Abstract：
:Although the transporter-like protein Patched (Ptc) is genetically implicated in reception of the extracellular Hedgehog (Hh) protein signal, a clear definition of the Hh receptor is complicated by the existence of additional Hh-binding proteins and, in Drosophila, by the lack of physical evidence for direct binding of Hh to Ptc. Here we show that activity of Ihog (Interference hedgehog), or of its close relative Boi (Brother of Ihog), is absolutely required for Hh biological response and for sequestration of the Hh protein to limit long-range signaling. We demonstrate that Ihog interacts directly with Ptc, is required for presentation of Ptc on the cell surface, and that Ihog and Ptc are both required for high-affinity Hh binding. On the basis of their joint roles in ligand binding, signal transduction, and receptor trafficking, we conclude that Ihog and Ptc together constitute the Drosophila Hh receptor.
journal_name
Genes Devjournal_title
Genes & developmentauthors
Zheng X,Mann RK,Sever N,Beachy PAdoi
10.1101/gad.1870310subject
Has Abstractpub_date
2010-01-01 00:00:00pages
57-71issue
1eissn
0890-9369issn
1549-5477pii
24/1/57journal_volume
24pub_type
杂志文章abstract：:Lipids play crucial roles in many aspects of glial cell biology, affecting processes ranging from myelin membrane biosynthesis to axo-glial interactions. In order to study the role of lipid metabolism in myelinating glial cells, we specifically deleted in Schwann cells the Lpin1 gene, which encodes the Mg2+-dependent ...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.1638008
更新日期：2008-06-15 00:00:00
abstract：:The hair follicle undergoes a lifelong developmental cycle that depends on the integration between activating and inhibitory signals acting to regulate and guide the proliferation and differentiation of pluripotent epithelial stem cells. The effectors and mechanisms responsible for re-entry of quiescent telogen hair f...
journal_title：Genes & development
pub_type： 评论,杂志文章
doi：10.1101/gad.223172.113
更新日期：2013-06-15 00:00:00
abstract：:Sexual identity in Drosophila is determined by zygotic X-chromosome dose. Two potent indicators of X-chromosome dose are sisterless-a (sis-a) and sisterless-b (sis-b). Genetic analysis has shown that a diplo-X dose of these genes activates their regulatory target, the feminizing switch gene Sex-lethal (Sxl), whereas a...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.7.9.1688
更新日期：1993-09-01 00:00:00
abstract：:The myc family of cellular oncogenes encodes three highly related nuclear phosphoproteins (c-Myc, N-Myc, and L-Myc) that are believed to function as sequence-specific transcription factors capable of regulating genes important in cellular growth and differentiation. Current evidence indicates that Myc family proteins ...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.6.8.1480
更新日期：1992-08-01 00:00:00
abstract：:The Swi/Snf chromatin remodeling complex functions to alter nucleosome positions by either sliding nucleosomes on DNA or the eviction of histones. The presence of histone acetylation and activator-dependent recruitment and retention of Swi/Snf is important for its efficient function. It is not understood, however, why...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.243584.114
更新日期：2014-10-15 00:00:00
abstract：:In enteric bacteria, the transcription factor σ(E) maintains membrane homeostasis by inducing synthesis of proteins involved in membrane repair and two small regulatory RNAs (sRNAs) that down-regulate synthesis of abundant membrane porins. Here, we describe the discovery of a third σ(E)-dependent sRNA, MicL (mRNA-inte...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.243485.114
更新日期：2014-07-15 00:00:00
abstract：:Most eukaryotic mRNAs are translated using a cap-dependent mechanism of translation. However, approximately 10% of mammalian mRNAs initiate translation using a cap-independent mechanism that is not well understood. These mRNAs contain an internal ribosome entry site (IRES) located in the 5' untranslated region. The cr...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.1832209
更新日期：2009-12-01 00:00:00
abstract：:AMP-activated protein kinase (AMPK) is a sensor of energy status that maintains cellular energy homeostasis. It arose very early during eukaryotic evolution, and its ancestral role may have been in the response to starvation. Recent work shows that the kinase is activated by increases not only in AMP, but also in ADP....
journal_title：Genes & development
pub_type： 杂志文章,评审
doi：10.1101/gad.17420111
更新日期：2011-09-15 00:00:00
abstract：:Medulloblastomas are brain tumors that arise in the cerebellum of children and contain stem cells in a perivascular niche thought to give rise to recurrence following radiation. We used several mouse models of medulloblastomas in parallel to better understand how the critical cell types in these tumors respond to ther...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.1627008
更新日期：2008-02-15 00:00:00
abstract：:The Drosophila external sensory organ forms in a lineage elaborating from a single precursor cell via a stereotypical series of asymmetric divisions. HAMLET transcription factor expression demarcates the lineage branch that generates two internal cell types, the external sensory neuron and thecogen. In HAMLET mutant o...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.1170904
更新日期：2004-03-15 00:00:00
abstract：:Two retrovirus promoter trap vectors (U3His and U3Neo) have been used to disrupt genes expressed in totipotent murine embryonal stem (ES) cells. Selection in L-histidinol or G418 produced clones in which the coding sequences for histidinol-dehydrogenase or neomycin-phosphotransferase were fused to sequences in or near...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.6.6.919
更新日期：1992-06-01 00:00:00
abstract：:When the orphan nuclear receptors TR2 and TR4, the DNA-binding subunits of the DRED repressor complex, are forcibly expressed in erythroid cells of transgenic mice, embryos exhibit a transient mid-gestational anemia as a consequence of a reduction in the number of primitive erythroid cells. GATA-1 mRNA is specifically...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.1593307
更新日期：2007-11-01 00:00:00
abstract：:The allosteric and torpedo models have been used for 30 yr to explain how transcription terminates on protein-coding genes. The former invokes termination via conformational changes in the transcription complex and the latter proposes that degradation of the downstream product of poly(A) signal (PAS) processing is imp...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.332833.119
更新日期：2020-01-01 00:00:00
abstract：:Numerous lines of study have suggested that the Hox genes, encoding putative transcription factors, are key genes in the establishment of the body plan of the mammalian embryo. To examine the role of Hoxa-5 (Hox1.3) gene during development, we have used targeted mutagenesis in embryonic stem cells to produce a strain ...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.7.11.2085
更新日期：1993-11-01 00:00:00
abstract：:YAP (Yes-associated protein) and TAZ (transcriptional coactivator with PDZ-binding motif) are major downstream effectors of the Hippo pathway that influences tissue homeostasis, organ size, and cancer development. Aberrant hyperactivation of YAP/TAZ causes tissue overgrowth and tumorigenesis, whereas their inactivatio...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.262816.115
更新日期：2015-06-15 00:00:00
abstract：:TDP-43 is a critical RNA-binding factor associated with pre-mRNA splicing in mammals. Its expression is tightly autoregulated, with loss of this regulation implicated in human neuropathology. We demonstrate that TDP-43 overexpression in humans and mice activates a 3' untranslated region (UTR) intron, resulting in exci...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.194829.112
更新日期：2012-08-01 00:00:00
abstract：:We have analyzed pre-mRNA splicing in yeast extracts immunodepleted of the PRP18 protein. We find that while the first step of splicing (cleavage at the 5' splice site, and generation of the exon 1 and lariat intermediates) is unaffected by the absence of PRP18, the second step of splicing (excision of the lariat intr...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.7.2.320
更新日期：1993-02-01 00:00:00
abstract：:The basic helix-loop-helix (bHLH) gene Hes7, a putative Notch effector, encodes a transcriptional repressor. Here, we found that Hes7 expression oscillates in 2-h cycles in the presomitic mesoderm (PSM). In Hes7-null mice, somites are not properly segmented and their anterior-posterior polarity is disrupted. As a resu...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.930601
更新日期：2001-10-15 00:00:00
abstract：:MicroRNAs (miRNAs) are approximately 22-nucleotide RNAs that are processed from characteristic precursor hairpins and pair to sites in messages of protein-coding genes to direct post-transcriptional repression. Here, we report that the miRNA iab-4 locus in the Drosophila Hox cluster is transcribed convergently from bo...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.1613108
更新日期：2008-01-01 00:00:00
abstract：:We have investigated the DNA-binding, oligomerization, and trans-activation functions of isolated segments of murine p53. We find that p53 has two autonomous DNA-binding regions. One domain, from amino acid 280 to 390, forms stable tetramers and binds DNA nonspecifically. The biological significance, if any, of this D...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.7.12b.2575
更新日期：1993-12-01 00:00:00
abstract：:The incretin hormone GLP1 promotes islet-cell survival via the second messenger cAMP. Here we show that mice deficient in the activity of CREB, caused by expression of a dominant-negative A-CREB transgene in pancreatic beta-cells, develop diabetes secondary to beta-cell apoptosis. Remarkably, A-CREB severely disrupted...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.1097103
更新日期：2003-07-01 00:00:00
abstract：:Two recent reports, including one by Reinders and colleagues (pp. 939-950) in the April 15, 2009, issue of Genes & Development, describe the construction of Arabidopsis recombinant inbred populations that maximize epigenetic rather than genetic variation. The distribution and behavior of phenotypic variation in these ...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.1824909
更新日期：2009-07-15 00:00:00
abstract：:We have shown that Wnt signals are necessary and sufficient for neural crest cells to adopt pigment cell fates. nacre, a zebrafish homolog of MITF, is required for pigment cell differentiation. We isolated a promoter region of nacre that contains Tcf/Lef binding sites, which can mediate Wnt responsiveness. This promot...
journal_title：Genes & development
pub_type： 杂志文章
doi：
更新日期：2000-01-15 00:00:00
abstract：:Chromatin structure is believed to be important for a number of cellular processes, including transcription. However, the role of nucleosomes in transcription is not well understood. We have identified the yeast histone locus HTB1-HTB1, encoding histones H2A and H2B, as a suppressor of solo delta insertion mutations t...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.2.2.150
更新日期：1988-02-01 00:00:00
abstract：:The product of the nuclear MRS2 gene, Mrs2p, is the only candidate splicing factor essential for all group II introns in mitochondria of the yeast Saccharomyces cerevisiae. It has been shown to be an integral protein of the inner mitochondrial membrane, structurally and functionally related to the bacterial CorA Mg(2+...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.201301
更新日期：2001-09-01 00:00:00
abstract：:Activation of the CLB gene cluster through the assembly of Mcm1p-Fkh2p complexes at target promoters is essential for mitotic entry and transition through M phase. We show that the activation of this mitotic transcriptional program is dependent on the recruitment of Ndd1p, a coactivator that performs its essential fun...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.1074103
更新日期：2003-07-15 00:00:00
abstract：:RNA polymerase II nascent transcripts are capped during pausing before elongation. Here we report that hSPT5, the human homolog of yeast elongation factor SPT5, interacts directly with the capping enzyme. hSPT5 stimulated capping enzyme guanylylation and mRNA capping by severalfold. Although RNA 5'-triphosphatase acti...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.13.14.1774
更新日期：1999-07-15 00:00:00
abstract：:Members of the metazoan nuclear receptor superfamily regulate gene expression programs in response to binding of cognate lipophilic ligands. Evolutionary studies using bioinformatics tools have concluded that lower eukaryotes, such as fungi, lack nuclear receptor homologs. Here we review recent discoveries suggesting ...
journal_title：Genes & development
pub_type： 杂志文章,评审
doi：10.1101/gad.1743009
更新日期：2009-02-15 00:00:00
abstract：:Nuclear pore complexes (NPCs) emerged as nuclear transport channels in eukaryotic cells ∼1.5 billion years ago. While the primary role of NPCs is to regulate nucleo-cytoplasmic transport, recent research suggests that certain NPC proteins have additionally acquired the role of affecting gene expression at the nuclear ...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.280941.116
更新日期：2016-05-15 00:00:00
abstract：:The mouse Nedd5 gene encodes a 41.5-kD GTPase similar to the Saccharomyces and Drosophila septins essential for cytokinesis. Nedd5 accumulates near the contractile ring from anaphase through telophase, and finally condenses into the midbody. Microinjection of anti-Nedd5 antibody interferes with cytokinesis, giving ris...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.11.12.1535
更新日期：1997-06-15 00:00:00
