Abstract：
:The amino-terminal histone tails are subject to covalent post-translational modifications such as acetylation, methylation, and phosphorylation. In the histone code hypothesis, these exposed and unstructured histone tails are accessible to a repertoire of regulatory factors that specifically recognize the various modified histones, thereby generating altered chromatin structures that mediate specific biological responses. Here, we report that lysine (Lys) 79 of histone H3, which resides in the globular domain, is methylated in eukaryotic organisms. In the yeast Saccharomyces cerevisiae, Lys 79 of histone H3 is methylated by Dot1, a protein shown previously to play a role in telomeric silencing. Mutations of Lys 79 of histone H3 and mutations that abolish the catalytic activity of Dot1 impair telomeric silencing, suggesting that Dot1 mediates telomeric silencing largely through methylation of Lys 79. This defect in telomeric silencing might reflect an interaction between Sir proteins and Lys 79, because dot1 and Lys 79 mutations weaken the interaction of Sir2 and Sir3 with the telomeric region in vivo. Our results indicate that histone modifications in the core globular domain have important biological functions.
journal_name
Genes Devjournal_title
Genes & developmentauthors
Ng HH,Feng Q,Wang H,Erdjument-Bromage H,Tempst P,Zhang Y,Struhl Kdoi
10.1101/gad.1001502subject
Has Abstractpub_date
2002-06-15 00:00:00pages
1518-27issue
12eissn
0890-9369issn
1549-5477journal_volume
16pub_type
杂志文章abstract：:Unusually for a eukaryote, genes transcribed by RNA polymerase II (pol II) in Trypanosoma brucei are arranged in polycistronic transcription units. With one exception, no pol II promoter motifs have been identified, and how transcription is initiated remains an enigma. T. brucei has four histone variants: H2AZ, H2BV, ...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.1790409
更新日期：2009-05-01 00:00:00
abstract：:Chromatin is organized into higher-order structures that form subcompartments in interphase nuclei. Different categories of specialized enzymes act on chromatin and regulate its compaction and biophysical characteristics in response to physiological conditions. We present an overview of the function of chromatin struc...
journal_title：Genes & development
pub_type： 杂志文章,评审
doi：10.1101/gad.307702.117
更新日期：2017-11-15 00:00:00
abstract：:A transgenic mouse model for retinoblastoma was produced previously by directing SV40 T antigen expression to retinal photoreceptor cells using the promoter of the interstitial retinol-binding protein (IRBP) gene. This gene becomes active prior to the terminal differentiation of photoreceptors. Because T antigen-trans...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.8.11.1300
更新日期：1994-06-01 00:00:00
abstract：:Long noncoding RNAs (lncRNAs) can trigger repressive chromatin, but how they recruit silencing factors remains unclear. In Schizosaccharomyces pombe, heterochromatin assembly on transcribed noncoding pericentromeric repeats requires both RNAi and RNAi-independent mechanisms. In Saccharomyces cerevisiae, which lacks a ...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.226019.113
更新日期：2013-09-01 00:00:00
abstract：:Rb mutants exhibit aneuploidy and aberrant chromosome structure during mitosis. In this issue of Genes & Development, a new paper from Longworth and colleagues (1011-1024) describes both physical and functional interactions between Drosophila Rbf1 and the dCAP-D3 subunit of condensin II. This work directly implicates ...
journal_title：Genes & development
pub_type： 评论,杂志文章
doi：10.1101/gad.1666808
更新日期：2008-04-15 00:00:00
abstract：:Previous studies have shown that differentiation of 3T3-L1 preadipocytes leads to the transcriptional activation of a group of adipose-specific genes. As an approach to defining the mechanism responsible for activating the expression of these genes, we investigated the binding of nuclear factors to the promoters of tw...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.3.9.1323
更新日期：1989-09-01 00:00:00
abstract：:The activity of the polyoma virus (PyV) origin of DNA replication was used as a sensitive assay for enhancer function in one- and two-cell mouse embryos by injecting embryos with plasmid DNA containing different PyV ori configurations, allowing them to continue development in vitro, and then measuring plasmid DNA repl...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.2.9.1115
更新日期：1988-09-01 00:00:00
abstract：:Symbiotic nitrogen fixation involves the development of specialized organs called nodules within which plant photosynthates are exchanged for combined nitrogen of bacterial origin. To determine the importance of bacterial nitrogen metabolism in symbiosis, we have characterized a key regulator of this metabolism in Rhi...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.11.9.1194
更新日期：1997-05-01 00:00:00
abstract：:Organization into polarized three-dimensional structures defines whether epithelial cells are normal or malignant. In a model of morphogenesis, we show that inhibiting key signaling pathways in human breast cancer cells leads to "phenotypic reversion" of the malignant cells. Using architecture as an endpoint, we repor...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.1990410
更新日期：2010-12-15 00:00:00
abstract：:In a four-cell-stage Caenorhabditis elegans embryo, Wnt signaling polarizes an endoderm precursor called EMS. The polarization of this cell orients its mitotic spindle in addition to inducing endodermal fate in one daughter cell. Reducing the function of Wnt pathway genes, including a newly identified GSK-3beta homolo...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.13.15.2028
更新日期：1999-08-01 00:00:00
abstract：:Transcription of the cell-cycle-regulated human histone genes increases approximately fivefold during S phase. One step toward the elucidation of the biochemical mechanisms that govern cell-cycle-regulated expression of these genes is to purify and characterize the transcription factors that regulate these promoters. ...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.2.12b.1700
更新日期：1988-12-01 00:00:00
abstract：:Oxidative tissues such as heart undergo a dramatic perinatal mitochondrial biogenesis to meet the high-energy demands after birth. PPARgamma coactivator-1 (PGC-1) alpha and beta have been implicated in the transcriptional control of cellular energy metabolism. Mice with combined deficiency of PGC-1alpha and PGC-1beta ...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.1661708
更新日期：2008-07-15 00:00:00
abstract：:Upon activation by far-red light, phytochrome A signals are transduced through several pathways to promote photomorphogenesis. The COP1 E3 ligase represses photomorphogenesis in part by targeting transcription activators such as LAF1 and HY5 for destruction. Another positive regulator of photomorphogenesis is HFR1, a ...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.1247205
更新日期：2005-03-01 00:00:00
abstract：:The presence of Set2-mediated methylation of H3K36 (K36me) correlates with transcription frequency throughout the yeast genome. K36me targets the Rpd3S complex to deacetylate transcribed regions and suppress cryptic transcription initiation at certain genes. Here, using a genome-wide approach, we report that the Set2-...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.1539307
更新日期：2007-06-01 00:00:00
abstract：:Plectin, a major linker and scaffolding protein of the cytoskeleton, has been shown to be essential for the mechanical integrity of skin, skeletal muscle, and heart. Studying fibroblast and astroglial cell cultures derived from plectin (-/-) mice, we found that their actin cytoskeleton, including focal adhesion contac...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.12.21.3442
更新日期：1998-11-01 00:00:00
abstract：:The transcription factor Ebf1 is an important determinant of early B lymphopoiesis. To gain insight into the functions of Ebf1 at distinct stages of differentiation, we conditionally inactivated Ebf1. We found that Ebf1 is required for the proliferation, survival, and signaling of pro-B cells and peripheral B-cell sub...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.187328.112
更新日期：2012-04-01 00:00:00
abstract：:The unique property of stem cells to self-renew suggests specific mechanisms that regulate their cell-cycle progression. In the present study, we identify a novel protein, nucleostemin, found in the nucleoli of CNS stem cells, embryonic stem cells, and several cancer cell lines and preferentially expressed by other st...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.55671
更新日期：2002-12-01 00:00:00
abstract：:High-fidelity chromosome transmission is fundamental in controlling the quality of the cell division cycle. The spindle pole-to-pole distance remains constant from metaphase to anaphase A. We show that fission yeast sister centromere-connecting proteins, Mis6 and Mis12, are required for correct spindle morphogenesis, ...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.13.13.1664
更新日期：1999-07-01 00:00:00
abstract：:Endospores of Bacillus subtilis are encased in a two-layer protein shell known as the coat, which consists of a lammellar-like inner layer and an electron-dense outer layer. We report the cloning of the structural gene (designated cotE) for an alkali-soluble coat protein of 24 kD and show that the cotE gene product is...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.2.8.1047
更新日期：1988-08-01 00:00:00
abstract：:The contact site A glycoprotein is a developmentally regulated cell-surface component expressed during the aggregation stage of Dictyostelium discoideum. This protein has been implicated in the EDTA-stable (Ca2(+)-independent) type of cell adhesion of aggregating cells. The gene coding for the contact site A protein w...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.3.12a.2011
更新日期：1989-12-01 00:00:00
abstract：:The transcription factor TFIIB plays a central role in preinitiation complex assembly, providing a bridge between promoter-bound TFIID and RNA Polymerase II. TFIIB possesses sequence-specific DNA-binding ability and interacts with the TFIIB-recognition element (BRE), present in many promoters. Here we show that the BR...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.206901
更新日期：2001-11-15 00:00:00
abstract：:Classical embryology has provided a clear view of the timing and hormonal cues that govern sexual differentiation. Molecular biology has added important details to this picture. The cloning of SRY, MIS, and INSL3 provide insight into the molecular signals that provide important cues at the cellular level. Continued un...
journal_title：Genes & development
pub_type： 杂志文章,评审
doi：10.1101/gad.843800
更新日期：2000-12-15 00:00:00
abstract：:In the absence of growth signals, cells exit the cell cycle and enter into G0 or quiescence. Alternatively, cells enter senescence in response to inappropriate growth signals such as oncogene expression. The molecular mechanisms required for cell cycle exit into quiescence or senescence are poorly understood. The DREA...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.2034211
更新日期：2011-04-15 00:00:00
abstract：:The PWI motif is a highly conserved domain of unknown function in the SRm160 splicing and 3'-end cleavage-stimulatory factor, as well as in several other known or putative pre-mRNA processing components. We show here that the PWI motif is a new type of RNA/DNA-binding domain that has an equal preference for single- an...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.1060403
更新日期：2003-02-15 00:00:00
abstract：:The Hox gene products are transcription factors involved in specifying regional identity along the anteroposterior body axis. In the mouse, several single mutants for Hox genes show variably penetrant, partial homeotic transformations of vertebrae at their anterior limits of expression, suggesting that compound Hox mu...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.9.13.1667
更新日期：1995-07-01 00:00:00
abstract：:Discrete sequence elements known as exonic splicing enhancers (ESEs) have been shown to influence both the efficiency of splicing and the profile of mature mRNAs in multicellular eukaryotes. While the existence of ESEs has not been demonstrated previously in unicellular eukaryotes, the factors known to recognize these...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.1265905
更新日期：2005-01-15 00:00:00
abstract：:In cytoplasm, the survival of motor neuron (SMN) complex delivers pre-small nuclear RNAs (pre-snRNAs) to the heptameric Sm ring for the assembly of the ring complex on pre-snRNAs at the conserved Sm site [A(U)4-6G]. Gemin5, a WD40 protein component of the SMN complex, is responsible for recognizing pre-snRNAs. In addi...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.288340.116
更新日期：2016-11-01 00:00:00
abstract：:Homeotic genes are expressed in spatially precise patterns during Drosophila development to control segmental differentiation. The Sex combs reduced (Scr) gene of the Antennapedia gene complex is involved in the determination of the labial and prothoracic segments of the embryo. To study both the wild-type pattern of ...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.1.7.716
更新日期：1987-09-01 00:00:00
abstract：:SNF2 chromatin-remodeling ATPases play an important role in ensuring proper development in higher eukaryotes by controlling accessibility of cis-regulatory DNA regions to transcription factors and to the transcriptional machinery. However, the biological targets controlled by these ATPases are largely unknown. Using g...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.1276305
更新日期：2005-04-15 00:00:00
abstract：:Cytosolic RNA/DNA sensing elicits primary defense against viral pathogens. Interferon regulatory factor 3 (IRF3), a key signal mediator/transcriptional factor of the antiviral-sensing pathway, is indispensible for interferon production and antiviral defense. However, how the status of IRF3 activation is controlled rem...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.277533.116
更新日期：2016-05-01 00:00:00
