Abstract:
:During vascular development, endothelial platelet-derived growth factor B (PDGF-B) is critical for pericyte recruitment. Deletion of the conserved C-terminal heparin-binding motif impairs PDGF-BB retention and pericyte recruitment in vivo, suggesting a potential role for heparan sulfate (HS) in PDGF-BB function during vascular development. We studied the participation of HS chains in pericyte recruitment using two mouse models with altered HS biosynthesis. Reduction of N-sulfation due to deficiency in N-deacetylase/N-sulfotransferase-1 attenuated PDGF-BB binding in vitro, and led to pericyte detachment and delayed pericyte migration in vivo. Reduced N-sulfation also impaired PDGF-BB signaling and directed cell migration, but not proliferation. In contrast, HS from glucuronyl C5-epimerase mutants, which is extensively N- and 6-O-sulfated, but lacks 2-O-sulfated L-iduronic acid residues, retained PDGF-BB in vitro, and pericyte recruitment in vivo was only transiently delayed. These observations were supported by in vitro characterization of the structural features in HS important for PDGF-BB binding. We conclude that pericyte recruitment requires HS with sufficiently extended and appropriately spaced N-sulfated domains to retain PDGF-BB and activate PDGF receptor beta (PDGFRbeta) signaling, whereas the detailed sequence of monosaccharide and sulfate residues does not appear to be important for this interaction.
journal_name
Genes Devjournal_title
Genes & developmentauthors
Abramsson A,Kurup S,Busse M,Yamada S,Lindblom P,Schallmeiner E,Stenzel D,Sauvaget D,Ledin J,Ringvall M,Landegren U,Kjellén L,Bondjers G,Li JP,Lindahl U,Spillmann D,Betsholtz C,Gerhardt Hdoi
10.1101/gad.398207subject
Has Abstractpub_date
2007-02-01 00:00:00pages
316-31issue
3eissn
0890-9369issn
1549-5477pii
21/3/316journal_volume
21pub_type
杂志文章abstract::Cyclin D1 is part of a cell cycle control node consistently deregulated in most human cancers. However, studies with cyclin D1-null mice indicate that it is dispensable for normal mouse development as well as cell growth in culture. Here, we provide evidence that ras-mediated tumorigenesis depends on signaling pathway...
journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.12.16.2469
更新日期:1998-08-15 00:00:00
abstract::Class Ia phosphatidylinositol 3 kinase (PI3K) is required for oncogenic receptor-mediated transformation; however, the individual roles of the two commonly expressed class Ia PI3K isoforms in oncogenic receptor signaling have not been elucidated in vivo. Here, we show that genetic ablation of p110α blocks tumor format...
journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.191973.112
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abstract::Programmed cell death occurs in a highly reproducible manner during Caenorhabditis elegans development. We demonstrate that, during embryogenesis, miR-35 and miR-58 bantam family microRNAs (miRNAs) cooperate to prevent the precocious death of mothers of cells programmed to die by repressing the gene egl-1, which encod...
journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.288555.116
更新日期:2017-01-15 00:00:00
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journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.2.12b.1700
更新日期:1988-12-01 00:00:00
abstract::The c-fos serum response element (SRE) is a multifunctional regulatory region of the c-fos promoter that responds to a variety of inducers. Recently, we have demonstrated that the SRE binds the C/EBP-related transcription factor rat NFIL-6 (rNFIL-6). In this study we show that rNFIL-6 is regulated by the cAMP second m...
journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.5.10.1754
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journal_title:Genes & development
pub_type: 杂志文章
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abstract::Recombination catalyzed by the Flp site-specific recombinase involves breakage and joining of four DNA strands between two target substrates. The reaction is carried out in two steps of pairwise strand exchanges by a DNA-protein assembly in which four Flp monomers act cooperatively to execute strand cleavage and joini...
journal_title:Genes & development
pub_type: 杂志文章
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abstract::The initial steps of double-stranded break (DSB) repair by homologous recombination mediated by the 5'-3' exonuclease/annealing protein pairs, RecE/RecT and Redalpha/Redbeta, were analyzed. Recombination was RecA-independent and required the expression of both components of an orthologous pair, even when the need for ...
journal_title:Genes & development
pub_type: 杂志文章
doi:
更新日期:2000-08-01 00:00:00
abstract::Directed cell motility is at the basis of biological phenomena such as development, wound healing, and metastasis. It has been shown that substrate attachments mediate motility by coupling the cell's cytoskeleton with force generation. However, it has been unclear how the persistence of cell directionality is facilita...
journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.190413.112
更新日期:2012-09-01 00:00:00
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journal_title:Genes & development
pub_type: 杂志文章
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更新日期:1993-02-01 00:00:00
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journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.455407
更新日期:2007-12-15 00:00:00
abstract::The Wnt pathway controls numerous developmental processes via the beta-catenin-TCF/LEF transcription complex. Deregulation of the pathway results in the aberrant accumulation of beta-catenin in the nucleus, often leading to cancer. Normally, cytoplasmic beta-catenin associates with APC and axin and is continuously pho...
journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.230302
更新日期:2002-05-01 00:00:00
abstract::Embryonic stem (ES) cells are pluripotent cells with the ability to self-renew indefinitely. These unique properties are controlled by genetic factors and chromatin structure. The exit from the self-renewing state is accompanied by changes in epigenetic chromatin modifications such as an induction in the silencing-ass...
journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.1588207
更新日期:2007-10-15 00:00:00
abstract::TGF-β receptors phosphorylate SMAD2 and SMAD3 transcription factors, which then form heterotrimeric complexes with SMAD4 and cooperate with context-specific transcription factors to activate target genes. Here we provide biochemical and structural evidence showing that binding of SMAD2 to DNA depends on the conformati...
journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.330837.119
更新日期:2019-11-01 00:00:00
abstract::Epigenetic modifications of chromatin play an important role in the regulation of gene expression. KMT4/Dot1 is a conserved histone methyltransferase capable of methylating chromatin on Lys79 of histone H3 (H3K79). Here we report the identification of a multisubunit Dot1 complex (DotCom), which includes several of the...
journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.1898410
更新日期:2010-03-15 00:00:00
abstract::Eukaryotic RNAs with premature termination codons (PTCs) are eliminated by nonsense-mediated decay (NMD). While human nonsense RNA degradation can be initiated either by an endonucleolytic cleavage event near the PTC or through decapping, the individual contribution of these activities on endogenous substrates has rem...
journal_title:Genes & development
pub_type: 杂志文章
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更新日期:2014-11-15 00:00:00
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journal_title:Genes & development
pub_type: 评论,杂志文章
doi:10.1101/gad.2080411
更新日期:2011-07-15 00:00:00
abstract::Post-translational processes are essential for the generation and dynamics of mammalian circadian rhythms. In particular, phosphorylation of the key circadian protein PER2 precisely controls the period and phase of circadian oscillations. However, the mechanisms underlying that control are poorly understood. Here, we ...
journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.512209
更新日期:2009-03-15 00:00:00
abstract::Genomic imprinting, an inherently epigenetic phenomenon defined by parent of origin-dependent gene expression, is observed in mammals and flowering plants. Genome-scale surveys of imprinted expression and the underlying differential epigenetic marks have led to the discovery of hundreds of imprinted plant genes and co...
journal_title:Genes & development
pub_type: 杂志文章,评审
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更新日期:2015-12-15 00:00:00
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journal_title:Genes & development
pub_type: 杂志文章
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journal_title:Genes & development
pub_type: 杂志文章
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journal_title:Genes & development
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更新日期:2013-01-15 00:00:00
abstract::Telomeres protect the ends of linear chromosomes from degradation and abnormal recombination events, and in vertebrates may be important in cellular senescence and cancer. However, very little is known about the structure of human telomeres. In this report we purify telomeres and analyze their termini. We show that fo...
journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.11.21.2801
更新日期:1997-11-01 00:00:00
abstract::The subdivision of the Drosophila body into distinct terminal and central domains depends on the torso (tor) protein, a putative receptor tyrosine kinase that is active at both ends of the early embryo. We show that the tor protein is uniformly expressed along the surface membrane of early embryos despite its localize...
journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.3.12b.2025
更新日期:1989-12-01 00:00:00
abstract::The ascidian tadpole represents the most simplified chordate body plan. It contains a notochord composed of just 40 cells, but as in vertebrates Brachyury is essential for notochord differentiation. Here, we show that the misexpression of the Brachyury gene (Ci-Bra) of Ciona intestinalis is sufficient to transform end...
journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.13.12.1519
更新日期:1999-06-15 00:00:00
abstract::Drosophila melanogaster neural-specific protein, ELAV, has been shown to regulate the neural-specific splicing of three genes: neuroglian (nrg), erect wing, and armadillo. Alternative splicing of the nrg transcript involves alternative inclusion of a 3'-terminal exon. Here, using a minigene reporter, we show that the ...
journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.903101
更新日期:2001-10-01 00:00:00
abstract::Accurate completion of replication relies on the ability of cells to activate error-free recombination-mediated DNA damage bypass at sites of perturbed replication. However, as anti-recombinase activities are also recruited to replication forks, how recombination-mediated damage bypass is enabled at replication stress...
journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.265629.115
更新日期:2015-10-01 00:00:00
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journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.1970910
更新日期:2010-11-15 00:00:00
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journal_title:Genes & development
pub_type: 杂志文章
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更新日期:1989-01-01 00:00:00
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journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.1998811
更新日期:2011-04-01 00:00:00