Abstract:
:The catalytic RNA subunit (M1 RNA) of RNase P from Escherichia coli has been converted to an endoribonuclease that specifically cleaves the mRNA that encodes thymidine kinase (TK) of herpes simplex virus 1 (HSV-1). Covalent attachment to the 3' end of M1 RNA of a sequence complementary to TK mRNA results in very efficient cleavage of the target RNA in vitro. This reaction can be stimulated by proteins extracted from both E. coli and HeLa cells. When mouse cells in culture that express the novel RNA construct are infected with HSV-1, the levels of both TK mRNA and protein are reduced by approximately 80% as compared with cells that either do not express the novel RNA construct or express constructs with certain deletions that are known to abolish the catalytic activity of M1 RNA.
journal_name
Genes Devjournal_title
Genes & developmentauthors
Liu F,Altman Sdoi
10.1101/gad.9.4.471subject
Has Abstractpub_date
1995-02-15 00:00:00pages
471-80issue
4eissn
0890-9369issn
1549-5477journal_volume
9pub_type
杂志文章abstract::The Snail zinc-finger transcription factors trigger epithelial-mesenchymal transitions (EMTs), endowing epithelial cells with migratory and invasive properties during both embryonic development and tumor progression. During EMT, Snail provokes the loss of epithelial markers, as well as changes in cell shape and the ex...
journal_title:Genes & development
pub_type: 杂志文章
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journal_title:Genes & development
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pub_type: 杂志文章,评审
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journal_title:Genes & development
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journal_title:Genes & development
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journal_title:Genes & development
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journal_title:Genes & development
pub_type: 杂志文章
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journal_title:Genes & development
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journal_title:Genes & development
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更新日期:2001-12-15 00:00:00
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journal_title:Genes & development
pub_type: 杂志文章
doi:
更新日期:2000-06-01 00:00:00
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journal_title:Genes & development
pub_type: 杂志文章
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更新日期:2004-10-01 00:00:00
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journal_title:Genes & development
pub_type: 杂志文章
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更新日期:2013-09-15 00:00:00
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journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.243485.114
更新日期:2014-07-15 00:00:00
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pub_type: 评论,杂志文章
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journal_title:Genes & development
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更新日期:2016-03-01 00:00:00
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journal_title:Genes & development
pub_type: 杂志文章
doi:10.1101/gad.12.23.3639
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journal_title:Genes & development
pub_type: 杂志文章
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更新日期:2010-09-01 00:00:00
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journal_title:Genes & development
pub_type: 杂志文章
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更新日期:1992-04-01 00:00:00
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journal_title:Genes & development
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journal_title:Genes & development
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更新日期:2008-07-01 00:00:00
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journal_title:Genes & development
pub_type: 杂志文章,评审
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更新日期:2009-02-15 00:00:00
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journal_title:Genes & development
pub_type: 杂志文章
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