Abstract:
:The ultraviolet (UV)-induced (6-4) pyrimidine-pyrimidone photoproduct [(6-4) PP] confers a large structural distortion in DNA. Here we examine in human cells the roles of translesion synthesis (TLS) DNA polymerases (Pols) in promoting replication through a (6-4) TT photoproduct carried on a duplex plasmid where bidirectional replication initiates from an origin of replication. We show that TLS contributes to a large fraction of lesion bypass and that it is mostly error-free. We find that, whereas Pol eta and Pol iota provide alternate pathways for mutagenic TLS, surprisingly, Pol zeta functions independently of these Pols and in a predominantly error-free manner. We verify and extend these observations in mouse cells and conclude that, in human cells, TLS during replication can be markedly error-free even opposite a highly distorting DNA lesion.
journal_name
Genes Devjournal_title
Genes & developmentauthors
Yoon JH,Prakash L,Prakash Sdoi
10.1101/gad.1872810subject
Has Abstractpub_date
2010-01-15 00:00:00pages
123-8issue
2eissn
0890-9369issn
1549-5477pii
24/2/123journal_volume
24pub_type
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journal_title:Genes & development
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journal_title:Genes & development
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pub_type: 杂志文章
doi:
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journal_title:Genes & development
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更新日期:2000-01-01 00:00:00
abstract::FISH analysis of well-spread chromosomes reveals that homologs are paired in vegetatively growing budding yeast diploid cells, via multiple interstitial interactions, and independent of recA homologs and mating type heterozygosity. Pairing is present during G1 and G2, and in cells arrested at G1 by mating pheromone, b...
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journal_title:Genes & development
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journal_title:Genes & development
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journal_title:Genes & development
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