Abstract:
:In eubacteria, the ClpS adaptor has been proposed to be essential for degradation of N-end rule substrates by the AAA(+) protease ClpAP. To test this model, we assayed degradation of substrates bearing N-end rule sequences isolated in a genetic screen for efficient degradation tags. ClpS was not vital for degradation in vivo but rather stimulated turnover in a sequence-specific manner. Although ClpS substantially enhanced degradation of N-end substrates at low substrate concentrations in vitro, it suppressed the degradation rate when substrate was saturating. Thus, we conclude that ClpAP recognizes N-end rule substrates directly, whereas ClpS modulates this degradation pathway.
journal_name
Genes Devjournal_title
Genes & developmentauthors
Wang KH,Sauer RT,Baker TAdoi
10.1101/gad.1511907subject
Has Abstractpub_date
2007-02-15 00:00:00pages
403-8issue
4eissn
0890-9369issn
1549-5477pii
21/4/403journal_volume
21pub_type
杂志文章abstract::The ETS transcription factors perform distinct biological functions despite conserving a highly similar DNA-binding domain. One distinguishing property of a subset of ETS proteins is a conserved region of 80 amino acids termed the Pointed (PNT) domain. Using enzyme kinetics we determined that the Ets-1 PNT domain cont...
journal_title:Genes & development
pub_type: 杂志文章
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journal_title:Genes & development
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pub_type: 杂志文章
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journal_title:Genes & development
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