Abstract：
:Heparan sulfate proteoglycans have been implicated in the presentation of a number of secreted signaling molecules to their signal-transducing receptors. We have characterized a gene trap mutation in the gene encoding a heparan sulfate biosynthetic enzyme, heparan sulfate 2-sulfotransferase (HS2ST). Transgenic mice were generated from embryonic stem cells harboring this insertion. lacZ reporter gene activity in heterozygous embryos demonstrates that the gene is expressed differentially during embryogenesis, presumably directing dynamic changes in heparan sulfate structure. Moreover, mice homozygous for the Hs2st gene trap allele die in the neonatal period, exhibiting bilateral renal agenesis and defects of the eye and the skeleton. Analysis of kidney development in Hs2st mutants reveals that the gene is not required for two early events-ureteric bud outgrowth from the Wolffian duct and initial induction of Pax-2 expression in the metanephric mesenchyme. It is required, however, for mesenchymal condensation around the ureteric bud and initiation of branching morphogenesis. Because 2-O-sulfation has been shown to influence the functional interactions of ligands with heparan sulfate in vitro, we discuss the possibility that the Hs2st mutant phenotype is a consequence of compromised interactions between growth factors and their signal-transducing receptors. These data provide the first genetic evidence that the regulated synthesis of differentially glycosylated proteoglycans can affect morphogenesis during vertebrate development.
journal_name
Genes Devjournal_title
Genes & developmentauthors
Bullock SL,Fletcher JM,Beddington RS,Wilson VAdoi
10.1101/gad.12.12.1894subject
Has Abstractpub_date
1998-06-15 00:00:00pages
1894-906issue
12eissn
0890-9369issn
1549-5477journal_volume
12pub_type
杂志文章abstract：:Cdc42p, a Rho-related GTP-binding protein, regulates cytoskeletal polarization and rearrangements in eukaryotic cells, but the effectors mediating this control remain unknown. Through the use of the complete yeast genomic sequence, we have identified two novel Cdc42p targets, Gic1p and Gic2p, which contain consensus C...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.11.22.2972
更新日期：1997-11-15 00:00:00
abstract：:In this issue of Genes & Development, Abruzzi et al. (pp. 2374-2386) use chromatin immunoprecipitation (ChIP) tiling array assays (ChIP-chip) to show that physical interactions between circadian (≅24-h) clock machineries and genomes are more widespread than previously thought and provide novel insights into how clocks...
journal_title：Genes & development
pub_type： 评论,杂志文章
doi：10.1101/gad.180984.111
更新日期：2011-11-15 00:00:00
abstract：:Intracellular heme levels must be tightly regulated to maintain proper mitochondrial respiration while minimizing toxicity, but the homeostatic mechanisms are not well understood. Here we report a novel negative feedback mechanism whereby the nuclear heme receptor Rev-erbalpha tightly controls the level of its own lig...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.1825809
更新日期：2009-09-15 00:00:00
abstract：:AMP-activated protein kinase (AMPK) is a sensor of energy status that maintains cellular energy homeostasis. It arose very early during eukaryotic evolution, and its ancestral role may have been in the response to starvation. Recent work shows that the kinase is activated by increases not only in AMP, but also in ADP....
journal_title：Genes & development
pub_type： 杂志文章,评审
doi：10.1101/gad.17420111
更新日期：2011-09-15 00:00:00
abstract：:Signals from TGF-beta superfamily receptors are transduced to the nucleus by Smad proteins, which transcriptionally activate target genes. In Caenorhabditis elegans, defects in a TGF-beta-related pathway cause a reversible developmental arrest and metabolic shift at the dauer larval stage. Null mutations in daf-3 supp...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.11.20.2679
更新日期：1997-10-15 00:00:00
abstract：:Previous work has shown that rRNA synthesis is strongly inhibited in yeast top1-top2 double mutants. Here, we show that inactivation of yeast topoisomerases can have paradoxical effects on transcription by RNA polymerase I. For example, transcription of ribosomal minigenes on extrachromosomal plasmids is greatly stimu...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.6.7.1332
更新日期：1992-07-01 00:00:00
abstract：:The presence of Set2-mediated methylation of H3K36 (K36me) correlates with transcription frequency throughout the yeast genome. K36me targets the Rpd3S complex to deacetylate transcribed regions and suppress cryptic transcription initiation at certain genes. Here, using a genome-wide approach, we report that the Set2-...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.1539307
更新日期：2007-06-01 00:00:00
abstract：:Mammalian cells exhibit a complex response to DNA damage. The tumor suppressor BRCA1 and associated protein BARD1 are thought to play an important role in this response, and our previous work demonstrated that this includes transient inhibition of the pre-mRNA 3' processing machinery. Here we provide evidence that thi...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.1309505
更新日期：2005-05-15 00:00:00
abstract：:We generated and characterized greater than 500 Drosophila strains that carry single copies of a novel P-element enhancer detector. In the majority of the strains, the beta-galactosidase reporter gene in the P-transposon responds to nearby transcriptional regulatory sequences in the genome. A remarkable diversity of s...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.3.9.1288
更新日期：1989-09-01 00:00:00
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journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.9.10.1263
更新日期：1995-05-15 00:00:00
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journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.1366405
更新日期：2005-12-01 00:00:00
abstract：:The Neurospora crassa clock-controlled gene (ccg-2) is transcriptionally activated by the circadian clock in a time-of-day-specific manner. Transcript and sequence analyses of ccg-2 reveal that the predicted Ccg-2 polypeptide bears significant similarity to a class of low-molecular-weight, cysteine-rich, hydrophobic p...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.6.12a.2382
更新日期：1992-12-01 00:00:00
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journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.9.4.471
更新日期：1995-02-15 00:00:00
abstract：:Adult mesenchymal stem cells (MSCs) derived from bone marrow contribute to the regeneration of multiple types of mesenchymal tissues. Here we describe the functional role of a novel form of cross-talk between the transforming growth factor beta1 (TGF-beta1) and Wnt signaling pathways in regulating the activities of hu...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.1388806
更新日期：2006-03-15 00:00:00
abstract：:The amino-terminal histone tails are subject to covalent post-translational modifications such as acetylation, methylation, and phosphorylation. In the histone code hypothesis, these exposed and unstructured histone tails are accessible to a repertoire of regulatory factors that specifically recognize the various modi...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.1001502
更新日期：2002-06-15 00:00:00
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journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.184051.111
更新日期：2012-05-15 00:00:00
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journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.10.8.921
更新日期：1996-04-15 00:00:00
abstract：:We have analyzed pre-mRNA splicing in yeast extracts immunodepleted of the PRP18 protein. We find that while the first step of splicing (cleavage at the 5' splice site, and generation of the exon 1 and lariat intermediates) is unaffected by the absence of PRP18, the second step of splicing (excision of the lariat intr...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.7.2.320
更新日期：1993-02-01 00:00:00
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journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.5.5.728
更新日期：1991-05-01 00:00:00
abstract：:TDP-43 is a critical RNA-binding factor associated with pre-mRNA splicing in mammals. Its expression is tightly autoregulated, with loss of this regulation implicated in human neuropathology. We demonstrate that TDP-43 overexpression in humans and mice activates a 3' untranslated region (UTR) intron, resulting in exci...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.194829.112
更新日期：2012-08-01 00:00:00
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journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.178962.111
更新日期：2011-12-01 00:00:00
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journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.212738.112
更新日期：2013-03-15 00:00:00
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journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.1252404
更新日期：2004-12-15 00:00:00
abstract：:An RNA-binding protein gene (rbp1) from Drosophila melanogaster, encoding an RNA recognition motif and an Arg-Ser rich (RS) domain, has been characterized. The predicted amino acid sequence of rbp1 is similar to those of the human splicing factor ASF/SF2, the Drosophila nuclear phosphoprotein SRp55, and the Drosophila...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.6.12b.2569
更新日期：1992-12-01 00:00:00
abstract：:The mouse Nedd5 gene encodes a 41.5-kD GTPase similar to the Saccharomyces and Drosophila septins essential for cytokinesis. Nedd5 accumulates near the contractile ring from anaphase through telophase, and finally condenses into the midbody. Microinjection of anti-Nedd5 antibody interferes with cytokinesis, giving ris...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.11.12.1535
更新日期：1997-06-15 00:00:00
abstract：:Multiple DNA repair pathways maintain genome stability and ensure that DNA remains essentially unchanged over the life of a cell. Various human diseases occur if DNA repair is compromised, and most of these impact the nervous system, in some cases exclusively. However, it is often unclear what specific endogenous dama...
journal_title：Genes & development
pub_type： 杂志文章,评审
doi：10.1101/gad.301325.117
更新日期：2017-06-15 00:00:00
abstract：:A powerful enhancer has been mapped to an 18-bp DNA segment located 11 kb 5' to the human epsilon-globin gene within the dominant control or locus-activating region. This enhancer is inducible in K562 human erythroleukemia cells, increasing linked gamma-globin promoter/luciferase gene expression to 170-fold over an en...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.4.6.993
更新日期：1990-06-01 00:00:00
abstract：:We have developed a new technique, called 'toeprinting,' which has allowed a study of the tRNA-binding properties of Escherichia coli translation initiation complexes. In response to natural mRNAs, the initiator tRNA and a variety of elongator tRNAs bind to the same tRNA-binding site on the 30S ribosomal subunit as lo...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.3.12a.1899
更新日期：1989-12-01 00:00:00
abstract：:Previous studies have shown that differentiation of 3T3-L1 preadipocytes leads to the transcriptional activation of a group of adipose-specific genes. As an approach to defining the mechanism responsible for activating the expression of these genes, we investigated the binding of nuclear factors to the promoters of tw...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.3.9.1323
更新日期：1989-09-01 00:00:00
abstract：:P-element transformants of a single rRNA gene (rDNA) were used to investigate the relationship between the organization of the nucleolus organizer (NO) and rDNA function in Drosophila melanogaster. In situ hybridization to rRNA in polytene nuclei of salivary glands demonstrated that an rRNA gene can be transcribed at ...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.2.12b.1745
更新日期：1988-12-01 00:00:00
