Abstract:
:Noncoding RNA regulators have been implicated in almost all imaginable cellular processes. Here we review how regulatory small RNAs such as Spot42, SgrS, GlmY, and GlmZ and a cis-encoded ribozyme in glmS mRNA control sugar metabolism. Besides discussing the physiological implications, we show how the study of these molecules contributed to our understanding of the mechanisms and of general principles of RNA-based regulation. These include the post-transcriptional repression or activation of gene expression within polycistronic mRNAs; novel ribonucleoprotein complexes composed of small RNA, Hfq, and/or RNase E; and the hierarchical action of regulatory RNAs.
journal_name
Genes Devjournal_title
Genes & developmentauthors
Görke B,Vogel Jdoi
10.1101/gad.1717808subject
Has Abstractpub_date
2008-11-01 00:00:00pages
2914-25issue
21eissn
0890-9369issn
1549-5477pii
22/21/2914journal_volume
22pub_type
杂志文章,评审abstract::The ETS transcription factors perform distinct biological functions despite conserving a highly similar DNA-binding domain. One distinguishing property of a subset of ETS proteins is a conserved region of 80 amino acids termed the Pointed (PNT) domain. Using enzyme kinetics we determined that the Ets-1 PNT domain cont...
journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.950902
更新日期:2002-01-01 00:00:00
abstract::The YAP transcription coactivator has been implicated as an oncogene and is amplified in human cancers. Recent studies have established that YAP is phosphorylated and inhibited by the Hippo tumor suppressor pathway. Here we demonstrate that the TEAD family transcription factors are essential in mediating YAP-dependent...
journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.1664408
更新日期:2008-07-15 00:00:00
abstract::Disruption of the balanced modulation of reversible tyrosine phosphorylation has been implicated in the etiology of various human cancers, including breast cancer. Protein Tyrosine Phosphatase N23 (PTPN23) resides in chromosomal region 3p21.3, which is hemizygously or homozygously lost in some breast cancer patients. ...
journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.304261.117
更新日期:2017-10-01 00:00:00
abstract::RBP is a cellular protein that functions as a transcriptional repressor in mammalian cells. RBP has elicited great interest lately because of its established roles in regulating gene expression, in Drosophila and mouse development, and as a component of the Notch signal transduction pathway. This report focuses on the...
journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.12.11.1621
更新日期:1998-06-01 00:00:00
abstract::P-element transformants of a single rRNA gene (rDNA) were used to investigate the relationship between the organization of the nucleolus organizer (NO) and rDNA function in Drosophila melanogaster. In situ hybridization to rRNA in polytene nuclei of salivary glands demonstrated that an rRNA gene can be transcribed at ...
journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.2.12b.1745
更新日期:1988-12-01 00:00:00
abstract::Many bacterial small regulatory RNAs (sRNAs) pair with mRNA targets, stimulating or inhibiting mRNA stability and/or translation. Regulation of these sRNAs is usually due to tight transcriptional regulation of synthesis.In this issue of Genes & Development and a related paper in Molecular Microbiology, Figueroa-Bossi ...
journal_title:Genes & development
pub_type: 评论,杂志文章
doi:10.1101/gad.1846609
更新日期:2009-09-01 00:00:00
abstract::The Hox-2 locus on chromosome 11 represents one of the major clusters of homeo-box-containing genes in the mouse. We have identified two new members (Hox-2.6 and Hox-2.7), which form part of this cluster of seven linked genes, and it appears that the Hox-2 locus is related by duplication and divergence to at least one...
journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.2.11.1424
更新日期:1988-11-01 00:00:00
abstract::Endospores of Bacillus subtilis are encased in a two-layer protein shell known as the coat, which consists of a lammellar-like inner layer and an electron-dense outer layer. We report the cloning of the structural gene (designated cotE) for an alkali-soluble coat protein of 24 kD and show that the cotE gene product is...
journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.2.8.1047
更新日期:1988-08-01 00:00:00
abstract::Expression of both N-myc and c-myc is induced rapidly and dramatically in normal pre-B cells after stimulation with interleukin-7 (IL-7), a pre-B cell-specific growth factor. These IL-7-induced increases in N-myc and c-myc expression are mediated at the transcriptional level; for N-myc, a major portion of the inductio...
journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.6.1.61
更新日期:1992-01-01 00:00:00
abstract::Nuclear pore complexes (NPCs) emerged as nuclear transport channels in eukaryotic cells ∼1.5 billion years ago. While the primary role of NPCs is to regulate nucleo-cytoplasmic transport, recent research suggests that certain NPC proteins have additionally acquired the role of affecting gene expression at the nuclear ...
journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.280941.116
更新日期:2016-05-15 00:00:00
abstract::DNA replication remains unfinished in many Drosophila polyploid cells, which harbor disproportionately fewer copies of late-replicating chromosomal regions. By analyzing paired-end high-throughput sequence data from polytene larval salivary gland cells, we define 112 underreplicated (UR) euchromatic regions 60-480 kb ...
journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.245811.114
更新日期:2014-08-15 00:00:00
abstract::In the cytoplasm of oocytes and early embryos, addition of poly(A) to mRNAs can activate their translation. We demonstrate that despite many differences between poly(A) addition in the cytoplasm and nucleus, these two forms of polyadenylation may involve identical trans-acting factors. Nuclear polyadenylation requires...
journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.8.9.1106
更新日期:1994-05-01 00:00:00
abstract::The enzyme telomerase, which elongates chromosome termini, is a critical factor in determining long-term cellular proliferation and tissue renewal. Hence, even small differences in telomerase levels can have substantial consequences for human health. In budding yeast, telomerase consists of the catalytic Est2 protein ...
journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.246256.114
更新日期:2014-10-01 00:00:00
abstract::Eukaryotic morphogenesis is seeded with the establishment and subsequent amplification of polarity cues at key times during the cell cycle, often using (cyclic) nucleotide signals. We discovered that flagellum de- and repolarization in the model prokaryote Caulobacter crescentus is precisely orchestrated through at le...
journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.222679.113
更新日期:2013-09-15 00:00:00
abstract::Transcription factor IIA has been shown to interact with the TATA-binding protein and to act early during preinitiation complex formation. The human factor is composed of three subunits (alpha, beta, gamma). A human cDNA clone encoding the largest subunit of TFIIA (alpha) was isolated. The recombinant alpha polypeptid...
journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.7.11.2246
更新日期:1993-11-01 00:00:00
abstract::A Drosophila FGF receptor homolog (DFGF-R2/DFRI) termed Heartless (Htl) is expressed in the embryonic mesoderm. The phenotypes of null mutant embryos demonstrated that Htl is a central player that is required for the development of several mesodermal lineages. No abnormalities in the primary specification of the mesod...
journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.10.23.2993
更新日期:1996-12-01 00:00:00
abstract::Sox10 is a high-mobility-group transcriptional regulator in early neural crest. Without Sox10, no glia develop throughout the peripheral nervous system. Here we show that Sox10 is restricted in the central nervous system to myelin-forming oligodendroglia. In Sox10-deficient mice progenitors develop, but terminal diffe...
journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.215802
更新日期:2002-01-15 00:00:00
abstract::In Xenopus embryos, a dorsal-ventral patterning gradient is generated by diffusing Chordin/bone morphogenetic protein (BMP) complexes cleaved by BMP1/Tolloid metalloproteinases in the ventral side. We developed a new BMP1/Tolloid assay using a fluorogenic Chordin peptide substrate and identified an unexpected negative...
journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.1839309
更新日期:2009-11-01 00:00:00
abstract::The ability to maintain and expand the pool of adipocytes in adults is integral to the regulation of energy balance, tissue/stem cell homeostasis, and disease pathogenesis. For decades, our knowledge of adipocyte precursors has relied on cellular models. The identity of native adipocyte precursors has remained unclear...
journal_title:Genes & development
pub_type: 杂志文章,评审
doi:10.1101/gad.293704.116
更新日期:2017-01-15 00:00:00
abstract::Mice carrying a germ-line null mutation of the prolactin receptor gene have been produced by gene targeting in embryonic stem cells. Heterozygous females showed almost complete failure of lactation attributable to greatly reduced mammary gland development after their first, but not subsequent, pregnancies. Homozygous ...
journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.11.2.167
更新日期:1997-01-15 00:00:00
abstract::Directed cell motility is at the basis of biological phenomena such as development, wound healing, and metastasis. It has been shown that substrate attachments mediate motility by coupling the cell's cytoskeleton with force generation. However, it has been unclear how the persistence of cell directionality is facilita...
journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.190413.112
更新日期:2012-09-01 00:00:00
abstract::Accurate and regulated transcription by RNA polymerase II requires the assembly of an initiation complex involving multiple protein-DNA and protein-protein interactions. A key event is binding of TFIID, a complex consisting of TBP and associated factors (TAFs) to the template DNA. The TAF subunits of TFIID carry out d...
journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.9.22.2747
更新日期:1995-11-15 00:00:00
abstract::The Drosophila external sensory organ forms in a lineage elaborating from a single precursor cell via a stereotypical series of asymmetric divisions. HAMLET transcription factor expression demarcates the lineage branch that generates two internal cell types, the external sensory neuron and thecogen. In HAMLET mutant o...
journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.1170904
更新日期:2004-03-15 00:00:00
abstract::Hundreds of microRNAs (miRNAs) have been discovered in metazoans and plants, and understanding of their biogenesis has advanced dramatically; however, relatively little is known about the cofactors necessary for miRNA regulation of target gene expression. In Caenorhabditis elegans, the conserved miRNA let-7 and its pa...
journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.17153811
更新日期:2011-10-01 00:00:00
abstract::The histone variants H3.3 and H2A.Z have recently emerged as two of the most important features in transcriptional regulation, the molecular mechanism of which still remains poorly understood. In this study, we investigated the regulation of H3.3 and H2A.Z on chromatin dynamics during transcriptional activation. Our i...
journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.222174.113
更新日期:2013-10-01 00:00:00
abstract::Fgf-10-deficient mice (Fgf-10(-/-)) were generated to determine the role(s) of Fgf-10 in vertebrate development. Limb bud initiation was abolished in Fgf-10(-/-) mice. Strikingly, Fgf-10(-/-) fetuses continued to develop until birth, despite the complete absence of both fore- and hindlimbs. Fgf-10 is necessary for api...
journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.12.20.3156
更新日期:1998-10-15 00:00:00
abstract::Medulloblastomas are among the most common malignancies in childhood, and they are associated with substantial mortality and morbidity. The molecular pathogenesis as well as the ontogeny of these neoplasms is still poorly understood. We have generated a mouse model for medulloblastoma by Cre-LoxP-mediated inactivation...
journal_title:Genes & development
pub_type: 杂志文章
doi:
更新日期:2000-04-15 00:00:00
abstract::Ecsit is a cytosolic adaptor protein essential for inflammatory response and embryonic development via the Toll-like and BMP (bone morphogenetic protein) signal transduction pathways, respectively. Here, we demonstrate a mitochondrial function for Ecsit (an evolutionary conserved signaling intermediate in Toll pathway...
journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.408407
更新日期:2007-03-01 00:00:00
abstract::The mechanisms that determine the relative positions of floral organs, and thereby their numbers, is a poorly understood aspect of flower development. We isolated a petunia mutant, floozy (fzy), in which the formation of floral organ primordia in the outermost three floral whorls and one of the two bracts at the base ...
journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.219502
更新日期:2002-03-15 00:00:00
abstract::We describe three strains of transgenic mice derived by embryo microinjection of DNA consisting of a long terminal repeat (LTR) of Moloney murine sarcoma virus (Mo-MSV) linked to the murine c-mos coding sequences. Southern analysis of the genomic DNA of these strains suggested that in each case the transgene had integ...
journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.1.10.1327
更新日期:1987-12-01 00:00:00