Abstract:
:Eukaryotic chromosomes reach their stable rod-shaped appearance in mitosis in a reaction dependent on the evolutionarily conserved condensin complex. Little is known about how and where condensin associates with chromosomes. Here, we analyze condensin binding to budding yeast chromosomes using high-resolution oligonucleotide tiling arrays. Condensin-binding sites coincide with those of the loading factor Scc2/4 of the related cohesin complex. The sites map to tRNA and other genes bound by the RNA polymerase III transcription factor TFIIIC, and ribosomal protein and SNR genes. An ectopic B-box element, recognized by TFIIIC, constitutes a minimal condensin-binding site, and TFIIIC and the Scc2/4 complex promote functional condensin association with chromosomes. A similar pattern of condensin binding is conserved along fission yeast chromosomes. This reveals that TFIIIC-binding sites, including tRNA genes, constitute a hitherto unknown chromosomal feature with important implications for chromosome architecture during both interphase and mitosis.
journal_name
Genes Devjournal_title
Genes & developmentauthors
D'Ambrosio C,Schmidt CK,Katou Y,Kelly G,Itoh T,Shirahige K,Uhlmann Fdoi
10.1101/gad.1675708subject
Has Abstractpub_date
2008-08-15 00:00:00pages
2215-27issue
16eissn
0890-9369issn
1549-5477pii
22/16/2215journal_volume
22pub_type
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