Isolation of a cDNA encoding the largest subunit of TFIIA reveals functions important for activated transcription.

abstract：:Pre-mRNA processing is coupled with transcription. It is still unclear if the transcription machinery can also directly affect the cytoplasmic fate of a transcript, such as its intracellular localization. In yeast, the RNA-binding protein She2p binds several mRNAs and targets them for localization at the bud. Here we ...

journal_title：Genes & development

authors： Shen Z,St-Denis A,Chartrand P

更新日期：2010-09-01 00:00:00

abstract：:In many species, a dosage compensation complex (DCC) is targeted to X chromosomes of one sex to equalize levels of X-gene products between males (1X) and females (2X). Here we identify cis-acting regulatory elements that target the Caenorhabditis elegans X chromosome for repression by the DCC. The DCC binds to discret...

journal_title：Genes & development

authors： Jans J,Gladden JM,Ralston EJ,Pickle CS,Michel AH,Pferdehirt RR,Eisen MB,Meyer BJ

更新日期：2009-03-01 00:00:00

abstract：:In Saccharomyces cerevisiae, gene silencing at the HMR and HML loci is normally dependent on Sir2p, Sir3p, and Sir4p, which are structural components of silenced chromatin. Sir2p is a NAD+-dependent histone deacetylase required for silencing. Silencing can be restored in cells lacking Sir proteins by a dominant mutati...

journal_title：Genes & development

authors： Rusché LN,Rine J

更新日期：2001-04-15 00:00:00

abstract：:Many small nucleolar RNAs (snoRNAs) in vertebrates are encoded within introns of protein genes. We have reported previously that two isoforms of human U17 snoRNA are encoded in introns of the cell-cycle regulatory gene, RCC1. We have now investigated the mechanism of processing of U17 RNAs and of another intron-encode...

journal_title：Genes & development

authors： Kiss T,Filipowicz W

更新日期：1995-06-01 00:00:00

abstract：:Genetic analysis in Arabidopsis has led to the identification of several genes that are required for auxin response. One of these genes, AXR1, encodes a protein related to yeast Aos1p, a protein that functions to activate the ubiquitin-related protein Smt3p. Here we report the identification of a new gene called TRANS...

journal_title：Genes & development

authors： Ruegger M,Dewey E,Gray WM,Hobbie L,Turner J,Estelle M

更新日期：1998-01-15 00:00:00

abstract：:Transcriptional activation of the prolactin and growth hormone genes, occurring in a cell-specific fashion, requires short-range synergistic interactions between the pituitary-specific POU domain factor Pit-1 and other transcription factors, particularly nuclear receptors. Unexpectedly, we find that these events invol...

journal_title：Genes & development

authors： Holloway JM,Szeto DP,Scully KM,Glass CK,Rosenfeld MG

更新日期：1995-08-15 00:00:00

abstract：:The efficiency of RNA 3'-end formation of spleen necrosis virus (SNV) is determined by the distance between the cap site and the poly(A) site. When the distance between the cap site and the poly(A) site was shorter than 500 bases, only 3-9% of the RNA was polyadenylated at the SNV poly(A) site. However, when the dista...

journal_title：Genes & development

authors： Iwasaki K,Temin HM

更新日期：1990-12-01 00:00:00

abstract：:The mitotic kinase Aurora A (Aur-A) is required for formation of a bipolar mitotic spindle and accurate chromosome segregation. In somatic cells, Aur-A protein and kinase activity levels peak during mitosis, and Aur-A is degraded during mitotic exit. Here, we investigated how Aur-A protein and kinase activity levels a...

journal_title：Genes & development

authors： Littlepage LE,Ruderman JV

更新日期：2002-09-01 00:00:00

abstract：:Identifying modifiers of dosage-sensitive genes involved in neurodegenerative disorders is imperative to discover novel genetic risk factors and potential therapeutic entry points. In this study, we focus on Ataxin-1 (ATXN1), a dosage-sensitive gene involved in the neurodegenerative disease spinocerebellar ataxia type...

journal_title：Genes & development

authors： Nitschke L,Tewari A,Coffin SL,Xhako E,Pang K,Gennarino VA,Johnson JL,Blanco FA,Liu Z,Zoghbi HY

更新日期：2020-09-01 00:00:00

abstract：:Extensive DNA elimination via a DNA breakage and rejoining process occurs during macronuclear development in the hypotrich ciliate Euplotes crassus. The excision process involves the removal of short, unique segments of DNA (internal eliminated sequences; IESs) and at least two highly repetitive families of transposon...

journal_title：Genes & development

authors： Klobutcher LA,Turner LR,LaPlante J

更新日期：1993-01-01 00:00:00

abstract：:We report a systematic mutational analysis of the helix-turn-helix motif (HTH) of the fushi tarazu (ftz) homeo domain (HD) of Drosophila. We started out by testing the function of chimeric ftz proteins containing either a part of the Sex combs reduced (Scr) or the muscle segment homeobox (msh) HDs. By complementation ...

journal_title：Genes & development

authors： Furukubo-Tokunaga K,Müller M,Affolter M,Pick L,Kloter U,Gehring WJ

更新日期：1992-06-01 00:00:00

abstract：:B-cell-specific transcription of immunoglobulin genes is mediated by the interaction of a POU domain containing transcription factor Oct-1 or Oct-2, with the B-cell-specific coactivator OCA-B (Bob-1, OBF-1) and a prototype octamer element. We find that OCA-B binds DNA directly in the major groove between the two subdo...

journal_title：Genes & development

authors： Cepek KL,Chasman DI,Sharp PA

更新日期：1996-08-15 00:00:00

abstract：:Oligophrenin-1 (OPHN1) encodes a Rho-GTPase-activating protein (Rho-GAP) whose loss of function has been associated with X-linked mental retardation (MR). The pathophysiological role of OPHN1, however, remains poorly understood. Here we show that OPHN1 through its Rho-GAP activity plays a critical role in the activity...

journal_title：Genes & development

authors： Nadif Kasri N,Nakano-Kobayashi A,Malinow R,Li B,Van Aelst L

更新日期：2009-06-01 00:00:00

abstract：:Histone post-translational modifications regulate chromatin structure and function largely through interactions with effector proteins that often contain multiple histone-binding domains. While significant progress has been made characterizing individual effector domains, the role of paired domains and how they functi...

journal_title：Genes & development

authors： Rothbart SB,Dickson BM,Ong MS,Krajewski K,Houliston S,Kireev DB,Arrowsmith CH,Strahl BD

更新日期：2013-06-01 00:00:00

abstract：:Genomic imprinting is an epigenetic phenomenon leading to parentally biased gene expression. Throughout the years, extensive efforts have been made to characterize the epigenetic marks underlying imprinting in animals and plants. As a result, DNA methylation asymmetries between parental genomes emerged as the primary ...

journal_title：Genes & development

authors： Batista RA,Köhler C

更新日期：2020-01-01 00:00:00

abstract：:One of the first steps in neurogenesis is the diversification of cells along the dorsoventral axis. In Drosophila the central nervous system develops from three longitudinal columns of cells: ventral cells that express the vnd/nk2 homeobox gene, intermediate cells, and dorsal cells that express the msh homeobox gene. ...

journal_title：Genes & development

authors： Weiss JB,Von Ohlen T,Mellerick DM,Dressler G,Doe CQ,Scott MP

更新日期：1998-11-15 00:00:00

abstract：:MicroRNAs (miRNAs) and transcription factors (TFs) are primary metazoan gene regulators. Whereas much attention has focused on finding the targets of both miRNAs and TFs, the transcriptional networks that regulate miRNA expression remain largely unexplored. Here, we present the first genome-scale Caenorhabditis elegan...

journal_title：Genes & development

authors： Martinez NJ,Ow MC,Barrasa MI,Hammell M,Sequerra R,Doucette-Stamm L,Roth FP,Ambros VR,Walhout AJ

更新日期：2008-09-15 00:00:00

abstract：:Genome-wide association studies (GWASs) have identified a genetic variant of moderate effect size at 6p21.1 associated with erythrocyte traits in humans. We show that this variant affects an erythroid-specific enhancer of CCND3. A Ccnd3 knockout mouse phenocopies these erythroid phenotypes, with a dramatic increase in...

journal_title：Genes & development

authors： Sankaran VG,Ludwig LS,Sicinska E,Xu J,Bauer DE,Eng JC,Patterson HC,Metcalf RA,Natkunam Y,Orkin SH,Sicinski P,Lander ES,Lodish HF

更新日期：2012-09-15 00:00:00

abstract：:The third-chromosome mutation Killer of prune (K-pn) causes no phenotype by itself, but causes lethality in individuals homozygous for the nonlethal X-chromosome mutation prune (pn). We have recovered 12 gamma-ray-induced revertants of Killer of prune. All of the revertants fail to complement a recessive cell lethal m...

journal_title：Genes & development

authors： Biggs J,Tripoulas N,Hersperger E,Dearolf C,Shearn A

更新日期：1988-10-01 00:00:00

abstract：:In enteric bacteria, the transcription factor σ(E) maintains membrane homeostasis by inducing synthesis of proteins involved in membrane repair and two small regulatory RNAs (sRNAs) that down-regulate synthesis of abundant membrane porins. Here, we describe the discovery of a third σ(E)-dependent sRNA, MicL (mRNA-inte...

journal_title：Genes & development

authors： Guo MS,Updegrove TB,Gogol EB,Shabalina SA,Gross CA,Storz G

更新日期：2014-07-15 00:00:00

abstract：:U6 small nuclear RNA is one of the spliceosomal RNAs involved in pre-mRNA splicing. In the fission yeast Schizosaccharomyces pombe, the U6 RNA gene was found to have an intron similar to a nuclear pre-mRNA intron, and it was proposed that the U6 intron might be inserted erroneously during pre-mRNA splicing. Using the ...

journal_title：Genes & development

authors： Tani T,Ohshima Y

更新日期：1991-06-01 00:00:00

abstract：:Cellular senescence limits proliferation of potentially detrimental cells, preventing tumorigenesis and restricting tissue damage. However, the function of senescence in nonpathological conditions is unknown. We found that the human placental syncytiotrophoblast exhibited the phenotype and expressed molecular markers ...

journal_title：Genes & development

authors： Chuprin A,Gal H,Biron-Shental T,Biran A,Amiel A,Rozenblatt S,Krizhanovsky V

更新日期：2013-11-01 00:00:00

abstract：:Cosuppression is a silencing phenomenon triggered by the introduction of homologous DNA sequences into the genomes of organisms as diverse as plants, fungi, flies, and nematodes. Here we report sex-induced silencing (SIS), which is triggered by tandem integration of a transgene array in the human fungal pathogen Crypt...

journal_title：Genes & development

authors： Wang X,Hsueh YP,Li W,Floyd A,Skalsky R,Heitman J

更新日期：2010-11-15 00:00:00

abstract：:The number of known long noncoding RNA (lncRNA) functions is rapidly growing, but how those functions are encoded in their sequence and structure remains poorly understood. NORAD (noncoding RNA activated by DNA damage) is a recently characterized, abundant, and highly conserved lncRNA that is required for proper mitot...

journal_title：Genes & development

authors： Tichon A,Perry RB,Stojic L,Ulitsky I

更新日期：2018-01-01 00:00:00

abstract：:Ssn6-Tup1 regulates multiple genes in yeast, providing a paradigm for corepressor functions. Tup1 interacts directly with histones H3 and H4, and mutation of these histones synergistically compromises Ssn6-Tup1-mediated repression. In vitro, Tup1 interacts preferentially with underacetylated isoforms of H3 and H4, sug...

journal_title：Genes & development

authors： Watson AD,Edmondson DG,Bone JR,Mukai Y,Yu Y,Du W,Stillman DJ,Roth SY

更新日期：2000-11-01 00:00:00

abstract：:Caenorhabditis elegans let-7, a founding member of the microRNA family, is predicted to bind to six sites in the 3'UTR of the mRNA of its target gene, lin-41, to down-regulate LIN-41. Here, we demonstrate that wild-type let-7 microRNA binds in vitro to RNA from the lin-41 3'UTR. This interaction is dependent on two co...

journal_title：Genes & development

authors： Vella MC,Choi EY,Lin SY,Reinert K,Slack FJ

更新日期：2004-01-15 00:00:00

abstract：:The Caenorhabditis elegans caudal homolog, pal-1, is required for neurogenesis in the male tail. We show that expression of pal-1 in the postembryonic neuroblast cell V6 can be initiated by two alternate pathways. One pathway, acting in wild type, requires a regulatory element in the fifth pal-1 intron. The other path...

journal_title：Genes & development

authors： Zhang H,Emmons SW

更新日期：2000-09-01 00:00:00

abstract：:Protein kinases are key regulators of cellular processes. In spite of considerable effort, a full understanding of the pathways they participate in remains elusive. We globally investigated the proteins that interact with the majority of yeast protein kinases using protein microarrays. Eighty-five kinases were purifie...

journal_title：Genes & development

authors： Fasolo J,Sboner A,Sun MG,Yu H,Chen R,Sharon D,Kim PM,Gerstein M,Snyder M

更新日期：2011-04-01 00:00:00

abstract：:O(6)-Methylguanine ((Me)G) is a highly cytotoxic DNA modification generated by S(N)1-type methylating agents. Despite numerous studies implicating DNA replication, mismatch repair (MMR), and homologous recombination (HR) in (Me)G toxicity, its mode of action has remained elusive. We studied the molecular transactions ...

journal_title：Genes & development

authors： Mojas N,Lopes M,Jiricny J

更新日期：2007-12-15 00:00:00

abstract：:The gene encoding yeast U6 snRNA that is transcribed by RNA polymerase III (Pol III) contains both a TATA box upstream of the transcription start site and a downstream binding site for the factor TFIIIC. This juxtaposition of elements typical of both Pol II- and Pol III-transcribed genes raises the question of how pol...

journal_title：Genes & development

authors： Roberts S,Colbert T,Hahn S

更新日期：1995-04-01 00:00:00