The characterization of the TFIIIA synthesized in somatic cells of Xenopus laevis.

Abstract:

:In somatic cells of Xenopus, transcription of the TFIIIA gene initiates greater than 200 bp upstream from the start site used in oocytes. The resultant mRNA encodes a protein, S-TFIIIA, that is 22 amino acids longer at its amino terminus than the abundant form of TFIIIA in oocytes (O-TFIIIA). S-TFIIIA binds the 5S RNA gene and 5S RNA, and both O- and S-TFIIIA promote the formation of stable transcription complexes on oocyte-type 5S RNA genes in an oocyte nuclear extract. We have not found any functional difference between the two forms of TFIIIA. Different transcription start sites suggest differential promoter usage--one in oocytes that permits high levels of gene activity and another that is used in somatic cells for low-level TFIIIA mRNA synthesis.

journal_name

Genes Dev

journal_title

Genes & development

authors

Kim SH,Darby MK,Joho KE,Brown DD

doi

10.1101/gad.4.9.1602

subject

Has Abstract

pub_date

1990-09-01 00:00:00

pages

1602-10

issue

9

eissn

0890-9369

issn

1549-5477

journal_volume

4

pub_type

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