Abstract：
:Interruption of translation in Escherichia coli can lead to the addition of an 11-residue carboxy-terminal peptide tail to the nascent chain. This modification is mediated by SsrA RNA (also called 10Sa RNA and tmRNA) and marks the tagged polypeptide for proteolysis. Degradation in vivo of lambda repressor amino-terminal domain variants bearing this carboxy-terminal SsrA peptide tag is shown here to depend on the cytoplasmic proteases ClpXP and ClpAP. Degradation in vitro of SsrA-tagged substrates was reproduced with purified components and required a substrate with a wild-type SsrA tail, the presence of both ClpP and either ClpA or ClpX, and ATP. Clp-dependent proteolysis accounts for most degradation of SsrA-tagged amino-domain substrates at 32 degrees C, but additional proteases contribute to the degradation of some of these SsrA-tagged substrates at 39 degrees C. The existence of multiple cytoplasmic proteases that function in SsrA quality-control surveillance suggests that the SsrA tag is designed to serve as a relatively promiscuous signal for proteolysis. Having diverse degradation systems able to recognize this tag may increase degradation capacity, permit degradation of a wide variety of different tagged proteins, or allow SsrA-tagged proteins to be degraded under different growth conditions.
journal_name
Genes Devjournal_title
Genes & developmentauthors
Gottesman S,Roche E,Zhou Y,Sauer RTdoi
10.1101/gad.12.9.1338subject
Has Abstractpub_date
1998-05-01 00:00:00pages
1338-47issue
9eissn
0890-9369issn
1549-5477journal_volume
12pub_type
杂志文章abstract：:We investigated the regulation of the activin/nodal-inducible distal element (DE) of the Xenopus goosecoid (gsc) promoter. On the basis of its interaction with the DE, we isolated a Xenopus homolog of the human Williams-Beuren syndrome critical region 11 (XWBSCR11), and further, show that it interacts with pathway-spe...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.963802
更新日期：2002-04-01 00:00:00
abstract：:FISH analysis of well-spread chromosomes reveals that homologs are paired in vegetatively growing budding yeast diploid cells, via multiple interstitial interactions, and independent of recA homologs and mating type heterozygosity. Pairing is present during G1 and G2, and in cells arrested at G1 by mating pheromone, b...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.13.12.1627
更新日期：1999-06-15 00:00:00
abstract：:While microRNAs (miRNAs) regulate the vast majority of protein-encoding transcripts, little is known about how miRNAs themselves are degraded. We recently described Tudor-staphylococcal/micrococcal-like nuclease (TSN)-mediated miRNA decay (TumiD) as a cellular pathway in which the nuclease TSN promotes the decay of mi...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.303537.117
更新日期：2017-07-15 00:00:00
abstract：:Infection of metazoan cells with some viruses alters the balance of cellular mRNA export to favor viral RNA export and to retain cellular transcripts in the nucleus. Here, evidence is presented to show that the herpes simplex virus 1 (HSV-1) essential regulatory protein ICP27, which inhibits host cell-splicing, result...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.12.6.868
更新日期：1998-03-15 00:00:00
abstract：:Translation of encephalomyocarditis virus (EMCV) mRNA occurs by ribosomal internal entry into the 5'-nontranslated region (5' NTR) rather than by ribosomal scanning. The internal ribosomal entry site (IRES) in the EMCV 5' NTR was determined by in vitro translation with RNAs that were generated by in vitro transcriptio...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.4.9.1560
更新日期：1990-09-01 00:00:00
abstract：:The yeast SAGA (Spt-Ada-Gcn5-acetyltransferase) coactivator complex exerts functions in gene expression, including activator interaction, histone acetylation, histone deubiquitination, mRNA export, chromatin recognition, and regulation of the basal transcription machinery. These diverse functions involve distinct modu...
journal_title：Genes & development
pub_type： 杂志文章,评审
doi：10.1101/gad.184705.111
更新日期：2012-03-15 00:00:00
abstract：:The transcription factor NF-kappaB is a critical regulator of inflammatory and cell survival signals. Proteasomal degradation of NF-kappaB subunits plays an important role in the termination of NF-kappaB activity, and at least one of the identified ubiquitin ligases is a multimeric complex containing Copper Metabolism...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.1748409
更新日期：2009-04-01 00:00:00
abstract：:We characterized a gene, extradenticle, which seems to interact with a specific subset of Drosophila homeo domain proteins, possibly affecting their target specificity. This interpretation is based on an examination of the zygotic and maternal effect phenotypes of extradenticle mutations. In embryos with reduced level...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.4.7.1209
更新日期：1990-07-01 00:00:00
abstract：:P-element transformants of a single rRNA gene (rDNA) were used to investigate the relationship between the organization of the nucleolus organizer (NO) and rDNA function in Drosophila melanogaster. In situ hybridization to rRNA in polytene nuclei of salivary glands demonstrated that an rRNA gene can be transcribed at ...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.2.12b.1745
更新日期：1988-12-01 00:00:00
abstract：:The homeless (hls) gene of Drosophila is required for anteroposterior and dorsoventral axis formation during oogenesis. At a low frequency, females homozygous for mutations in hls generate early egg chambers in which the oocyte is positioned incorrectly within the cyst. At a high frequency, late-stage egg chambers exh...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.9.20.2495
更新日期：1995-10-15 00:00:00
abstract：:We describe evidence that a regulatory B subunit of protein phosphatase 2A (PP2A) positively regulates an RTK-Ras-MAP kinase signaling cascade during Caenorhabditis elegans vulval induction. Although reduction of sur-6 PP2A-B function causes few vulval induction defects in an otherwise wild-type background, sur-6 PP2A...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.13.19.2562
更新日期：1999-10-01 00:00:00
abstract：:In eubacteria, the ClpS adaptor has been proposed to be essential for degradation of N-end rule substrates by the AAA(+) protease ClpAP. To test this model, we assayed degradation of substrates bearing N-end rule sequences isolated in a genetic screen for efficient degradation tags. ClpS was not vital for degradation ...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.1511907
更新日期：2007-02-15 00:00:00
abstract：:AU-rich elements (AREs, usually containing repeated copies of AUUUA), when present in the 3'-untranslated regions (UTRs) of many mammalian mRNAs, confer instability on their host RNA molecules. The viral small nuclear RNA (snRNA) Herpesvirus saimiri U RNA 1 (HSUR 1) also contains an AUUUA-rich sequence. Here, we repor...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.11.19.2557
更新日期：1997-10-01 00:00:00
abstract：:Caenorhabditis elegans GLD-1, a KH motif containing RNA-binding protein of the GSG/STAR subfamily, controls diverse aspects of germ line development, suggesting that it may have multiple mRNA targets. We used an immunoprecipitation/subtractive hybridization/cloning strategy to identify 15 mRNAs that are putative targe...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.915901
更新日期：2001-09-15 00:00:00
abstract：:The mouse Nedd5 gene encodes a 41.5-kD GTPase similar to the Saccharomyces and Drosophila septins essential for cytokinesis. Nedd5 accumulates near the contractile ring from anaphase through telophase, and finally condenses into the midbody. Microinjection of anti-Nedd5 antibody interferes with cytokinesis, giving ris...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.11.12.1535
更新日期：1997-06-15 00:00:00
abstract：:Long understood as a bona fide tumor suppressor that safeguards the integrity of the genome via regulating numerous cellular outcomes, p53 may also exert its decisive and versatile functions by controlling DNA methylation. In this issue of Genes & Development, Tovy and colleagues (pp. 959-972) report that, in naïve mo...
journal_title：Genes & development
pub_type： 评论,杂志文章,评审
doi：10.1101/gad.302364.117
更新日期：2017-05-15 00:00:00
abstract：:We describe three strains of transgenic mice derived by embryo microinjection of DNA consisting of a long terminal repeat (LTR) of Moloney murine sarcoma virus (Mo-MSV) linked to the murine c-mos coding sequences. Southern analysis of the genomic DNA of these strains suggested that in each case the transgene had integ...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.1.10.1327
更新日期：1987-12-01 00:00:00
abstract：:Early endoderm formation in zebrafish requires at least three loci that function downstream of Nodal signaling but upstream of the early endodermal marker sox17: bonnie and clyde (bon), faust (fau), and casanova (cas). cas mutants show the most severe phenotype as they do not form any gut tissue and lack all sox17 exp...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.892301
更新日期：2001-06-15 00:00:00
abstract：:Cap-dependent translation is mediated by eIF4F, a protein complex composed of three subunits as follows: eIF4E, which recognizes the mRNA 5' cap structure; eIF4A, an RNA-helicase; and eIF4G, a scaffolding protein that binds eIF4E, eIF4A, and the eIF4E-kinase Mnk1 simultaneously. eIF4E is hypophosphorylated and cap-dep...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.889201
更新日期：2001-08-15 00:00:00
abstract：:Early during Dictyostelium development a fundamental cell-fate decision establishes the anteroposterior (prestalk/prespore) axis. Signaling via the 7-transmembrane cAMP receptor CAR4 is essential for creating and maintaining a normal pattern; car4-null alleles have decreased levels of prestalk-specific mRNAs but enhan...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.11.16.2112
更新日期：1997-08-15 00:00:00
abstract：:A new gene, SCH9, was isolated from Saccharomyces cerevisiae by its ability to complement a cdc25ts mutation. Sequence analysis indicates that it encodes a 90,000-dalton protein with a carboxy-terminal domain homologous to yeast and mammalian cAMP-dependent protein kinase catalytic subunits. In addition to suppressing...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.2.5.517
更新日期：1988-05-01 00:00:00
abstract：:The HAT4 gene from the plant Arabidopsis thaliana encodes a homeo domain protein that contains a leucine zipper motif. Homeo domain-leucine zipper (HD-Zip) proteins have not been found in animal systems, suggesting that HAT4 may define a new family of transcription factors that regulate higher plant development. To ex...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.7.3.367
更新日期：1993-03-01 00:00:00
abstract：:Macrophage activation by the proinflammatory cytokine interferon-gamma (IFN-gamma) is a critical component of the host innate response to bacterial pathogenesis. However, the precise nature of the IFN-gamma-induced activation pathway is not known. Here we show using genome-wide expression and chromatin-binding profili...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.1553007
更新日期：2007-08-01 00:00:00
abstract：:Amplification of 8p11-12 in human breast cancers is associated with increased proliferation and tumor grade and reduced metastasis-free patient survival. We identified Zeppo1 (zinc finger elbow-related proline domain protein 1) (FLJ14299/ZNF703) within this amplicon as a regulator of cell adhesion, migration, and prol...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.1998111
更新日期：2011-03-01 00:00:00
abstract：:Many small nucleolar RNAs (snoRNAs) in vertebrates are encoded within introns of protein genes. We have reported previously that two isoforms of human U17 snoRNA are encoded in introns of the cell-cycle regulatory gene, RCC1. We have now investigated the mechanism of processing of U17 RNAs and of another intron-encode...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.9.11.1411
更新日期：1995-06-01 00:00:00
abstract：:The human RNA polymerase II (RNAPII)-associated factor complex (hPAFc) and its individual subunits have been implicated in human diseases, including cancer. However, its involvement in breast cancer awaits investigation. Using data mining and human breast cancer tissue microarrays, we found that Ctr9, the key scaffold...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.268722.115
更新日期：2015-10-15 00:00:00
abstract：:The human T cell-specific transcription factor TCF-1 alpha plays a key role in the tissue-specific activation of the T cell receptor (TCR) C alpha enhancer and binds to pyrimidine-rich elements (5'-PyCTTTG-3') present in a variety of other T cell-specific control regions. Using amino acid sequence information derived ...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.5.4.656
更新日期：1991-04-01 00:00:00
abstract：:A prominent feature of cell differentiation is the initiation and maintenance of an irreversible cell cycle arrest with the complex involvement of the retinoblastoma (RB) family (RB, p130, p107). We have isolated the HBP1 transcriptional repressor as a potential target of the RB family in differentiated cells. By homo...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.11.3.383
更新日期：1997-02-01 00:00:00
abstract：:Male sexual development in the nematode Caenorhabditis elegans requires the genes fem-1, fem-2, and fem-3. The current model of sex determination portrays the FEM proteins as components of a novel signal transduction pathway, but the mechanisms involved in signaling through the pathway are not understood. We report th...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.10.18.2314
更新日期：1996-09-15 00:00:00
abstract：:Thousands of eukaryotic protein-coding genes are noncanonically spliced to produce circular RNAs. Bioinformatics has indicated that long introns generally flank exons that circularize in Drosophila, but the underlying mechanisms by which these circular RNAs are generated are largely unknown. Here, using extensive muta...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.270421.115
更新日期：2015-10-15 00:00:00
