Fission yeast genes that confer resistance to staurosporine encode an AP-1-like transcription factor and a protein kinase related to the mammalian ERK1/MAP2 and budding yeast FUS3 and KSS1 kinases.


:Staurosporine, a potent inhibitor of protein kinase C, arrests fission yeast cell elongation specifically at a stage immediately after cell division. We isolated two genes, which, when carried on multicopy plasmids, confer drug resistance in fission yeast. One, spk1+, encodes a protein kinase highly similar (54% identity) to those encoded by the mammalian ERK1/MAP2 kinase and the budding yeast KSS1 and FUS3 genes. It is not essential for vegetative growth of Schizosaccharomyces pombe cells but is required for conjugation. The spk1+ gene product is a 45-kD protein enriched in the nucleus, and its level increases 10-fold after addition of staurosporine. The other gene pap1+ encodes an AP-1-like transcription factor that contains a region rich in basic amino acids followed by a "leucine zipper" motif. The pap1+ gene is required for spk1(+)-conferred staurosporine resistance. These two genes appear to function as a part of the fission yeast growth control pathway.


Genes Dev


Genes & development


Toda T,Shimanuki M,Yanagida M




Has Abstract


1991-01-01 00:00:00












  • Functional domains of the yeast transcription/replication factor MCM1.

    abstract::MCM1 is an essential yeast DNA-binding protein that affects both minichromosome maintenance, in a manner suggesting that it has DNA replication initiation function, and gene expression. It activates alpha-specific genes together with MAT alpha 1, and represses a-specific genes together with MAT alpha 2. Alone, MCM1 ca...

    journal_title:Genes & development

    pub_type: 杂志文章


    authors: Christ C,Tye BK

    更新日期:1991-05-01 00:00:00

  • Runx1 prevents wasting, myofibrillar disorganization, and autophagy of skeletal muscle.

    abstract::Disruptions in the use of skeletal muscle lead to muscle atrophy. After short periods of disuse, muscle atrophy is reversible, and even after prolonged periods of inactivity, myofiber degeneration is uncommon. The pathways that regulate atrophy, initiated either by peripheral nerve damage, immobilization, aging, catab...

    journal_title:Genes & development

    pub_type: 杂志文章


    authors: Wang X,Blagden C,Fan J,Nowak SJ,Taniuchi I,Littman DR,Burden SJ

    更新日期:2005-07-15 00:00:00

  • PPARγ is a nexus controlling alternative activation of macrophages via glutamine metabolism.

    abstract::The nuclear receptor peroxisome proliferator-activated receptor γ (PPARγ) is known to regulate lipid metabolism in many tissues, including macrophages. Here we report that peritoneal macrophage respiration is enhanced by rosiglitazone, an activating PPARγ ligand, in a PPARγ-dependent manner. Moreover, PPARγ is require...

    journal_title:Genes & development

    pub_type: 杂志文章


    authors: Nelson VL,Nguyen HCB,Garcìa-Cañaveras JC,Briggs ER,Ho WY,DiSpirito JR,Marinis JM,Hill DA,Lazar MA

    更新日期:2018-08-01 00:00:00

  • N-myc alters the fate of preneoplastic cells in a mouse model of medulloblastoma.

    abstract::Studying the early stages of cancer can provide important insight into the molecular basis of the disease. We identified a preneoplastic stage in the patched (ptc) mutant mouse, a model for the brain tumor medulloblastoma. Preneoplastic cells (PNCs) are found in most ptc mutants during early adulthood, but only 15% of...

    journal_title:Genes & development

    pub_type: 杂志文章


    authors: Kessler JD,Hasegawa H,Brun SN,Emmenegger BA,Yang ZJ,Dutton JW,Wang F,Wechsler-Reya RJ

    更新日期:2009-01-15 00:00:00

  • pRb and condensin--local control of global chromosome structure.

    abstract::Rb mutants exhibit aneuploidy and aberrant chromosome structure during mitosis. In this issue of Genes & Development, a new paper from Longworth and colleagues (1011-1024) describes both physical and functional interactions between Drosophila Rbf1 and the dCAP-D3 subunit of condensin II. This work directly implicates ...

    journal_title:Genes & development

    pub_type: 评论,杂志文章


    authors: Lavoie BD

    更新日期:2008-04-15 00:00:00

  • TBP, Mot1, and NC2 establish a regulatory circuit that controls DPE-dependent versus TATA-dependent transcription.

    abstract::The RNA polymerase II core promoter is a structurally and functionally diverse transcriptional module. RNAi depletion and overexpression experiments revealed a genetic circuit that controls the balance of transcription from two core promoter motifs, the TATA box and the downstream core promoter element (DPE). In this ...

    journal_title:Genes & development

    pub_type: 杂志文章


    authors: Hsu JY,Juven-Gershon T,Marr MT 2nd,Wright KJ,Tjian R,Kadonaga JT

    更新日期:2008-09-01 00:00:00

  • Pax6 and SOX2 form a co-DNA-binding partner complex that regulates initiation of lens development.

    abstract::Pax6 is a key transcription factor in eye development, particularly in lens development, but its molecular action has not been clarified. We demonstrate that Pax6 initiates lens development by forming a molecular complex with SOX2 on the lens-specific enhancer elements, e.g., the delta-crystallin minimal enhancer DC5....

    journal_title:Genes & development

    pub_type: 杂志文章


    authors: Kamachi Y,Uchikawa M,Tanouchi A,Sekido R,Kondoh H

    更新日期:2001-05-15 00:00:00

  • An inducible change in Fox-1/A2BP1 splicing modulates the alternative splicing of downstream neuronal target exons.

    abstract::Neuronal depolarization and CaM kinase IV signaling alter the splicing of multiple exons in transcripts for ion channels, neurotransmitter receptors, and other synaptic proteins. These splicing changes are mediated in part by special CaM kinase-responsive RNA elements, within or adjacent to exons that are repressed in...

    journal_title:Genes & development

    pub_type: 杂志文章


    authors: Lee JA,Tang ZZ,Black DL

    更新日期:2009-10-01 00:00:00

  • De- and repolarization mechanism of flagellar morphogenesis during a bacterial cell cycle.

    abstract::Eukaryotic morphogenesis is seeded with the establishment and subsequent amplification of polarity cues at key times during the cell cycle, often using (cyclic) nucleotide signals. We discovered that flagellum de- and repolarization in the model prokaryote Caulobacter crescentus is precisely orchestrated through at le...

    journal_title:Genes & development

    pub_type: 杂志文章


    authors: Davis NJ,Cohen Y,Sanselicio S,Fumeaux C,Ozaki S,Luciano J,Guerrero-Ferreira RC,Wright ER,Jenal U,Viollier PH

    更新日期:2013-09-15 00:00:00

  • The hematopoietic stem cell and its niche: a comparative view.

    abstract::Stem cells have been identified as a source of virtually all highly differentiated cells that are replenished during the lifetime of an animal. The critical balance between stem and differentiated cell populations is crucial for the long-term maintenance of functional tissue types. Stem cells maintain this balance by ...

    journal_title:Genes & development

    pub_type: 杂志文章,评审


    authors: Martinez-Agosto JA,Mikkola HK,Hartenstein V,Banerjee U

    更新日期:2007-12-01 00:00:00

  • Changes in histone gene dosage alter transcription in yeast.

    abstract::Chromatin structure is believed to be important for a number of cellular processes, including transcription. However, the role of nucleosomes in transcription is not well understood. We have identified the yeast histone locus HTB1-HTB1, encoding histones H2A and H2B, as a suppressor of solo delta insertion mutations t...

    journal_title:Genes & development

    pub_type: 杂志文章


    authors: Clark-Adams CD,Norris D,Osley MA,Fassler JS,Winston F

    更新日期:1988-02-01 00:00:00

  • Direct induction of G1-specific transcripts following reactivation of the Cdc28 kinase in the absence of de novo protein synthesis.

    abstract::In Saccharomyces cerevisiae, the genes encoding the HO endonuclease, G1-specific cyclins CLN1 and CLN2, as well as most proteins involved in DNA synthesis, are periodically transcribed with maximal levels reached in late G1. For HO and the DNA replication genes, cell cycle stage-specific expression has been shown to b...

    journal_title:Genes & development

    pub_type: 杂志文章


    authors: Marini NJ,Reed SI

    更新日期:1992-04-01 00:00:00

  • Smad proteins act in combination with synergistic and antagonistic regulators to target Dpp responses to the Drosophila mesoderm.

    abstract::Dorsal mesoderm induction in arthropods and ventral mesoderm induction in vertebrates are closely related processes that involve signals of the BMP family. In Drosophila, induction of visceral mesoderm, dorsal muscles, and the heart by Dpp is, at least in part, effected through the transcriptional activation and funct...

    journal_title:Genes & development

    pub_type: 杂志文章


    authors: Xu X,Yin Z,Hudson JB,Ferguson EL,Frasch M

    更新日期:1998-08-01 00:00:00

  • Double-bromo and extraterminal (BET) domain proteins regulate dendrite morphology and mechanosensory function.

    abstract::A complex array of genetic factors regulates neuronal dendrite morphology. Epigenetic regulation of gene expression represents a plausible mechanism to control pathways responsible for specific dendritic arbor shapes. By studying the Drosophila dendritic arborization (da) neurons, we discovered a role of the double-br...

    journal_title:Genes & development

    pub_type: 杂志文章


    authors: Bagley JA,Yan Z,Zhang W,Wildonger J,Jan LY,Jan YN

    更新日期:2014-09-01 00:00:00

  • Infrequently transcribed long genes depend on the Set2/Rpd3S pathway for accurate transcription.

    abstract::The presence of Set2-mediated methylation of H3K36 (K36me) correlates with transcription frequency throughout the yeast genome. K36me targets the Rpd3S complex to deacetylate transcribed regions and suppress cryptic transcription initiation at certain genes. Here, using a genome-wide approach, we report that the Set2-...

    journal_title:Genes & development

    pub_type: 杂志文章


    authors: Li B,Gogol M,Carey M,Pattenden SG,Seidel C,Workman JL

    更新日期:2007-06-01 00:00:00

  • Set9, a novel histone H3 methyltransferase that facilitates transcription by precluding histone tail modifications required for heterochromatin formation.

    abstract::A novel histone methyltransferase, termed Set9, was isolated from human cells. Set9 contains a SET domain, but lacks the pre- and post-SET domains. Set9 methylates specifically lysine 4 (K4) of histone H3 (H3-K4) and potentiates transcription activation. The histone H3 tail interacts specifically with the histone deac...

    journal_title:Genes & development

    pub_type: 杂志文章


    authors: Nishioka K,Chuikov S,Sarma K,Erdjument-Bromage H,Allis CD,Tempst P,Reinberg D

    更新日期:2002-02-15 00:00:00

  • Brachyury downstream notochord differentiation in the ascidian embryo.

    abstract::The ascidian tadpole represents the most simplified chordate body plan. It contains a notochord composed of just 40 cells, but as in vertebrates Brachyury is essential for notochord differentiation. Here, we show that the misexpression of the Brachyury gene (Ci-Bra) of Ciona intestinalis is sufficient to transform end...

    journal_title:Genes & development

    pub_type: 杂志文章


    authors: Takahashi H,Hotta K,Erives A,Di Gregorio A,Zeller RW,Levine M,Satoh N

    更新日期:1999-06-15 00:00:00

  • The neuron-specific RNA-binding protein ELAV regulates neuroglian alternative splicing in neurons and binds directly to its pre-mRNA.

    abstract::Drosophila melanogaster neural-specific protein, ELAV, has been shown to regulate the neural-specific splicing of three genes: neuroglian (nrg), erect wing, and armadillo. Alternative splicing of the nrg transcript involves alternative inclusion of a 3'-terminal exon. Here, using a minigene reporter, we show that the ...

    journal_title:Genes & development

    pub_type: 杂志文章


    authors: Lisbin MJ,Qiu J,White K

    更新日期:2001-10-01 00:00:00

  • A PTIP-PA1 subcomplex promotes transcription for IgH class switching independently from the associated MLL3/MLL4 methyltransferase complex.

    abstract::Class switch recombination (CSR) diversifies antibodies for productive immune responses while maintaining stability of the B-cell genome. Transcription at the immunoglobulin heavy chain (Igh) locus targets CSR-associated DNA damage and is promoted by the BRCT domain-containing PTIP (Pax transactivation domain-interact...

    journal_title:Genes & development

    pub_type: 杂志文章


    authors: Starnes LM,Su D,Pikkupeura LM,Weinert BT,Santos MA,Mund A,Soria R,Cho YW,Pozdnyakova I,Kubec Højfeldt M,Vala A,Yang W,López-Méndez B,Lee JE,Peng W,Yuan J,Ge K,Montoya G,Nussenzweig A,Choudhary C,Daniel JA

    更新日期:2016-01-15 00:00:00

  • A novel, activin-inducible, blastopore lip-specific gene of Xenopus laevis contains a fork head DNA-binding domain.

    abstract::The organizer region, or dorsal blastopore lip, plays a central role in the initiation of gastrulation and the formation of the body axis during Xenopus development. A similar process can also be induced in ectodermal explants by activin or by injection of activin mRNA into embryos. We have searched early embryo-speci...

    journal_title:Genes & development

    pub_type: 杂志文章


    authors: Dirksen ML,Jamrich M

    更新日期:1992-04-01 00:00:00

  • Phage N4 RNA polymerase II recruitment to DNA by a single-stranded DNA-binding protein.

    abstract::Transcription of bacteriophage N4 middle genes is carried out by a phage-coded, heterodimeric RNA polymerase (N4 RNAPII), which belongs to the family of T7-like RNA polymerases. In contrast to phage T7-RNAP, N4 RNAPII displays no activity on double-stranded templates and low activity on single-stranded templates. In v...

    journal_title:Genes & development

    pub_type: 杂志文章


    authors: Carter RH,Demidenko AA,Hattingh-Willis S,Rothman-Denes LB

    更新日期:2003-09-15 00:00:00

  • Genomic imprinting of Xist by maternal H3K27me3.

    abstract::Maternal imprinting at the Xist gene is essential to achieve paternal allele-specific imprinted X-chromosome inactivation (XCI) in female mammals. However, the mechanism underlying Xist imprinting is unclear. Here we show that the Xist locus is coated with a broad H3K27me3 domain that is established during oocyte grow...

    journal_title:Genes & development

    pub_type: 杂志文章


    authors: Inoue A,Jiang L,Lu F,Zhang Y

    更新日期:2017-10-01 00:00:00

  • Differential Tks5 isoform expression contributes to metastatic invasion of lung adenocarcinoma.

    abstract::Metastasis accounts for the vast majority of cancer-related deaths, yet the molecular mechanisms that drive metastatic spread remain poorly understood. Here we report that Tks5, which has been linked to the formation of proteolytic cellular protrusions known as invadopodia, undergoes an isoform switch during metastati...

    journal_title:Genes & development

    pub_type: 杂志文章


    authors: Li CM,Chen G,Dayton TL,Kim-Kiselak C,Hoersch S,Whittaker CA,Bronson RT,Beer DG,Winslow MM,Jacks T

    更新日期:2013-07-15 00:00:00

  • p27kip1 independently promotes neuronal differentiation and migration in the cerebral cortex.

    abstract::The generation of neurons by progenitor cells involves the tight coordination of multiple cellular activities, including cell cycle exit, initiation of neuronal differentiation, and cell migration. The mechanisms that integrate these different events into a coherent developmental program are not well understood. Here ...

    journal_title:Genes & development

    pub_type: 杂志文章


    authors: Nguyen L,Besson A,Heng JI,Schuurmans C,Teboul L,Parras C,Philpott A,Roberts JM,Guillemot F

    更新日期:2006-06-01 00:00:00

  • KSR modulates signal propagation within the MAPK cascade.

    abstract::Kinase suppressor of Ras (KSR) is a recently identified component of Ras-dependent signaling pathways. In this report, we show that murine KSR1 (mKSR1) cooperates with activated Ras to promote Xenopus oocyte maturation and cellular transformation and provide evidence that this cooperation occurs by accelerating mitoge...

    journal_title:Genes & development

    pub_type: 杂志文章


    authors: Therrien M,Michaud NR,Rubin GM,Morrison DK

    更新日期:1996-11-01 00:00:00

  • The Med proteins of yeast and their function through the RNA polymerase II carboxy-terminal domain.

    abstract::Mediator was resolved from yeast as a multiprotein complex on the basis of its requirement for transcriptional activation in a fully defined system. Three groups of mediator polypeptides could be distinguished: the products of five SRB genes, identified as suppressors of carboxy-terminal domain (CTD)-truncation mutant...

    journal_title:Genes & development

    pub_type: 杂志文章


    authors: Myers LC,Gustafsson CM,Bushnell DA,Lui M,Erdjument-Bromage H,Tempst P,Kornberg RD

    更新日期:1998-01-01 00:00:00

  • GCN2 sustains mTORC1 suppression upon amino acid deprivation by inducing Sestrin2.

    abstract::Mammalian cells possess two amino acid-sensing kinases: general control nonderepressible 2 (GCN2) and mechanistic target of rapamycin complex 1 (mTORC1). Their combined effects orchestrate cellular adaptation to amino acid levels, but how their activities are coordinated remains poorly understood. Here, we demonstrate...

    journal_title:Genes & development

    pub_type: 杂志文章


    authors: Ye J,Palm W,Peng M,King B,Lindsten T,Li MO,Koumenis C,Thompson CB

    更新日期:2015-11-15 00:00:00

  • Loss of Apc in vivo immediately perturbs Wnt signaling, differentiation, and migration.

    abstract::Although Apc is well characterized as a tumor-suppressor gene in the intestine, the precise mechanism of this suppression remains to be defined. Using a novel inducible Ahcre transgenic line in conjunction with a loxP-flanked Apc allele we, show that loss of Apc acutely activates Wnt signaling through the nuclear accu...

    journal_title:Genes & development

    pub_type: 杂志文章


    authors: Sansom OJ,Reed KR,Hayes AJ,Ireland H,Brinkmann H,Newton IP,Batlle E,Simon-Assmann P,Clevers H,Nathke IS,Clarke AR,Winton DJ

    更新日期:2004-06-15 00:00:00

  • Induction of cell fate in the Drosophila retina: the bride of sevenless protein is predicted to contain a large extracellular domain and seven transmembrane segments.

    abstract::Previous genetic mosaic studies established that expression of the Drosophila bride of sevenless (boss) gene is required in photoreceptor neuron R8 for the development of photoreceptor neuron R7. This led to the proposal that boss encodes or regulates an R7-specific inductive cue. We have identified the boss gene base...

    journal_title:Genes & development

    pub_type: 杂志文章


    authors: Hart AC,Krämer H,Van Vactor DL Jr,Paidhungat M,Zipursky SL

    更新日期:1990-11-01 00:00:00

  • Transcription elongation factor hSPT5 stimulates mRNA capping.

    abstract::RNA polymerase II nascent transcripts are capped during pausing before elongation. Here we report that hSPT5, the human homolog of yeast elongation factor SPT5, interacts directly with the capping enzyme. hSPT5 stimulated capping enzyme guanylylation and mRNA capping by severalfold. Although RNA 5'-triphosphatase acti...

    journal_title:Genes & development

    pub_type: 杂志文章


    authors: Wen Y,Shatkin AJ

    更新日期:1999-07-15 00:00:00