Abstract：
:Homeotic genes are expressed in spatially precise patterns during Drosophila development to control segmental differentiation. The Sex combs reduced (Scr) gene of the Antennapedia gene complex is involved in the determination of the labial and prothoracic segments of the embryo. To study both the wild-type pattern of Scr expression and the regulatory relationships of Scr to other regulatory genes, an antibody probe that detects the Scr protein was prepared. We find that the Scr gene product is expressed in a dynamic pattern over the course of embryogenesis, beginning in the ectoderm in parasegment 2 while the germ band is elongated and extending to parasegment 3 during the completion of germ band shortening. The locations of Scr protein correlate well with the part of the embryo that are altered in Scr- mutants. After head involution occurs, Scr protein is also expressed in the ganglion corresponding to parasegment 2 of the ventral nervous system. The precise spatial expression of Scr is attained through regulation by both homeotic genes and segmentation genes. The lack of proper Antennapedia or Polycomb gene function causes ectopic Scr protein expression. Mutations in the segmentation genes fushi tarazu, hunchback, Krüppel, and giant alter the spatial pattern of Scr expression.
journal_name
Genes Devjournal_title
Genes & developmentauthors
Riley PD,Carroll SB,Scott MPdoi
10.1101/gad.1.7.716subject
Has Abstractpub_date
1987-09-01 00:00:00pages
716-30issue
7eissn
0890-9369issn
1549-5477journal_volume
1pub_type
杂志文章abstract：:Alternative splicing factor/splicing factor 2 (ASF/SF2) is the prototype of a family of nuclear proteins highly conserved throughout metazoa, the SR (serine/arginine) proteins. Based largely on in vitro studies, SR proteins have been suggested to play important roles in constitutive and alternative splicing of pre-mRN...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.10.20.2588
更新日期：1996-10-15 00:00:00
abstract：:The spectrum of tumors associated with oncogenic Ras in humans often differs from those in mice either treated with carcinogens or engineered to sporadically express oncogenic Ras, suggesting that the mechanism of Ras transformation may be different in humans. Ras stimulates primarily three main classes of effector pr...
journal_title：Genes & development
pub_type： 杂志文章,评审
doi：10.1101/gad.993902
更新日期：2002-08-15 00:00:00
abstract：:The identification of telomerase-negative HAATI (heterochromatin amplification-mediated and telomerase-independent) cells, in which telomeres are superseded by nontelomeric heterochromatin tracts, challenged the idea that canonical telomeres are essential for chromosome linearity and raised crucial questions as to how...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.311712.118
更新日期：2018-04-01 00:00:00
abstract：:Sox2 is expressed in basal epithelial cells of the tongue, with high levels in taste bud placodes, fungiform papillae, and mature taste cells, and low levels in filiform papillae. High Sox2 expression appears to lie downstream from canonical Wnt signaling. In hypomorphic Sox2(EGFP/LP) embryos, placodes form but no mat...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.1457106
更新日期：2006-10-01 00:00:00
abstract：:Genome instability is a recurring feature of tumorigenesis. Mutation in MLL2, encoding a histone methyltransferase, is a driver in numerous different cancer types, but the mechanism is unclear. Here, we present evidence that MLL2 mutation results in genome instability. Mouse cells in which MLL2 gene deletion can be in...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.275453.115
更新日期：2016-02-15 00:00:00
abstract：:Pseudoplasmodia of developing Dictyostelium are organized with anteroposterior polarity. We have isolated CAR4, the gene for a new cell-surface, G protein-linked cAMP receptor. CAR4 mRNA is initially expressed during tip elongation and continues to accumulate into culmination. CAR4 is maximally expressed in pseudoplas...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.8.17.2086
更新日期：1994-09-01 00:00:00
abstract：:AMP-activated protein kinase (AMPK) is a sensor of energy status that maintains cellular energy homeostasis. It arose very early during eukaryotic evolution, and its ancestral role may have been in the response to starvation. Recent work shows that the kinase is activated by increases not only in AMP, but also in ADP....
journal_title：Genes & development
pub_type： 杂志文章,评审
doi：10.1101/gad.17420111
更新日期：2011-09-15 00:00:00
abstract：:The E2F transcription factor is required for S phase in Drosophila. While it also triggers expression of replication genes at the G1-S transition, the relevance of this transcription is not clear because many of the induced gene products are sufficiently stable that new expression is not required for S phase. However,...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.9.12.1456
更新日期：1995-06-15 00:00:00
abstract：:Symbiotic nitrogen fixation involves the development of specialized organs called nodules within which plant photosynthates are exchanged for combined nitrogen of bacterial origin. To determine the importance of bacterial nitrogen metabolism in symbiosis, we have characterized a key regulator of this metabolism in Rhi...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.11.9.1194
更新日期：1997-05-01 00:00:00
abstract：:Caenorhabditis elegans GLD-1, a KH motif containing RNA-binding protein of the GSG/STAR subfamily, controls diverse aspects of germ line development, suggesting that it may have multiple mRNA targets. We used an immunoprecipitation/subtractive hybridization/cloning strategy to identify 15 mRNAs that are putative targe...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.915901
更新日期：2001-09-15 00:00:00
abstract：:Here we show that the cell cycle defects of dE2F1-depleted cells depend on the cooperative effects of dE2F2 and DACAPO (DAP), an inhibitor of Cyclin E/cyclin-dependent kinase 2 (CycE/cdk2). The different properties of cells lacking dE2F1/dE2F2 and dE2F1/DAP lead to the surprising observation that dE2F2-mediated repres...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.1031803
更新日期：2003-03-15 00:00:00
abstract：:How muscle diversity is generated in the vertebrate body is poorly understood. In the limb, dorsal and ventral muscle masses constitute the first myogenic diversification, as each gives rise to distinct muscles. Myogenesis initiates after muscle precursor cells (MPCs) have migrated from the somites to the limb bud and...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.187807.112
更新日期：2012-09-15 00:00:00
abstract：:Studying the early stages of cancer can provide important insight into the molecular basis of the disease. We identified a preneoplastic stage in the patched (ptc) mutant mouse, a model for the brain tumor medulloblastoma. Preneoplastic cells (PNCs) are found in most ptc mutants during early adulthood, but only 15% of...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.1759909
更新日期：2009-01-15 00:00:00
abstract：:The transformer-2 (tra-2) gene of Drosophila melanogaster plays essential roles in both sexual differentiation in the female soma and spermatogenesis in the male germ line. In the female soma, tra-2 is known to act with other genes in the sex determination regulatory cascade to control the sex-specific alternative spl...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.4.5.789
更新日期：1990-05-01 00:00:00
abstract：:The ATR (ATM and Rad3-related) kinase and its regulatory partner ATRIP (ATR-interacting protein) coordinate checkpoint responses to DNA damage and replication stress. TopBP1 functions as a general activator of ATR. However, the mechanism by which TopBP1 activates ATR is unknown. Here, we show that ATRIP contains a Top...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.1666208
更新日期：2008-06-01 00:00:00
abstract：:Cascades of zygotic gene expression pattern the anterior-posterior (AP) and dorsal-ventral (DV) axes of the early Drosophila embryo. Here, we used the global run-on sequencing assay (GRO-seq) to map the genome-wide RNA polymerase distribution during early Drosophila embryogenesis, thus providing insights into how gene...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.215459.113
更新日期：2013-05-15 00:00:00
abstract：:Previous characterization of the Saccharomyces cerevisiae Spt4, Spt5, and Spt6 proteins suggested that these proteins act as transcription factors that modify chromatin structure. In this work, we report new genetic and biochemical studies of Spt4, Spt5, and Spt6 that reveal a role for these factors in transcription e...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.12.3.357
更新日期：1998-02-01 00:00:00
abstract：:Members of the metazoan nuclear receptor superfamily regulate gene expression programs in response to binding of cognate lipophilic ligands. Evolutionary studies using bioinformatics tools have concluded that lower eukaryotes, such as fungi, lack nuclear receptor homologs. Here we review recent discoveries suggesting ...
journal_title：Genes & development
pub_type： 杂志文章,评审
doi：10.1101/gad.1743009
更新日期：2009-02-15 00:00:00
abstract：:Keratinocyte growth factor (KGF), also known as fibroblast growth factor 7 (FGF7), is synthesized by skin fibroblasts. However, its mitogenic activity is on skin keratinocytes, where it is the most potent growth factor identified thus far. To explore KGF's function in vivo, we used embryonic stem cell technology to ge...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.10.2.165
更新日期：1996-01-15 00:00:00
abstract：:Jun and Fos oncoproteins form a complex that regulates transcription from promoters containing AP-1 binding sites. The 'leucine zipper' domain of both Fos and Jun is necessary for the formation of the heterodimer, but the role of specific leucine residues is unclear. We have used site-specific mutagenesis to examine t...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.3.6.770
更新日期：1989-06-01 00:00:00
abstract：:It is postulated that the symmetric organization of plant lateral organs is based on two crossed axes, the abaxial-adaxial and the lateral axes. The PRESSED FLOWER (PRS) gene, the expression and function of which are dependent on the lateral axis, is reported in this study. In the prs mutant, growth of the lateral sep...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.931001
更新日期：2001-12-15 00:00:00
abstract：:Embryonic stem (ES) cells are pluripotent cells with the ability to self-renew indefinitely. These unique properties are controlled by genetic factors and chromatin structure. The exit from the self-renewing state is accompanied by changes in epigenetic chromatin modifications such as an induction in the silencing-ass...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.1588207
更新日期：2007-10-15 00:00:00
abstract：:Cellular signaling initiated by Hedgehog binding to Patched1 has profound importance in mammalian embryogenesis, genetic disease, and cancer. Hedgehog acts as a morphogen to specify distinctive cell fates using different concentration thresholds, but our knowledge of how the concentration gradient is interpreted into ...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.1546307
更新日期：2007-05-15 00:00:00
abstract：:The gene for an essential protein subunit of nuclear RNase P from Saccharomyces cerevisiae has been cloned. The gene for this protein, RPP1, was identified by virtue of its homology with a human scleroderma autoimmune antigen, Rpp30, which copurifies with human RNase P. Epitope-tagged Rpp1 can be found in association ...
journal_title：Genes & development
pub_type： 更正并重新发布的文章,杂志文章
doi：10.1101/gad.11.21.2926
更新日期：1997-11-01 00:00:00
abstract：:In yeast, galactose triggers a rapid GAL4-dependent induction of galactose/melibiose regulon (GAL/MEL) gene transcription, and glucose represses this activation. We discovered that alterations in the physical state of the GAL4 protein correlate with activation and repression of the GAL/MEL genes. Using Western immunob...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.3.8.1157
更新日期：1989-08-01 00:00:00
abstract：:Atypical teratoid rhabdoid tumors (ATRTs) are challenging pediatric brain cancers that are predominantly associated with inactivation of the gene SMARCB1, a conserved subunit of the chromatin remodeling BAF complex, which has known contributions to developmental processes. To identify potential interactions between SM...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.339978.120
更新日期：2020-10-01 00:00:00
abstract：:The product of the zygotically active decapentaplegic (dpp) gene appears to function as a morphogen that specifies positional information in the dorsal half of the Drosophila embryo. The dorsal-specific transcription of dpp is the key step in establishing a morphogen gradient. We demonstrate here that multiple regions...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.7.4.694
更新日期：1993-04-01 00:00:00
abstract：:The p53 tumor suppressor protein is the most well studied as a regulator of transcription in the nucleus, where it exists primarily as a tetramer. However, there are other oligomeric states of p53 that are relevant to its regulation and activities. In unstressed cells, p53 is normally held in check by MDM2 that target...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.304071.117
更新日期：2018-03-01 00:00:00
abstract：:The mechanisms that determine the relative positions of floral organs, and thereby their numbers, is a poorly understood aspect of flower development. We isolated a petunia mutant, floozy (fzy), in which the formation of floral organ primordia in the outermost three floral whorls and one of the two bracts at the base ...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.219502
更新日期：2002-03-15 00:00:00
abstract：:In Xenopus embryos, a dorsal-ventral patterning gradient is generated by diffusing Chordin/bone morphogenetic protein (BMP) complexes cleaved by BMP1/Tolloid metalloproteinases in the ventral side. We developed a new BMP1/Tolloid assay using a fluorogenic Chordin peptide substrate and identified an unexpected negative...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.1839309
更新日期：2009-11-01 00:00:00
