CDK12 phosphorylates 4E-BP1 to enable mTORC1-dependent translation and mitotic genome stability.

abstract：:Ribonucleotide reductase activity is essential for progression through the cell cycle, catalyzing the rate-limiting step for the production of deoxyribonucleotides needed for DNA synthesis. The enzymatic activity of the enzyme fluctuates in the cell cycle with an activity maximum in S phase. We have identified and cha...

journal_title：Genes & development

authors： Elledge SJ,Davis RW

更新日期：1990-05-01 00:00:00

abstract：:The association of transcription corepressors SMRT and N-CoR with retinoid and thyroid receptors results in suppression of basal transcriptional activity. A key event in nuclear receptor signaling is the hormone-dependent release of corepressor and the recruitment of coactivator. Biochemical and structural studies hav...

journal_title：Genes & development

authors： Nagy L,Kao HY,Love JD,Li C,Banayo E,Gooch JT,Krishna V,Chatterjee K,Evans RM,Schwabe JW

更新日期：1999-12-15 00:00:00

abstract：:Previous studies have shown that differentiation of 3T3-L1 preadipocytes leads to the transcriptional activation of a group of adipose-specific genes. As an approach to defining the mechanism responsible for activating the expression of these genes, we investigated the binding of nuclear factors to the promoters of tw...

journal_title：Genes & development

authors： Christy RJ,Yang VW,Ntambi JM,Geiman DE,Landschulz WH,Friedman AD,Nakabeppu Y,Kelly TJ,Lane MD

更新日期：1989-09-01 00:00:00

abstract：:Pancreatic ductal adenocarcinoma (PDAC) is the fourth leading cause of cancer death in the United States with a median survival of <6 mo and a dismal 5-yr survival rate of 3%-5%. The cancer's lethal nature stems from its propensity to rapidly disseminate to the lymphatic system and distant organs. This aggressive biol...

journal_title：Genes & development

authors： Hezel AF,Kimmelman AC,Stanger BZ,Bardeesy N,Depinho RA

更新日期：2006-05-15 00:00:00

abstract：:Intracellular heme levels must be tightly regulated to maintain proper mitochondrial respiration while minimizing toxicity, but the homeostatic mechanisms are not well understood. Here we report a novel negative feedback mechanism whereby the nuclear heme receptor Rev-erbalpha tightly controls the level of its own lig...

journal_title：Genes & development

authors： Wu N,Yin L,Hanniman EA,Joshi S,Lazar MA

更新日期：2009-09-15 00:00:00

abstract：:In this issue of Genes & Development, Wierzbicki and colleagues (pp. 1825-1836) examine the current model of RNA-directed DNA methylation (RdDM) by determining genome-wide distributions of RNA polymerase V (Pol V) occupancy, siRNAs, and DNA methylation. Their data support the key role of base-pairing between Pol V tra...

journal_title：Genes & development

authors： Zhang H,Zhu JK

更新日期：2012-08-15 00:00:00

abstract：:During embryonic development, the two Caenorhabditis elegans HSN motor neurons migrate from their birthplace in the tail to positions near the middle of the embryo. Here, we demonstrate that of all cells that undergo long-range migrations, only the HSNs are affected in animals that lack function of the egl-43 gene. We...

journal_title：Genes & development

authors： Garriga G,Guenther C,Horvitz HR

更新日期：1993-11-01 00:00:00

abstract：:The ascidian tadpole represents the most simplified chordate body plan. It contains a notochord composed of just 40 cells, but as in vertebrates Brachyury is essential for notochord differentiation. Here, we show that the misexpression of the Brachyury gene (Ci-Bra) of Ciona intestinalis is sufficient to transform end...

journal_title：Genes & development

authors： Takahashi H,Hotta K,Erives A,Di Gregorio A,Zeller RW,Levine M,Satoh N

更新日期：1999-06-15 00:00:00

abstract：:Programmed cell death occurs in a highly reproducible manner during Caenorhabditis elegans development. We demonstrate that, during embryogenesis, miR-35 and miR-58 bantam family microRNAs (miRNAs) cooperate to prevent the precocious death of mothers of cells programmed to die by repressing the gene egl-1, which encod...

journal_title：Genes & development

authors： Sherrard R,Luehr S,Holzkamp H,McJunkin K,Memar N,Conradt B

更新日期：2017-01-15 00:00:00

abstract：:The gene encoding yeast U6 snRNA that is transcribed by RNA polymerase III (Pol III) contains both a TATA box upstream of the transcription start site and a downstream binding site for the factor TFIIIC. This juxtaposition of elements typical of both Pol II- and Pol III-transcribed genes raises the question of how pol...

journal_title：Genes & development

authors： Roberts S,Colbert T,Hahn S

更新日期：1995-04-01 00:00:00

abstract：:We have studied the sequence requirements for 3'-end formation of rDNA transcripts in a cell-free system and show that the generation of correct ends of mouse pre-rRNA is brought about by a two-step process that involves a bona fide termination reaction, followed by a specific trimming of the primary transcript by 10 ...

journal_title：Genes & development

authors： Kuhn A,Grummt I

更新日期：1989-02-01 00:00:00

abstract：:Appreciable advances into the process of transcript elongation by RNA polymerase II (RNAP II) have identified this stage as a dynamic and highly regulated step of the transcription cycle. Here, we discuss the many factors that regulate the elongation stage of transcription. Our discussion includes the classical elonga...

journal_title：Genes & development

authors： Sims RJ 3rd,Belotserkovskaya R,Reinberg D

更新日期：2004-10-15 00:00:00

abstract：:Chemical mutagenesis in the mouse is a powerful approach for phenotype-driven genetics, but questions remain about the efficiency with which new mutations ascertained by their phenotype can be localized and identified, and that knowledge applied to a specific biological problem. During a global screen for dominant phe...

journal_title：Genes & development

authors： Fitch KR,McGowan KA,van Raamsdonk CD,Fuchs H,Lee D,Puech A,Hérault Y,Threadgill DW,Hrabé de Angelis M,Barsh GS

更新日期：2003-01-15 00:00:00

abstract：:Mitotic duration is determined by activation of the anaphase-promoting complex/cyclosome (APC/C) bound to its coactivator, Cdc20. Kinetochores, the microtubule-interacting machines on chromosomes, restrain mitotic exit when not attached to spindle microtubules by generating a Cdc20-containing complex that inhibits the...

journal_title：Genes & development

authors： Kim T,Lara-Gonzalez P,Prevo B,Meitinger F,Cheerambathur DK,Oegema K,Desai A

更新日期：2017-06-01 00:00:00

abstract：:The Wnt signaling cascade is a central regulator of cell fate determination during embryonic development, whose deregulation contributes to oncogenesis. Naked cuticle is the first Wnt-induced antagonist found in this pathway, establishing a negative-feedback loop that limits the Wnt signal required for early segmentat...

journal_title：Genes & development

authors： Creyghton MP,Roël G,Eichhorn PJ,Hijmans EM,Maurer I,Destrée O,Bernards R

更新日期：2005-02-01 00:00:00

abstract：:The retinoblastoma tumor suppressor (pRb) protein associates with chromatin and regulates gene expression. Numerous studies have identified Rb-dependent RNA signatures, but the proteomic effects of Rb loss are largely unexplored. We acutely ablated Rb in adult mice and conducted a quantitative analysis of RNA and prot...

journal_title：Genes & development

authors： Nicolay BN,Danielian PS,Kottakis F,Lapek JD Jr,Sanidas I,Miles WO,Dehnad M,Tschöp K,Gierut JJ,Manning AL,Morris R,Haigis K,Bardeesy N,Lees JA,Haas W,Dyson NJ

更新日期：2015-09-01 00:00:00

abstract：:Hepatitis B virus (HBV) infects humans and causes a wide range of clinical manifestations, from acute hepatitis to hepatocellular carcinoma (HCC). The HBV genome contains multiple promoters with gene expression regulated predominantly by the cellular transcription initiation machinery. Accordingly, the HBV-encoded pX,...

journal_title：Genes & development

authors： Haviv I,Matza Y,Shaul Y

更新日期：1998-04-15 00:00:00

abstract：:The p53 tumor suppressor is often disrupted in human cancers by the acquisition of missense mutations. We generated mice with a missense mutation at codon 172 that mimics the p53R175H hot spot mutation in human cancer. p53 homozygous mutant mice have unstable mutant p53 in normal cells and stabilize mutant p53 in some...

journal_title：Genes & development

authors： Terzian T,Suh YA,Iwakuma T,Post SM,Neumann M,Lang GA,Van Pelt CS,Lozano G

更新日期：2008-05-15 00:00:00

abstract：:A long-standing model postulates that X-chromosome dosage compensation in Drosophila occurs by twofold up-regulation of the single male X, but previous data cannot exclude an alternative model, in which male autosomes are down-regulated to balance gene expression. To distinguish between the two models, we used RNA int...

journal_title：Genes & development

authors： Hamada FN,Park PJ,Gordadze PR,Kuroda MI

更新日期：2005-10-01 00:00:00

abstract：:We describe evidence that a regulatory B subunit of protein phosphatase 2A (PP2A) positively regulates an RTK-Ras-MAP kinase signaling cascade during Caenorhabditis elegans vulval induction. Although reduction of sur-6 PP2A-B function causes few vulval induction defects in an otherwise wild-type background, sur-6 PP2A...

journal_title：Genes & development

authors： Sieburth DS,Sundaram M,Howard RM,Han M

更新日期：1999-10-01 00:00:00

abstract：:The fission yeast telomerase RNA (TER1) precursor harbors an intron immediately downstream from its mature 3' end. Unlike most introns, which are removed from precursor RNAs by the spliceosome in two sequential but tightly coupled transesterification reactions, TER1 only undergoes the first cleavage reaction during te...

journal_title：Genes & development

authors： Kannan R,Hartnett S,Voelker RB,Berglund JA,Staley JP,Baumann P

更新日期：2013-03-15 00:00:00

abstract：:Pax3 is a key transcription factor implicated in development and human disease. To dissect the role of Pax3 in myogenesis and establish whether it is a repressor or activator, we generated loss- and gain-of-function alleles by targeting an nLacZ reporter and a sequence encoding the oncogenic fusion protein PAX3-FKHR i...

journal_title：Genes & development

authors： Relaix F,Polimeni M,Rocancourt D,Ponzetto C,Schäfer BW,Buckingham M

更新日期：2003-12-01 00:00:00

abstract：:Cellular signaling initiated by Hedgehog binding to Patched1 has profound importance in mammalian embryogenesis, genetic disease, and cancer. Hedgehog acts as a morphogen to specify distinctive cell fates using different concentration thresholds, but our knowledge of how the concentration gradient is interpreted into ...

journal_title：Genes & development

authors： Martinelli DC,Fan CM

更新日期：2007-05-15 00:00:00

abstract：:How dividing mammalian cells overcome blocks to DNA replication by DNA damage, depleted nucleotide pools, or template-bound proteins is unclear. Here, we show that the response to blocked replication requires BRCA2, a suppressor of human breast cancer. By using two-dimensional gel electrophoresis, we demonstrate that ...

journal_title：Genes & development

authors： Lomonosov M,Anand S,Sangrithi M,Davies R,Venkitaraman AR

更新日期：2003-12-15 00:00:00

abstract：:Class Ia phosphatidylinositol 3 kinase (PI3K) is required for oncogenic receptor-mediated transformation; however, the individual roles of the two commonly expressed class Ia PI3K isoforms in oncogenic receptor signaling have not been elucidated in vivo. Here, we show that genetic ablation of p110α blocks tumor format...

journal_title：Genes & development

authors： Utermark T,Rao T,Cheng H,Wang Q,Lee SH,Wang ZC,Iglehart JD,Roberts TM,Muller WJ,Zhao JJ

更新日期：2012-07-15 00:00:00

abstract：:The tobacco mitogen-activated protein kinase (MAPK) cascade, which includes MAPK NRK1/NTF6, positively regulates expansion of the cytokinetic machinery known as the phragmoplast, which is followed by the synthesis of cell plates for completion of cell division. However, molecular events lying between the MAPK and phra...

journal_title：Genes & development

authors： Sasabe M,Soyano T,Takahashi Y,Sonobe S,Igarashi H,Itoh TJ,Hidaka M,Machida Y

更新日期：2006-04-15 00:00:00

abstract：:During development of the somatic macronucleus of Tetrahymena thermophila, the rDNA is excised from its germ-line chromosome, rearranged into a palindrome, and amplified to 10(4) copies. We have identified a cis-acting germ-line mutation, rmm11/6, that prevents amplification of the rDNA in all but approximately 1 in 1...

journal_title：Genes & development

authors： Kapler GM,Blackburn EH

更新日期：1994-01-01 00:00:00

abstract：:Chromosomal translocations are a hallmark of hematopoietic malignancies. The initial molecular events or pathways that lead to translocations in hematopoietic cells are largely unknown, particularly in the stem cell-enriched population postulated to be the initial target for these events. We used in vitro differentiat...

journal_title：Genes & development

authors： Francis R,Richardson C

更新日期：2007-05-01 00:00:00

abstract：:The Notch signaling pathway is essential for embryonic vascular development in vertebrates. Here we show that mouse embryos heterozygous for a targeted mutation in the gene encoding the DLL4 ligand exhibit haploinsufficient lethality because of defects in vascular remodeling. We also describe vascular defects in embry...

journal_title：Genes & development

authors： Krebs LT,Shutter JR,Tanigaki K,Honjo T,Stark KL,Gridley T

更新日期：2004-10-15 00:00:00

abstract：:Morphogenesis of the insect kidney, the Malpighian tubules, is controlled in Drosophila by a single large cell, the tip cell. It has been postulated that this cell sends out a mitogenic signal that induces the division of neighboring cells. The signal and the molecules that receive it have remained elusive. We show th...

journal_title：Genes & development

authors： Kerber B,Fellert S,Hoch M

更新日期：1998-06-15 00:00:00