Abstract:
:The Agrobacterium Ti plasmid (T-DNA) 6b proteins interact with many different host proteins implicated in plant cell proliferation. Here, we show that Arabidopsis plants overexpressing 6b display microRNA (miRNA) deficiency by directly targeting SERRATE and AGO1 via a specific loop fragment (residues 40-55). In addition, we report the crystal structures of Agrobacterium tumefaciens AK6b at 2.1 Å, Agrobacterium vitis AB6b at 1.65 Å, and Arabidopsis ADP ribosylation factor (ARF) at 1.8 Å. The 6b structure adopts an ADP-ribosylating toxin fold closely related to cholera toxin. In vitro ADP ribosylation analysis demonstrates that 6b represents a new toxin family, with Tyr 66, Thr 93, and Tyr 153 as the ADP ribosylation catalytic residues in the presence of Arabidopsis ARF and GTP. Our work provides molecular insights, suggesting that 6b regulates plant cell growth by the disturbance of the miRNA pathway through its ADP ribosylation activity.
journal_name
Genes Devjournal_title
Genes & developmentauthors
Wang M,Soyano T,Machida S,Yang JY,Jung C,Chua NH,Yuan YAdoi
10.1101/gad.1985511subject
Has Abstractpub_date
2011-01-01 00:00:00pages
64-76issue
1eissn
0890-9369issn
1549-5477pii
gad.1985511journal_volume
25pub_type
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journal_title:Genes & development
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