been shown to be an integral protein of the inner mitochondrial membrane, structurally and functionally related to the bacterial CorA Mg(2+...

    journal_title:Genes & development

    pub_type: 杂志文章


    authors: Gregan J,Kolisek M,Schweyen RJ

    更新日期:2001-09-01 00:00:00

  • Development of neuroendocrine lineages requires the bHLH-PAS transcription factor SIM1.

    abstract::The bHLH-PAS transcription factor SIM1 is expressed during the development of the hypothalamic-pituitary axis in three hypothalamic nuclei: the paraventricular nucleus (PVN), the anterior periventricular nucleus (aPV), and the supraoptic nucleus (SON). To investigate Sim1 function in the hypothalamus, we produced mice...

    journal_title:Genes & development

    pub_type: 杂志文章


    authors: Michaud JL,Rosenquist T,May NR,Fan CM

    更新日期:1998-10-15 00:00:00

  • Activities of Ligatin and MCT-1/DENR in eukaryotic translation initiation and ribosomal recycling.

    abstract::Eukaryotic translation initiation begins with ribosomal recruitment of aminoacylated initiator tRNA (Met-tRNA(Met)(i)) by eukaryotic initiation factor eIF2. In cooperation with eIF3, eIF1, and eIF1A, Met-tRNA(Met)(i)/eIF2/GTP binds to 40S subunits yielding 43S preinitiation complexes that attach to the 5'-terminal reg...

    journal_title:Genes & development

    pub_type: 杂志文章


    authors: Skabkin MA,Skabkina OV,Dhote V,Komar AA,Hellen CU,Pestova TV

    更新日期:2010-08-15 00:00:00

  • A nonproteolytic function of the proteasome is required for the dissociation of Cdc2 and cyclin B at the end of M phase.

    abstract::Inactivation of cyclin B-Cdc2 kinase at the exit from M phase depends on the specific proteolysis of the cyclin B subunit, whereas the Cdc2 subunit remains present at nearly constant levels throughout the cell cycle. It is unknown how Cdc2 escapes degradation when cyclin B is destroyed. In Xenopus egg extracts that re...

    journal_title:Genes & development

    pub_type: 杂志文章


    authors: Nishiyama A,Tachibana K,Igarashi Y,Yasuda H,Tanahashi N,Tanaka K,Ohsumi K,Kishimoto T

    更新日期:2000-09-15 00:00:00

  • The crystal structure of DR6 in complex with the amyloid precursor protein provides insight into death receptor activation.

    abstract::The amyloid precursor protein (APP) has garnered considerable attention due to its genetic links to Alzheimer's disease. Death receptor 6 (DR6) was recently shown to bind APP via the protein extracellular regions, stimulate axonal pruning, and inhibit synapse formation. Here, we report the crystal structure of the DR6...

    journal_title:Genes & development

    pub_type: 杂志文章


    authors: Xu K,Olsen O,Tzvetkova-Robev D,Tessier-Lavigne M,Nikolov DB

    更新日期:2015-04-15 00:00:00

  • A master CLOCK hard at work brings rhythm to the transcriptome.

    abstract::In this issue of Genes & Development, Abruzzi et al. (pp. 2374-2386) use chromatin immunoprecipitation (ChIP) tiling array assays (ChIP-chip) to show that physical interactions between circadian (≅24-h) clock machineries and genomes are more widespread than previously thought and provide novel insights into how clocks...

    journal_title:Genes & development

    pub_type: 评论,杂志文章


    authors: Edery I

    更新日期:2011-11-15 00:00:00

  • ICP27 mediates HSV RNA export by shuttling through a leucine-rich nuclear export signal and binding viral intronless RNAs through an RGG motif.

    abstract::Infection of metazoan cells with some viruses alters the balance of cellular mRNA export to favor viral RNA export and to retain cellular transcripts in the nucleus. Here, evidence is presented to show that the herpes simplex virus 1 (HSV-1) essential regulatory protein ICP27, which inhibits host cell-splicing, result...

    journal_title:Genes & development

    pub_type: 杂志文章


    authors: Sandri-Goldin RM

    更新日期:1998-03-15 00:00:00

  • The acidic activator GAL4-AH can stimulate polymerase II transcription by promoting assembly of a closed complex requiring TFIID and TFIIA.

    abstract::The assembly of activated RNA polymerase II (pol II) transcription complexes has been investigated by assaying whether pre-assembly of intermediate complexes reduces the extended time required for start-site melting. The results show that a closed complex requiring factors IIA, IID, and the acidic activator GAL4-AH fo...

    journal_title:Genes & development

    pub_type: 杂志文章


    authors: Wang W,Gralla JD,Carey M

    更新日期:1992-09-01 00:00:00

  • A novel class of small RNAs in mouse spermatogenic cells.

    abstract::Small noncoding RNAs, including small interfering RNAs (siRNAs) and micro RNAs (miRNAs) of approximately 21 nucleotides (nt) in length, have emerged as potent regulators of gene expression at both transcriptional and post-transcriptional levels in diverse organisms. Here we report the identification of a novel class o...

    journal_title:Genes & development

    pub_type: 杂志文章


    authors: Grivna ST,Beyret E,Wang Z,Lin H

    更新日期:2006-07-01 00:00:00

  • The embryonic lethality of homozygous lethal yellow mice (Ay/Ay) is associated with the disruption of a novel RNA-binding protein.

    abstract::Lethal yellow (Ay) is a mutation at the mouse agouti (a) locus that is associated with an all-yellow coat color, obesity, diabetes, tumors in heterozygotes, and preimplantation embryonic lethality in homozygotes. Previously, we cloned and characterized the wild-type agouti gene and demonstrated that it expresses a 0.8...

    journal_title:Genes & development

    pub_type: 杂志文章


    authors: Michaud EJ,Bultman SJ,Stubbs LJ,Woychik RP

    更新日期:1993-07-01 00:00:00

  • Tight protein-DNA interactions favor gene silencing.

    abstract::The heterochromatin-like structure formed by the yeast silent information regulator complex (SIR) represses transcription at the silent mating type loci and telomeres. Here, we report that tight protein-DNA complexes induce ectopic recruitment of the SIR complex, promoting gene silencing and changes in subnuclear loca...

    journal_title:Genes & development

    pub_type: 杂志文章


    authors: Dubarry M,Loïodice I,Chen CL,Thermes C,Taddei A

    更新日期:2011-07-01 00:00:00

  • Sonic hedgehog acts cell-autonomously on muscle precursor cells to generate limb muscle diversity.

    abstract::How muscle diversity is generated in the vertebrate body is poorly understood. In the limb, dorsal and ventral muscle masses constitute the first myogenic diversification, as each gives rise to distinct muscles. Myogenesis initiates after muscle precursor cells (MPCs) have migrated from the somites to the limb bud and...

    journal_title:Genes & development

    pub_type: 杂志文章


    authors: Anderson C,Williams VC,Moyon B,Daubas P,Tajbakhsh S,Buckingham ME,Shiroishi T,Hughes SM,Borycki AG

    更新日期:2012-09-15 00:00:00

  • Activation of Rac1 by a Crk SH3-binding protein, DOCK180.

    abstract::DOCK180 is involved in integrin signaling through CrkII-p130(Cas) complexes. We have studied the involvement of DOCK180 in Rac1 signaling cascades. DOCK180 activated JNK in a manner dependent on Rac1, Cdc42Hs, and SEK, and overexpression of DOCK180 increased the amount of GTP-bound Rac1 in 293T cells. Coexpression of ...

    journal_title:Genes & development

    pub_type: 杂志文章


    authors: Kiyokawa E,Hashimoto Y,Kobayashi S,Sugimura H,Kurata T,Matsuda M

    更新日期:1998-11-01 00:00:00

  • RNase E action at a distance: degradation of target mRNAs mediated by an Hfq-binding small RNA in bacteria.

    abstract::A major class of bacterial small RNAs (sRNAs), along with RNA-binding protein Hfq and endoribonuclease RNase E, acts on target mRNAs through base-pairing, leading to translational repression and rapid degradation of the mRNAs. In this issue of Genes & Development, Prévost and colleagues (pp. 385-396) demonstrate by us...

    journal_title:Genes & development

    pub_type: 评论,杂志文章


    authors: Morita T,Aiba H

    更新日期:2011-02-15 00:00:00

  • Drosophila lacking microRNA miR-278 are defective in energy homeostasis.

    abstract::The mechanisms that control energy homeostasis and tissue growth during development are closely linked through the insulin signal transduction pathway. Changes in the level of insulin and other hormones reflect the nutritional status of the animal to control circulating sugar levels and fat metabolism. Systemic defect...

    journal_title:Genes & development

    pub_type: 杂志文章


    authors: Teleman AA,Maitra S,Cohen SM

    更新日期:2006-02-15 00:00:00

  • Noncoding RNA control of the making and breaking of sugars.

    abstract::Noncoding RNA regulators have been implicated in almost all imaginable cellular processes. Here we review how regulatory small RNAs such as Spot42, SgrS, GlmY, and GlmZ and a cis-encoded ribozyme in glmS mRNA control sugar metabolism. Besides discussing the physiological implications, we show how the study of these mo...

    journal_title:Genes & development

    pub_type: 杂志文章,评审


    authors: Görke B,Vogel J

    更新日期:2008-11-01 00:00:00

  • Invasive and indigenous microbiota impact intestinal stem cell activity through multiple pathways in Drosophila.

    abstract::Gut homeostasis is controlled by both immune and developmental mechanisms, and its disruption can lead to inflammatory disorders or cancerous lesions of the intestine. While the impact of bacteria on the mucosal immune system is beginning to be precisely understood, little is known about the effects of bacteria on gut...

    journal_title:Genes & development

    pub_type: 杂志文章


    authors: Buchon N,Broderick NA,Chakrabarti S,Lemaitre B

    更新日期:2009-10-01 00:00:00

  • Dynamic expression and essential functions of Hes7 in somite segmentation.

    abstract::The basic helix-loop-helix (bHLH) gene Hes7, a putative Notch effector, encodes a transcriptional repressor. Here, we found that Hes7 expression oscillates in 2-h cycles in the presomitic mesoderm (PSM). In Hes7-null mice, somites are not properly segmented and their anterior-posterior polarity is disrupted. As a resu...

    journal_title:Genes & development

    pub_type: 杂志文章


    authors: Bessho Y,Sakata R,Komatsu S,Shiota K,Yamada S,Kageyama R

    更新日期:2001-10-15 00:00:00

  • Translational inactivation of ribosomal protein mRNAs during Xenopus oocyte maturation.

    abstract::Ribosomal protein synthesis ceases upon maturation of Xenopus oocytes. We find that this cessation results from the dissociation of ribosomal protein mRNAs from polysomes and is accompanied by the deadenylation of these transcripts. A synthetic mRNA encoding ribosomal protein L1, microinjected into stage VI oocytes, i...

    journal_title:Genes & development

    pub_type: 杂志文章


    authors: Hyman LE,Wormington WM

    更新日期:1988-05-01 00:00:00