Abstract:
:Here we show that the cell cycle defects of dE2F1-depleted cells depend on the cooperative effects of dE2F2 and DACAPO (DAP), an inhibitor of Cyclin E/cyclin-dependent kinase 2 (CycE/cdk2). The different properties of cells lacking dE2F1/dE2F2 and dE2F1/DAP lead to the surprising observation that dE2F2-mediated repression differs from retinoblastoma family protein 1 (RBF1) inhibition of dE2F1, and is resistant to both CycE/cdk2 and Cyclin D/cyclin-dependent kinase 4 (CycD/cdk4). This resistance occurs even though dE2F2/RBF1 complexes are disrupted by CycE/cdk2, and may explain why dE2F2 is so potent in the absence of de2f1. The implication of these results is that cells containing dE2F2 require dE2F1 to either prevent, or reverse, dE2F-mediated repression.
journal_name
Genes Devjournal_title
Genes & developmentauthors
Frolov MV,Stevaux O,Moon NS,Dimova D,Kwon EJ,Morris EJ,Dyson NJdoi
10.1101/gad.1031803subject
Has Abstractpub_date
2003-03-15 00:00:00pages
723-8issue
6eissn
0890-9369issn
1549-5477journal_volume
17pub_type
杂志文章abstract::Early during Dictyostelium development a fundamental cell-fate decision establishes the anteroposterior (prestalk/prespore) axis. Signaling via the 7-transmembrane cAMP receptor CAR4 is essential for creating and maintaining a normal pattern; car4-null alleles have decreased levels of prestalk-specific mRNAs but enhan...
journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.11.16.2112
更新日期:1997-08-15 00:00:00
abstract::Endospores of Bacillus subtilis are encased in a two-layer protein shell known as the coat, which consists of a lammellar-like inner layer and an electron-dense outer layer. We report the cloning of the structural gene (designated cotE) for an alkali-soluble coat protein of 24 kD and show that the cotE gene product is...
journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.2.8.1047
更新日期:1988-08-01 00:00:00
abstract::In yeast, galactose triggers a rapid GAL4-dependent induction of galactose/melibiose regulon (GAL/MEL) gene transcription, and glucose represses this activation. We discovered that alterations in the physical state of the GAL4 protein correlate with activation and repression of the GAL/MEL genes. Using Western immunob...
journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.3.8.1157
更新日期:1989-08-01 00:00:00
abstract::The empty spiracles (ems) homeo box gene is required for the development of the Drosophila larval filzkörper, which are structural specializations of the eighth abdominal segment. Filzkörper development is also dependent on the function of the homeotic selector gene Abdominal-B (Abd-B). Here, we show that ems is a dow...
journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.7.2.229
更新日期:1993-02-01 00:00:00
abstract::A novel histone methyltransferase, termed Set9, was isolated from human cells. Set9 contains a SET domain, but lacks the pre- and post-SET domains. Set9 methylates specifically lysine 4 (K4) of histone H3 (H3-K4) and potentiates transcription activation. The histone H3 tail interacts specifically with the histone deac...
journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.967202
更新日期:2002-02-15 00:00:00
abstract::Children with trisomy 21/Down syndrome (DS) are at high risk to develop acute megakaryoblastic leukemia (DS-AMKL) and the related transient leukemia (DS-TL). The factors on human chromosome 21 (Hsa21) that confer this predisposing effect, especially in synergy with consistently mutated transcription factor GATA1 (GATA...
journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.1856210
更新日期:2010-03-01 00:00:00
abstract::The balance between proliferation and differentiation of muscle stem cells is tightly controlled, ensuring the maintenance of a cellular pool needed for muscle growth and repair. We demonstrate here that the transcriptional regulator Hes1 controls the balance between proliferation and differentiation of activated musc...
journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.322818.118
更新日期:2019-05-01 00:00:00
abstract::Genomic imprinting is essential for mammalian development. Recent studies have revealed that maternal histone H3 Lys27 trimethylation (H3K27me3) can mediate DNA methylation-independent genomic imprinting. However, the regulatory mechanisms and functions of this new imprinting mechanism are largely unknown. Here we dem...
journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.318675.118
更新日期:2018-12-01 00:00:00
abstract::The gene encoding yeast U6 snRNA that is transcribed by RNA polymerase III (Pol III) contains both a TATA box upstream of the transcription start site and a downstream binding site for the factor TFIIIC. This juxtaposition of elements typical of both Pol II- and Pol III-transcribed genes raises the question of how pol...
journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.9.7.832
更新日期:1995-04-01 00:00:00
abstract::Cyclin D1 is part of a cell cycle control node consistently deregulated in most human cancers. However, studies with cyclin D1-null mice indicate that it is dispensable for normal mouse development as well as cell growth in culture. Here, we provide evidence that ras-mediated tumorigenesis depends on signaling pathway...
journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.12.16.2469
更新日期:1998-08-15 00:00:00
abstract::Covalent modifications of the histone N tails play important roles in eukaryotic gene expression. Histone acetylation, in particular, is required for the activation of a subset of eukaryotic genes through the targeted recruitment of histone acetyltransferases. We have reported that a histone C tail modification, ubiqu...
journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.1149604
更新日期:2004-01-15 00:00:00
abstract::The p53 tumor suppressor is often disrupted in human cancers by the acquisition of missense mutations. We generated mice with a missense mutation at codon 172 that mimics the p53R175H hot spot mutation in human cancer. p53 homozygous mutant mice have unstable mutant p53 in normal cells and stabilize mutant p53 in some...
journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.1662908
更新日期:2008-05-15 00:00:00
abstract::Neurofibromatosis type 1 (NF1) is a common neurodevelopmental disorder caused by impaired function of the neurofibromin RAS regulator. Using a combination of Nf1 genetically engineered mice and pharmacological/genetic inhibition approaches, we report that neurofibromin differentially controls neural stem cell (NSC) pr...
journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.261677.115
更新日期:2015-08-15 00:00:00
abstract::Hepatitis B virus (HBV) infects humans and causes a wide range of clinical manifestations, from acute hepatitis to hepatocellular carcinoma (HCC). The HBV genome contains multiple promoters with gene expression regulated predominantly by the cellular transcription initiation machinery. Accordingly, the HBV-encoded pX,...
journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.12.8.1217
更新日期:1998-04-15 00:00:00
abstract::For a number of human genes that encode transcripts containing inverted repeat Alu elements (IRAlus) within their 3' untranslated region (UTR), product mRNA is efficiently exported to the cytoplasm when the IRAlus, which mediate nuclear retention, are removed by alternative polyadenylation. Here we report a new mechan...
journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.220962.113
更新日期:2013-07-01 00:00:00
abstract::B-cell-specific transcription of immunoglobulin genes is mediated by the interaction of a POU domain containing transcription factor Oct-1 or Oct-2, with the B-cell-specific coactivator OCA-B (Bob-1, OBF-1) and a prototype octamer element. We find that OCA-B binds DNA directly in the major groove between the two subdo...
journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.10.16.2079
更新日期:1996-08-15 00:00:00
abstract::The Agrobacterium Ti plasmid (T-DNA) 6b proteins interact with many different host proteins implicated in plant cell proliferation. Here, we show that Arabidopsis plants overexpressing 6b display microRNA (miRNA) deficiency by directly targeting SERRATE and AGO1 via a specific loop fragment (residues 40-55). In additi...
journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.1985511
更新日期:2011-01-01 00:00:00
abstract::Protein kinases are key regulators of cellular processes. In spite of considerable effort, a full understanding of the pathways they participate in remains elusive. We globally investigated the proteins that interact with the majority of yeast protein kinases using protein microarrays. Eighty-five kinases were purifie...
journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.1998811
更新日期:2011-04-01 00:00:00
abstract::Intron homing in phage T4 occurs in the context of recombination-dependent replication, by virtue of intron-encoded endonucleolytic activity. After the td intron endonuclease I-TevI cleaves the intronless recipient 23 and 25 nucleotides upstream of the intron insertion site, exonucleolytic degradation is required for ...
journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.10.17.2158
更新日期:1996-09-01 00:00:00
abstract::The essential splicing factor human UAP56 (hUAP56) is a DExD/H-box protein known to promote prespliceosome assembly. Here, using a series of hUAP56 mutants that are defective for ATP-binding, ATP hydrolysis, or dsRNA unwindase/helicase activity, we assess the relative contributions of these biochemical functions to pr...
journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.1657308
更新日期:2008-07-01 00:00:00
abstract::Experiments in Xenopus have illustrated the importance of extracellular morphogens for embryonic gene regulation in vertebrates. Much less is known about how induction leads to the correct positioning of boundaries; for example, between germ layers. Here we report that the neuroectoderm/mesoderm boundary is controlled...
journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.409507
更新日期:2007-04-15 00:00:00
abstract::Human lymphoblastoid cells derived from different healthy individuals display considerable variation in their transcription profiles. Here we show that such variation in gene expression underlies interindividual susceptibility to DNA damaging agents. The results demonstrate the massive differences in sensitivity acros...
journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.1688508
更新日期:2008-10-01 00:00:00
abstract::In Saccharomyces cerevisiae, the genes encoding the HO endonuclease, G1-specific cyclins CLN1 and CLN2, as well as most proteins involved in DNA synthesis, are periodically transcribed with maximal levels reached in late G1. For HO and the DNA replication genes, cell cycle stage-specific expression has been shown to b...
journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.6.4.557
更新日期:1992-04-01 00:00:00
abstract::The MMTVD/myc transgenic mice spontaneously develop oligoclonal CD4+CD8+ T-cell tumors. We used provirus insertional mutagenesis in these mice to identify putative collaborators of c-myc. We found that Notch1 was mutated in a high proportion (52%) of these tumors. Proviruses were inserted upstream of the exon coding f...
journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.10.15.1930
更新日期:1996-08-01 00:00:00
abstract::The cJun N-terminal kinase 1 (JNK1) is implicated in diet-induced obesity. Indeed, germline ablation of the murine Jnk1 gene prevents diet-induced obesity. Here we demonstrate that selective deficiency of JNK1 in the murine nervous system is sufficient to suppress diet-induced obesity. The failure to increase body mas...
journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.1878510
更新日期:2010-02-01 00:00:00
abstract::We describe the developmental effects of the expression of a normal and mutated Drosophila ras gene ras2 in transgenic Drosophila. A Gly14----Val14 mutant (Gly14 in ras2 is equivalent to Gly12 in mammalian ras proteins) was constructed in ras2 by site-directed mutagenesis. Inducible constructs of ras2 and ras2Val14 we...
journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.2.5.567
更新日期:1988-05-01 00:00:00
abstract::The tobacco mitogen-activated protein kinase (MAPK) cascade, which includes MAPK NRK1/NTF6, positively regulates expansion of the cytokinetic machinery known as the phragmoplast, which is followed by the synthesis of cell plates for completion of cell division. However, molecular events lying between the MAPK and phra...
journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.1408106
更新日期:2006-04-15 00:00:00
abstract::Distinctive from that of the animal system, the basic plan of the plant body is the continuous formation of a structural unit, composed of a stem with a meristem at the top and lateral organs continuously forming at the meristem. Therefore, mechanisms controlling the formation, maintenance, and development of a merist...
journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.13.9.1079
更新日期:1999-05-01 00:00:00
abstract::Genetic screening and selection procedures employing a secA-lacZ fusion strain repeatedly have yielded mutations in four genes affecting the protein export pathway of Escherichia coli. These genes are secA, secD, prlA/secY, and secE. We discuss the significance of the failure to find new sec genes after extensive use ...
journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.3.7.1035
更新日期:1989-07-01 00:00:00
abstract::Outward migration of epidermal progenitors occurs with induction of hundreds of differentiation genes, but the identities of all regulators required for this process are unknown. We used laser capture microdissection followed by RNA sequencing to identify calmodulin-like 5 (CALML5) as the most enriched gene in differe...
journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.267708.115
更新日期:2015-11-01 00:00:00