Abstract：
:MicroRNAs (miRNAs) and transcription factors (TFs) are primary metazoan gene regulators. Whereas much attention has focused on finding the targets of both miRNAs and TFs, the transcriptional networks that regulate miRNA expression remain largely unexplored. Here, we present the first genome-scale Caenorhabditis elegans miRNA regulatory network that contains experimentally mapped transcriptional TF --> miRNA interactions, as well as computationally predicted post-transcriptional miRNA --> TF interactions. We find that this integrated miRNA network contains 23 miRNA <--> TF composite feedback loops in which a TF that controls a miRNA is itself regulated by that same miRNA. By rigorous network randomizations, we show that such loops occur more frequently than expected by chance and, hence, constitute a genuine network motif. Interestingly, miRNAs and TFs in such loops are heavily regulated and regulate many targets. This "high flux capacity" suggests that loops provide a mechanism of high information flow for the coordinate and adaptable control of miRNA and TF target regulons.
journal_name
Genes Devjournal_title
Genes & developmentauthors
Martinez NJ,Ow MC,Barrasa MI,Hammell M,Sequerra R,Doucette-Stamm L,Roth FP,Ambros VR,Walhout AJdoi
10.1101/gad.1678608subject
Has Abstractpub_date
2008-09-15 00:00:00pages
2535-49issue
18eissn
0890-9369issn
1549-5477pii
22/18/2535journal_volume
22pub_type
杂志文章abstract：:Discrete sequence elements known as exonic splicing enhancers (ESEs) have been shown to influence both the efficiency of splicing and the profile of mature mRNAs in multicellular eukaryotes. While the existence of ESEs has not been demonstrated previously in unicellular eukaryotes, the factors known to recognize these...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.1265905
更新日期：2005-01-15 00:00:00
abstract：:The p53 tumor suppressor is often disrupted in human cancers by the acquisition of missense mutations. We generated mice with a missense mutation at codon 172 that mimics the p53R175H hot spot mutation in human cancer. p53 homozygous mutant mice have unstable mutant p53 in normal cells and stabilize mutant p53 in some...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.1662908
更新日期：2008-05-15 00:00:00
abstract：:Most eukaryotic mRNAs are translated using a cap-dependent mechanism of translation. However, approximately 10% of mammalian mRNAs initiate translation using a cap-independent mechanism that is not well understood. These mRNAs contain an internal ribosome entry site (IRES) located in the 5' untranslated region. The cr...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.1832209
更新日期：2009-12-01 00:00:00
abstract：:The Bloom's syndrome gene (BLM) plays a pivotal role in the maintenance of genomic stability in somatic cells. It encodes a DNA helicase (BLM) of the RecQ family, but the exact function of BLM remains elusive. To study this question, we have cloned the BLM homolog of the frog Xenopus laevis (xBLM) and have raised anti...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.822400
更新日期：2000-10-15 00:00:00
abstract：:Telomeres protect the ends of linear chromosomes from degradation and abnormal recombination events, and in vertebrates may be important in cellular senescence and cancer. However, very little is known about the structure of human telomeres. In this report we purify telomeres and analyze their termini. We show that fo...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.11.21.2801
更新日期：1997-11-01 00:00:00
abstract：:Post-translational processes are essential for the generation and dynamics of mammalian circadian rhythms. In particular, phosphorylation of the key circadian protein PER2 precisely controls the period and phase of circadian oscillations. However, the mechanisms underlying that control are poorly understood. Here, we ...
journal_title：Genes & development
pub_type： 杂志文章
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journal_title：Genes & development
pub_type： 杂志文章
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更新日期：2014-08-01 00:00:00
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journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.321004
更新日期：2004-11-01 00:00:00
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journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.212738.112
更新日期：2013-03-15 00:00:00
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journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.13.1.64
更新日期：1999-01-01 00:00:00
abstract：:Transcription of bacteriophage N4 middle genes is carried out by a phage-coded, heterodimeric RNA polymerase (N4 RNAPII), which belongs to the family of T7-like RNA polymerases. In contrast to phage T7-RNAP, N4 RNAPII displays no activity on double-stranded templates and low activity on single-stranded templates. In v...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.1121403
更新日期：2003-09-15 00:00:00
abstract：:The ascidian tadpole represents the most simplified chordate body plan. It contains a notochord composed of just 40 cells, but as in vertebrates Brachyury is essential for notochord differentiation. Here, we show that the misexpression of the Brachyury gene (Ci-Bra) of Ciona intestinalis is sufficient to transform end...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.13.12.1519
更新日期：1999-06-15 00:00:00
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journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.1286005
更新日期：2005-04-01 00:00:00
abstract：:The rpoH gene of Escherichia coli encodes sigma 32, the 32-kD sigma-factor responsible for the heat-inducible transcription of the heat shock genes. rpoH is transcribed from at least three promoters. Two of these promoters are recognized by RNA polymerase containing sigma 70, the predominant sigma-factor. We purified ...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.3.9.1462
更新日期：1989-09-01 00:00:00
abstract：:The tobacco mitogen-activated protein kinase (MAPK) cascade, which includes MAPK NRK1/NTF6, positively regulates expansion of the cytokinetic machinery known as the phragmoplast, which is followed by the synthesis of cell plates for completion of cell division. However, molecular events lying between the MAPK and phra...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.1408106
更新日期：2006-04-15 00:00:00
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journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.997902
更新日期：2002-08-01 00:00:00
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journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.12.13.1953
更新日期：1998-07-01 00:00:00
abstract：:SpoIIIE is an FtsK-related protein that transports the forespore chromosome across the Bacillus subtilis sporulation septum. We use membrane photobleaching and protoplast assays to demonstrate that SpoIIIE is required for septal membrane fission in the presence of trapped DNA, and that DNA is transported across separa...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.1925210
更新日期：2010-06-01 00:00:00
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journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.11.5.663
更新日期：1997-03-01 00:00:00
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journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.917101
更新日期：2001-11-01 00:00:00
abstract：:Treatment with retinoic acid (RA) is known to produce complex teratogenic effects in vertebrates, and its presence in the developing embryo as an endogenous substance has led to the suggestion that RA might be a natural morphogenetic agent. Although our understanding of the molecular mechanism of RA action has improve...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.5.8.1333
更新日期：1991-08-01 00:00:00
abstract：:The activity of the polyoma virus (PyV) origin of DNA replication was used as a sensitive assay for enhancer function in one- and two-cell mouse embryos by injecting embryos with plasmid DNA containing different PyV ori configurations, allowing them to continue development in vitro, and then measuring plasmid DNA repl...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.2.9.1115
更新日期：1988-09-01 00:00:00
abstract：:Start sites of DNA replication are marked by the origin recognition complex (ORC), which coordinates Mcm2-7 helicase loading to form the prereplicative complex (pre-RC). Although pre-RC assembly is well characterized in vitro, the process is poorly understood within the local chromatin environment surrounding replicat...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.247924.114
更新日期：2015-01-15 00:00:00
abstract：:Transabdominal (Tab), a dominant mutation in the Bithorax Complex (BX-C) of Drosophila, creates a sexually dimorphic pattern of segmental transformation that has complete penetrance and expressivity. Specific regions within the notum of the second thoracic segment (T2) are transformed into abdominal-like cuticle; thus...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.1.2.111
更新日期：1987-04-01 00:00:00
abstract：:The yeast SAGA (Spt-Ada-Gcn5-acetyltransferase) coactivator complex exerts functions in gene expression, including activator interaction, histone acetylation, histone deubiquitination, mRNA export, chromatin recognition, and regulation of the basal transcription machinery. These diverse functions involve distinct modu...
journal_title：Genes & development
pub_type： 杂志文章,评审
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更新日期：2012-03-15 00:00:00
abstract：:The physiological functions of many vital tissues and organs continue to mature after birth, but the genetic mechanisms governing this postnatal maturation remain an unsolved mystery. Human pancreatic β cells produce and secrete insulin in response to physiological cues like glucose, and these hallmark functions impro...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.342378.120
更新日期：2021-01-14 00:00:00
abstract：:By studying neuroectoderm formation in the absence of mesoderm and mesectoderm in mutants of the zygotic genes snail and twist, we have found that the number of neuroblasts is not reduced in these mutants, suggesting that mesoderm and mesectoderm are not essential for the initiation of neural development. The position...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.5.9.1577
更新日期：1991-09-01 00:00:00
abstract：:Our understanding of eukaryotic transcriptional activation mechanisms has been hampered by an inability to identify the direct in vivo targets of activator proteins, primarily because of lack of appropriate experimental methods. To circumvent this problem, we have developed a fluorescence resonance energy transfer (FR...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.1148404
更新日期：2004-02-01 00:00:00
abstract：:The MMTVD/myc transgenic mice spontaneously develop oligoclonal CD4+CD8+ T-cell tumors. We used provirus insertional mutagenesis in these mice to identify putative collaborators of c-myc. We found that Notch1 was mutated in a high proportion (52%) of these tumors. Proviruses were inserted upstream of the exon coding f...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.10.15.1930
更新日期：1996-08-01 00:00:00
abstract：:The mottler of white (mw) locus has been determined to interact with alleles of the white (w) eye color locus which are a subset of the transposable element insertion mutants. The transposable elements belong to six different types, including copia, and are located at several sites within the w gene. Three X-ray-induc...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.3.1.73
更新日期：1989-01-01 00:00:00
