Abstract：
:Endospores of Bacillus subtilis are encased in a two-layer protein shell known as the coat, which consists of a lammellar-like inner layer and an electron-dense outer layer. We report the cloning of the structural gene (designated cotE) for an alkali-soluble coat protein of 24 kD and show that the cotE gene product is a morphogenic protein required in the assembly of the outer coat. The nucleotide sequence of cotE reveals an open reading frame capable of encoding a 181-residue-long polypeptide of 21 kD. A cotE mutant was created by replacing the chromosomal gene, which was located at 145 degrees on the chromosome, with an in vitro constructed, deletion-mutated gene. The resulting cotE mutant formed normal-looking (optically refractile) spores that were heat resistant but were sensitive to lysozyme and somewhat impaired in germination. Ultrastructural analysis indicated that the mutant spores lacked the electron-dense outer layer of the coat but retained a normal-looking inner coat. The mutant spores were pleiotropically deficient in several coat proteins, including the product of cotE and the products of previously cloned cot genes A-C. Based on experiments in which expression of the cotA and cotC genes was found to be unimpaired in cotE mutant cells, we infer that the cotE gene product is involved in the assembly of the products of cotA-cotC, and certain other proteins into the electron-dense outer layer of the coat.
journal_name
Genes Devjournal_title
Genes & developmentauthors
Zheng LB,Donovan WP,Fitz-James PC,Losick Rdoi
10.1101/gad.2.8.1047subject
Has Abstractpub_date
1988-08-01 00:00:00pages
1047-54issue
8eissn
0890-9369issn
1549-5477journal_volume
2pub_type
杂志文章abstract：:Motor axons make synaptic connections with specific muscles, and this specificity unfolds during development as motoneuron growth cones make specific pathway choices and ultimately recognize and synapse on their specific muscle targets. The Drosophila clueless mutation was identified previously in a genetic screen for...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.9.23.2936
更新日期：1995-12-01 00:00:00
abstract：:Cancer stem cells (CSCs) are postulated to be a small subset of tumor cells with tumor-initiating ability that shares features with normal tissue-specific stem cells. The origin of CSCs and the mechanisms underlying their genesis are poorly understood, and it is uncertain whether it is possible to obliterate CSCs with...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.171959.111
更新日期：2011-12-15 00:00:00
abstract：:Activation of the CLB gene cluster through the assembly of Mcm1p-Fkh2p complexes at target promoters is essential for mitotic entry and transition through M phase. We show that the activation of this mitotic transcriptional program is dependent on the recruitment of Ndd1p, a coactivator that performs its essential fun...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.1074103
更新日期：2003-07-15 00:00:00
abstract：:As in many polarized cells, spindle alignment in yeast is essential and cell cycle regulated. A key step that governs spindle alignment is the selective binding of the Kar9 protein to only one of the two spindle pole bodies (SPBs). It has been suggested that cyclin-dependent kinase Cdc28, in complex with cyclin Clb4, ...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.298704
更新日期：2004-07-15 00:00:00
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journal_title：Genes & development
pub_type： 杂志文章
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更新日期：2006-06-15 00:00:00
abstract：:The recombination-activating gene 1 (RAG1) and RAG2 proteins initiate V(D)J recombination, the process that assembles the B- and T-lymphocyte antigen receptor genes of jawed vertebrates. RAG1 and RAG2 are thought to have arisen from a transposable element, but the origins of this element are not understood. We show th...
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pub_type： 杂志文章
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更新日期：2016-04-15 00:00:00
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pub_type： 杂志文章
doi：10.1101/gad.173922.111
更新日期：2011-11-01 00:00:00
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journal_title：Genes & development
pub_type： 杂志文章,评审
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更新日期：2004-08-15 00:00:00
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journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.6.11.2201
更新日期：1992-11-01 00:00:00
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journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.6.7.1332
更新日期：1992-07-01 00:00:00
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journal_title：Genes & development
pub_type： 杂志文章,评审
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更新日期：2020-04-01 00:00:00
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journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.13.9.1079
更新日期：1999-05-01 00:00:00
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journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.9.5.612
更新日期：1995-03-01 00:00:00
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journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.12.19.3096
更新日期：1998-10-01 00:00:00
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journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.279003
更新日期：2003-12-15 00:00:00
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journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.11.2.167
更新日期：1997-01-15 00:00:00
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journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.1440606
更新日期：2006-07-15 00:00:00
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journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.284828.116
更新日期：2016-08-15 00:00:00
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journal_title：Genes & development
pub_type： 杂志文章
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更新日期：2015-03-01 00:00:00
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journal_title：Genes & development
pub_type： 评论,杂志文章
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更新日期：2011-05-15 00:00:00
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journal_title：Genes & development
pub_type： 杂志文章
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更新日期：2013-03-01 00:00:00
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pub_type： 杂志文章
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更新日期：2003-09-01 00:00:00
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journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.2.9.1115
更新日期：1988-09-01 00:00:00
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journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.13.1.64
更新日期：1999-01-01 00:00:00
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journal_title：Genes & development
pub_type： 杂志文章,评审
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更新日期：2014-12-01 00:00:00
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journal_title：Genes & development
pub_type： 杂志文章
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更新日期：2001-07-01 00:00:00
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journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.2.12b.1800
更新日期：1988-12-01 00:00:00
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journal_title：Genes & development
pub_type： 杂志文章,评审
doi：10.1101/gad.1743009
更新日期：2009-02-15 00:00:00
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journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.10.18.2314
更新日期：1996-09-15 00:00:00
abstract：:The Wnt gene family encodes a group of cysteine-rich proteins implicated in intercellular signaling during several stages of vertebrate development. This family includes Wnt-1 and Wnt-3, both discovered as activated oncogenes in mouse mammary tumors. Here we describe the molecular cloning of an additional member of th...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.5.3.381
更新日期：1991-03-01 00:00:00
