Abstract:
:Caenorhabditis elegans let-7, a founding member of the microRNA family, is predicted to bind to six sites in the 3'UTR of the mRNA of its target gene, lin-41, to down-regulate LIN-41. Here, we demonstrate that wild-type let-7 microRNA binds in vitro to RNA from the lin-41 3'UTR. This interaction is dependent on two conserved let-7 complementary sites (LCSs). A 27-nucleotide sequence between the LCSs is also necessary for down-regulation in vivo. LCS mutations compensatory to the lesion in let-7(n2853) can partially restore lin-41 3'UTR function in a let-7(n2853) background, providing the first experimental evidence for an animal miRNA binding directly to its validated target in vivo.
journal_name
Genes Devjournal_title
Genes & developmentauthors
Vella MC,Choi EY,Lin SY,Reinert K,Slack FJdoi
10.1101/gad.1165404subject
Has Abstractpub_date
2004-01-15 00:00:00pages
132-7issue
2eissn
0890-9369issn
1549-5477pii
1165404journal_volume
18pub_type
杂志文章abstract::The Drosophila somatic sex-determination regulatory pathway has been well studied, but little is known about the target genes that it ultimately controls. In a differential screen for sex-specific transcripts expressed in fly heads, we identified a highly male-enriched transcript encoding Takeout, a protein related to...
journal_title:Genes & development
pub_type: 杂志文章
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abstract::Serotonin is a bioamine regulating bone mass accrual differently depending on its site of synthesis. It decreases accrual when synthesized in the gut, and increases it when synthesized in the brain. The signal transduction events elicited by gut-derived serotonin once it binds to the Htr1b receptor present on osteobla...
journal_title:Genes & development
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journal_title:Genes & development
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pub_type: 杂志文章
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journal_title:Genes & development
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journal_title:Genes & development
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journal_title:Genes & development
pub_type: 评论,杂志文章
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pub_type: 杂志文章
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pub_type: 杂志文章,评审
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journal_title:Genes & development
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journal_title:Genes & development
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journal_title:Genes & development
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journal_title:Genes & development
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