Abstract:
:Human TFIIA activity is composed of three subunits (alpha, beta, gamma). Here we report the isolation of a human cDNA clone encoding the gamma-subunit and the reconstitution of TFIIA activity from recombinant polypeptides (holo-TFIIA). Protein-protein interaction analysis established that the beta and gamma subunits of TFIIA interact with the TBP component of TFIID. The alpha-subunit is recruited into the complex by association with the gamma-subunit. Functional studies indicate that recombinant TFIIA stimulates basal TFIID-dependent transcription but is without effect on TBP-dependent transcription. Our studies indicate that TFIIA not only functions by physically removing negative components present in TFIID (antirepression), as demonstrated previously, but that it can stimulate basal transcription through components of the TFIID complex. Holo-TFIIA also stimulated activation of transcription in vitro as well as in vivo in transfected HeLa cells.
journal_name
Genes Devjournal_title
Genes & developmentauthors
Sun X,Ma D,Sheldon M,Yeung K,Reinberg Ddoi
10.1101/gad.8.19.2336subject
Has Abstractpub_date
1994-10-01 00:00:00pages
2336-48issue
19eissn
0890-9369issn
1549-5477journal_volume
8pub_type
杂志文章abstract::The Rbfox proteins (Rbfox1, Rbfox2, and Rbfox3) regulate the alternative splicing of many important neuronal transcripts and have been implicated in a variety of neurological disorders. However, their roles in brain development and function are not well understood, in part due to redundancy in their activities. Here w...
journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.182477.111
更新日期:2012-03-01 00:00:00
abstract::S-phase cyclin-dependent kinase Cdc28-Clb5 (CDK-S) and Dbf4-dependent kinase Cdc7-Dbf4 (DDK) are highly conserved kinases well known for their roles in the initiation of DNA replication. CDK-S is also essential for initiation of meiotic recombination because it phosphorylates Ser30 of Mer2, a meiosis-specific double-s...
journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.1626408
更新日期:2008-02-01 00:00:00
abstract::The product of the nuclear MRS2 gene, Mrs2p, is the only candidate splicing factor essential for all group II introns in mitochondria of the yeast Saccharomyces cerevisiae. It has been shown to be an integral protein of the inner mitochondrial membrane, structurally and functionally related to the bacterial CorA Mg(2+...
journal_title:Genes & development
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journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.1742908
更新日期:2008-12-15 00:00:00
abstract::All transcription factors are equal, but some are more equal than others. In the 25 yr since the gene encoding the microphthalmia-associated transcription factor (MITF) was first isolated, MITF has emerged as a key coordinator of many aspects of melanocyte and melanoma biology. Like all transcription factors, MITF bin...
journal_title:Genes & development
pub_type: 杂志文章,评审
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abstract::Small noncoding RNAs, including small interfering RNAs (siRNAs) and micro RNAs (miRNAs) of approximately 21 nucleotides (nt) in length, have emerged as potent regulators of gene expression at both transcriptional and post-transcriptional levels in diverse organisms. Here we report the identification of a novel class o...
journal_title:Genes & development
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abstract::The subdivision of the Drosophila body into distinct terminal and central domains depends on the torso (tor) protein, a putative receptor tyrosine kinase that is active at both ends of the early embryo. We show that the tor protein is uniformly expressed along the surface membrane of early embryos despite its localize...
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pub_type: 杂志文章
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journal_title:Genes & development
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journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.6.12a.2382
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abstract::Tuberous sclerosis (TSC) is an autosomal dominant disease characterized by hamartoma formation in various organs and is caused by mutations targeting either the TSC1 or TSC2 genes. TSC1 and TSC2 proteins form a functionally interdependent dimeric complex. Phosphorylation of either TSC subunit by different kinases regu...
journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.1624008
更新日期:2008-04-01 00:00:00
abstract::Cell for cell, probably no human cancer is as aggressive as melanoma. It is among a handful of cancers whose dimensions are reported in millimeters. Tumor thickness approaching 4 mm presents a high risk of metastasis, and a diagnosis of metastatic melanoma carries with it an abysmal median survival of 6-9 mo. What fea...
journal_title:Genes & development
pub_type: 杂志文章,评审
doi:10.1101/gad.1437206
更新日期:2006-08-15 00:00:00
abstract::Motor axons make synaptic connections with specific muscles, and this specificity unfolds during development as motoneuron growth cones make specific pathway choices and ultimately recognize and synapse on their specific muscle targets. The Drosophila clueless mutation was identified previously in a genetic screen for...
journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.9.23.2936
更新日期:1995-12-01 00:00:00
abstract::Sensory organ formation in Drosophila is activated by proneural genes that encode basic-helix-loop-helix (bHLH) transcription factors. These genes are antagonized by hairy and other proline-bHLH proteins. hairy has not been shown to bind to DNA and has been proposed to form inactive heterodimers with proneural activat...
journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.8.22.2743
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journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.223902
更新日期:2002-04-15 00:00:00
abstract::The cytokine-activated Janus kinase (JAK)/signal transducer and activator of transcription (STAT) pathway plays an important role in the control of a wide variety of biological processes. When misregulated, JAK/STAT signaling is associated with various human diseases, such as immune disorders and tumorigenesis. To gai...
journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.1320705
更新日期:2005-08-15 00:00:00
abstract::Oxidative tissues such as heart undergo a dramatic perinatal mitochondrial biogenesis to meet the high-energy demands after birth. PPARgamma coactivator-1 (PGC-1) alpha and beta have been implicated in the transcriptional control of cellular energy metabolism. Mice with combined deficiency of PGC-1alpha and PGC-1beta ...
journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.1661708
更新日期:2008-07-15 00:00:00
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journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.227454.113
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abstract::Somitogenesis involves the segmentation of the paraxial mesoderm into units along the anteroposterior axis. Here we show a role for Eph and ephrin signaling in the patterning of presomitic mesoderm and formation of the somites. Ephrin-A-L1 and ephrin-B2 are expressed in an iterative manner in the developing somites an...
journal_title:Genes & development
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更新日期:1998-10-01 00:00:00
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journal_title:Genes & development
pub_type: 杂志文章
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更新日期:2005-02-15 00:00:00
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journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.3.8.1190
更新日期:1989-08-01 00:00:00
abstract::Many small nucleolar RNAs (snoRNAs) in vertebrates are encoded within introns of protein genes. We have reported previously that two isoforms of human U17 snoRNA are encoded in introns of the cell-cycle regulatory gene, RCC1. We have now investigated the mechanism of processing of U17 RNAs and of another intron-encode...
journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.9.11.1411
更新日期:1995-06-01 00:00:00
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journal_title:Genes & development
pub_type: 杂志文章
doi:
更新日期:2000-04-15 00:00:00
abstract::Mismatch repair (MMR) has important roles in meiotic and mitotic recombination, DNA damage signaling, and various aspects of DNA metabolism including class-switch recombination, somatic hypermutation, and triplet-repeat expansion. Defects in MMR are responsible for human cancers characterized by microsatellite instabi...
journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.430107
更新日期:2007-09-01 00:00:00
abstract::Interruption of translation in Escherichia coli can lead to the addition of an 11-residue carboxy-terminal peptide tail to the nascent chain. This modification is mediated by SsrA RNA (also called 10Sa RNA and tmRNA) and marks the tagged polypeptide for proteolysis. Degradation in vivo of lambda repressor amino-termin...
journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.12.9.1338
更新日期:1998-05-01 00:00:00
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journal_title:Genes & development
pub_type: 杂志文章
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更新日期:2012-04-01 00:00:00
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journal_title:Genes & development
pub_type: 杂志文章
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更新日期:2007-05-15 00:00:00
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journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.3.7.1062
更新日期:1989-07-01 00:00:00
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journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.2.11.1400
更新日期:1988-11-01 00:00:00