In vivo target of a transcriptional activator revealed by fluorescence resonance energy transfer.

abstract：:The mottler of white (mw) locus has been determined to interact with alleles of the white (w) eye color locus which are a subset of the transposable element insertion mutants. The transposable elements belong to six different types, including copia, and are located at several sites within the w gene. Three X-ray-induc...

journal_title：Genes & development

authors： Birchler JA,Hiebert JC,Rabinow L

更新日期：1989-01-01 00:00:00

abstract：:Class Ia phosphatidylinositol 3 kinase (PI3K) is required for oncogenic receptor-mediated transformation; however, the individual roles of the two commonly expressed class Ia PI3K isoforms in oncogenic receptor signaling have not been elucidated in vivo. Here, we show that genetic ablation of p110α blocks tumor format...

journal_title：Genes & development

authors： Utermark T,Rao T,Cheng H,Wang Q,Lee SH,Wang ZC,Iglehart JD,Roberts TM,Muller WJ,Zhao JJ

更新日期：2012-07-15 00:00:00

abstract：:Chromatin is organized into higher-order structures that form subcompartments in interphase nuclei. Different categories of specialized enzymes act on chromatin and regulate its compaction and biophysical characteristics in response to physiological conditions. We present an overview of the function of chromatin struc...

journal_title：Genes & development

authors： Hauer MH,Gasser SM

更新日期：2017-11-15 00:00:00

abstract：:The Arabidopsis APETALA3 (AP3) and PISTILLATA (PI) proteins are thought to act as transcription factors and are required for specifying floral organ identities. To define the nuclear localization signals within these proteins, we generated translational fusions of the coding regions of AP3 and PI to the bacterial uidA...

journal_title：Genes & development

authors： McGonigle B,Bouhidel K,Irish VF

更新日期：1996-07-15 00:00:00

abstract：:The lysosomal storage disorder galactosialidosis results from a primary deficiency of the protective protein/cathepsin A (PPCA), which in turn affects the activities of beta-galactosidase and neuraminidase. Mice homozygous for a null mutation at the PPCA locus present with signs of the disease shortly after birth and ...

journal_title：Genes & development

authors： Zhou XY,Morreau H,Rottier R,Davis D,Bonten E,Gillemans N,Wenger D,Grosveld FG,Doherty P,Suzuki K,Grosveld GC,d'Azzo A

更新日期：1995-11-01 00:00:00

abstract：:The Rad51 and Lim15 proteins of lily, which are homologs of the bacterial RecA protein, were found on chromosomes in various stages of meiotic prophase 1. The presence of both Rad51 and Lim15 proteins as discrete foci on leptotene and zygotene chromosomes and their colocalization suggest that meiotic recombination beg...

journal_title：Genes & development

authors： Terasawa M,Shinohara A,Hotta Y,Ogawa H,Ogawa T

更新日期：1995-04-15 00:00:00

abstract：:The mammalian ATF/CREB family of transcription factors comprises a large group of basic-region leucine zipper (bZIP) proteins whose members mediate diverse transcriptional regulatory functions. Here we report that expression of a specific mouse ATF gene, ATFx, is down-regulated in a variety of cells undergoing apoptos...

journal_title：Genes & development

authors： Persengiev SP,Devireddy LR,Green MR

更新日期：2002-07-15 00:00:00

abstract：:During vasculogenesis and angiogenesis, endothelial cell responses to growth factors are modulated by the compositional and mechanical properties of a surrounding three-dimensional (3D) extracellular matrix (ECM) that is dominated by either cross-linked fibrin or type I collagen. While 3D-embedded endothelial cells es...

journal_title：Genes & development

authors： Zhou X,Rowe RG,Hiraoka N,George JP,Wirtz D,Mosher DF,Virtanen I,Chernousov MA,Weiss SJ

更新日期：2008-05-01 00:00:00

abstract：:Here we show that the cell cycle defects of dE2F1-depleted cells depend on the cooperative effects of dE2F2 and DACAPO (DAP), an inhibitor of Cyclin E/cyclin-dependent kinase 2 (CycE/cdk2). The different properties of cells lacking dE2F1/dE2F2 and dE2F1/DAP lead to the surprising observation that dE2F2-mediated repres...

journal_title：Genes & development

authors： Frolov MV,Stevaux O,Moon NS,Dimova D,Kwon EJ,Morris EJ,Dyson NJ

更新日期：2003-03-15 00:00:00

abstract：:Alternative splicing factor/splicing factor 2 (ASF/SF2) is the prototype of a family of nuclear proteins highly conserved throughout metazoa, the SR (serine/arginine) proteins. Based largely on in vitro studies, SR proteins have been suggested to play important roles in constitutive and alternative splicing of pre-mRN...

journal_title：Genes & development

authors： Wang J,Takagaki Y,Manley JL

更新日期：1996-10-15 00:00:00

abstract：:RNA polymerase II (Pol II) transcribes all protein-coding genes and many noncoding RNAs in eukaryotic genomes. Although Pol II is a complex, 12-subunit enzyme, it lacks the ability to initiate transcription and cannot consistently transcribe through long DNA sequences. To execute these essential functions, an array of...

journal_title：Genes & development

authors： Schier AC,Taatjes DJ

更新日期：2020-04-01 00:00:00

abstract：:The distal enhancer of the T-cell receptor (TCR) alpha chain gene has become a paradigm for studies of the assembly and activity of architectural enhancer complexes. Here we have reconstituted regulated TCR alpha enhancer activity in vitro on chromatin templates using purified T-cell transcription factors (LEF-1, AML1...

journal_title：Genes & development

authors： Mayall TP,Sheridan PL,Montminy MR,Jones KA

更新日期：1997-04-01 00:00:00

abstract：:The heterochromatin-like structure formed by the yeast silent information regulator complex (SIR) represses transcription at the silent mating type loci and telomeres. Here, we report that tight protein-DNA complexes induce ectopic recruitment of the SIR complex, promoting gene silencing and changes in subnuclear loca...

journal_title：Genes & development

authors： Dubarry M,Loïodice I,Chen CL,Thermes C,Taddei A

更新日期：2011-07-01 00:00:00

abstract：:Transcriptional activation of the human U1 snRNA genes is dependent on a noncanonical octamer element contained within an upstream enhancer. The U1 octamer only weakly recruits the Oct-1 POU domain, although recruitment is stimulated by a peptide containing the Oct-1-binding domain of SNAP190. Structural analysis of t...

journal_title：Genes & development

authors： Hovde S,Hinkley CS,Strong K,Brooks A,Gu L,Henry RW,Geiger J

更新日期：2002-11-01 00:00:00

abstract：:Historically, biomedical research has been based on two paradigms. First, measurements of biological behaviors have been based on bulk assays that average over large populations. Second, these behaviors have then been crudely perturbed by systemic administration of therapeutic treatments. Nanotechnology has the potent...

journal_title：Genes & development

authors： Wong IY,Bhatia SN,Toner M

更新日期：2013-11-15 00:00:00

abstract：:We have identified protein kinase C-zeta (PKC-zeta) as a novel suppressor of neoplastic transformation caused by the v-raf oncogene. PKC-zeta overexpression drastically retards proliferation, abolishes anchorage-independent growth, and reverts the morphological transformation of v-raf-transformed NIH-3T3 cells. The mo...

journal_title：Genes & development

authors： Kieser A,Seitz T,Adler HS,Coffer P,Kremmer E,Crespo P,Gutkind JS,Henderson DW,Mushinski JF,Kolch W,Mischak H

更新日期：1996-06-15 00:00:00

abstract：:Integrin-mediated cell-matrix interactions are essential for development, tissue homeostasis, and repair. Upon ligand binding, integrins are recruited into focal adhesions (FAs). Integrin-linked kinase (ILK) is an FA component that interacts with the cytoplasmic domains of integrins, recruits adaptor proteins that lin...

journal_title：Genes & development

authors： Sakai T,Li S,Docheva D,Grashoff C,Sakai K,Kostka G,Braun A,Pfeifer A,Yurchenco PD,Fässler R

更新日期：2003-04-01 00:00:00

abstract：:We describe here a new murine homeo box gene, denoted Hlx, which is expressed within specific hematopoietic lineages. The cDNA sequence indicates that Hlx differs markedly from known vertebrate homeo box genes, and linkage analysis of an interspecific murine backcross showed that it resides at a novel homeo box locus ...

journal_title：Genes & development

authors： Allen JD,Lints T,Jenkins NA,Copeland NG,Strasser A,Harvey RP,Adams JM

更新日期：1991-04-01 00:00:00

abstract：:The hair follicle undergoes a lifelong developmental cycle that depends on the integration between activating and inhibitory signals acting to regulate and guide the proliferation and differentiation of pluripotent epithelial stem cells. The effectors and mechanisms responsible for re-entry of quiescent telogen hair f...

journal_title：Genes & development

authors： Coulombe PA,Caterina MJ

更新日期：2013-06-15 00:00:00

abstract：:Histone post-translational modifications regulate chromatin structure and function largely through interactions with effector proteins that often contain multiple histone-binding domains. While significant progress has been made characterizing individual effector domains, the role of paired domains and how they functi...

journal_title：Genes & development

authors： Rothbart SB,Dickson BM,Ong MS,Krajewski K,Houliston S,Kireev DB,Arrowsmith CH,Strahl BD

更新日期：2013-06-01 00:00:00

abstract：:Mammalian cells exhibit a complex response to DNA damage. The tumor suppressor BRCA1 and associated protein BARD1 are thought to play an important role in this response, and our previous work demonstrated that this includes transient inhibition of the pre-mRNA 3' processing machinery. Here we provide evidence that thi...

journal_title：Genes & development

authors： Kleiman FE,Wu-Baer F,Fonseca D,Kaneko S,Baer R,Manley JL

更新日期：2005-05-15 00:00:00

abstract：:PBAF and BAF are two chromatin-remodeling complexes of the SWI/SNF family essential for mammalian transcription and development. Although these complexes share eight identical subunits, only PBAF can facilitate transcriptional activation by nuclear receptors in vitro. Here we show that these complexes have selectivity...

journal_title：Genes & development

authors： Yan Z,Cui K,Murray DM,Ling C,Xue Y,Gerstein A,Parsons R,Zhao K,Wang W

更新日期：2005-07-15 00:00:00

abstract：:The LIM motif defines a double zinc finger structure found in proteins involved in cell fate determination and growth control. LIM proteins, which include LIM homeo domain, LIM kinase, focal adhesion, and LIM-only proteins, usually contain two or more LIM motifs clustered at their amino- or carboxy-terminal end. At pr...

journal_title：Genes & development

authors： Arber S,Caroni P

更新日期：1996-02-01 00:00:00

abstract：:The integration of viral DNA into the host genome is an essential step in the retrovirus life cycle. To understand this process better, we have examined the native state of viral DNA in cells acutely infected by murine leukemia virus (MLV), using both a physical assay for viral DNA and a functional assay for integrati...

journal_title：Genes & development

authors： Bowerman B,Brown PO,Bishop JM,Varmus HE

更新日期：1989-04-01 00:00:00

abstract：:Cascades of zygotic gene expression pattern the anterior-posterior (AP) and dorsal-ventral (DV) axes of the early Drosophila embryo. Here, we used the global run-on sequencing assay (GRO-seq) to map the genome-wide RNA polymerase distribution during early Drosophila embryogenesis, thus providing insights into how gene...

journal_title：Genes & development

authors： Saunders A,Core LJ,Sutcliffe C,Lis JT,Ashe HL

更新日期：2013-05-15 00:00:00

abstract：:How muscle diversity is generated in the vertebrate body is poorly understood. In the limb, dorsal and ventral muscle masses constitute the first myogenic diversification, as each gives rise to distinct muscles. Myogenesis initiates after muscle precursor cells (MPCs) have migrated from the somites to the limb bud and...

journal_title：Genes & development

authors： Anderson C,Williams VC,Moyon B,Daubas P,Tajbakhsh S,Buckingham ME,Shiroishi T,Hughes SM,Borycki AG

更新日期：2012-09-15 00:00:00

abstract：:The homeless (hls) gene of Drosophila is required for anteroposterior and dorsoventral axis formation during oogenesis. At a low frequency, females homozygous for mutations in hls generate early egg chambers in which the oocyte is positioned incorrectly within the cyst. At a high frequency, late-stage egg chambers exh...

journal_title：Genes & development

authors： Gillespie DE,Berg CA

更新日期：1995-10-15 00:00:00

abstract：:In many eukaryotes, siRNAs bound to Argonaute proteins guide chromatin-modifying enzymes to complementary loci, resulting in transcriptional gene silencing. Multiple lines of evidence indicate that siRNAs base-pair with longer RNAs produced at target loci, but the possibility that siRNAs base-pair directly with DNA re...

journal_title：Genes & development

authors： Wendte JM,Pikaard CS

更新日期：2016-12-15 00:00:00

abstract：:Fgf-10-deficient mice (Fgf-10(-/-)) were generated to determine the role(s) of Fgf-10 in vertebrate development. Limb bud initiation was abolished in Fgf-10(-/-) mice. Strikingly, Fgf-10(-/-) fetuses continued to develop until birth, despite the complete absence of both fore- and hindlimbs. Fgf-10 is necessary for api...

journal_title：Genes & development

authors： Min H,Danilenko DM,Scully SA,Bolon B,Ring BD,Tarpley JE,DeRose M,Simonet WS

更新日期：1998-10-15 00:00:00

abstract：:Cap-dependent translation is mediated by eIF4F, a protein complex composed of three subunits as follows: eIF4E, which recognizes the mRNA 5' cap structure; eIF4A, an RNA-helicase; and eIF4G, a scaffolding protein that binds eIF4E, eIF4A, and the eIF4E-kinase Mnk1 simultaneously. eIF4E is hypophosphorylated and cap-dep...

journal_title：Genes & development

authors： Pyronnet S,Dostie J,Sonenberg N

更新日期：2001-08-15 00:00:00