Abstract：
:In the cytoplasm of oocytes and early embryos, addition of poly(A) to mRNAs can activate their translation. We demonstrate that despite many differences between poly(A) addition in the cytoplasm and nucleus, these two forms of polyadenylation may involve identical trans-acting factors. Nuclear polyadenylation requires the sequence AAUAAA, the AAUAAA-binding cleavage and polyadenylation specificity factor (CPSF), and a poly(A) polymerase (PAP). We show that CPSF and PAP, purified from calf thymus, exhibit the same sequence specificity observed in the cytoplasm during frog oocyte maturation, requiring both AAUAAA and a proximal U-rich sequence. The enhanced polyadenylation of RNAs containing U-rich sequences is caused by their increased affinity for CPSF. Frog nuclear polyadenylation factors display cytoplasmic sequence specificity when dilute, suggesting that a difference in their concentrations in the nucleus and cytoplasm underlies the different sequence specificities in the two compartments. Because polyadenylation in extracts prepared from oocytes before maturation is stimulated by addition of CPSF, the onset of polyadenylation during early development may be attributable to the activation or synthesis of a CPSF-like factor. We suggest that sequences upstream of AAUAAA that are required for cleavage and polyadenylation of certain pre-mRNAs in the nucleus may be functionally equivalent to the upstream, U-rich sequences that function in the cytoplasm, enhancing CPSF binding. We propose that CPSF and PAP comprise a core polyadenylation apparatus in the cytoplasm of oocytes and early embryos.
journal_name
Genes Devjournal_title
Genes & developmentauthors
Bilger A,Fox CA,Wahle E,Wickens Mdoi
10.1101/gad.8.9.1106subject
Has Abstractpub_date
1994-05-01 00:00:00pages
1106-16issue
9eissn
0890-9369issn
1549-5477journal_volume
8pub_type
杂志文章abstract：:Heparan sulfate proteoglycans have been implicated in the presentation of a number of secreted signaling molecules to their signal-transducing receptors. We have characterized a gene trap mutation in the gene encoding a heparan sulfate biosynthetic enzyme, heparan sulfate 2-sulfotransferase (HS2ST). Transgenic mice we...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.12.12.1894
更新日期：1998-06-15 00:00:00
abstract：:NF-E1, a DNA-binding protein that recognizes the general consensus motif WGATAR, is the first tissue-specific factor to be identified in erythroid cells. Using a probe from the murine GF-1 (NF-E1) cDNA clone, we isolated three homologous chicken cDNAs: One of these corresponds to an mRNA (NF-E1a) that is abundantly an...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.4.10.1650
更新日期：1990-10-01 00:00:00
abstract：:RNA polymerase II nascent transcripts are capped during pausing before elongation. Here we report that hSPT5, the human homolog of yeast elongation factor SPT5, interacts directly with the capping enzyme. hSPT5 stimulated capping enzyme guanylylation and mRNA capping by severalfold. Although RNA 5'-triphosphatase acti...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.13.14.1774
更新日期：1999-07-15 00:00:00
abstract：:TFIID is a multisubunit protein containing the TATA box-binding polypeptide (TBP) and associated factors (TFIID-TAFs) required for activated transcription by RNA polymerase II. TBPs from different eukaryotes contain a highly conserved carboxy-terminal domain and very divergent amino-terminal domains. Earlier studies p...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.7.2.180
更新日期：1993-02-01 00:00:00
abstract：:Epigenetic modifications of chromatin play an important role in the regulation of gene expression. KMT4/Dot1 is a conserved histone methyltransferase capable of methylating chromatin on Lys79 of histone H3 (H3K79). Here we report the identification of a multisubunit Dot1 complex (DotCom), which includes several of the...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.1898410
更新日期：2010-03-15 00:00:00
abstract：:Mismatch repair (MMR) has important roles in meiotic and mitotic recombination, DNA damage signaling, and various aspects of DNA metabolism including class-switch recombination, somatic hypermutation, and triplet-repeat expansion. Defects in MMR are responsible for human cancers characterized by microsatellite instabi...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.430107
更新日期：2007-09-01 00:00:00
abstract：:Over a period of many months, Xenopus oocytes stockpile large quantities of tRNA for use during the first few hours of embryogenesis. To test the idea that these tRNAs are transcribed from one set of genes and that another set is used by somatic cells, we used synthetic oligonucleotides to analyze the sequence and ste...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.3.8.1190
更新日期：1989-08-01 00:00:00
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journal_title：Genes & development
pub_type： 杂志文章,评审
doi：10.1101/gad.1437206
更新日期：2006-08-15 00:00:00
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journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.339978.120
更新日期：2020-10-01 00:00:00
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journal_title：Genes & development
pub_type： 杂志文章
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更新日期：2012-04-01 00:00:00
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journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.3.6.770
更新日期：1989-06-01 00:00:00
abstract：:Sonic hedgehog (Shh) signals via Gli transcription factors to direct digit number and identity in the vertebrate limb. We characterized the Gli-dependent cis-regulatory network through a combination of whole-genome chromatin immunoprecipitation (ChIP)-on-chip and transcriptional profiling of the developing mouse limb....
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.1693008
更新日期：2008-10-01 00:00:00
abstract：:Establishment of the steroid-producing Leydig cell lineage is an event downstream of Sry that is critical for masculinization of mammalian embryos. Neither the origin of fetal Leydig cell precursors nor the signaling pathway that specifies the Leydig cell lineage is known. Based on the sex-specific expression patterns...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.981202
更新日期：2002-06-01 00:00:00
abstract：:rhomboid (rho) belongs to a group of four genes involved in the elaboration of positional information at a ventrolateral level in the Drosophila embryo. Mutations at any of these four loci also lead to a variety of other phenotypes, including reduction in the number of stretch receptor organs (chordotonal organs) in t...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.4.2.190
更新日期：1990-02-01 00:00:00
abstract：:The Saccharomyces cerevisiae protein kinase Rim15p was identified previously as a stimulator of meiotic gene expression. Here, we show that loss of Rim15p causes an additional pleiotropic phenotype in cells grown to stationary phase on rich medium; this phenotype includes defects in trehalose and glycogen accumulation...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.12.18.2943
更新日期：1998-09-15 00:00:00
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journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.6.10.2010
更新日期：1992-10-01 00:00:00
abstract：:The female mammary gland is a very dynamic organ that undergoes continuous tissue remodeling during adulthood. Although it is well established that the number of menstrual cycles and pregnancy (in this case transiently) increase the risk of breast cancer, the reasons are unclear. Growing clinical and experimental evid...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.292318.116
更新日期：2017-03-15 00:00:00
abstract：:Hepatitis B virus (HBV) infects humans and causes a wide range of clinical manifestations, from acute hepatitis to hepatocellular carcinoma (HCC). The HBV genome contains multiple promoters with gene expression regulated predominantly by the cellular transcription initiation machinery. Accordingly, the HBV-encoded pX,...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.12.8.1217
更新日期：1998-04-15 00:00:00
abstract：:Tuberous sclerosis (TSC) is an autosomal dominant disease characterized by hamartoma formation in various organs and is caused by mutations targeting either the TSC1 or TSC2 genes. TSC1 and TSC2 proteins form a functionally interdependent dimeric complex. Phosphorylation of either TSC subunit by different kinases regu...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.1624008
更新日期：2008-04-01 00:00:00
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journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.1.1.47
更新日期：1987-03-01 00:00:00
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journal_title：Genes & development
pub_type： 杂志文章
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更新日期：2005-08-15 00:00:00
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journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.1413706
更新日期：2006-07-15 00:00:00
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journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.10.17.2158
更新日期：1996-09-01 00:00:00
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journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.917101
更新日期：2001-11-01 00:00:00
abstract：:Telomerase is a ribonucleoprotein enzyme that adds telomeric repeats to chromosomes, maintaining telomere length and stabilizing chromosome ends. In vitro, telomerase from the ciliate Tetrahymena elongates single-stranded, guanosine-rich DNA primers by adding repeats of the Tetrahymena telomeric sequence, dT2G4. We ha...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.7.7b.1364
更新日期：1993-07-01 00:00:00
abstract：:S-phase cyclin-dependent kinase Cdc28-Clb5 (CDK-S) and Dbf4-dependent kinase Cdc7-Dbf4 (DDK) are highly conserved kinases well known for their roles in the initiation of DNA replication. CDK-S is also essential for initiation of meiotic recombination because it phosphorylates Ser30 of Mer2, a meiosis-specific double-s...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.1626408
更新日期：2008-02-01 00:00:00
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journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.281203
更新日期：2003-12-01 00:00:00
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journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.1584307
更新日期：2007-11-15 00:00:00
abstract：:A core promoter element called an initiator (Inr) overlaps the transcription start site of numerous mammalian protein-coding genes. In promoters that lack a TATA box, the Inr is functionally analogous to TATA, in that it is capable of directing basal transcription by RNA polymerase II and of determining the precise si...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.8.7.821
更新日期：1994-04-01 00:00:00
abstract：:Entry into sporulation by the Gram-positive bacterium Bacillus subtilis is governed by two transcription factors, Spo0A and sigma H, and involves a switch in the site of division from a medial to a polar location. We report that at the onset of sporulation, assembly of the cell division protein FtsZ shifts from midcel...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.10.4.478
更新日期：1996-02-15 00:00:00
