Abstract:
:Transcriptional activation of the human U1 snRNA genes is dependent on a noncanonical octamer element contained within an upstream enhancer. The U1 octamer only weakly recruits the Oct-1 POU domain, although recruitment is stimulated by a peptide containing the Oct-1-binding domain of SNAP190. Structural analysis of the Oct-1 POU domain/U1 octamer/SNAP190 peptide complex revealed that SNAP190 makes extensive protein contacts with the Oct-1 POU-specific domain and with the DNA phosphate backbone within the enhancer. Although SNAP190 and OCA-B both interact with the Oct-1 POU domain through the same Oct-1 interface, a single nucleotide within the U1 octamer ablates OCA-B recruitment without compromising activator recruitment by SNAP190.
journal_name
Genes Devjournal_title
Genes & developmentauthors
Hovde S,Hinkley CS,Strong K,Brooks A,Gu L,Henry RW,Geiger Jdoi
10.1101/gad.1021002subject
Has Abstractpub_date
2002-11-01 00:00:00pages
2772-7issue
21eissn
0890-9369issn
1549-5477journal_volume
16pub_type
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