Abstract：
:Discrete sequence elements known as exonic splicing enhancers (ESEs) have been shown to influence both the efficiency of splicing and the profile of mature mRNAs in multicellular eukaryotes. While the existence of ESEs has not been demonstrated previously in unicellular eukaryotes, the factors known to recognize these elements and mediate their communication with the core splicing machinery are conserved and essential in the fission yeast Schizosaccharomyces pombe. Here, we provide evidence that ESE function is conserved through evolution by demonstrating that three exonic splicing enhancers derived from vertebrates (chicken ASLV, mouse IgM, and human cTNT) promote splicing of two distinct S. pombe pre-messenger RNAs (pre-mRNAs). Second, as in extracts from mammalian cells, ESE function in S. pombe is compromised by mutations and increased distance from the 3'-splice site. Third, three-hybrid analyses indicate that the essential SR (serine/arginine-rich) protein Srp2p, but not the dispensable Srp1p, binds specifically to both native and heterologous purine-rich elements; thus, Srp2p is the likely mediator of ESE function in fission yeast. Finally, we have identified five natural purine-rich elements from S. pombe that promote splicing of our reporter pre-mRNAs. Taken together, these results provide strong evidence that the genesis of ESE-mediated splicing occurred early in eukaryotic evolution.
journal_name
Genes Devjournal_title
Genes & developmentauthors
Webb CJ,Romfo CM,van Heeckeren WJ,Wise JAdoi
10.1101/gad.1265905subject
Has Abstractpub_date
2005-01-15 00:00:00pages
242-54issue
2eissn
0890-9369issn
1549-5477pii
gad.1265905journal_volume
19pub_type
杂志文章abstract：:In budding yeast, a switch between the mutually exclusive pathways of cell cycle progression and conjugation is controlled at Start in late G1 phase. Mating pheromones promote conjugation by arresting cells in G1 phase before Start. Pheromone-induced cell cycle arrest requires a functional FAR1 gene. We have found tha...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.7.5.833
更新日期：1993-05-01 00:00:00
abstract：:Treatment with retinoic acid (RA) is known to produce complex teratogenic effects in vertebrates, and its presence in the developing embryo as an endogenous substance has led to the suggestion that RA might be a natural morphogenetic agent. Although our understanding of the molecular mechanism of RA action has improve...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.5.8.1333
更新日期：1991-08-01 00:00:00
abstract：:Viable aristaless (al) mutations of Drosophila affect pattern elements at both ends of the proximodistal axis in a subset of adult appendages. The al gene has been cloned and identified by P-element-mediated germ-line transformation with a genomic DNA fragment, which rescues a lethal mutation of al as well as aspects ...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.7.1.114
更新日期：1993-01-01 00:00:00
abstract：:The ability to maintain and expand the pool of adipocytes in adults is integral to the regulation of energy balance, tissue/stem cell homeostasis, and disease pathogenesis. For decades, our knowledge of adipocyte precursors has relied on cellular models. The identity of native adipocyte precursors has remained unclear...
journal_title：Genes & development
pub_type： 杂志文章,评审
doi：10.1101/gad.293704.116
更新日期：2017-01-15 00:00:00
abstract：:Genetic and cytologic data from Saccharomyces cerevisiae and mammals implicate the Mre11 complex, consisting of Mre11, Rad50, and Nbs1, as a sensor of DNA damage, and indicate that the complex influences the activity of ataxia-telangiectasia mutated (ATM) in the DNA damage response. Rad50(S/S) mice exhibit precipitous...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.1373705
更新日期：2005-12-15 00:00:00
abstract：:Previous studies have shown that differentiation of 3T3-L1 preadipocytes leads to the transcriptional activation of a group of adipose-specific genes. As an approach to defining the mechanism responsible for activating the expression of these genes, we investigated the binding of nuclear factors to the promoters of tw...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.3.9.1323
更新日期：1989-09-01 00:00:00
abstract：:In Saccharomyces cerevisiae, the genes encoding the HO endonuclease, G1-specific cyclins CLN1 and CLN2, as well as most proteins involved in DNA synthesis, are periodically transcribed with maximal levels reached in late G1. For HO and the DNA replication genes, cell cycle stage-specific expression has been shown to b...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.6.4.557
更新日期：1992-04-01 00:00:00
abstract：:AMP-activated protein kinase (AMPK) is a sensor of energy status that maintains cellular energy homeostasis. It arose very early during eukaryotic evolution, and its ancestral role may have been in the response to starvation. Recent work shows that the kinase is activated by increases not only in AMP, but also in ADP....
journal_title：Genes & development
pub_type： 杂志文章,评审
doi：10.1101/gad.17420111
更新日期：2011-09-15 00:00:00
abstract：:Plant water homeostasis is maintained by the phytohormone abscisic acid (ABA), which triggers stomatal pore closure in response to drought stress. We identified the Arabidopsis small guanosine triphosphatase (GTPase) protein AtRac1 as a central component in the ABA-mediated stomatal closure process. ABA treatment indu...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.900401
更新日期：2001-07-15 00:00:00
abstract：:The Agrobacterium Ti plasmid (T-DNA) 6b proteins interact with many different host proteins implicated in plant cell proliferation. Here, we show that Arabidopsis plants overexpressing 6b display microRNA (miRNA) deficiency by directly targeting SERRATE and AGO1 via a specific loop fragment (residues 40-55). In additi...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.1985511
更新日期：2011-01-01 00:00:00
abstract：:Commitment to mitosis is regulated by a protein kinase complex called MPF. MPF is inhibited by Wee1-related kinases and activated by Cdc25 phosphatase. MPF activation further boosts Cdc25 and represses Wee1. This feedback control probably involves polo kinase. A dominant cut12.s11 mutation in the Schizosaccharomyces p...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.256003
更新日期：2003-06-15 00:00:00
abstract：:Mesoderm induction assays in Xenopus have implicated growth factors such as activin, Vg1, Xwnt-8, and noggin as important in directing the formation of dorsal mesoderm (Spemann's organizer). Because these growth factors are structurally very different, they presumably act through distinct cell surface receptors that i...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.9.24.3038
更新日期：1995-12-15 00:00:00
abstract：:Alternative polyadenylation (APA) is widespread in neuronal development and activity-mediated neural plasticity. However, the underlying molecular mechanisms are largely unknown. We used systematic genetic studies and genome-wide surveys of the transcriptional landscape to identify a context-dependent regulatory pathw...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.266650.115
更新日期：2015-11-15 00:00:00
abstract：:In the absence of growth signals, cells exit the cell cycle and enter into G0 or quiescence. Alternatively, cells enter senescence in response to inappropriate growth signals such as oncogene expression. The molecular mechanisms required for cell cycle exit into quiescence or senescence are poorly understood. The DREA...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.2034211
更新日期：2011-04-15 00:00:00
abstract：:The control of translation and mRNA degradation is an important part of the regulation of gene expression. It is now clear that small RNA molecules are common and effective modulators of gene expression in many eukaryotic cells. These small RNAs that control gene expression can be either endogenous or exogenous micro ...
journal_title：Genes & development
pub_type： 杂志文章,评审
doi：10.1101/gad.1399806
更新日期：2006-03-01 00:00:00
abstract：:Transcription factors represent one of the largest groups of proteins regulated by SUMO (small ubiquitin-like modifier) modification, and their sumoylation is usually associated with transcriptional repression. To investigate whether sumoylation plays a general role in regulating transcription in yeast, we determined ...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.1917910
更新日期：2010-06-15 00:00:00
abstract：:Numerous lines of study have suggested that the Hox genes, encoding putative transcription factors, are key genes in the establishment of the body plan of the mammalian embryo. To examine the role of Hoxa-5 (Hox1.3) gene during development, we have used targeted mutagenesis in embryonic stem cells to produce a strain ...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.7.11.2085
更新日期：1993-11-01 00:00:00
abstract：:Ectopic repression of retinoic acid (RA) receptor target genes by PML/RARA and PLZF/RARA fusion proteins through aberrant recruitment of nuclear corepressor complexes drives cellular transformation and acute promyelocytic leukemia (APL) development. In the case of PML/RARA, this repression can be reversed through trea...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.512009
更新日期：2009-05-15 00:00:00
abstract：:The Snail zinc-finger transcription factors trigger epithelial-mesenchymal transitions (EMTs), endowing epithelial cells with migratory and invasive properties during both embryonic development and tumor progression. During EMT, Snail provokes the loss of epithelial markers, as well as changes in cell shape and the ex...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.294104
更新日期：2004-05-15 00:00:00
abstract：:Chromosome replication occurs precisely once during the cell cycle of almost all eukaryotic cells, and is a highly complex process that is still understood relatively poorly. Two conserved kinases called Cdc7 (cell division cycle 7) and cyclin-dependent kinase (CDK) are required to establish replication forks during t...
journal_title：Genes & development
pub_type： 杂志文章,评审
doi：10.1101/gad.1933010
更新日期：2010-06-15 00:00:00
abstract：:The nuclear receptor peroxisome proliferator-activated receptor γ (PPARγ) is known to regulate lipid metabolism in many tissues, including macrophages. Here we report that peritoneal macrophage respiration is enhanced by rosiglitazone, an activating PPARγ ligand, in a PPARγ-dependent manner. Moreover, PPARγ is require...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.312355.118
更新日期：2018-08-01 00:00:00
abstract：:The td, nrdB, and sunY introns of bacteriophage T4 each contain a long open reading frame (ORF). These ORFs are preceded by functional T4 late promoters and, in the case of the nrdB intron ORF, a functional middle promoter. Expression of phage-encoded intron ORF-lacZ fusions indicates that these T4 genes are highly re...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.2.12b.1791
更新日期：1988-12-01 00:00:00
abstract：:Irradiation in the G1 phase of the cell cycle delays the onset of DNA synthesis and transiently inhibits the activation of replication origins in mammalian cells. It has been suggested that this inhibition is the result of the loss of torsional tension in the DNA after it has been damaged. Because irradiation causes D...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.8.5.554
更新日期：1994-03-01 00:00:00
abstract：:In yeast, galactose triggers a rapid GAL4-dependent induction of galactose/melibiose regulon (GAL/MEL) gene transcription, and glucose represses this activation. We discovered that alterations in the physical state of the GAL4 protein correlate with activation and repression of the GAL/MEL genes. Using Western immunob...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.3.8.1157
更新日期：1989-08-01 00:00:00
abstract：:Inspired by a zebrafish mutation, two recent studies by Creanga and colleagues (pp. 1312-1325) and Tukachinsky and colleagues have shed new light on the way in which lipidated Hedgehog proteins are secreted and released from expressing cells, suggesting a model for the sequential action of the Disp and Scube2 proteins...
journal_title：Genes & development
pub_type： 评论,杂志文章
doi：10.1101/gad.207126.112
更新日期：2012-11-15 00:00:00
abstract：:Post-translational processes are essential for the generation and dynamics of mammalian circadian rhythms. In particular, phosphorylation of the key circadian protein PER2 precisely controls the period and phase of circadian oscillations. However, the mechanisms underlying that control are poorly understood. Here, we ...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.512209
更新日期：2009-03-15 00:00:00
abstract：:Genome integrity is constantly monitored by sophisticated cellular networks, collectively termed the DNA damage response (DDR). A common feature of DDR proteins is their mobilization in response to genotoxic stress. Here, we outline how the development of various complementary methodologies has provided valuable insig...
journal_title：Genes & development
pub_type： 杂志文章,评审
doi：10.1101/gad.2021311
更新日期：2011-03-01 00:00:00
abstract：:The serine/threonine kinase Akt/PKB is a major downstream effector of growth factor-mediated cell survival. Activated Akt, like Bcl-2 and Bcl-xL, prevents closure of a PT pore component, the voltage-dependent anion channel (VDAC); intracellular acidification; mitochondrial hyperpolarization; and the decline in oxidati...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.889901
更新日期：2001-06-01 00:00:00
abstract：:Previous genetic mosaic studies established that expression of the Drosophila bride of sevenless (boss) gene is required in photoreceptor neuron R8 for the development of photoreceptor neuron R7. This led to the proposal that boss encodes or regulates an R7-specific inductive cue. We have identified the boss gene base...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.4.11.1835
更新日期：1990-11-01 00:00:00
abstract：:Translation initiation factor 2 (eIF2) bound to GTP transfers the initiator methionyl tRNA to the 40S ribosomal subunit. The eIF5 stimulates GTP hydrolysis by the eIF2/GTP/Met-tRNA(i)(Met) ternary complex on base-pairing between Met-tRNA(i)(Met) and the start codon. The eIF2, eIF5, and eIF1 all have been implicated in...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.831800
更新日期：2000-10-01 00:00:00
