Uniform gene expression in embryos is achieved by temporal averaging of transcription noise.

abstract：:Genome instability is a recurring feature of tumorigenesis. Mutation in MLL2, encoding a histone methyltransferase, is a driver in numerous different cancer types, but the mechanism is unclear. Here, we present evidence that MLL2 mutation results in genome instability. Mouse cells in which MLL2 gene deletion can be in...

journal_title：Genes & development

authors： Kantidakis T,Saponaro M,Mitter R,Horswell S,Kranz A,Boeing S,Aygün O,Kelly GP,Matthews N,Stewart A,Stewart AF,Svejstrup JQ

更新日期：2016-02-15 00:00:00

abstract：:An insulin receptor-like signaling pathway regulates Caenorhabditis elegans metabolism, development, and longevity. Inactivation of the insulin receptor homolog DAF-2, the AGE-1 PI3K, or the AKT-1 and AKT-2 kinases causes a developmental arrest at the dauer stage. A null mutation in the daf-16 Fork head transcription ...

journal_title：Genes & development

authors： Paradis S,Ailion M,Toker A,Thomas JH,Ruvkun G

更新日期：1999-06-01 00:00:00

abstract：:As in many polarized cells, spindle alignment in yeast is essential and cell cycle regulated. A key step that governs spindle alignment is the selective binding of the Kar9 protein to only one of the two spindle pole bodies (SPBs). It has been suggested that cyclin-dependent kinase Cdc28, in complex with cyclin Clb4, ...

journal_title：Genes & development

authors： Maekawa H,Schiebel E

更新日期：2004-07-15 00:00:00

abstract：:Stem cells have been identified as a source of virtually all highly differentiated cells that are replenished during the lifetime of an animal. The critical balance between stem and differentiated cell populations is crucial for the long-term maintenance of functional tissue types. Stem cells maintain this balance by ...

journal_title：Genes & development

authors： Martinez-Agosto JA,Mikkola HK,Hartenstein V,Banerjee U

更新日期：2007-12-01 00:00:00

abstract：:Integrin-mediated cell-matrix interactions are essential for development, tissue homeostasis, and repair. Upon ligand binding, integrins are recruited into focal adhesions (FAs). Integrin-linked kinase (ILK) is an FA component that interacts with the cytoplasmic domains of integrins, recruits adaptor proteins that lin...

journal_title：Genes & development

authors： Sakai T,Li S,Docheva D,Grashoff C,Sakai K,Kostka G,Braun A,Pfeifer A,Yurchenco PD,Fässler R

更新日期：2003-04-01 00:00:00

abstract：:Chromosome replication occurs precisely once during the cell cycle of almost all eukaryotic cells, and is a highly complex process that is still understood relatively poorly. Two conserved kinases called Cdc7 (cell division cycle 7) and cyclin-dependent kinase (CDK) are required to establish replication forks during t...

journal_title：Genes & development

authors： Labib K

更新日期：2010-06-15 00:00:00

abstract：:We have shown that Wnt signals are necessary and sufficient for neural crest cells to adopt pigment cell fates. nacre, a zebrafish homolog of MITF, is required for pigment cell differentiation. We isolated a promoter region of nacre that contains Tcf/Lef binding sites, which can mediate Wnt responsiveness. This promot...

journal_title：Genes & development

authors： Dorsky RI,Raible DW,Moon RT

更新日期：2000-01-15 00:00:00

abstract：:Heparan sulfate proteoglycans have been implicated in the presentation of a number of secreted signaling molecules to their signal-transducing receptors. We have characterized a gene trap mutation in the gene encoding a heparan sulfate biosynthetic enzyme, heparan sulfate 2-sulfotransferase (HS2ST). Transgenic mice we...

journal_title：Genes & development

authors： Bullock SL,Fletcher JM,Beddington RS,Wilson VA

更新日期：1998-06-15 00:00:00

abstract：:The initial steps of double-stranded break (DSB) repair by homologous recombination mediated by the 5'-3' exonuclease/annealing protein pairs, RecE/RecT and Redalpha/Redbeta, were analyzed. Recombination was RecA-independent and required the expression of both components of an orthologous pair, even when the need for ...

journal_title：Genes & development

authors： Muyrers JP,Zhang Y,Buchholz F,Stewart AF

更新日期：2000-08-01 00:00:00

abstract：:Fibro-adipogenic progenitors (FAPs) reside in the muscle, where they facilitate myofiber regeneration. Under normal conditions, FAPs lack myogenic potential and thus do not directly contribute to regenerated myofibers. Surprisingly, Saccone and colleagues (pp. 841-857) demonstrated that the dystrophic muscle environme...

journal_title：Genes & development

authors： Faralli H,Dilworth FJ

更新日期：2014-04-15 00:00:00

abstract：:We have developed a simple, effective genetic screen for mutant alleles of eukaryotic DNA topoisomerase I that manifest severely depressed or complete loss of enzymatic function. The screen is based on the extreme toxicity of vaccinia topoisomerase expression in the Escherichia coli lysogen strain BL21(DE3) and is not...

journal_title：Genes & development

authors： Morham SG,Shuman S

更新日期：1990-04-01 00:00:00

abstract：:The circadian rhythm of pineal melatonin requires the nocturnal increment of serotonin N-acetyltransferase (arylalkylamine N-acetyltransferase [AANAT]) protein. To date, only limited information is available in the critical issue of how AANAT protein expression is up-regulated exclusively at night regardless of its sp...

journal_title：Genes & development

authors： Kim TD,Woo KC,Cho S,Ha DC,Jang SK,Kim KT

更新日期：2007-04-01 00:00:00

abstract：:Cdc42p, a Rho-related GTP-binding protein, regulates cytoskeletal polarization and rearrangements in eukaryotic cells, but the effectors mediating this control remain unknown. Through the use of the complete yeast genomic sequence, we have identified two novel Cdc42p targets, Gic1p and Gic2p, which contain consensus C...

journal_title：Genes & development

authors： Brown JL,Jaquenoud M,Gulli MP,Chant J,Peter M

更新日期：1997-11-15 00:00:00

abstract：:The dihydrofolate reductase (DHFR) and 2BE2121 genes in the Chinese hamster are convergently transcribed in late G1 and ea ly S phase, and bracket an early-firing origin of replication that consists of a 55-kb zone of potential initiation sites. To test whether transcription through the DHFR gene is required to activa...

journal_title：Genes & development

authors： Saha S,Shan Y,Mesner LD,Hamlin JL

更新日期：2004-02-15 00:00:00

abstract：:Nuclear export factor 1 (NXF1) exports mRNA to the cytoplasm after recruitment to mRNA by specific adaptor proteins. How and why cells use numerous different export adaptors is poorly understood. Here we critically evaluate members of the SR protein family (SRSF1-7) for their potential to act as NXF1 adaptors that cou...

journal_title：Genes & development

authors： Müller-McNicoll M,Botti V,de Jesus Domingues AM,Brandl H,Schwich OD,Steiner MC,Curk T,Poser I,Zarnack K,Neugebauer KM

更新日期：2016-03-01 00:00:00

abstract：:The serine/threonine kinase Akt/PKB is a major downstream effector of growth factor-mediated cell survival. Activated Akt, like Bcl-2 and Bcl-xL, prevents closure of a PT pore component, the voltage-dependent anion channel (VDAC); intracellular acidification; mitochondrial hyperpolarization; and the decline in oxidati...

journal_title：Genes & development

authors： Gottlob K,Majewski N,Kennedy S,Kandel E,Robey RB,Hay N

更新日期：2001-06-01 00:00:00

abstract：:Histone post-translational modifications regulate chromatin structure and function largely through interactions with effector proteins that often contain multiple histone-binding domains. While significant progress has been made characterizing individual effector domains, the role of paired domains and how they functi...

journal_title：Genes & development

authors： Rothbart SB,Dickson BM,Ong MS,Krajewski K,Houliston S,Kireev DB,Arrowsmith CH,Strahl BD

更新日期：2013-06-01 00:00:00

abstract：:A novel histone methyltransferase, termed Set9, was isolated from human cells. Set9 contains a SET domain, but lacks the pre- and post-SET domains. Set9 methylates specifically lysine 4 (K4) of histone H3 (H3-K4) and potentiates transcription activation. The histone H3 tail interacts specifically with the histone deac...

journal_title：Genes & development

authors： Nishioka K,Chuikov S,Sarma K,Erdjument-Bromage H,Allis CD,Tempst P,Reinberg D

更新日期：2002-02-15 00:00:00

abstract：:Sox2 is expressed in basal epithelial cells of the tongue, with high levels in taste bud placodes, fungiform papillae, and mature taste cells, and low levels in filiform papillae. High Sox2 expression appears to lie downstream from canonical Wnt signaling. In hypomorphic Sox2(EGFP/LP) embryos, placodes form but no mat...

journal_title：Genes & development

authors： Okubo T,Pevny LH,Hogan BL

更新日期：2006-10-01 00:00:00

abstract：:The p53 tumor suppressor is often disrupted in human cancers by the acquisition of missense mutations. We generated mice with a missense mutation at codon 172 that mimics the p53R175H hot spot mutation in human cancer. p53 homozygous mutant mice have unstable mutant p53 in normal cells and stabilize mutant p53 in some...

journal_title：Genes & development

authors： Terzian T,Suh YA,Iwakuma T,Post SM,Neumann M,Lang GA,Van Pelt CS,Lozano G

更新日期：2008-05-15 00:00:00

abstract：:Oncogene-induced senescence (OIS) is a potent tumor suppressor mechanism. To identify senescence regulators relevant to cancer, we screened an shRNA library targeting genes deleted in hepatocellular carcinoma (HCC). Here, we describe how knockdown of the SWI/SNF component ARID1B prevents OIS and cooperates with RAS to...

journal_title：Genes & development

authors： Tordella L,Khan S,Hohmeyer A,Banito A,Klotz S,Raguz S,Martin N,Dhamarlingam G,Carroll T,González Meljem JM,Deswal S,Martínez-Barbera JP,García-Escudero R,Zuber J,Zender L,Gil J

更新日期：2016-10-01 00:00:00

abstract：:The RAG-1 protein plays an essential role in V(D)j recombination, but its exact function has not yet been defined. Here we report that a particular mutation in RAG-1 affects recombination by altering the specificity of target sequence usage. Recombination mediated by wild-type RAG-1 is tolerant of a wide range of codi...

journal_title：Genes & development

authors： Sadofsky MJ,Hesse JE,van Gent DC,Gellert M

更新日期：1995-09-01 00:00:00

abstract：:Cyclin B3 has been conserved during higher eukaryote evolution as evidenced by its identification in chicken, nematodes, and insects. We demonstrate that Cyclin B3 is present in addition to Cyclins A and B in mitotically proliferating cells and not detectable in endoreduplicating tissues of Drosophila embryos. Cyclin ...

journal_title：Genes & development

authors： Jacobs HW,Knoblich JA,Lehner CF

更新日期：1998-12-01 00:00:00

abstract：:Erasure and subsequent reinstatement of DNA methylation in the germline, especially at imprinted CpG islands (CGIs), is crucial to embryogenesis in mammals. The mechanisms underlying DNA methylation establishment remain poorly understood, but a number of post-translational modifications of histones are implicated in a...

journal_title：Genes & development

authors： Stewart KR,Veselovska L,Kim J,Huang J,Saadeh H,Tomizawa S,Smallwood SA,Chen T,Kelsey G

更新日期：2015-12-01 00:00:00

abstract：:AMP-activated protein kinase (AMPK) is a sensor of energy status that maintains cellular energy homeostasis. It arose very early during eukaryotic evolution, and its ancestral role may have been in the response to starvation. Recent work shows that the kinase is activated by increases not only in AMP, but also in ADP....

journal_title：Genes & development

authors： Hardie DG

更新日期：2011-09-15 00:00:00

abstract：:The assembly of activated RNA polymerase II (pol II) transcription complexes has been investigated by assaying whether pre-assembly of intermediate complexes reduces the extended time required for start-site melting. The results show that a closed complex requiring factors IIA, IID, and the acidic activator GAL4-AH fo...

journal_title：Genes & development

authors： Wang W,Gralla JD,Carey M

更新日期：1992-09-01 00:00:00

abstract：:Start sites of DNA replication are marked by the origin recognition complex (ORC), which coordinates Mcm2-7 helicase loading to form the prereplicative complex (pre-RC). Although pre-RC assembly is well characterized in vitro, the process is poorly understood within the local chromatin environment surrounding replicat...

journal_title：Genes & development

authors： Belsky JA,MacAlpine HK,Lubelsky Y,Hartemink AJ,MacAlpine DM

更新日期：2015-01-15 00:00:00

abstract：:Adenomatous polyposis coli (APC) regulates the activity of β-catenin, an integral component of Wnt signaling. However, the selective role of the APC-β-catenin pathway in cerebral cortical development is unknown. Here we genetically dissected the relative contributions of APC-regulated β-catenin signaling in cortical p...

journal_title：Genes & development

authors： Nakagawa N,Li J,Yabuno-Nakagawa K,Eom TY,Cowles M,Mapp T,Taylor R,Anton ES

更新日期：2017-08-15 00:00:00

abstract：:Dendrite arborization patterns are critical determinants of neuronal function. To explore the basis of transcriptional regulation in dendrite pattern formation, we used RNA interference (RNAi) to screen 730 transcriptional regulators and identified 78 genes involved in patterning the stereotyped dendritic arbors of cl...

journal_title：Genes & development

authors： Parrish JZ,Kim MD,Jan LY,Jan YN

更新日期：2006-04-01 00:00:00

abstract：:By studying neuroectoderm formation in the absence of mesoderm and mesectoderm in mutants of the zygotic genes snail and twist, we have found that the number of neuroblasts is not reduced in these mutants, suggesting that mesoderm and mesectoderm are not essential for the initiation of neural development. The position...

journal_title：Genes & development

authors： Rao Y,Vaessin H,Jan LY,Jan YN

更新日期：1991-09-01 00:00:00