Abstract:
:The Ultrabithorax (Ubx) gene of Drosophila melanogaster encodes a family of UBX proteins that are thought to specify the developmental fates of segments in the posterior thorax and anterior abdomen by controlling the expression of a set of target genes. UBX proteins bind DNA in vitro, and they activate or repress different natural and synthetic target promoters in cultured cells. Here it is shown that a purified UBX protein can stimulate transcription of a synthetic target gene in extracts of cultured D. melanogaster cells. Stimulation is dependent on the presence of upstream, promoter-region binding sites but is independent of binding site orientation. A naturally occurring binding site cluster and a binding site consensus sequence consisting of TAA trinucleotide repeats can mediate this activation. A minimal promoter fused to such sites is activated by UBX, suggesting that transcriptional stimulation could result from an interaction between the promoter-bound protein and the general transcriptional machinery.
journal_name
Genes Devjournal_title
Genes & developmentauthors
Johnson FB,Krasnow MAdoi
10.1101/gad.4.6.1044subject
Has Abstractpub_date
1990-06-01 00:00:00pages
1044-52issue
6eissn
0890-9369issn
1549-5477journal_volume
4pub_type
杂志文章abstract::Faithful inheritance of genetic information requires that DNA be copied only once each cell cycle. Initiation of DNA replication involves the establishment of a prereplication complex (pre-RC) and subsequent activation by CDK/cyclins, converting the pre-RC to a post-RC. The origin recognition complex (ORC), Cdc6p, and...
journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.11.24.3375
更新日期:1997-12-15 00:00:00
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journal_title:Genes & development
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doi:10.1101/gad.254631.114
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journal_title:Genes & development
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更新日期:2011-02-01 00:00:00
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journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.1388806
更新日期:2006-03-15 00:00:00
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journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.1.10.1327
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journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.862101
更新日期:2001-04-01 00:00:00
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journal_title:Genes & development
pub_type: 杂志文章
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journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.931001
更新日期:2001-12-15 00:00:00
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journal_title:Genes & development
pub_type: 杂志文章
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journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.8.8.959
更新日期:1994-04-15 00:00:00
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journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.303537.117
更新日期:2017-07-15 00:00:00
abstract::A conditional allele of type 1 protein phosphatase (glc7-129) in Saccharomyces cerevisiae causes first cycle arrest in G2/M, characterized by cells with a short spindle and high H1 kinase activity. Point-of-execution experiments indicate Glc7p function is required in G2/M just before anaphase for the completion of mit...
journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.13.5.517
更新日期:1999-03-01 00:00:00
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journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.6.1.61
更新日期:1992-01-01 00:00:00
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journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.11.6.761
更新日期:1997-03-15 00:00:00
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journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.261677.115
更新日期:2015-08-15 00:00:00
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journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.260174.115
更新日期:2015-05-01 00:00:00
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journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.12.1.45
更新日期:1998-01-01 00:00:00
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journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.231274.113
更新日期:2014-02-15 00:00:00
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journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.9.8.925
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journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.967602
更新日期:2002-03-01 00:00:00
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journal_title:Genes & development
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journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.7.3.367
更新日期:1993-03-01 00:00:00
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journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.7.2.320
更新日期:1993-02-01 00:00:00
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journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.11.22.2972
更新日期:1997-11-15 00:00:00
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journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.9.14.1797
更新日期:1995-07-15 00:00:00
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journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.7.2.180
更新日期:1993-02-01 00:00:00
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journal_title:Genes & development
pub_type: 杂志文章
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更新日期:2008-05-15 00:00:00
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journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.430807
更新日期:2007-08-15 00:00:00
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journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.2.12b.1800
更新日期:1988-12-01 00:00:00
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journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.12.21.3331
更新日期:1998-11-01 00:00:00