Abstract：
:Histone post-translational modifications regulate chromatin structure and function largely through interactions with effector proteins that often contain multiple histone-binding domains. While significant progress has been made characterizing individual effector domains, the role of paired domains and how they function in a combinatorial fashion within chromatin are poorly defined. Here we show that the linked tandem Tudor and plant homeodomain (PHD) of UHRF1 (ubiquitin-like PHD and RING finger domain-containing protein 1) operates as a functional unit in cells, providing a defined combinatorial readout of a heterochromatin signature within a single histone H3 tail that is essential for UHRF1-directed epigenetic inheritance of DNA methylation. These findings provide critical support for the "histone code" hypothesis, demonstrating that multivalent histone engagement plays a key role in driving a fundamental downstream biological event in chromatin.
journal_name
Genes Devjournal_title
Genes & developmentauthors
Rothbart SB,Dickson BM,Ong MS,Krajewski K,Houliston S,Kireev DB,Arrowsmith CH,Strahl BDdoi
10.1101/gad.220467.113subject
Has Abstractpub_date
2013-06-01 00:00:00pages
1288-98issue
11eissn
0890-9369issn
1549-5477pii
27/11/1288journal_volume
27pub_type
杂志文章abstract：:The rpoH gene of Escherichia coli encodes sigma 32, the 32-kD sigma-factor responsible for the heat-inducible transcription of the heat shock genes. rpoH is transcribed from at least three promoters. Two of these promoters are recognized by RNA polymerase containing sigma 70, the predominant sigma-factor. We purified ...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.3.9.1462
更新日期：1989-09-01 00:00:00
abstract：:The YAP transcription coactivator has been implicated as an oncogene and is amplified in human cancers. Recent studies have established that YAP is phosphorylated and inhibited by the Hippo tumor suppressor pathway. Here we demonstrate that the TEAD family transcription factors are essential in mediating YAP-dependent...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.1664408
更新日期：2008-07-15 00:00:00
abstract：:DNA replication remains unfinished in many Drosophila polyploid cells, which harbor disproportionately fewer copies of late-replicating chromosomal regions. By analyzing paired-end high-throughput sequence data from polytene larval salivary gland cells, we define 112 underreplicated (UR) euchromatic regions 60-480 kb ...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.245811.114
更新日期：2014-08-15 00:00:00
abstract：:Multiple DNA repair pathways maintain genome stability and ensure that DNA remains essentially unchanged over the life of a cell. Various human diseases occur if DNA repair is compromised, and most of these impact the nervous system, in some cases exclusively. However, it is often unclear what specific endogenous dama...
journal_title：Genes & development
pub_type： 杂志文章,评审
doi：10.1101/gad.301325.117
更新日期：2017-06-15 00:00:00
abstract：:Ecsit is a cytosolic adaptor protein essential for inflammatory response and embryonic development via the Toll-like and BMP (bone morphogenetic protein) signal transduction pathways, respectively. Here, we demonstrate a mitochondrial function for Ecsit (an evolutionary conserved signaling intermediate in Toll pathway...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.408407
更新日期：2007-03-01 00:00:00
abstract：:Diverse developmental processes, such as neuronal growth cone migration and cell shape changes, are mediated by the interactions of cells with the extracellular matrix. We describe here a secreted molecule encoded by the Drosophila masquerade (mas) gene. Total loss of mas function causes defective muscle attachment. T...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.9.2.139
更新日期：1995-01-15 00:00:00
abstract：:Mediator was resolved from yeast as a multiprotein complex on the basis of its requirement for transcriptional activation in a fully defined system. Three groups of mediator polypeptides could be distinguished: the products of five SRB genes, identified as suppressors of carboxy-terminal domain (CTD)-truncation mutant...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.12.1.45
更新日期：1998-01-01 00:00:00
abstract：:TP53 is the most frequently mutated gene in human cancer. Many mutant p53 proteins exert oncogenic gain-of-function (GOF) properties that contribute to metastasis, but the mechanisms mediating these functions remain poorly defined in vivo. To elucidate how mutant p53 GOF drives metastasis, we developed a traceable som...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.304972.117
更新日期：2017-09-15 00:00:00
abstract：:Alu repetitive sequences are the most abundant short interspersed DNA elements in the human genome. Full-length Alu elements are composed of two tandem sequence monomers, the left and right Alu arms, both derived from the 7SL signal recognition particle RNA. Since Alu elements are common in protein-coding genes, they ...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.197467.112
更新日期：2012-09-01 00:00:00
abstract：:Fgf-10-deficient mice (Fgf-10(-/-)) were generated to determine the role(s) of Fgf-10 in vertebrate development. Limb bud initiation was abolished in Fgf-10(-/-) mice. Strikingly, Fgf-10(-/-) fetuses continued to develop until birth, despite the complete absence of both fore- and hindlimbs. Fgf-10 is necessary for api...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.12.20.3156
更新日期：1998-10-15 00:00:00
abstract：:Lethal yellow (Ay) is a mutation at the mouse agouti (a) locus that is associated with an all-yellow coat color, obesity, diabetes, tumors in heterozygotes, and preimplantation embryonic lethality in homozygotes. Previously, we cloned and characterized the wild-type agouti gene and demonstrated that it expresses a 0.8...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.7.7a.1203
更新日期：1993-07-01 00:00:00
abstract：:Mutations that influence lin-12 activity in Caenorhabditis elegans may identify conserved factors that regulate the activity of lin-12/Notch proteins. We describe genetic evidence indicating that sel-10 is a negative regulator of lin-12/Notch-mediated signaling in C. elegans. Sequence analysis shows that SEL-10 is a m...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.11.23.3182
更新日期：1997-12-01 00:00:00
abstract：:A core promoter element called an initiator (Inr) overlaps the transcription start site of numerous mammalian protein-coding genes. In promoters that lack a TATA box, the Inr is functionally analogous to TATA, in that it is capable of directing basal transcription by RNA polymerase II and of determining the precise si...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.8.7.821
更新日期：1994-04-01 00:00:00
abstract：:The structurally related POU homeo domain proteins Pit-1 and Oct-2 activate pituitary- and lymphoid-specific transcription, respectively, by binding to similar AT-rich motifs in their target genes. In this study we identify bases critical for recognition and activation by Pit-1 and examine how small differences in Pit...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.4.1.43
更新日期：1990-01-01 00:00:00
abstract：:Although progesterone has been recognized as essential for the establishment and maintenance of pregnancy, this steroid hormone has been recently implicated to have a functional role in a number of other reproductive events. The physiological effects of progesterone are mediated by the progesterone receptor (PR), a me...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.9.18.2266
更新日期：1995-09-15 00:00:00
abstract：:Transcription of frog rDNA by mouse cell factors is the only documented exception to the observed species selectivity of rRNA gene expression. This heterologous transcription is authentic in that it uses the normal frog upstream and core promoter domains, as well as the normal mouse polymerase I transcription factors,...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.4.1.52
更新日期：1990-01-01 00:00:00
abstract：:A novel system to study early hematopoietic development is described. This report documents the in vitro capacity of murine embryonic stem (ES) cells to differentiate into hematopoietic precursors of most, if not all, of the colony-forming cells found in normal bone marrow. This system is used to correlate the genetic...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.5.5.728
更新日期：1991-05-01 00:00:00
abstract：:We investigated the regulation of the activin/nodal-inducible distal element (DE) of the Xenopus goosecoid (gsc) promoter. On the basis of its interaction with the DE, we isolated a Xenopus homolog of the human Williams-Beuren syndrome critical region 11 (XWBSCR11), and further, show that it interacts with pathway-spe...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.963802
更新日期：2002-04-01 00:00:00
abstract：:The Saccharomyces cerevisiae protein kinase Rim15p was identified previously as a stimulator of meiotic gene expression. Here, we show that loss of Rim15p causes an additional pleiotropic phenotype in cells grown to stationary phase on rich medium; this phenotype includes defects in trehalose and glycogen accumulation...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.12.18.2943
更新日期：1998-09-15 00:00:00
abstract：:Cdc42p, a Rho-related GTP-binding protein, regulates cytoskeletal polarization and rearrangements in eukaryotic cells, but the effectors mediating this control remain unknown. Through the use of the complete yeast genomic sequence, we have identified two novel Cdc42p targets, Gic1p and Gic2p, which contain consensus C...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.11.22.2972
更新日期：1997-11-15 00:00:00
abstract：:The N-myc gene encodes a putative transcription factor that is thought to function in the regulation of gene expression during cell differentiation and/or growth. To examine the role of N-myc during development, we have used targeted mutagenesis in embryonic stem cells to produce a mouse line that carries an N-myc nul...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.6.12a.2248
更新日期：1992-12-01 00:00:00
abstract：:Ectopic repression of retinoic acid (RA) receptor target genes by PML/RARA and PLZF/RARA fusion proteins through aberrant recruitment of nuclear corepressor complexes drives cellular transformation and acute promyelocytic leukemia (APL) development. In the case of PML/RARA, this repression can be reversed through trea...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.512009
更新日期：2009-05-15 00:00:00
abstract：:Rb mutants exhibit aneuploidy and aberrant chromosome structure during mitosis. In this issue of Genes & Development, a new paper from Longworth and colleagues (1011-1024) describes both physical and functional interactions between Drosophila Rbf1 and the dCAP-D3 subunit of condensin II. This work directly implicates ...
journal_title：Genes & development
pub_type： 评论,杂志文章
doi：10.1101/gad.1666808
更新日期：2008-04-15 00:00:00
abstract：:Riboswitches are RNA elements that undergo a shift in structure in response to binding of a regulatory molecule. These elements are encoded within the transcript they regulate, and act in cis to control expression of the coding sequence(s) within that transcript; their function is therefore distinct from that of small...
journal_title：Genes & development
pub_type： 杂志文章,评审
doi：10.1101/gad.1747308
更新日期：2008-12-15 00:00:00
abstract：:In vivo experiments have demonstrated that the ribosomal protein L32 of Saccharomyces cerevisiae brings about the inhibition of splicing of the transcript of its own gene through an RNA structure comprised largely of the first exon. We now show that L32, itself, binds specifically to this RNA. Splicing of the RPL32 tr...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.8.2.211
更新日期：1994-01-01 00:00:00
abstract：:Recruitment of p300/CBP by the hypoxia-inducible factor, HIF-1, is essential for the transcriptional response to hypoxia and requires an interaction between the p300/CBP CH1 region and HIF-1alpha. A new p300-CH1 interacting protein, p35srj, has been identified and cloned. p35srj is an alternatively spliced isoform of ...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.13.1.64
更新日期：1999-01-01 00:00:00
abstract：:The neurogenic loci of Drosophila are required for proper partitioning of ectodermal cells into epidermal versus neural lineages. The loci appear to encode components of a developmental pathway involving cellular communication. In an effort to understand the role of the neurogenic locus mastermind in these processes, ...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.4.10.1688
更新日期：1990-10-01 00:00:00
abstract：:The recombination site dif is the target on the Escherichia coli chromosome of the site-specific recombinases XerC and XerD. The dif/XerC-D system plays a role during the cell cycle, probably by favoring sister chromosome monomerization or separation. A phenomenon of regional control over dif activity, also analyzed i...
journal_title：Genes & development
pub_type： 评论,杂志文章
doi：10.1101/gad.10.9.1152
更新日期：1996-05-01 00:00:00
abstract：:Estrogen receptor α (ER) is the major driver of ∼75% of breast cancers, and multiple ER targeting drugs are routinely used clinically to treat patients with ER+ breast cancer. However, many patients relapse on these targeted therapies and ultimately develop metastatic and incurable disease, and understanding the mecha...
journal_title：Genes & development
pub_type： 杂志文章,评审
doi：10.1101/gad.316646.118
更新日期：2018-09-01 00:00:00
abstract：:Reactive oxygen species (ROS) participate in numerous cell responses, including proliferation, DNA damage, and cell death. Based on these disparate activities, both promotion and inhibition of ROS have been proposed for cancer therapy. However, how the ROS response is determined is not clear. We examined the activitie...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.271130.115
更新日期：2016-01-01 00:00:00
