Abstract：
:The P family of transposable elements in Drosophila transpose by a cut-and-paste mechanism involving double-strand gap repair. We report here that a Drosophila mutagen-sensitive mutant, mus3O9, contains a mutation in IRBP (inverted repeat binding protein), the Drosophila homolog of the mammalian Ku p70 gene. We show that the repair of double-strand DNA breaks after P-element excision is severely reduced in mus3O9 mutants using an in vivo assay for P-element transposase activity. In addition, excision products recovered from mus3O9 mutant embryos by use of a plasmid-based P-element mobility assay contain large deletions, suggesting that IRBP is involved in the repair of double-strand DNA breaks. Our findings provide the first demonstration that a mutation in the IRBP gene affects double-strand DNA break repair and suggest that DNA repair functions are conserved between Drosophila and mammals.
journal_name
Genes Devjournal_title
Genes & developmentauthors
Beall EL,Rio DCdoi
10.1101/gad.10.8.921subject
Has Abstractpub_date
1996-04-15 00:00:00pages
921-33issue
8eissn
0890-9369issn
1549-5477journal_volume
10pub_type
杂志文章abstract：:MicroRNAs (miRNAs) are approximately 22-nucleotide RNAs that are processed from characteristic precursor hairpins and pair to sites in messages of protein-coding genes to direct post-transcriptional repression. Here, we report that the miRNA iab-4 locus in the Drosophila Hox cluster is transcribed convergently from bo...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.1613108
更新日期：2008-01-01 00:00:00
abstract：:Mammalian cells mostly rely on extracellular molecules to transfer signals to other cells. However, in stress conditions, more robust mechanisms might be necessary to facilitate cell-cell communications. Cellular senescence, a stress response associated with permanent exit from the cell cycle and the development of an...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.259341.115
更新日期：2015-04-15 00:00:00
abstract：:Previous characterization of the Saccharomyces cerevisiae Spt4, Spt5, and Spt6 proteins suggested that these proteins act as transcription factors that modify chromatin structure. In this work, we report new genetic and biochemical studies of Spt4, Spt5, and Spt6 that reveal a role for these factors in transcription e...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.12.3.357
更新日期：1998-02-01 00:00:00
abstract：:Small cell lung cancer (SCLC) is an aggressive neuroendocrine cancer characterized by initial chemosensitivity followed by emergence of chemoresistant disease. To study roles for MYCN amplification in SCLC progression and chemoresistance, we developed a genetically engineered mouse model of MYCN-overexpressing SCLC. I...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.340133.120
更新日期：2020-09-01 00:00:00
abstract：:The embryonic ectoderm in Drosophila displays a highly organized arrangement of specific structures along the dorsal-ventral axis. To establish this characteristic design, cells must receive instructive cues regarding their position. We present evidence that during stages 8-9 of embryonic development, the Drosophila E...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.7.10.1937
更新日期：1993-10-01 00:00:00
abstract：:For all newborn mammals, mother's milk is the perfect nourishment, crucial for their postnatal development. Here we report that, unexpectedly, maternal western diet consumption in mice causes the production of toxic milk that contains excessive long chain and saturated fatty acids, which triggers ceramide accumulation...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.191031.112
更新日期：2012-06-15 00:00:00
abstract：:The Bloom's syndrome gene (BLM) plays a pivotal role in the maintenance of genomic stability in somatic cells. It encodes a DNA helicase (BLM) of the RecQ family, but the exact function of BLM remains elusive. To study this question, we have cloned the BLM homolog of the frog Xenopus laevis (xBLM) and have raised anti...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.822400
更新日期：2000-10-15 00:00:00
abstract：:Human TFIIA activity is composed of three subunits (alpha, beta, gamma). Here we report the isolation of a human cDNA clone encoding the gamma-subunit and the reconstitution of TFIIA activity from recombinant polypeptides (holo-TFIIA). Protein-protein interaction analysis established that the beta and gamma subunits o...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.8.19.2336
更新日期：1994-10-01 00:00:00
abstract：:We investigated the regulation of the activin/nodal-inducible distal element (DE) of the Xenopus goosecoid (gsc) promoter. On the basis of its interaction with the DE, we isolated a Xenopus homolog of the human Williams-Beuren syndrome critical region 11 (XWBSCR11), and further, show that it interacts with pathway-spe...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.963802
更新日期：2002-04-01 00:00:00
abstract：:The yeast HO gene is transcribed transiently during G1 as cells undergo START. START-specific HO activation requires two proteins, SWI4 and SWI6, which act via a motif (CACGA4) repeated up to 10 times within the URS2 region of the HO promoter. We identified a DNA-binding activity containing SWI4 and SWI6 that recogniz...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.5.11.2000
更新日期：1991-11-01 00:00:00
abstract：:We characterized a gene, extradenticle, which seems to interact with a specific subset of Drosophila homeo domain proteins, possibly affecting their target specificity. This interpretation is based on an examination of the zygotic and maternal effect phenotypes of extradenticle mutations. In embryos with reduced level...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.4.7.1209
更新日期：1990-07-01 00:00:00
abstract：:A transgenic mouse model for retinoblastoma was produced previously by directing SV40 T antigen expression to retinal photoreceptor cells using the promoter of the interstitial retinol-binding protein (IRBP) gene. This gene becomes active prior to the terminal differentiation of photoreceptors. Because T antigen-trans...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.8.11.1300
更新日期：1994-06-01 00:00:00
abstract：:Although progesterone has been recognized as essential for the establishment and maintenance of pregnancy, this steroid hormone has been recently implicated to have a functional role in a number of other reproductive events. The physiological effects of progesterone are mediated by the progesterone receptor (PR), a me...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.9.18.2266
更新日期：1995-09-15 00:00:00
abstract：:Telomerase is a ribonucleoprotein enzyme that adds telomeric repeats to chromosomes, maintaining telomere length and stabilizing chromosome ends. In vitro, telomerase from the ciliate Tetrahymena elongates single-stranded, guanosine-rich DNA primers by adding repeats of the Tetrahymena telomeric sequence, dT2G4. We ha...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.7.7b.1364
更新日期：1993-07-01 00:00:00
abstract：:Multiple DNA repair pathways maintain genome stability and ensure that DNA remains essentially unchanged over the life of a cell. Various human diseases occur if DNA repair is compromised, and most of these impact the nervous system, in some cases exclusively. However, it is often unclear what specific endogenous dama...
journal_title：Genes & development
pub_type： 杂志文章,评审
doi：10.1101/gad.301325.117
更新日期：2017-06-15 00:00:00
abstract：:The v-erbA oncogene of avian erythroblastosis virus (AEV) encodes a ligand-independent mutated version of the chicken c-erbA alpha-encoded thyroid hormone receptor. The v-erbA gene product, a 75-kD gag/v-erbA fusion protein, is phosphorylated on Ser-16/17 of its v-erbA-encoded domain, and phosphorylation at this site ...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.4.10.1663
更新日期：1990-10-01 00:00:00
abstract：:In Saccharomyces cerevisiae, the genes encoding the HO endonuclease, G1-specific cyclins CLN1 and CLN2, as well as most proteins involved in DNA synthesis, are periodically transcribed with maximal levels reached in late G1. For HO and the DNA replication genes, cell cycle stage-specific expression has been shown to b...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.6.4.557
更新日期：1992-04-01 00:00:00
abstract：:Covalent modifications of the histone N tails play important roles in eukaryotic gene expression. Histone acetylation, in particular, is required for the activation of a subset of eukaryotic genes through the targeted recruitment of histone acetyltransferases. We have reported that a histone C tail modification, ubiqu...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.1149604
更新日期：2004-01-15 00:00:00
abstract：:The association of transcription corepressors SMRT and N-CoR with retinoid and thyroid receptors results in suppression of basal transcriptional activity. A key event in nuclear receptor signaling is the hormone-dependent release of corepressor and the recruitment of coactivator. Biochemical and structural studies hav...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.13.24.3209
更新日期：1999-12-15 00:00:00
abstract：:Serotonin is a bioamine regulating bone mass accrual differently depending on its site of synthesis. It decreases accrual when synthesized in the gut, and increases it when synthesized in the brain. The signal transduction events elicited by gut-derived serotonin once it binds to the Htr1b receptor present on osteobla...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.1977210
更新日期：2010-10-15 00:00:00
abstract：:Cis-regulatory modules (CRMs) are defined by unique combinations of transcription factor-binding sites. Emerging evidence suggests that the number, affinity, and organization of sites play important roles in regulating enhancer output and, ultimately, gene expression. Here, we investigate how the cis-regulatory logic ...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.307132.117
更新日期：2018-03-01 00:00:00
abstract：:RNA polymerase II nascent transcripts are capped during pausing before elongation. Here we report that hSPT5, the human homolog of yeast elongation factor SPT5, interacts directly with the capping enzyme. hSPT5 stimulated capping enzyme guanylylation and mRNA capping by severalfold. Although RNA 5'-triphosphatase acti...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.13.14.1774
更新日期：1999-07-15 00:00:00
abstract：:TDP-43 is a critical RNA-binding factor associated with pre-mRNA splicing in mammals. Its expression is tightly autoregulated, with loss of this regulation implicated in human neuropathology. We demonstrate that TDP-43 overexpression in humans and mice activates a 3' untranslated region (UTR) intron, resulting in exci...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.194829.112
更新日期：2012-08-01 00:00:00
abstract：:Binding of microRNA (miRNA) to mRNA within the RNA-induced silencing complex (RISC) leads to either translational inhibition or to destruction of the target mRNA. Both of these functions are executed by Argonaute 2 (Ago2). Using hematopoiesis in mice as a model system to study the physiological function of Ago2 in viv...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.1565607
更新日期：2007-08-15 00:00:00
abstract：:The 134-bp enhancer region of the pancreatic elastase I gene is sufficient to direct pancreatic acinar cell-specific transcription in transgenic mice and in transfected cells in culture. Ten-base-pair scanner mutations in three separate enhancer domains that inactivate enhancer function in transfected pancreatic cells...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.3.5.687
更新日期：1989-05-01 00:00:00
abstract：:Noncoding RNA regulators have been implicated in almost all imaginable cellular processes. Here we review how regulatory small RNAs such as Spot42, SgrS, GlmY, and GlmZ and a cis-encoded ribozyme in glmS mRNA control sugar metabolism. Besides discussing the physiological implications, we show how the study of these mo...
journal_title：Genes & development
pub_type： 杂志文章,评审
doi：10.1101/gad.1717808
更新日期：2008-11-01 00:00:00
abstract：:The integration of viral DNA into the host genome is an essential step in the retrovirus life cycle. To understand this process better, we have examined the native state of viral DNA in cells acutely infected by murine leukemia virus (MLV), using both a physical assay for viral DNA and a functional assay for integrati...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.3.4.469
更新日期：1989-04-01 00:00:00
abstract：:Pre-mRNA processing is coupled with transcription. It is still unclear if the transcription machinery can also directly affect the cytoplasmic fate of a transcript, such as its intracellular localization. In yeast, the RNA-binding protein She2p binds several mRNAs and targets them for localization at the bud. Here we ...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.1937510
更新日期：2010-09-01 00:00:00
abstract：:One of the central requirements for eukaryotic chromosome stability is the maintenance of the simple sequence tracts at telomeres. In this study, we use genetic and physical assays to reveal the nature of a novel mechanism by which telomere length is controlled. This mechanism, telomeric rapid deletion (TRD), is capab...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.10.11.1310
更新日期：1996-06-01 00:00:00
abstract：:The product of the zygotically active decapentaplegic (dpp) gene appears to function as a morphogen that specifies positional information in the dorsal half of the Drosophila embryo. The dorsal-specific transcription of dpp is the key step in establishing a morphogen gradient. We demonstrate here that multiple regions...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.7.4.694
更新日期：1993-04-01 00:00:00
