The transcriptional activator PAX3-FKHR rescues the defects of Pax3 mutant mice but induces a myogenic gain-of-function phenotype with ligand-independent activation of Met signaling in vivo.

abstract：:RNA interference (RNAi) is a flexible gene silencing mechanism that responds to double-stranded RNA by suppressing homologous genes. Here, we report the characterization of RNAi effector complexes (RISCs) that contain small interfering RNAs and microRNAs (miRNAs). We identify two putative RNA-binding proteins, the Dro...

journal_title：Genes & development

authors： Caudy AA,Myers M,Hannon GJ,Hammond SM

更新日期：2002-10-01 00:00:00

abstract：:The senescence-associated secretory phenotype (SASP) is a major trait of senescent cells, but the molecular regulators of SASP factor secretion are poorly understood. Mass spectrometry analysis revealed that secretory carrier membrane protein 4 (SCAMP4) levels were strikingly elevated on the surface of senescent cells...

journal_title：Genes & development

authors： Kim KM,Noh JH,Bodogai M,Martindale JL,Pandey PR,Yang X,Biragyn A,Abdelmohsen K,Gorospe M

更新日期：2018-07-01 00:00:00

abstract：:Start sites of DNA replication are marked by the origin recognition complex (ORC), which coordinates Mcm2-7 helicase loading to form the prereplicative complex (pre-RC). Although pre-RC assembly is well characterized in vitro, the process is poorly understood within the local chromatin environment surrounding replicat...

journal_title：Genes & development

authors： Belsky JA,MacAlpine HK,Lubelsky Y,Hartemink AJ,MacAlpine DM

更新日期：2015-01-15 00:00:00

abstract：:Morphogenesis of the insect kidney, the Malpighian tubules, is controlled in Drosophila by a single large cell, the tip cell. It has been postulated that this cell sends out a mitogenic signal that induces the division of neighboring cells. The signal and the molecules that receive it have remained elusive. We show th...

journal_title：Genes & development

authors： Kerber B,Fellert S,Hoch M

更新日期：1998-06-15 00:00:00

abstract：:Here we show that the cell cycle defects of dE2F1-depleted cells depend on the cooperative effects of dE2F2 and DACAPO (DAP), an inhibitor of Cyclin E/cyclin-dependent kinase 2 (CycE/cdk2). The different properties of cells lacking dE2F1/dE2F2 and dE2F1/DAP lead to the surprising observation that dE2F2-mediated repres...

journal_title：Genes & development

authors： Frolov MV,Stevaux O,Moon NS,Dimova D,Kwon EJ,Morris EJ,Dyson NJ

更新日期：2003-03-15 00:00:00

abstract：:Apoptosis is an important mechanism to eliminate potentially tumorigenic cells. The tumor suppressor p53 plays a pivotal role in this process. Many tumors harbor mutant p53, but others evade its tumor-suppressive effects by altering the expression of proteins that regulate the p53 pathway. ASPP1 (apoptosis-stimulating...

journal_title：Genes & development

authors： Aylon Y,Ofir-Rosenfeld Y,Yabuta N,Lapi E,Nojima H,Lu X,Oren M

更新日期：2010-11-01 00:00:00

abstract：:Glucose and glutamine serve as the two primary carbon sources in proliferating cells, and uptake of both nutrients is directed by growth factor signaling. Although either glucose or glutamine can potentially support mitochondrial tricarboxylic acid (TCA) cycle integrity and ATP production, we found that glucose depriv...

journal_title：Genes & development

authors： Wellen KE,Lu C,Mancuso A,Lemons JM,Ryczko M,Dennis JW,Rabinowitz JD,Coller HA,Thompson CB

更新日期：2010-12-15 00:00:00

abstract：:Pancreatic ductal adenocarcinoma (PDAC) is the fourth leading cause of cancer death in the United States with a median survival of <6 mo and a dismal 5-yr survival rate of 3%-5%. The cancer's lethal nature stems from its propensity to rapidly disseminate to the lymphatic system and distant organs. This aggressive biol...

journal_title：Genes & development

authors： Hezel AF,Kimmelman AC,Stanger BZ,Bardeesy N,Depinho RA

更新日期：2006-05-15 00:00:00

abstract：:Numerous lines of study have suggested that the Hox genes, encoding putative transcription factors, are key genes in the establishment of the body plan of the mammalian embryo. To examine the role of Hoxa-5 (Hox1.3) gene during development, we have used targeted mutagenesis in embryonic stem cells to produce a strain ...

journal_title：Genes & development

authors： Jeannotte L,Lemieux M,Charron J,Poirier F,Robertson EJ

更新日期：1993-11-01 00:00:00

abstract：:Hedgehog signaling has been linked to cell proliferation in a variety of systems; however, its effects on the cell cycle have not been closely studied. In the vertebrate retina, Hedgehog's effects are controversial, with some reports emphasizing increased proliferation and others pointing to a role in cell cycle exit....

journal_title：Genes & development

authors： Locker M,Agathocleous M,Amato MA,Parain K,Harris WA,Perron M

更新日期：2006-11-01 00:00:00

abstract：:Gene inactivation can result when a delta element of the Ty1 transposon inserted into the yeast HIS4 promoter (his4-912delta) alters the transcription initiation site. Previous work has identified mutations that suppress this transcriptional defect by restoring the transcription start site to the native position, and ...

journal_title：Genes & development

authors： Jiang YW,Stillman DJ

更新日期：1996-03-01 00:00:00

abstract：:Accurate and regulated transcription by RNA polymerase II requires the assembly of an initiation complex involving multiple protein-DNA and protein-protein interactions. A key event is binding of TFIID, a complex consisting of TBP and associated factors (TAFs) to the template DNA. The TAF subunits of TFIID carry out d...

journal_title：Genes & development

authors： Ruppert S,Tjian R

更新日期：1995-11-15 00:00:00

abstract：:Interactions required for inhibition of poly(A) polymerase (PAP) by the U1 snRNP-specific U1A protein, a reaction whose function is to autoregulate U1A protein production, are examined. PAP inhibition requires a substrate RNA to which at least two molecules of U1A protein can bind tightly, but we demonstrate that the ...

journal_title：Genes & development

authors： Gunderson SI,Vagner S,Polycarpou-Schwarz M,Mattaj IW

更新日期：1997-03-15 00:00:00

abstract：:Adenomatous polyposis coli (APC) regulates the activity of β-catenin, an integral component of Wnt signaling. However, the selective role of the APC-β-catenin pathway in cerebral cortical development is unknown. Here we genetically dissected the relative contributions of APC-regulated β-catenin signaling in cortical p...

journal_title：Genes & development

authors： Nakagawa N,Li J,Yabuno-Nakagawa K,Eom TY,Cowles M,Mapp T,Taylor R,Anton ES

更新日期：2017-08-15 00:00:00

abstract：:Transcriptional profiling is a powerful approach for understanding development and disease. Current cell type-specific RNA purification methods have limitations, including cell dissociation trauma or inability to identify all RNA species. Here, we describe "mouse thiouracil (TU) tagging," a genetic and chemical inters...

journal_title：Genes & development

authors： Gay L,Miller MR,Ventura PB,Devasthali V,Vue Z,Thompson HL,Temple S,Zong H,Cleary MD,Stankunas K,Doe CQ

更新日期：2013-01-01 00:00:00

abstract：:DNA methylation is tightly regulated throughout mammalian development, and altered DNA methylation patterns are a general hallmark of cancer. The methylcytosine dioxygenase TET2 is frequently mutated in hematological disorders, including acute myeloid leukemia (AML), and has been suggested to protect CG dinucleotide (...

journal_title：Genes & development

authors： Rasmussen KD,Jia G,Johansen JV,Pedersen MT,Rapin N,Bagger FO,Porse BT,Bernard OA,Christensen J,Helin K

更新日期：2015-05-01 00:00:00

abstract：:Nonsense-mediated mRNA decay (NMD) is a quality control mechanism that detects and degrades mRNAs containing premature stop codons (PTCs). In vertebrates, PTCs trigger efficient NMD when located upstream of an exon junction complex (EJC). Degradation of PTC-containing mRNAs requires the endonucleolytic activity of SMG...

journal_title：Genes & development

authors： Kashima I,Jonas S,Jayachandran U,Buchwald G,Conti E,Lupas AN,Izaurralde E

更新日期：2010-11-01 00:00:00

abstract：:Binding of microRNA (miRNA) to mRNA within the RNA-induced silencing complex (RISC) leads to either translational inhibition or to destruction of the target mRNA. Both of these functions are executed by Argonaute 2 (Ago2). Using hematopoiesis in mice as a model system to study the physiological function of Ago2 in viv...

journal_title：Genes & development

authors： O'Carroll D,Mecklenbrauker I,Das PP,Santana A,Koenig U,Enright AJ,Miska EA,Tarakhovsky A

更新日期：2007-08-15 00:00:00

abstract：:We demonstrate here that the previously described bacterial promoter upstream element (UP element) consists of two distinct subsites, each of which, by itself, can bind the RNA polymerase holoenzyme alpha subunit carboxy-terminal domain (RNAP alphaCTD) and stimulate transcription. Using binding-site-selection experime...

journal_title：Genes & development

authors： Estrem ST,Ross W,Gaal T,Chen ZW,Niu W,Ebright RH,Gourse RL

更新日期：1999-08-15 00:00:00

abstract：:Organization into polarized three-dimensional structures defines whether epithelial cells are normal or malignant. In a model of morphogenesis, we show that inhibiting key signaling pathways in human breast cancer cells leads to "phenotypic reversion" of the malignant cells. Using architecture as an endpoint, we repor...

journal_title：Genes & development

authors： Beliveau A,Mott JD,Lo A,Chen EI,Koller AA,Yaswen P,Muschler J,Bissell MJ

更新日期：2010-12-15 00:00:00

abstract：:Genomic imprinting is an epigenetic mechanism by which genes are expressed in a parental origin-dependent manner. We recently discovered that, like DNA methylation, oocyte-inherited H3K27me3 can also serve as an imprinting mark in mouse preimplantation embryos. In this study, we found H3K27me3 is strongly biased towar...

journal_title：Genes & development

authors： Zhang W,Chen Z,Yin Q,Zhang D,Racowsky C,Zhang Y

更新日期：2019-04-01 00:00:00

abstract：:The mechanisms of RNA replication of plus-strand RNA viruses are still unclear. Here, we identified the first promoter element for RNA synthesis described in a flavivirus. Using dengue virus as a model, we found that the viral RdRp discriminates the viral RNA by specific recognition of a 5' element named SLA. We demon...

journal_title：Genes & development

authors： Filomatori CV,Lodeiro MF,Alvarez DE,Samsa MM,Pietrasanta L,Gamarnik AV

更新日期：2006-08-15 00:00:00

abstract：:MicroRNAs (miRNAs) are approximately 22-nucleotide RNAs that are processed from characteristic precursor hairpins and pair to sites in messages of protein-coding genes to direct post-transcriptional repression. Here, we report that the miRNA iab-4 locus in the Drosophila Hox cluster is transcribed convergently from bo...

journal_title：Genes & development

authors： Stark A,Bushati N,Jan CH,Kheradpour P,Hodges E,Brennecke J,Bartel DP,Cohen SM,Kellis M

更新日期：2008-01-01 00:00:00

abstract：:Genome integrity is constantly monitored by sophisticated cellular networks, collectively termed the DNA damage response (DDR). A common feature of DDR proteins is their mobilization in response to genotoxic stress. Here, we outline how the development of various complementary methodologies has provided valuable insig...

journal_title：Genes & development

authors： Polo SE,Jackson SP

更新日期：2011-03-01 00:00:00

abstract：:Maternal imprinting at the Xist gene is essential to achieve paternal allele-specific imprinted X-chromosome inactivation (XCI) in female mammals. However, the mechanism underlying Xist imprinting is unclear. Here we show that the Xist locus is coated with a broad H3K27me3 domain that is established during oocyte grow...

journal_title：Genes & development

authors： Inoue A,Jiang L,Lu F,Zhang Y

更新日期：2017-10-01 00:00:00

abstract：:The nonsense-mediated mRNA decay (NMD) pathway selectively degrades mRNAs harboring premature termination codons (PTCs). Seven genes (smg-1-7, for suppressor with morphological effect on genitalia) that are essential for NMD were originally identified in the nematode Caenorhabditis elegans, and orthologs of these gene...

journal_title：Genes & development

authors： Longman D,Plasterk RH,Johnstone IL,Cáceres JF

更新日期：2007-05-01 00:00:00

abstract：:The gamma delta T-cell receptor (TCR) is the first TCR to be expressed in ontogeny in all vertebrates in which it has been examined thoroughly. Murine gamma delta cell-surface protein is detected by the fourteenth day of gestation. In this work, the activation of gamma delta RNA has been studied. Data indicate that th...

journal_title：Genes & development

authors： Carding SR,Kyes S,Jenkinson EJ,Kingston R,Bottomly K,Owen JJ,Hayday AC

更新日期：1990-08-01 00:00:00

abstract：:The molecular mechanisms driving brain development at risk in autism spectrum disorders (ASDs) remain mostly unknown. Previous studies have implicated the transcription factor FOXP1 in both brain development and ASD pathophysiology. However, the specific molecular pathways both upstream of and downstream from FOXP1 ar...

journal_title：Genes & development

authors： Usui N,Araujo DJ,Kulkarni A,Co M,Ellegood J,Harper M,Toriumi K,Lerch JP,Konopka G

更新日期：2017-10-15 00:00:00

abstract：:Neurofibromatosis type 1 (NF1) is a common neurodevelopmental disorder caused by impaired function of the neurofibromin RAS regulator. Using a combination of Nf1 genetically engineered mice and pharmacological/genetic inhibition approaches, we report that neurofibromin differentially controls neural stem cell (NSC) pr...

journal_title：Genes & development

authors： Chen YH,Gianino SM,Gutmann DH

更新日期：2015-08-15 00:00:00

abstract：:A euchromatic gene placed in the vicinity of heterochromatin by a chromosomal rearrangement generally exhibits position effect variegation (PEV), a clonally inherited pattern showing gene expression in some somatic cells but not in others. The mechanism responsible for this loss of gene expression is investigated here...

journal_title：Genes & development

authors： Wallrath LL,Elgin SC

更新日期：1995-05-15 00:00:00