Abstract：
:DOCK180 is involved in integrin signaling through CrkII-p130(Cas) complexes. We have studied the involvement of DOCK180 in Rac1 signaling cascades. DOCK180 activated JNK in a manner dependent on Rac1, Cdc42Hs, and SEK, and overexpression of DOCK180 increased the amount of GTP-bound Rac1 in 293T cells. Coexpression of CrkII and p130(Cas) enhanced this DOCK180-dependent activation of Rac1. Furthermore, we observed direct binding of DOCK180 to Rac1, but not to RhoA or Cdc42Hs. Dominant-negative Rac1 suppressed DOCK180-induced membrane spreading. These results strongly suggest that DOCK180 is a novel activator of Rac1 and involved in integrin signaling.
journal_name
Genes Devjournal_title
Genes & developmentauthors
Kiyokawa E,Hashimoto Y,Kobayashi S,Sugimura H,Kurata T,Matsuda Mdoi
10.1101/gad.12.21.3331subject
Has Abstractpub_date
1998-11-01 00:00:00pages
3331-6issue
21eissn
0890-9369issn
1549-5477journal_volume
12pub_type
杂志文章abstract：:Exposure of hematopoietic progenitors to gamma-irradiation (IR) induces p53-dependent apoptosis and a p53-independent G2/M cell cycle arrest. These responses to DNA-damage can be inhibited by treatment with cytokine growth factors. Here we report that gamma-IR-induced apoptosis and cell cycle arrest are suppressed by ...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.12.8.1099
更新日期：1998-04-15 00:00:00
abstract：:Pre-rRNA transcription by RNA Polymerase I (Pol I) is very robust on active rDNA repeats. Loss of yeast Topoisomerase I (Top1) generated truncated pre-rRNA fragments, which were stabilized in strains lacking TRAMP (Trf4/Trf5-Air1/Air2-Mtr4 polyadenylation complexes) or exosome degradation activities. Loss of both Top1...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.573310
更新日期：2010-07-15 00:00:00
abstract：:Members of the metazoan nuclear receptor superfamily regulate gene expression programs in response to binding of cognate lipophilic ligands. Evolutionary studies using bioinformatics tools have concluded that lower eukaryotes, such as fungi, lack nuclear receptor homologs. Here we review recent discoveries suggesting ...
journal_title：Genes & development
pub_type： 杂志文章,评审
doi：10.1101/gad.1743009
更新日期：2009-02-15 00:00:00
abstract：:The nonsense-mediated mRNA decay (NMD) pathway selectively degrades mRNAs harboring premature termination codons (PTCs). Seven genes (smg-1-7, for suppressor with morphological effect on genitalia) that are essential for NMD were originally identified in the nematode Caenorhabditis elegans, and orthologs of these gene...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.417707
更新日期：2007-05-01 00:00:00
abstract：:The Tat protein of human immunodeficiency virus type 1 (HIV-1) activates transcription following binding to nascent trans-activation response (TAR) RNA downstream of the transcription start site. Because Tat functions when bound to RNA, and in a position-dependent manner, it has been proposed that Tat works by a novel...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.5.12b.2496
更新日期：1991-12-01 00:00:00
abstract：:Constitutive activation of the Wnt/APC/beta-catenin pathway is a frequent initiating event in gastrointestinal carcinogenesis. Mutations in the Adenomatous Polyposis Coli (APC) gene up-regulate Wnt signaling by stabilizing beta-catenin and causing activation of targets important in proliferation control. Here we show ...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.1260605
更新日期：2005-02-01 00:00:00
abstract：:A complex array of genetic factors regulates neuronal dendrite morphology. Epigenetic regulation of gene expression represents a plausible mechanism to control pathways responsible for specific dendritic arbor shapes. By studying the Drosophila dendritic arborization (da) neurons, we discovered a role of the double-br...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.239962.114
更新日期：2014-09-01 00:00:00
abstract：:Expression of the gene encoding atrial natriuretic factor (ANF) during mouse cardiac embryogenesis was studied using in situ hybridization to histologic sections. ANF mRNA was first detected in a subpopulation of myocardial cells at day 8 of embryogenesis. During day 9, abundant hybridization of the ANF probe to the a...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.1.7.693
更新日期：1987-09-01 00:00:00
abstract：:Transcriptional activation of the human U1 snRNA genes is dependent on a noncanonical octamer element contained within an upstream enhancer. The U1 octamer only weakly recruits the Oct-1 POU domain, although recruitment is stimulated by a peptide containing the Oct-1-binding domain of SNAP190. Structural analysis of t...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.1021002
更新日期：2002-11-01 00:00:00
abstract：:Transcription factor IIA has been shown to interact with the TATA-binding protein and to act early during preinitiation complex formation. The human factor is composed of three subunits (alpha, beta, gamma). A human cDNA clone encoding the largest subunit of TFIIA (alpha) was isolated. The recombinant alpha polypeptid...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.7.11.2246
更新日期：1993-11-01 00:00:00
abstract：:The removal of the 5' cap structure by the decapping enzyme DCP2 inhibits translation and generally commits the mRNA to irreversible 5'-to-3' exonucleolytic degradation by XRN1. DCP2 catalytic activity is stimulated by DCP1, and these proteins form the conserved core of the decapping complex. Additional decapping fact...
journal_title：Genes & development
pub_type： 杂志文章,评审
doi：10.1101/gad.227843.113
更新日期：2013-12-15 00:00:00
abstract：:A cDNA library enriched for transcripts that accumulate from embryonic day 7 until day 1 posthatching was constructed from poly(A)+ RNA of chick optic lobe tissue. From this library, three recombinants were isolated that correspond to neural mRNAs appearing during the major period of synaptogenesis in the retinotectal...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.1.7.699
更新日期：1987-09-01 00:00:00
abstract：:A new gene, SCH9, was isolated from Saccharomyces cerevisiae by its ability to complement a cdc25ts mutation. Sequence analysis indicates that it encodes a 90,000-dalton protein with a carboxy-terminal domain homologous to yeast and mammalian cAMP-dependent protein kinase catalytic subunits. In addition to suppressing...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.2.5.517
更新日期：1988-05-01 00:00:00
abstract：:Mammalian telomeres are coated by the sequence-specific, DNA-binding protein, TRF1, a negative regulator of telomere length. Previous results showed that ADP-ribosylation of TRF1 by tankyrase 1 released TRF1 from telomeres and promoted telomere elongation. We now show that loss of TRF1 from telomeres results in ubiqui...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.1077103
更新日期：2003-06-01 00:00:00
abstract：:In yeast, galactose triggers a rapid GAL4-dependent induction of galactose/melibiose regulon (GAL/MEL) gene transcription, and glucose represses this activation. We discovered that alterations in the physical state of the GAL4 protein correlate with activation and repression of the GAL/MEL genes. Using Western immunob...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.3.8.1157
更新日期：1989-08-01 00:00:00
abstract：:Coliphage N4 is a double-stranded DNA virus that requires the sequential activity of three different RNA polymerases during infection. The N4 virion RNA polymerase, which is carried in the virion and is injected with the DNA at the start of infection, is responsible for the synthesis of N4 early RNAs. In vitro, the vi...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.6.10.2010
更新日期：1992-10-01 00:00:00
abstract：:FISH analysis of well-spread chromosomes reveals that homologs are paired in vegetatively growing budding yeast diploid cells, via multiple interstitial interactions, and independent of recA homologs and mating type heterozygosity. Pairing is present during G1 and G2, and in cells arrested at G1 by mating pheromone, b...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.13.12.1627
更新日期：1999-06-15 00:00:00
abstract：:The HAT4 gene from the plant Arabidopsis thaliana encodes a homeo domain protein that contains a leucine zipper motif. Homeo domain-leucine zipper (HD-Zip) proteins have not been found in animal systems, suggesting that HAT4 may define a new family of transcription factors that regulate higher plant development. To ex...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.7.3.367
更新日期：1993-03-01 00:00:00
abstract：:The cytokine-activated Janus kinase (JAK)/signal transducer and activator of transcription (STAT) pathway plays an important role in the control of a wide variety of biological processes. When misregulated, JAK/STAT signaling is associated with various human diseases, such as immune disorders and tumorigenesis. To gai...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.1320705
更新日期：2005-08-15 00:00:00
abstract：:Keratinocyte growth factor (KGF), also known as fibroblast growth factor 7 (FGF7), is synthesized by skin fibroblasts. However, its mitogenic activity is on skin keratinocytes, where it is the most potent growth factor identified thus far. To explore KGF's function in vivo, we used embryonic stem cell technology to ge...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.10.2.165
更新日期：1996-01-15 00:00:00
abstract：:Although Apc is well characterized as a tumor-suppressor gene in the intestine, the precise mechanism of this suppression remains to be defined. Using a novel inducible Ahcre transgenic line in conjunction with a loxP-flanked Apc allele we, show that loss of Apc acutely activates Wnt signaling through the nuclear accu...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.287404
更新日期：2004-06-15 00:00:00
abstract：:The number of known long noncoding RNA (lncRNA) functions is rapidly growing, but how those functions are encoded in their sequence and structure remains poorly understood. NORAD (noncoding RNA activated by DNA damage) is a recently characterized, abundant, and highly conserved lncRNA that is required for proper mitot...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.309138.117
更新日期：2018-01-01 00:00:00
abstract：:PBAF and BAF are two chromatin-remodeling complexes of the SWI/SNF family essential for mammalian transcription and development. Although these complexes share eight identical subunits, only PBAF can facilitate transcriptional activation by nuclear receptors in vitro. Here we show that these complexes have selectivity...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.1323805
更新日期：2005-07-15 00:00:00
abstract：:Hypoxia induces rapid and dramatic changes in cellular metabolism, in part through inhibition of target of rapamycin (TOR) kinase complex 1 (TORC1) activity. Genetic studies have shown the tuberous sclerosis tumor suppressors TSC1/2 and the REDD1 protein to be essential for hypoxia regulation of TORC1 activity in Dros...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.1617608
更新日期：2008-01-15 00:00:00
abstract：:Chromatin reorganization is essential for transcriptional control by sequence-specific transcription factors. However, the molecular link between transcriptional control and chromatin reconfiguration remains unclear. By colocalization of the nuclear ecdysone receptor (EcR) on the ecdysone-induced puff in the salivary ...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.1857410
更新日期：2010-01-15 00:00:00
abstract：:The ability to maintain and expand the pool of adipocytes in adults is integral to the regulation of energy balance, tissue/stem cell homeostasis, and disease pathogenesis. For decades, our knowledge of adipocyte precursors has relied on cellular models. The identity of native adipocyte precursors has remained unclear...
journal_title：Genes & development
pub_type： 杂志文章,评审
doi：10.1101/gad.293704.116
更新日期：2017-01-15 00:00:00
abstract：:The subdivision of the lateral mesoderm into a visceral (splanchnic) and a somatic layer is a crucial event during early mesoderm development in both arthropod and vertebrate embryos. In Drosophila, this subdivision leads to the differential development of gut musculature versus body wall musculature. Here we report t...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.917101
更新日期：2001-11-01 00:00:00
abstract：:U4 and U6 small nuclear RNAs are associated by an extensive base-pairing interaction that must be disrupted and reformed with each round of splicing. U4 mutations within the U4/U6 interaction domain destabilize the complex in vitro and cause a cold-sensitive phenotype in vivo. Restabilization of the U4/U6 helix by dom...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.5.5.773
更新日期：1991-05-01 00:00:00
abstract：:The mammalian high mobility group proteins HMG1 and HMG2 are abundant, chromatin-associated proteins whose cellular function is not known. In this study we show that these proteins can substitute for the prokaryotic DNA-bending protein HU in promoting the assembly of the Hin invertasome, an intermediate structure in H...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.7.8.1521
更新日期：1993-08-01 00:00:00
abstract：:RNA polymerase II (Pol II) transcribes all protein-coding genes and many noncoding RNAs in eukaryotic genomes. Although Pol II is a complex, 12-subunit enzyme, it lacks the ability to initiate transcription and cannot consistently transcribe through long DNA sequences. To execute these essential functions, an array of...
journal_title：Genes & development
pub_type： 杂志文章,评审
doi：10.1101/gad.335679.119
更新日期：2020-04-01 00:00:00
