当前位置： SCI文献检索 > GENES & DEVELOPMENT期刊下所有文献 > ICP27 mediates HSV RNA export by shuttling through a leucine-rich nuclear export signal and binding viral intronless RNAs through an RGG motif.

ICP27 mediates HSV RNA export by shuttling through a leucine-rich nuclear export signal and binding viral intronless RNAs through an RGG motif.

Abstract：

:Infection of metazoan cells with some viruses alters the balance of cellular mRNA export to favor viral RNA export and to retain cellular transcripts in the nucleus. Here, evidence is presented to show that the herpes simplex virus 1 (HSV-1) essential regulatory protein ICP27, which inhibits host cell-splicing, resulting in the accumulation of unspliced transcripts in the nucleus, mediates RNA export of viral intronless mRNAs. ICP27 was shown to shuttle between the nucleus and cytoplasm through a leucine-rich nuclear export signal, which alone was able to direct the export of the heterologous green fluorescent protein. In vivo UV irradiation studies demonstrated that ICP27 could be crosslinked to poly(A)+ RNA in the nucleus and the cytoplasm, supporting a role in export. Furthermore, the amount of hnRNP A1, which has been implicated in the export of cellular spliced mRNAs, that was bound to poly(A)+ RNA in HSV-1-infected cells was reduced compared with uninfected cells. In addition, it was demonstrated that ICP27 bound seven intronless HSV-1 transcripts in both the nucleus and the cytoplasm, and export of these transcripts was diminished substantially during infection with an ICP27 null mutant virus. In contrast, ICP27 did not bind to two HSV-1 mRNAs that undergo splicing. Finally, binding of ICP27 to RNA in vivo required an arginine-glycine region that resembles an RGG box. These results indicate that ICP27 is an important viral export factor that promotes the transport of HSV-1 intronless RNAs.

journal_name

Genes Dev

journal_title

Genes & development

authors

Sandri-Goldin RM

doi

10.1101/gad.12.6.868

subject

Has Abstract

pub_date

1998-03-15 00:00:00

pages

868-79

issue

6

eissn

0890-9369

issn

1549-5477

journal_volume

12

pub_type

杂志文章

相关文献

GENES & DEVELOPMENT文献大全
  • Developmentally regulated fetal thymic and extrathymic T-cell receptor gamma delta gene expression.

    abstract：:The gamma delta T-cell receptor (TCR) is the first TCR to be expressed in ontogeny in all vertebrates in which it has been examined thoroughly. Murine gamma delta cell-surface protein is detected by the fourteenth day of gestation. In this work, the activation of gamma delta RNA has been studied. Data indicate that th...

    journal_title：Genes & development

    pub_type： 杂志文章

    doi：10.1101/gad.4.8.1304

    authors： Carding SR,Kyes S,Jenkinson EJ,Kingston R,Bottomly K,Owen JJ,Hayday AC

    更新日期：1990-08-01 00:00:00

  • AMP-activated protein kinase: an energy sensor that regulates all aspects of cell function.

    abstract：:AMP-activated protein kinase (AMPK) is a sensor of energy status that maintains cellular energy homeostasis. It arose very early during eukaryotic evolution, and its ancestral role may have been in the response to starvation. Recent work shows that the kinase is activated by increases not only in AMP, but also in ADP....

    journal_title：Genes & development

    pub_type： 杂志文章,评审

    doi：10.1101/gad.17420111

    authors： Hardie DG

    更新日期：2011-09-15 00:00:00

  • Mouse TU tagging: a chemical/genetic intersectional method for purifying cell type-specific nascent RNA.

    abstract：:Transcriptional profiling is a powerful approach for understanding development and disease. Current cell type-specific RNA purification methods have limitations, including cell dissociation trauma or inability to identify all RNA species. Here, we describe "mouse thiouracil (TU) tagging," a genetic and chemical inters...

    journal_title：Genes & development

    pub_type： 杂志文章

    doi：10.1101/gad.205278.112

    authors： Gay L,Miller MR,Ventura PB,Devasthali V,Vue Z,Thompson HL,Temple S,Zong H,Cleary MD,Stankunas K,Doe CQ

    更新日期：2013-01-01 00:00:00

  • Induction of cell fate in the Drosophila retina: the bride of sevenless protein is predicted to contain a large extracellular domain and seven transmembrane segments.

    abstract：:Previous genetic mosaic studies established that expression of the Drosophila bride of sevenless (boss) gene is required in photoreceptor neuron R8 for the development of photoreceptor neuron R7. This led to the proposal that boss encodes or regulates an R7-specific inductive cue. We have identified the boss gene base...

    journal_title：Genes & development

    pub_type： 杂志文章

    doi：10.1101/gad.4.11.1835

    authors： Hart AC,Krämer H,Van Vactor DL Jr,Paidhungat M,Zipursky SL

    更新日期：1990-11-01 00:00:00

  • The transcriptional activator PAX3-FKHR rescues the defects of Pax3 mutant mice but induces a myogenic gain-of-function phenotype with ligand-independent activation of Met signaling in vivo.

    abstract：:Pax3 is a key transcription factor implicated in development and human disease. To dissect the role of Pax3 in myogenesis and establish whether it is a repressor or activator, we generated loss- and gain-of-function alleles by targeting an nLacZ reporter and a sequence encoding the oncogenic fusion protein PAX3-FKHR i...

    journal_title：Genes & development

    pub_type： 杂志文章

    doi：10.1101/gad.281203

    authors： Relaix F,Polimeni M,Rocancourt D,Ponzetto C,Schäfer BW,Buckingham M

    更新日期：2003-12-01 00:00:00

  • Defects in Saccharomyces cerevisiae protein phosphatase type I activate the spindle/kinetochore checkpoint.

    abstract：:A conditional allele of type 1 protein phosphatase (glc7-129) in Saccharomyces cerevisiae causes first cycle arrest in G2/M, characterized by cells with a short spindle and high H1 kinase activity. Point-of-execution experiments indicate Glc7p function is required in G2/M just before anaphase for the completion of mit...

    journal_title：Genes & development

    pub_type： 杂志文章

    doi：10.1101/gad.13.5.517

    authors： Bloecher A,Tatchell K

    更新日期：1999-03-01 00:00:00

  • Purkinje cell protein-2 regulatory regions and transgene expression in cerebellar compartments.

    abstract：:The Purkinje cell protein 2 (Pcp-2) is expressed in cerebellar Purkinje cells and retinal bipolar neurons. To illuminate how Pcp-2 expression is restricted to only two neuronal types and to derive tools to express heterologous genes in these neuronal subpopulations, genomic sequences of the mouse Pcp-2 gene have been ...

    journal_title：Genes & development

    pub_type： 杂志文章

    doi：10.1101/gad.5.7.1136

    authors： Vandaele S,Nordquist DT,Feddersen RM,Tretjakoff I,Peterson AC,Orr HT

    更新日期：1991-07-01 00:00:00

  • A tetramer of the Flp recombinase silences the trimers within it during resolution of a Holliday junction substrate.

    abstract：:Recombination catalyzed by the Flp site-specific recombinase involves breakage and joining of four DNA strands between two target substrates. The reaction is carried out in two steps of pairwise strand exchanges by a DNA-protein assembly in which four Flp monomers act cooperatively to execute strand cleavage and joini...

    journal_title：Genes & development

    pub_type： 杂志文章

    doi：10.1101/gad.11.18.2438

    authors： Lee J,Jayaram M

    更新日期：1997-09-15 00:00:00

  • Novel pro- and anti-recombination activities of the Bloom's syndrome helicase.

    abstract：:Bloom's syndrome (BS) is an autosomal recessive disorder characterized by a strong cancer predisposition. The defining feature of BS is extreme genome instability. The gene mutated in Bloom's syndrome, BLM, encodes a DNA helicase (BLM) of the RecQ family. BLM plays a role in homologous recombination; however, its exac...

    journal_title：Genes & development

    pub_type： 杂志文章

    doi：10.1101/gad.1609007

    authors： Bugreev DV,Yu X,Egelman EH,Mazin AV

    更新日期：2007-12-01 00:00:00

  • A conserved ncRNA-binding protein recruits silencing factors to heterochromatin through an RNAi-independent mechanism.

    abstract：:Long noncoding RNAs (lncRNAs) can trigger repressive chromatin, but how they recruit silencing factors remains unclear. In Schizosaccharomyces pombe, heterochromatin assembly on transcribed noncoding pericentromeric repeats requires both RNAi and RNAi-independent mechanisms. In Saccharomyces cerevisiae, which lacks a ...

    journal_title：Genes & development

    pub_type： 杂志文章

    doi：10.1101/gad.226019.113

    authors： Marina DB,Shankar S,Natarajan P,Finn KJ,Madhani HD

    更新日期：2013-09-01 00:00:00

  • Cellular projections from sensory hair cells form polarity-specific scaffolds during synaptogenesis.

    abstract：:The assembly of a nervous system requires the extension of axons and dendrites to specific regions where they are matched with appropriate synaptic targets. Although the cues that guide long-range outgrowth have been characterized extensively, additional mechanisms are required to explain short-range guidance in neura...

    journal_title：Genes & development

    pub_type： 杂志文章

    doi：10.1101/gad.259838.115

    authors： Dow E,Siletti K,Hudspeth AJ

    更新日期：2015-05-15 00:00:00

  • Modulating the outer membrane with small RNAs.

    abstract：:MicF, one of the first chromosomally encoded regulatory small RNAs (sRNAs) to be discovered, was found to modulate the expression of OmpF, an abundant outer membrane protein. Several recent papers have now shown that this is not an isolated case. At least five other sRNAs also regulate the synthesis of outer membrane ...

    journal_title：Genes & development

    pub_type： 杂志文章,评审

    doi：10.1101/gad.1457506

    authors： Guillier M,Gottesman S,Storz G

    更新日期：2006-09-01 00:00:00

  • Induction of medulloblastomas in p53-null mutant mice by somatic inactivation of Rb in the external granular layer cells of the cerebellum.

    abstract：:Medulloblastomas are among the most common malignancies in childhood, and they are associated with substantial mortality and morbidity. The molecular pathogenesis as well as the ontogeny of these neoplasms is still poorly understood. We have generated a mouse model for medulloblastoma by Cre-LoxP-mediated inactivation...

    journal_title：Genes & development

    pub_type： 杂志文章

    doi：

    authors： Marino S,Vooijs M,van Der Gulden H,Jonkers J,Berns A

    更新日期：2000-04-15 00:00:00

  • The HIV-1 Tat protein activates transcription from an upstream DNA-binding site: implications for Tat function.

    abstract：:The Tat protein of human immunodeficiency virus type 1 (HIV-1) activates transcription following binding to nascent trans-activation response (TAR) RNA downstream of the transcription start site. Because Tat functions when bound to RNA, and in a position-dependent manner, it has been proposed that Tat works by a novel...

    journal_title：Genes & development

    pub_type： 杂志文章

    doi：10.1101/gad.5.12b.2496

    authors： Southgate CD,Green MR

    更新日期：1991-12-01 00:00:00

  • The embryonic lethality of homozygous lethal yellow mice (Ay/Ay) is associated with the disruption of a novel RNA-binding protein.

    abstract：:Lethal yellow (Ay) is a mutation at the mouse agouti (a) locus that is associated with an all-yellow coat color, obesity, diabetes, tumors in heterozygotes, and preimplantation embryonic lethality in homozygotes. Previously, we cloned and characterized the wild-type agouti gene and demonstrated that it expresses a 0.8...

    journal_title：Genes & development

    pub_type： 杂志文章

    doi：10.1101/gad.7.7a.1203

    authors： Michaud EJ,Bultman SJ,Stubbs LJ,Woychik RP

    更新日期：1993-07-01 00:00:00

  • Regulated phosphorylation and dephosphorylation of GAL4, a transcriptional activator.

    abstract：:In yeast, galactose triggers a rapid GAL4-dependent induction of galactose/melibiose regulon (GAL/MEL) gene transcription, and glucose represses this activation. We discovered that alterations in the physical state of the GAL4 protein correlate with activation and repression of the GAL/MEL genes. Using Western immunob...

    journal_title：Genes & development

    pub_type： 杂志文章

    doi：10.1101/gad.3.8.1157

    authors： Mylin LM,Bhat JP,Hopper JE

    更新日期：1989-08-01 00:00:00

  • Anti-diabetic rosiglitazone remodels the adipocyte transcriptome by redistributing transcription to PPARγ-driven enhancers.

    abstract：:Rosiglitazone (rosi) is a powerful insulin sensitizer, but serious toxicities have curtailed its widespread clinical use. Rosi functions as a high-affinity ligand for peroxisome proliferator-activated receptor γ (PPARγ), the adipocyte-predominant nuclear receptor (NR). The classic model, involving binding of ligand to...

    journal_title：Genes & development

    pub_type： 杂志文章

    doi：10.1101/gad.237628.114

    authors： Step SE,Lim HW,Marinis JM,Prokesch A,Steger DJ,You SH,Won KJ,Lazar MA

    更新日期：2014-05-01 00:00:00

  • A model for the abrogation of the SOS response by an SOS protein: a negatively charged helix in DinI mimics DNA in its interaction with RecA.

    abstract：:DinI is a recently described negative regulator of the SOS response in Escherichia coli. Here we show that it physically interacts with RecA and prevents the binding of single-stranded DNA to RecA, which is required for the activation of the latter. DinI also displaces ssDNA from a stable RecA-DNA cofilament, thus eli...

    journal_title：Genes & development

    pub_type： 杂志文章

    doi：10.1101/gad.862901

    authors： Voloshin ON,Ramirez BE,Bax A,Camerini-Otero RD

    更新日期：2001-02-15 00:00:00

  • EGF domain swap converts a drosophila EGF receptor activator into an inhibitor.

    abstract：:In Drosophila the function of the epidermal growth factor (EGF) receptor is modulated zygotically by three EGF-like proteins: Spitz (Spi), which is a potent activator; Vein (Vn), which is a moderate activator; and Argos (Aos), which is an inhibitor. Chimeric molecules were constructed in which the EGF domain of Vn was...

    journal_title：Genes & development

    pub_type： 杂志文章

    doi：10.1101/gad.12.7.908

    authors： Schnepp B,Donaldson T,Grumbling G,Ostrowski S,Schweitzer R,Shilo BZ,Simcox A

    更新日期：1998-04-01 00:00:00

  • DNA damage activates p53 through a phosphorylation-acetylation cascade.

    abstract：:Activation of p53-mediated transcription is a critical cellular response to DNA damage. p53 stability and site-specific DNA-binding activity and, therefore, transcriptional activity, are modulated by post-translational modifications including phosphorylation and acetylation. Here we show that p53 is acetylated in vitr...

    journal_title：Genes & development

    pub_type： 杂志文章

    doi：10.1101/gad.12.18.2831

    authors： Sakaguchi K,Herrera JE,Saito S,Miki T,Bustin M,Vassilev A,Anderson CW,Appella E

    更新日期：1998-09-15 00:00:00

  • Control of C. elegans hermaphrodite gonad size and shape by vab-3/Pax6-mediated regulation of integrin receptors.

    abstract：:Integrin receptors for extracellular matrix are critical for cell motility, but the signals that determine when to stop are not known. Analysis of distal tip cell (DTC) migration during gonadogenesis in Caenorhabditis elegans has revealed the importance of transcription factor vab-3/Pax6 in regulating the alpha integr...

    journal_title：Genes & development

    pub_type： 杂志文章

    doi：10.1101/gad.1534807

    authors： Meighan CM,Schwarzbauer JE

    更新日期：2007-07-01 00:00:00

  • Specific regulation of Xenopus chromosomal 5S rRNA gene transcription in vivo by histone H1.

    abstract：:The incorporation of histone H1 into chromatin during embryogenesis directs the specific repression of the Xenopus oocyte 5S rRNA genes. An increase in histone H1 content specifically restricts TFIIIA-activated transcription, and a decrease in histone H1 within chromatin facilitates the activation of the oocyte 5S rRN...

    journal_title：Genes & development

    pub_type： 杂志文章

    doi：10.1101/gad.8.10.1147

    authors： Bouvet P,Dimitrov S,Wolffe AP

    更新日期：1994-05-15 00:00:00

  • Brg1 promotes both tumor-suppressive and oncogenic activities at distinct stages of pancreatic cancer formation.

    abstract：:Pancreatic ductal adenocarcinoma (PDA) develops predominantly through pancreatic intraepithelial neoplasia (PanIN) and intraductal papillary mucinous neoplasm (IPMN) precursor lesions. Pancreatic acinar cells are reprogrammed to a "ductal-like" state during PanIN-PDA formation. Here, we demonstrate a parallel mechanis...

    journal_title：Genes & development

    pub_type： 杂志文章

    doi：10.1101/gad.256628.114

    authors： Roy N,Malik S,Villanueva KE,Urano A,Lu X,Von Figura G,Seeley ES,Dawson DW,Collisson EA,Hebrok M

    更新日期：2015-03-15 00:00:00

  • Genetic and biochemical definition of the Hedgehog receptor.

    abstract：:Although the transporter-like protein Patched (Ptc) is genetically implicated in reception of the extracellular Hedgehog (Hh) protein signal, a clear definition of the Hh receptor is complicated by the existence of additional Hh-binding proteins and, in Drosophila, by the lack of physical evidence for direct binding o...

    journal_title：Genes & development

    pub_type： 杂志文章

    doi：10.1101/gad.1870310

    authors： Zheng X,Mann RK,Sever N,Beachy PA

    更新日期：2010-01-01 00:00:00

  • Persistent initiation of DNA replication and chromatin-bound MCM proteins during the cell cycle in cdc6 mutants.

    abstract：:Faithful inheritance of genetic information requires that DNA be copied only once each cell cycle. Initiation of DNA replication involves the establishment of a prereplication complex (pre-RC) and subsequent activation by CDK/cyclins, converting the pre-RC to a post-RC. The origin recognition complex (ORC), Cdc6p, and...

    journal_title：Genes & development

    pub_type： 杂志文章

    doi：10.1101/gad.11.24.3375

    authors： Liang C,Stillman B

    更新日期：1997-12-15 00:00:00

  • Masquerade: a novel secreted serine protease-like molecule is required for somatic muscle attachment in the Drosophila embryo.

    abstract：:Diverse developmental processes, such as neuronal growth cone migration and cell shape changes, are mediated by the interactions of cells with the extracellular matrix. We describe here a secreted molecule encoded by the Drosophila masquerade (mas) gene. Total loss of mas function causes defective muscle attachment. T...

    journal_title：Genes & development

    pub_type： 杂志文章

    doi：10.1101/gad.9.2.139

    authors： Murugasu-Oei B,Rodrigues V,Yang X,Chia W

    更新日期：1995-01-15 00:00:00

  • Addicted to Myc--but why?

    abstract：:Many tumors continuously depend on the initiating oncogenes, but whether this extends to their downstream targets is unclear. In this issue of Genes & Development, Sodir and colleagues (pp. 907-916) demonstrate an essential role for endogenous Myc proteins in maintaining the tumor microenvironment, providing an unexpe...

    journal_title：Genes & development

    pub_type： 评论,杂志文章

    doi：10.1101/gad.2053311

    authors： von Eyss B,Eilers M

    更新日期：2011-05-01 00:00:00

  • A novel mechanism for telomere size control in Saccharomyces cerevisiae.

    abstract：:One of the central requirements for eukaryotic chromosome stability is the maintenance of the simple sequence tracts at telomeres. In this study, we use genetic and physical assays to reveal the nature of a novel mechanism by which telomere length is controlled. This mechanism, telomeric rapid deletion (TRD), is capab...

    journal_title：Genes & development

    pub_type： 杂志文章

    doi：10.1101/gad.10.11.1310

    authors： Li B,Lustig AJ

    更新日期：1996-06-01 00:00:00

  • A novel homeobox gene mediates the Dpp signal to establish functional specificity within target cells.

    abstract：:Morphogen gradients of secreted molecules play critical roles in the establishment of the spatial pattern of gene expression. During midgut development in Drosophila, secreted molecules of Decapentaplegic (Dpp) and Wingless (Wg) establish unique transcriptional regulation within target cells to specify the resultant c...

    journal_title：Genes & development

    pub_type： 杂志文章

    doi：10.1101/gad.12.17.2724

    authors： Nakagoshi H,Hoshi M,Nabeshima Y,Matsuzaki F

    更新日期：1998-09-01 00:00:00

  • The ClpXP and ClpAP proteases degrade proteins with carboxy-terminal peptide tails added by the SsrA-tagging system.

    abstract：:Interruption of translation in Escherichia coli can lead to the addition of an 11-residue carboxy-terminal peptide tail to the nascent chain. This modification is mediated by SsrA RNA (also called 10Sa RNA and tmRNA) and marks the tagged polypeptide for proteolysis. Degradation in vivo of lambda repressor amino-termin...

    journal_title：Genes & development

    pub_type： 杂志文章

    doi：10.1101/gad.12.9.1338

    authors： Gottesman S,Roche E,Zhou Y,Sauer RT

    更新日期：1998-05-01 00:00:00