Identification of the sigma E subunit of Escherichia coli RNA polymerase: a second alternate sigma factor involved in high-temperature gene expression.

abstract：:Although the transporter-like protein Patched (Ptc) is genetically implicated in reception of the extracellular Hedgehog (Hh) protein signal, a clear definition of the Hh receptor is complicated by the existence of additional Hh-binding proteins and, in Drosophila, by the lack of physical evidence for direct binding o...

journal_title：Genes & development

authors： Zheng X,Mann RK,Sever N,Beachy PA

更新日期：2010-01-01 00:00:00

abstract：:The chromo domain was identified as a homologous protein motif between Polycomb (Pc)--a member of the Pc-group genes encoding transcriptional repressors of the homeotic genes--and HP1--a heterochromatin-associated protein encoded by the suppressor of position effect variegation gene Su(var)205. Together with previous ...

journal_title：Genes & development

authors： Messmer S,Franke A,Paro R

更新日期：1992-07-01 00:00:00

abstract：:We have investigated the role of erythroid Kruppel-like factor (EKLF) in expression of the human beta-globin genes in compound EKLF knockout/human beta-locus transgenic mice. EKLF affects only the adult mouse beta-globin genes in homozygous knockout mice; heterozygous mice are unaffected. Here we show that EKLF knocko...

journal_title：Genes & development

authors： Wijgerde M,Gribnau J,Trimborn T,Nuez B,Philipsen S,Grosveld F,Fraser P

更新日期：1996-11-15 00:00:00

abstract：:Protein kinases are key regulators of cellular processes. In spite of considerable effort, a full understanding of the pathways they participate in remains elusive. We globally investigated the proteins that interact with the majority of yeast protein kinases using protein microarrays. Eighty-five kinases were purifie...

journal_title：Genes & development

authors： Fasolo J,Sboner A,Sun MG,Yu H,Chen R,Sharon D,Kim PM,Gerstein M,Snyder M

更新日期：2011-04-01 00:00:00

abstract：:By studying neuroectoderm formation in the absence of mesoderm and mesectoderm in mutants of the zygotic genes snail and twist, we have found that the number of neuroblasts is not reduced in these mutants, suggesting that mesoderm and mesectoderm are not essential for the initiation of neural development. The position...

journal_title：Genes & development

authors： Rao Y,Vaessin H,Jan LY,Jan YN

更新日期：1991-09-01 00:00:00

abstract：:Class Ia phosphatidylinositol 3 kinase (PI3K) is required for oncogenic receptor-mediated transformation; however, the individual roles of the two commonly expressed class Ia PI3K isoforms in oncogenic receptor signaling have not been elucidated in vivo. Here, we show that genetic ablation of p110α blocks tumor format...

journal_title：Genes & development

authors： Utermark T,Rao T,Cheng H,Wang Q,Lee SH,Wang ZC,Iglehart JD,Roberts TM,Muller WJ,Zhao JJ

更新日期：2012-07-15 00:00:00

abstract：:Myxococcus xanthus fruiting body development is induced by amino acid limitation. The decision to grow or develop is established by the RelA-dependent stringent response and A-signaling. We identified two new members of this regulatory hierarchy, socE and the C-signaling gene csgA. SocE depletion arrests growth and in...

journal_title：Genes & development

authors： Crawford EW Jr,Shimkets LJ

更新日期：2000-02-15 00:00:00

abstract：:Using an in vitro system in which a 5' splice site (5'SS) RNA oligo (AAG decreases GUAAGUAdT) is capable of inducing formation of U2/U4/U5/U6 snRNP complex we show that this oligo specifically binds to U4/U5/U6 snRNP and cross-links to U6 snRNA in the absence of U2 snRNP. Moreover, 5'SS RNA oligo bound to U4/U5/U6 snR...

journal_title：Genes & development

authors： Konforti BB,Konarska MM

更新日期：1994-08-15 00:00:00

abstract：:Homeotic genes are expressed in spatially precise patterns during Drosophila development to control segmental differentiation. The Sex combs reduced (Scr) gene of the Antennapedia gene complex is involved in the determination of the labial and prothoracic segments of the embryo. To study both the wild-type pattern of ...

journal_title：Genes & development

authors： Riley PD,Carroll SB,Scott MP

更新日期：1987-09-01 00:00:00

abstract：:In the yeast Saccharomyces cerevisiae, sequence-specific DNA binding by the origin recognition complex (ORC) is responsible for selecting origins of DNA replication. In metazoans, origin selection is poorly understood and it is unknown whether specific DNA binding by metazoan ORC controls replication. To address this ...

journal_title：Genes & development

authors： Austin RJ,Orr-Weaver TL,Bell SP

更新日期：1999-10-15 00:00:00

abstract：:Interruption of translation in Escherichia coli can lead to the addition of an 11-residue carboxy-terminal peptide tail to the nascent chain. This modification is mediated by SsrA RNA (also called 10Sa RNA and tmRNA) and marks the tagged polypeptide for proteolysis. Degradation in vivo of lambda repressor amino-termin...

journal_title：Genes & development

authors： Gottesman S,Roche E,Zhou Y,Sauer RT

更新日期：1998-05-01 00:00:00

abstract：:Medulloblastomas are among the most common malignancies in childhood, and they are associated with substantial mortality and morbidity. The molecular pathogenesis as well as the ontogeny of these neoplasms is still poorly understood. We have generated a mouse model for medulloblastoma by Cre-LoxP-mediated inactivation...

journal_title：Genes & development

authors： Marino S,Vooijs M,van Der Gulden H,Jonkers J,Berns A

更新日期：2000-04-15 00:00:00

abstract：:The 134-bp enhancer region of the pancreatic elastase I gene is sufficient to direct pancreatic acinar cell-specific transcription in transgenic mice and in transfected cells in culture. Ten-base-pair scanner mutations in three separate enhancer domains that inactivate enhancer function in transfected pancreatic cells...

journal_title：Genes & development

authors： Swift GH,Kruse F,MacDonald RJ,Hammer RE

更新日期：1989-05-01 00:00:00

abstract：:Transcription by RNA polymerase II (RNAPII) is a dynamic process with frequent variations in the elongation rate. However, the physiological relevance of variations in RNAPII elongation kinetics has remained unclear. Here we show in yeast that a RNAPII mutant that reduces the transcription elongation rate causes wides...

journal_title：Genes & development

authors： Yague-Sanz C,Vanrobaeys Y,Fernandez R,Duval M,Larochelle M,Beaudoin J,Berro J,Labbé S,Jacques PÉ,Bachand F

更新日期：2020-07-01 00:00:00

abstract：:Sonic hedgehog (Shh) signaling plays a critical role during development and carcinogenesis. While Gli family members govern the transcriptional output of Shh signaling, little is known how Gli-mediated transcriptional activity is regulated. Here we identify the actin-binding protein Missing in Metastasis (MIM) as a ne...

journal_title：Genes & development

authors： Callahan CA,Ofstad T,Horng L,Wang JK,Zhen HH,Coulombe PA,Oro AE

更新日期：2004-11-15 00:00:00

abstract：:Joints form within the developing skeleton through the segmentation and cavitation of initially continuous cartilage condensations. However, the molecular pathways controlling joint formation largely remain to be clarified. In particular, while several critical secreted signals have been identified, no transcription f...

journal_title：Genes & development

authors： Kan A,Tabin CJ

更新日期：2013-03-01 00:00:00

abstract：:Cell cycle checkpoints are critical for genomic stability. Rad17, a component of the checkpoint clamp loader complex (Rad17/Rfc2-5), is required for the response to DNA damage and replication stress. To explore the role of Rad17 in the maintenance of genomic integrity, we established somatic conditional alleles of RAD...

journal_title：Genes & development

authors： Wang X,Zou L,Zheng H,Wei Q,Elledge SJ,Li L

更新日期：2003-04-15 00:00:00

abstract：:During vascular development, endothelial platelet-derived growth factor B (PDGF-B) is critical for pericyte recruitment. Deletion of the conserved C-terminal heparin-binding motif impairs PDGF-BB retention and pericyte recruitment in vivo, suggesting a potential role for heparan sulfate (HS) in PDGF-BB function during...

journal_title：Genes & development

authors： Abramsson A,Kurup S,Busse M,Yamada S,Lindblom P,Schallmeiner E,Stenzel D,Sauvaget D,Ledin J,Ringvall M,Landegren U,Kjellén L,Bondjers G,Li JP,Lindahl U,Spillmann D,Betsholtz C,Gerhardt H

更新日期：2007-02-01 00:00:00

abstract：:Covalent modifications of the histone N tails play important roles in eukaryotic gene expression. Histone acetylation, in particular, is required for the activation of a subset of eukaryotic genes through the targeted recruitment of histone acetyltransferases. We have reported that a histone C tail modification, ubiqu...

journal_title：Genes & development

authors： Kao CF,Hillyer C,Tsukuda T,Henry K,Berger S,Osley MA

更新日期：2004-01-15 00:00:00

abstract：:The HOP2 and MND1 genes are indispensable for meiotic recombination. The products of these genes associate to form a stable heterodimeric complex that binds DNA and stimulates the recombinase activity of Rad51 and Dmc1. Here we conduct molecular studies to delineate the action mechanism of the Hop2-Mnd1 complex. We pr...

journal_title：Genes & development

authors： Chi P,San Filippo J,Sehorn MG,Petukhova GV,Sung P

更新日期：2007-07-15 00:00:00

abstract：:Genomic imprinting is an epigenetic phenomenon leading to parentally biased gene expression. Throughout the years, extensive efforts have been made to characterize the epigenetic marks underlying imprinting in animals and plants. As a result, DNA methylation asymmetries between parental genomes emerged as the primary ...

journal_title：Genes & development

authors： Batista RA,Köhler C

更新日期：2020-01-01 00:00:00

abstract：:The RNA polymerase II (RNAPII) C-terminal domain kinase, CDK12, regulates genome stability, expression of DNA repair genes, and cancer cell resistance to chemotherapy and immunotherapy. In addition to its role in mRNA biosynthesis of DNA repair genes, we show here that CDK12 phosphorylates the mRNA 5' cap-binding repr...

journal_title：Genes & development

authors： Choi SH,Martinez TF,Kim S,Donaldson C,Shokhirev MN,Saghatelian A,Jones KA

更新日期：2019-04-01 00:00:00

abstract：:The human Y-family DNA polymerases, Poliota, Poleta, and Polkappa, function in promoting replication through DNA lesions. However, because of their low fidelity, any involvement of these polymerases in DNA synthesis during base excision repair (BER) would be highly mutagenic. Mechanisms, therefore, must exist to exclu...

journal_title：Genes & development

authors： Haracska L,Prakash L,Prakash S

更新日期：2003-11-15 00:00:00

abstract：:P-element transformants of a single rRNA gene (rDNA) were used to investigate the relationship between the organization of the nucleolus organizer (NO) and rDNA function in Drosophila melanogaster. In situ hybridization to rRNA in polytene nuclei of salivary glands demonstrated that an rRNA gene can be transcribed at ...

journal_title：Genes & development

authors： Karpen GH,Schaefer JE,Laird CD

更新日期：1988-12-01 00:00:00

abstract：:Disruptions in the use of skeletal muscle lead to muscle atrophy. After short periods of disuse, muscle atrophy is reversible, and even after prolonged periods of inactivity, myofiber degeneration is uncommon. The pathways that regulate atrophy, initiated either by peripheral nerve damage, immobilization, aging, catab...

journal_title：Genes & development

authors： Wang X,Blagden C,Fan J,Nowak SJ,Taniuchi I,Littman DR,Burden SJ

更新日期：2005-07-15 00:00:00

abstract：:Extensive DNA elimination via a DNA breakage and rejoining process occurs during macronuclear development in the hypotrich ciliate Euplotes crassus. The excision process involves the removal of short, unique segments of DNA (internal eliminated sequences; IESs) and at least two highly repetitive families of transposon...

journal_title：Genes & development

authors： Klobutcher LA,Turner LR,LaPlante J

更新日期：1993-01-01 00:00:00

abstract：:In many eukaryotes, siRNAs bound to Argonaute proteins guide chromatin-modifying enzymes to complementary loci, resulting in transcriptional gene silencing. Multiple lines of evidence indicate that siRNAs base-pair with longer RNAs produced at target loci, but the possibility that siRNAs base-pair directly with DNA re...

journal_title：Genes & development

authors： Wendte JM,Pikaard CS

更新日期：2016-12-15 00:00:00

abstract：:Histone post-translational modifications regulate chromatin structure and function largely through interactions with effector proteins that often contain multiple histone-binding domains. While significant progress has been made characterizing individual effector domains, the role of paired domains and how they functi...

journal_title：Genes & development

authors： Rothbart SB,Dickson BM,Ong MS,Krajewski K,Houliston S,Kireev DB,Arrowsmith CH,Strahl BD

更新日期：2013-06-01 00:00:00

abstract：:DNA repair associated with DNA replication is important for the conservation of genomic sequence information, whereas reconstitution of chromatin after replication sustains epigenetic information. We have isolated and characterized mutations in the BRU1 gene of Arabidopsis that suggest a novel link between these under...

journal_title：Genes & development

authors： Takeda S,Tadele Z,Hofmann I,Probst AV,Angelis KJ,Kaya H,Araki T,Mengiste T,Mittelsten Scheid O,Shibahara K,Scheel D,Paszkowski J

更新日期：2004-04-01 00:00:00

abstract：:We have identified protein kinase C-zeta (PKC-zeta) as a novel suppressor of neoplastic transformation caused by the v-raf oncogene. PKC-zeta overexpression drastically retards proliferation, abolishes anchorage-independent growth, and reverts the morphological transformation of v-raf-transformed NIH-3T3 cells. The mo...

journal_title：Genes & development

authors： Kieser A,Seitz T,Adler HS,Coffer P,Kremmer E,Crespo P,Gutkind JS,Henderson DW,Mushinski JF,Kolch W,Mischak H

更新日期：1996-06-15 00:00:00